Gcc2 (NM_001107633) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Lims1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213995 representing NM_001107633 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATTCTGCTCAAGATGCTGTGACTGCAGCACCCTCCGGGACCCCGAAATCCAAGCTAGAAACAT TGCCCAGAGAAGATCTCATCAAGTTTGCCAAGAAGCAGATGATGCTCCTACAGAAAGCCAAAGCAAGGTG TACAGAATTAGACAAAGAAGTTGAAGAGCTCAAATCAAAACCTGTTGACGGAGGAACTGATGATATGATT AAGGTGCTAACTGAACGCTTGGATGCTCTTCTTCTGGAAAAAGCAGAGACTGAACAACAGTGTCTGTGTC TCAAAAAAGAAAATATTAAAATGAAGCAAGAGGTTGAGGATTCTGTAACTAAACTGGAAGAGACACAAAA AGAGTTTGAACAATCACATAGAAACTACGTAAGAGAAATGGAAAGTGTTAAAAATGAACTGATAGCAGTA CACTCAGAGCACAGCAAAGAAAAGGCTGCCTTACAAAGAGACCTGGAGGGAGCAGTCCATAGGCAAGCAG AGCTGTTAGAACAGCTCAAGTCTCAGAGCGACTCTGAAGATAATGTTAAAAAACTACAGGAAGAGATTCA GAACATTACAGCAGCATTTGAGGAACAAACGTCATGTCTACAAAAGCAATTAGAAGCTACCAGTGATGAA AAACAGCAAGAGATTATTCATCTCCAGAAAGTCATTGAGGACAACGCTCAGCACTACCAAAAAGATATTA ATACTTTCCAAGAAGAGATTGTGCAGCTGAGAGCTACACACAAAGAAGAAGTTAATGAGCTGATGTCTCA AATGGAAACATTAGCTAAAGAACATGAGGCAGCAGTAAATAAGCTAAAAGAGAACAGAGTGACACTGTGT GAGACAAGTGAGACCATTCCAGAGAATTACCAATGTGAATCAGAAAGCTTAAATGAAGACACCTCAGATG CAAGCCAGGAGAATCAAAAATGCTCTGTGGCCTTACAGGAAGATCCTTTTGCAGAACATACAGTGTATGA TAAAGTCAGACAATTGGAAGATTCATTAAAAGAACTGGAATCTCAACATAGTATCTTAAAAGATGAAGTG ACTTATATGAATAATCTCAAGTTAAAACTTGAAATGGATGCCCAGCATATAAAGGATGAGTTTTTCCATG AAAGAGAAGACTTGGAGTTTAAAATTAATGAACTGTTGCTGGCCAAAGAAGAACAGAGCTACGTAGTAGA AAAATTAAAATATGAGCGAGAAGATTTAAATAGGCAGCTTTGCTGTACTGTGGAACAACATAACAAAGAA ATACAGCGTCTTCAGGAGCACCATCAGAAAGAAATCTCTGAACTAAGTGAGACATTTATGTCAGGTTCAG AAAAAGAAAAGTTAGCATTAATGTTTGAAATACAGGGACTTAAGGAACAGTGTGAAAATCTACAACATGA AAAGCAAGAGGTAGTTCTAAATTATGAGAGTTTACGAGAGATGATGGAAATTTTACAAACGGAACTTGGA GAATCTGCTGGAAAAATAAGTCAAGAGTTTGAAACAATGAAGCAACAGCAAGCATCTGATGTTCATGAGC TTCAGCAGAAGCTAAGAACTGCTTTTAACGAAAAAGACGCTCTTCTTGAAACTATAAATCGCCTACAAGG AGAAAATGAAAAATTATTATCACAAGAATTGGTATCAGAACTCGAAAGTACCATGAAGAACCTTAAGGCA GATAATAGCATGTACTTAGCCAGCCTTGGTCAAAAAGATACCCTGTTACAAGAATTAGAAGCAAAGATAA GTTCTCTTGCTGAAGAAAAAGATGATTTTATAAGTAAAATCAAAACTTCCCGTGAAGAGATAGATGATCT CCATCAGAAATGGGAAAGGGAACAGAAACTGTCTGTAGAACTCAGGGAAGCAGCAGAGCAAGCCGCCCAG CACAACAGTGAACTGAGACAAAGAGTAAGTGAATTAACAGGAAAACTAGATGAAATATTAAGAGAAAAGA GTCAAAATGACCAAAACATTATGGTTCAAATGAAAACCATGACAGAAGACCAAGAAGCATTGTCATCTAA AATCAAGTCTCTTTATGAGGAAAACAATAGACTGCATTCTGAAAAGGTCCAGTTGAGTAGAGATTTAGAA GCTCTTCAGTCTCAACAAGATTTTGCCTATAAAGAGCATGTTGCTGAATTCGAAAAGAAACTCCAGTTAA TGGTTGAAGAACGAGATGATTTAAATAAACTGCTTGAAAATGAGCAACTTCAGAAGTCATTTGTCAAAAC TCAGTTGTACGAGTTTCTTAAGCAAATGAGGCCAAGCATTTTAGAAGATAATGAAGAGGAAGATGTTGTC ACAGTTCTAAAAGCTGTGGGTGAATCCTTAGTGACAGTAAAGGAAGAAAAGCATAACTTAGTCTTTGAAT ATGATGCGAGAGTATTAGAATTAGAAAGGAGGATTAAGTGCCTTCAAGAGGAGAGTGTAGTTCAGTGTGA AGAACTAAGGGCTTTAGTAAGAGACTCTGAACAAGAGAAAATTCTCCTAAGAAAAGAATTAGATGAAGTC ACATCAACAAAAGAGGCCCTGCAGTGTGATATTCTAGAAATGAAGAATACTAATGAAAAAACAAGCCTTG AAAATCAGACTCTTTCAACTCGGGTTGAAGAATTATCGCGGTCATTACACAGCAAAAATGAAGTCCATAA TGAAAAGGATCTTGTCATAGAACATGAAAACCTGAGGCTATCGCTTGAGCAGAGGGAGTCTGAGCTACAA GATGTGAGAGCAGAATTGATGCTGCTGAAGGATTCCTTAGAGAAATCGCCTTCTGTAAAAAATGATCAAC TTTCATTGGTAAAAGAGCTGGAAGAAAAAATAGAAAGTCTGGAAAAAGAATCCAAAGACAAGGATGAGAA AATCAGTAAGATAAAACTAGTCGCTGTGAGAGCAAAGAAAGAACTAGATTCTAACAGGAAAGAGGCCCAG ACACTACGGGACGAACTCGAGTCCGTACAGTCAGAAAAGGACCGCTTATCTGCTTCTATGAAGGAGTTTA TCCAAGGAGCAGAAAGCTATAAGAATCTTCTGTTAGAATATGACAAGCAGTCAGAACAGCTGGATGTGGA AAAAGAACGTGCTAATAATTTTGAACATCACATAGAAGACCTTACCAAACAATTAAGAGATTCGACTTGC CAGTATGAAAAACTAACTTCCGACAATGAGGATCTCCTGGCTCGAATTGAGACCCTGCAGGCTAATGCCA GGTTATTAGAAGCACAGATCTTAGAGGTCCAGAGAGCCAAAGGAGTAGTAGAAAAAGAACTGGAAGCTGA AAAACTTCAGAAAGAGCAGAAAATTAAGGAACATGTCAGTACTACAAATGAGCTTGAAGAACTTCAACTC CAATTTCAAAAGGAAAAGAAGCAGCTTCAGAAAACGATGCAAGAATTAGAACTGGTTAAAAAGGATGCCC AACAGACCACGTTGATGAATATGGAAATAGCAGATTACGAACGTTTGATGAAAGAATTAAATCAAAAGTT AACAAATAAAAACAGTAAGATAGAAGATTTGGAGCAAGAAATGAAAATTCAGAAACAGAAACAAGAAACC CTACAAGAAGAAATGACTTCCCTGCAGTCTTCAGTACAGCACTACGAAGAAAAGAACGCCCAAATCAAGC AGCTGCTTGTGAAAACCAAAAAGGAACTGGCAGATGCAAAGCAAGCAGAAACTGATCACTTACTACTCCA GGCGTCTTTAAAGGGCGAGCTGGAAGCAAGCCAGCAGCAAGTAGAAGTCTATAAAATTCAGCTAGCTGAG ATGACATCTGAGAAACACAAAATCCATGAGCACCTGAAGACTTCTGCGGAACAGCACCAGCGCACACTAA GTGCATACCAGCAGAGGGTGGTGGCCCTGCAGGAAGAGAGCCGCACTGCCAAGGCAGAGCAAGCTGCTGT CACCTCTGAATTTGAGAACTACAAAGTCCGGGTTCATAACGTTCTAAAACAACAGAAAAATAAATCTGTG TCTCAGGCTGAAACTGAGGGAGCTAAACAAGAAAGGGAACACCTGGAAATGCTGATTGACCAACTGAAAA TCAAGTTACAGGACAGCCAAAACAGCTTACAAATCAGTGTGTCAGAGTTCCAGACACTGCAGTCTGAGCA TGACACTCTGCTAGAGAGGCACAACCGGATGCTGCAGGAAACTGTGACCAAAGAGGCGGAACTTCGGGAA AAGTTATGTTCAGCTCAATCTGAGAACACCATGTTGAAGTCTGAACACGCCCAGACAATGTGTCAGCTGA CATCCCAGAATGAGGCCCTCCGTAACAGCTTCCGAGACCAAGTTCGACACTTGCAGGATGAGCACAGAAA GACAGTGGAGACCTTGCAGCACCAGCTCTCCAAGGTGGAAACTCAGCTCTTCCAGCTCAAGAGTGAGCCA CCCACAAAAAGCCCTGCTTCTTCCCATCAACCTTCAAAAAGCCTCCGAGAAAGACGGACCACAGACCTCC CCCTTCTGGACATGCACACTGTGACCCGAGAGGAGGGGGAAGGGATGGAGACAACTGACAGTGAGTCTGT GTCTTCGGCCGGCACACACATTCAGTCCTTGGAGCAGCTCCTTAGCTCTCCTGACAGCAAGCTTGAACGT CTTACAGAGGCCTCCTTATGGCATACAGAATTTACCAAAGAAGAATTGGCTGAGAAGCTCAGTTCCACCA CGAAGAGTGCAGATCACTTAAATGGCCTGCTTCGGGAGACAGAGGCTACCAACGCCATCCTTATGGAGCA AATTAAGCTTCTCAAAAGTGAAATAAGAAGACTGGAAAGAAACCAAGAGCGGGAGAAGTCTGTGGCTAAC CTGGAGTACTTGAAGAATGTCCTACTGCGGTTCATTTTCCTGAAGCCGGGTAGTGAAAGAGAGAGGCTTC TTCCTGTGATAGACACGATGCTGCAGCTCAGCCCTGAAGAAAAGGGAAAGCTTGCCACAGTTGCTCAAGG TGAGGAAGAAAGTGCCTCTCGTTCCTCTGGATGGGCATCCTACCTTCATAGCTGGTCTGGACTGCGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107633
Insert Size	5040 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107633.1, NP_001101103.1
RefSeq Size	6526 bp
RefSeq ORF	5040 bp
Locus ID	309798
UniProt ID	D3ZZL9
Gene Summary	Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2 (By similarity).[UniProtKB/Swiss-Prot Function]

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