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Aloxe3 (NM_001105793) Rat Untagged Clone
CAT＃: RN213879
Aloxe3 (untagged ORF) - Rat arachidonate lipoxygenase 3 (Aloxe3), (10 ug)
CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | e-LOX-3; eLOX-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213879 representing NM_001105793
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTATATCGCCTGTGTGTGACCACTGGTTCCTACCTGAAGGCTGGCACATTGGATAACATCTATG CTACACTGGTAGGCACCTGTGGTGAAAGCCCTAAGCAGAAGCTGGATCGTGTAGGCAGGGACTTCGCCAC CGGATCGGTTCAGAAGTACAAGGTGCGCTGTGCATCAGAGCTGGGTGAGATCTTGCTGTTGCGCTTACAC AAGGAGCGCTTTGCTTTCTTCTGCAAGGATCCTTGGTACTGCAGTCGCATCTGTGTCACTACCCCAGATG GCTCTGTGGTTCACTTTCCCTGTTATCAGTGGATCGATGGCTACTGTACTGTGGAGCTTCGGCCAGGAAC AGCAAGAACCATCTGCCAGGATGCTCTTCCCCTCCTTCTGGATCACAGGAAGCGTGAACTCCAAGCCCGC CAAGAATGCTACCGCTGGAAGATCTATGCCCCTGGTTTCCCTCGAATGGTGGATGTCAGCAGCTTTGAGG AGATGGAGTCAGATAAGAAATTTGCCTTGACCAAGACGGCGCCTTGTGCAGACCAGGATGATAACTCTGG GAACCGGTACCTGCCTGGCTTTCCTATGAAAGTTGACATCCCATCTCTGCTGCACATGGAGCCCAACATT CGCTACTCAGCCACCAAGACAGCCTCACTCATCTTCAACGCCCTTCCTGCATCCTTGGGCATGAAGATTC GAGGTCTGCTAGACCGCAAGGGCTCCTGGAAGAGGCTGGATGACATCCGGAACATCTTCTGGTGTCATAA GACCTTCACTTCAGAGTATGTCACCGAGCACTGGTGTGAGGACAGCTTCTTTGGGTACCAGTACCTGAAC GGTGTCAATCCCATCATGCTCCACTGCCTCTCCAGTCTGCCCAGCAAGCTGCCTGTCACCAATGACATGG TGGCACCTTTGCTGGGACCCGGCACCTGCCTGCAAACAGAGCTAGAGAGGGGGCACATCTTCTTAGCGGA CTACTGGATCCTGGCGGAGGCCCCAGTCCATTGCCTAAACGGCCGCCAACAGTACGTAACAGCTCCTCTC TGCCTGTTATGGCTCAACCCACAGGGGGTGCTGCTGCCGTTGGCAATCCAGCTCAGCCAGATACCAGGGC CAGAGAGCCCCATCTTTCTACCCACTGACTGCGAGTTGGATTGGCTGCTGGCCAAGACGTGGGTGCGCAA CTCTGAGTTTTTGGTGCATGAGAACAACACGCATTTTCTGTGCACGCATTTGCTCTGCGAGGCCTTCTCC ATGGCTACACTGCGTCAGCTGCCCCTCTGTCATCCAGTCTACAAGCTCCTGCTTCCTCACACTCGCTACA CACTGCAAGTGAACACCATCGCAAGGGCCACACTGCTCAACCCAGACGGCCTCGTGGACAAGGTCACATC CATCGGTAGGCGCGGCCTCATCTACCTCATGAGCACAGGGCTGGCCCACTTCACCTACACGGATTTCTGC CTTCCGGATAGCCTACGGGCTCGTGGTGTCCTGACCATTCCCAACTACCACTACCGAGACGACGGCCTCA AGATCTGGGCGGCTATTGAGAGGTTTGTCTCCGAGATTGTGAGTTACTATTACCCCAACGATGCCTGTGT GCAGCAGGACTCTGAGCTGCAGGCCTGGGTGGGTGAGATTTTTGCTCAGGCATTCCTCGGTCGGGAGAGT TCAGGCTTCCCAAGCAGGCTGTGCACCCCAGGAGAGCTCGTGAAGTATCTCACTGCAATCATCTTTAACT GCTCCGCCCAGCACGCTGCAGTCAACAGTGGGCAGCACGACTTTGGGGCCTGGATGCCCAATGCCCCGTC ATCCATGAGGCAGCCTCCACCTCAGACCAAGGGCAACACGACAATGGAGAGTTACCTCGAAACCCTCCCA GAGGTGAACACCACCTGTAGCAACCTTCTCCTCTTCTGGCTGGTCAGCCAAGAGCCAAAGGACCAGAGGC CTCTGGGCACCTACCCAGACGAACACTTCACAGAGGAGGCCCCACGGCAAAGCATCGCAGCCTTCCAGAA GTGCCTGGCACAGATCTCAAAGGACATCAGGGCACGCAACGAGAGCCTGGCACTACCCTATGCCTACTTG GATCCACCACTCATTGAGAACAGTGTCTCCATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001105793 |
| Insert Size | 2136 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001105793.1, NP_001099263.1 |
| RefSeq Size | 2525 bp |
| RefSeq ORF | 2136 bp |
| Locus ID | 287424 |
| UniProt ID | D3ZKX9 |
| Gene Summary | Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activates PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213879 | Aloxe3 (Myc-DDK-tagged ORF) - Rat arachidonate lipoxygenase 3 (Aloxe3), (10 ug) |
CNY 9,120.00 |
|
| RR213879L3 | Lenti ORF clone of Aloxe3 (Myc-DDK-tagged ORF) - Rat arachidonate lipoxygenase 3 (Aloxe3), (10 ug) |
CNY 10,930.00 |
|
| RR213879L4 | Lenti ORF clone of Aloxe3 (mGFP-tagged ORF) - Rat arachidonate lipoxygenase 3 (Aloxe3), (10 ug) |
CNY 12,070.00 |
