Sec31b (NM_001135713) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213829 representing NM_001135713 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTGAAGGAGCTTGAGAAGCCAGCTGTCCAAGCATGGAGCCCAGCGAAGCAGTACCCTGTGTATC TGGCCACAGGAACATCCGCCCAGCAACTAGATGCCTCCTTCAGCACAAATGCCACCCTGGAAATATTTGA GGTTGATTTCAGGGACCCTTCTCTGGACTTGAAACGCAAAGGAATCCTCTCTGTGTCAAGCAGGTTTCAC AAGCTAATCTGGGGAAGCTCCAGCAGTGGGCTTCTGGAGAACACTGGGGTTATTGCTGGTGGAGGAGACA GTGGCATGCTTACCCTATACAATGTGACCCACATCCTTTCTCCGGGGAAGGAACCCTTGATTGCCCAGAA ACAGAAGCACACAGGAGCTGTCAGAGCCTTGGACTTTAATCCTTTCCAGGGCAACCTCCTGGCCTCAGGA GCCAGTGATTCTGAAATCTTCATTTGGGATTTGAACCACCTGACTGTTCCAATGACACCTGGATCCAAGT CACAGAACCCCCCGGAAGACATTAAAGCATTGTCTTGGAACCGCCAAGTCCAGCACATTCTGTCTTCTGC TCACCCCAGTGGCAAGGCAGTTGTGTGGGATCTCAGGAAGAACGAGCCCATCATCAAAGTCAGCGACCAC AGCAGCAGGATGAACTGCTCAGGCCTGGCCTGGAACCCAGACATAGCCACACAGTTAGTGCTGTGCTCAG AAGATGACCGGCTCCCAGTGATTCAGCTGTGGGACCTGCGCTTCGCTTCCTCACCCCTGAAGGTGTTGGA GAGCCACAGCAGGGGCATCTTGTCGGTGTCCTGGAGCCAGGCTGATGCTGAACTGCTGCTTAGCAGTGCC AAAGACAACCAGATTTTTTGCTGGAATCTGTCAAGCAGTGAGGTGGTCTACAAGCTACCCACACAGAGCA GCTGGTGCTTTGACGTTCAGTGGTGCCCTCGGAACCCTCCTGCGTTCTCTGCTGTCTCCTTCGATGGCTG GATCAGTTTGTACTCTGTGATGGGTAGGAGCTGGGAAGCCCAGCAGCACATGAGGCAGGCTGACAAGATT TCCTCTTCCTTTAGCCAAGGCCAGCCTCTCCCACCACTGCAGGTGCCTGAGCAGGTGGCCCAAGCATCAC TAATTCCTCCCTTGAAGAAGCCTCCCAAGTGGATGAGAAGGCCGGCAGGCGTTTCATTCGCTTTTGGGGG AAAGCTGGTTACCTTTGGCCTTCCCAGCATCCCTGTCCAGCAGGTGCCACAGCCTTGTCCCCGCCCTGTC TTCATCAGTCAAGTCATCACAGAGTCAGAATTCCTGACTCGGTCAGTTGTGCTGCAGGAGGCCTTGGGAT CTGGAAATCTCCTTAATTATTGTCAGAGCAAGATTCAGCAAGCTTCACGGCCAAGTGAGAAGACGCTCTG GCAGTTTCTGAAGGTGACCTTGGAGCATGATTCCAGACTGAAGTTCCTCAGGCTGTTAGGTTACAGCAGA GATGAACTTCAGAAGAAGGTGGACACATGCCTGAAGAGTGACTCGAAATCACAGGAGAGCCCTCAGCTTG AGGCAGTTGACCTCAAAAGTGACAGAGCACACACCCTTTGCAGCCAGGCCTCCAGACACACTGCCAAGGA AGCCTCTGCATCCTCAGCCTTCTTTGATGAGCTGATACCTCAGAACATGACTCCCTGGGAGATCCCCACC ACAGAAGACACTGATGGGCTCCTGAGCCAGGCCCTGCTGCTCGGGGAACTGCGGTCTGCTGTGGAGCTCT GTCTGAAAGAGGAACGCTTTGCTGACGCCATCATCCTGGCTCAGGCTGGGGACGCAGAGCTACTCAAGTG GACACAAGAACGATACCTAGCCAAGAGGAGAACCAAAACCTCCTCGCTTCTAGCCTGTGTCGTAAAGAAG AATTGGAAGGACCTAGTATGTGCCTGTAGCCTGAAGAACTGGAGAGAGGCGCTGGCCTTGCTGCTGACGT ATTCAGGGCCAGAGAAGTTCCCTGAGCTCTGTGACATGCTGGGAGCTCGCATGGAGCACGAGGGAGGCAG GGAGCTCACCTCTGAAGCCAGGCTGTGCTATGTGTGCTCAGGGAGTGTGGAGCGGCTGGTAGAGAGCTGG GCAGACTCCCAGCAAGCCTCGAGCCCCGTGGCTCTGCAGGAGCTAATGGAGCAGGTGACGGTCCTCAGCA GGAGCCTGGAGCTCCTCCAGGGTTCTCACAAGATGAGCCCAGGTCCTGCCACAACACACAGGTTCACCCA GTATGCCAGTCTCCTGGCAGCCCAGGGCAGCCTGGCCATTGCCATGAGTGTCCTACCCAGTGACTGTACC CAGCCCGCAGTCCTGCAGCTGAAAGATCGGCTTTTCCATGCCCAAGGTTCTACCGTCTTGGGCCGACAGG CTCCTGCTTTCCCGTTTCCCCGCGTTGCTGTAGGAGCTGCTCTCCACCCAAAAGAGACATCATCACACAG AATGGGATTCCAGCCTCCTCGCCAGGTCCCAGCTCCATCTGTGAGGCCGAGGGCCGCAGCTCAGCCGTCA GTGATGCCTTTCCTCCCCTCCCATCCCATCCCTTCTGTGGGCTCGTGGACACAGTCCTCAAGTGACTACA GGGTCCCCAAACCCCAAGCAACCTTGCCTGTGCATTTCGTCCCTGGAGTAAGGCCTGCTTTCTCTCAGCC ACAGCCATTCGGAGGACAAAGTGTCCAAGCTATAAACCCTGTTGGATTCTGTGGAACATGGCCTCTTCCT GGTCCTACTCCAGTCATGGCACCCCCAGATGTCATGCAGCCTGGCTCTACCCACCTGCCTGAAACTCCTA GACTGCTCCCTCTGCCTCCTGTGGGACCACCAGGTCCCACCCCTCTGAGCTCCCAACCTGCAGCCTCTCC TGTCACTTTCTCTGTGGCTCACCCTCCTGGCGGCCCTGGAGCACCACGCTCTAGTGCCCTCCCGAGCTCT GGCATCTTGGCCACTCGTCCAGGACCCCAGGATACCTGGAAAGTTGCTCCAGCCTCCCAGGAAAACCTCC AAAGGAAAAAGTTGCCTGAGACATTTATGCCTCCAGCACCAATAATTACAGCTCCACTTATGAGCCTTGG CCCTGAGCCACAACAGGCCCTGCTGCCACAGTCCCTAGTCTCCGGTGCAAGCCTGCCTCCTCCTGGAGCC CCGAGAGAATGCAGCCTGCAGCAACTCCAGCCACTGCCCCCTGAGAAGACACAGAAAGAGCTGCCCCCAG AGCATCAGTGCCTGAAGGACAGCTTTGAGGCGCTCCTTCAGCGCTGTTCCCTGACCGCCACTGACCTAAA AACAAAAAGGAAGCTGGATGAGGCAGCCCACCGTCTAGAATGTCTATACGAGAAGCTGTGCGAGGGGACA CTCTCACCCCATGTCCTGGCTGGGCTCCATGAAGTCGCCCGCTGTGTGGATGCAGGAAGCTTTGAACAGG GCCTTGCAGTGCATGCCCAGGTAGTGAGCTGCAGCAGCTTCAGCGAGGTATCCAGCTTCATGCCTGTCCT GAAGGCTGTCCTCACCATCGCGCACAAGCTGCAGGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135713
Insert Size	3540 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135713.1, NP_001129185.1
RefSeq Size	3625 bp
RefSeq ORF	3540 bp
Locus ID	309433

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