Magi3 (NM_139084) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Slipr
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213790 representing NM_139084 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAAGACGTTGAAGAAGAAGAAGCATTGGCTCAGCAAGGTGCAGGAGTGTGCGGTGTCCTGGGCCG GGCCCCCGGGCGACTTGGGCGCTGAGATCCGCGGCGGCGCGGAGCGCGGCGAGTTCCCTTACCTGGGGCG GCTCCGTGACGAGCCTGGCGGCGGCGGAGGCACCTGCTGCGTGGTCTCGGGCAAGGCGCCCAGTCCTGGC GATGTGCTGTTGGAGGTGAACGGGACGCCCGTCAGTGGGCTCACCAACCGGGACACCCTGGCTGTCATCC GCCACTTCCGCGAGCCAATCCGTCTGAAGACCGTGAAGCCAGGCAAAGTCATAAATAAAGATTTGCGACA TTACCTGAGTCTGCAGTTTCAGAAAGGATCGATTGACCACAAACTACAGCAGGTCATCAGAGATAACCTC TACTTGATAACCATCCCATGCACTACAAGGGCTCCCAGGGATGGGGAAGTTCCAGGGGTGGACTATAATT TCATTTCTGTTGAACAATTCAAGGCACTGGAAGAAAGCGGAGCATTGTTAGAAAGTGGAACGTATGATGG AAATTTCTATGGCACGCCCAAGCCTCCAGCAGAGCCTAGTCCTTTCCAGCCCGATCCAGTTGATCAGGTC CTCTTTGACAATGAGTTTGACACAGAATCCCAAAGAAAACGAACTACATCTGTCAGCAAGATGGAAAGGA TGGACAGCTCTCTCCCTGAGGAAGAGGAAGATGAGGACAAAGAAGCTGTCAATGGCAGCGGAAGCATGGA AACTAGAGAGATGCATTCTGAGTCGTCTGACTGCTGGATGAAGACTGTTCCAAGTTATAACCAAACAAAT CGCTCCATGGACTTTAGAAATTATATGATGAGAGACGAGAATCTGGAGCCGCTGCCCAAAAACTGGGAAA TGGCCTACACTGACACAGGAACGATCTACTTCATTGATCACAATACGAAGACAACCACCTGGCTGGATCC TCGTCTCTGCAAGAAAGCCAAAGCCCCTGAAGACTGTGAGGATGGAGAGCTTCCATATGGCTGGGAGAAA ATAGAGGACCCTCAGTATGGAACATACTATGTTGATCACCTCAACCAGAAAACTCAGTTTGAAAATCCAG TGGAGGAAGCGAAAAGGAAAAAGCAGATAGGACAGGCTGAAACTCATTCCGCAAAAACAGATGTGGAAAG AGCACACTTTACCCGGGACCCATCCCAACTTAAAGGTGTCCTTGTTCGAGCATCACTGAAAAAAAGCACA ATGGGCTTTGGTTTCACCATTATAGGTGGAGATCGGCCTGATGAATTTCTACAAGTAAAAAATGTGCTCA AAGACGGTCCCGCTGCTCAGGATGGGAAAATGGCACCAGGTGATGTTATTGTAGACATCAATGGCAACTG TGTCCTTGGTCACACCCATGCAGATGTTGTCCAGATGTTTCAATTGGTACCTGTCAACCAGTATGTCAAC CTTACTTTATGCCGTGGTTATGCACTTCCTGACGACAGTGAAGACCCTGTTGTGGACATTGTTGCTGCTA CCCCTGTTATCAATGGACAATCATTAGCAAAGGGAGAGGCGTGCATGAGTACTCAGGATTTTAAACTGGG AGCAATGGTTTTGGATCAGAATGGAAAATCAGGAAAACTTTTGTCCAGTGATCGTCTCAATGGCCCATCA GATTCAAATGAGCAAAGAGCATCCCTGGCTTCATCAGGCAGCTCCCAGCCTGAACTAGTGACTATCCCGC TGGTTAAGGGTCCCAAAGGCTTCGGGTTTGCAATTGCCGATAGCCCAACTGGGCAGAAGGTGAAAATGAT ACTGGATAGCCAGTGGTGTCAAGGCCTTCAAAAAGGGGATATCATCAAAGAAATTTACCATCAGAATGTG CAGAATTTAACACATCTCCAAGTCGTAGAAGTACTGAAACAGTTCCCAGTAGGTGCAGATGTCCCGTTGC TTATCTTAAGAGGAGGTCCTTGTTCACCAACTAAAACTGCCAAAATGAAAACAGATACAAAAGAAACTTC AGGAAGTTTGGAGACTATAAATGAGCCTACTCCCCAGCCTATGCCTTTTCCACCCAGCATAATCAGATCA GGATCCCCGAAATTGGATCCTTCTGAAGTCTACCTGAAATCTAAGACTTTATATGAAGATAAACCACCCA ACACCAAAGACCTAGATGTCTTTCTTCGGAAACAAGAATCGGGGTTTGGCTTCAGGGTGCTGGGAGGAGA TGGACCTGACCAGTCTATATATATTGGGGCCATCATTCCCCTGGGAGCAGCGGAAAAGGATGGTAGGCTC CGTGCTGCTGATGAATTGATGTGTATTGATGGGATTCCTGTTAAAGGGAAATCACATAAGCAAGTCTTGG ACTTAATGACCACTGCTGCTCGAAATGGCCACGTGTTACTCACTGTGAGGAGGAAGATCTTCTATGGAGA GAAACAACCCGAGGATGAAAGCCCTCAAGCCTTCTCACAGAGTGGATCTCCTCGCCTAAATCGGACAGAG CTTCCAACCAGGTCTGCCCCACAGGAGTCCTATGATGTTATCTTACAGAGGAAAGAAAATGAAGGGTTTG GTTTTGTCATCCTCACCTCTAAAAGCAAGCCGCCCCCAGGAGTTATTCCTCATAAAATTGGCCGGGTCAT AGATGGAAGTCCAGCTGATCGTTGTGGAAGACTGAAAGTTGGAGACCACATCTCTGCGGTGAATGGGCAG TCCATTGTTGACCTGTCCCATGATAATATTGTTCAGCTCATCAAAGATGCTGGGGTCACCGTCACGCTGA CAGTGGTTGCTGAAGAAGAGCATCATGGTCCACCATCAGGAACAAACTCAGCCAGGCAGAGCCCAGCACT GCAGCACAGACCCATGGGACAGGCGCAGGCCACCCACATACCTGGGGACAGAACTGCCCTAGAAGGTGAA GTTGGGAAAGATGTCTGCAGCTCTTACAGACACTCCTGGTCTGACCACAAGCACCTTGCACAGCCGGACA CCGCGGTGATTTCCGTGGTGGGCAGTCGGCACAGTCAGAGCCTTGGGTGTTACCCTGTGGAGCTGGAGAG AGGCCCTCGAGGCTTTGGGTTCAGCCTCCGAGGAGGGAAGGAGTACAACATGGGGCTGTTCATCCTTCGC CTTGCTGAGGACGGGCCTGCCATCAAAGACGGCAGAATTCACGTTGGTGACCAGATCGTGGAAATCAATG GGGAACCTACACAAGGCATCACACACACGCGAGCCATTGAGCTCATTCAGGCTGGCGGGAATAAAGTCCT CCTCCTTCTGAGGCCAGGAACTGGCTTGATACCTGATCATGGTGATTGGGATATTTATAGTCCTTCATCT TCCAATGTCATTTATGATGAACAGCCACCACCTCTCCCCTCTTCACATTCTGCTGCCACATTTGAAGAGT CTCATGTGCCAGTAACTGAAGACTCTCTGATTAGAGTTCAGACATGTGAAAAGGCAGAAGAATTAAAGGA CACTGTACAAGAAAAGAAAAGCACTTTAAATGGAAGCCAGCCTGAGATGAAGTATCAGTCTGTCCAGAAA AATGTGAGTAAGAAGGATCCATCCAGAAGTCATGGGCATGGTGACAAGAATCTACCTAAAGGAGAAAATG GTGTTACACGGAGAGGTAGATCTGCTAGTCCCAAAAAGTCAGTTAATCGACATTCAGAGGAACATCTGGA AAAGATTCCCAGGCCCCTTAGAAGTGACCCCAAAGGAAAATCTAGAGACCGGTCACTCAGCCCTAGGAAA GGAGAAAATAAAGGTCAGGTCACCATTAAGGCGGGCTCTGGACAAGATCCCTGTAGAAAAGACAGAGGAC GGTCTTCTAGCCCCAGGAAGCAGCAAAAGATTGGAGGCAACAGCCTGTCAAACACTGAAGGCAAATTATC AGAGGCTGGGAGCCGAAGAGCAGCAGGCCTCTCTAGTGACAGCCCTGAACAGCTCCCAGAGGGGAAAGAA AAGTCAGGGGTCAGCAGGAAAGATCTGAAGCTCAGTCAGCTGGGGAAAAACAGAACAAGGTCTCCAGAGA AAAGGAGCAGCAAAGTTGATGAAGCGTCTCTCCCATCCAAAAAGACTAGTGACACTGCTAGTAGGGTAGT GTCTGAAAAGGAGAAAGGAAGGAAGCCAGGCACAGGAGAAAGAAGTAGAGACAAAACAGGAGAAAGCGTC CAGACCTCAGCAAAGCCGCTCACGCAGGAAGCGGGAGAGAAGATGGCATTAAGCAAAGCATCAGAGGTCA CTGACAGAGGCAAGGAGCGTGCAGGGGGCGCACCTGAGAGTAGCTCTCCAGTCAAGAAAGCACCCATCAC TCCAGGACCCTGGAGGGTGCCACGTGCAAATAAAGTCACAGGCACTGCTGGCATGGCTGACAAACAGCTG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_139084
Insert Size	4413 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_139084.2, NP_620784.2
RefSeq Size	5863 bp
RefSeq ORF	4413 bp
Locus ID	245903
UniProt ID	Q9JK71
Gene Summary	associates with tyrosine-phosphorylated proteins to link the receptor with its substrate in the plasma membrane [RGD, Feb 2006]

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