United States
Germany
Japan
United Kingdom
China
Mapkap1 (NM_001011964) Rat Untagged Clone
CAT＃: RN213619
Mapkap1 (untagged ORF) - Rat mitogen-activated protein kinase associated protein 1 (Mapkap1), (10 ug)
CNY 6,750.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213619 representing NM_001011964
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTCTTGGACAATCCAACTATCATTCTAGCTCATATTCGACAGTCACATGTGACCAGTGATGACA CGGGAATGTGTGAGATGGTTCTCATTGATCATGACGTTGACCTAGAGAAGACTCATCCTCCGTCAGTGCC TGGAGACAGTGGGTCAGAAGTTCAGGGAAGCAGTGGTGAGACGCAGGGCTACATATACGCCCAGTCTGTT GATATTACCTCGAGCTGGGACTTTGGTATTAGAAGACGCTCAAACACAGCTCAAAGATTAGAACGACTCC GCAAAGAGAGACAAAACCAGATCAAATGCAAAAATATTCAGTGGAAAGAAAGAAATTCTAAACAATCAGC CCAGGAGTTAAAGTCACTGTTTGAAAAAAAATCCCTCAAAGAGAAACCTCCAAGTTCGGGCAAGCAGTCC ATATTGTCTGTGCGCCTGGAACAGTGCCCTCTGCAGCTGAATAACCCCTTTAATGAGTACTCCAAGTTTG ATGGCAAGGGTCATGTGGGTACAACTGCAACGAAGAAGATCGACGTCTACCTCCCCCTGCACTCAAGCCA GGACAGACTGCTGCCAATGACTGTGGTGACCATGGCCAGCGCCAGGGTGCAGGACCTCATCGGGCTCATC TGTTGGCAGTACACGAGTGAAGGACGGGAGCCAAAGCTCAATGACAACGTCAGTGCCTACTGCCTGCATA TTGCTGAGGATGATGGGGAGGTGGACACGGATTTCCCACCGCTGGATTCCAATGAACCCATTCATAAGTT TGGCTTCAGTACTTTGGCCCTGGTTGAAAAGTACTCTTCCCCTGGTCTGACCTCCAAAGAGTCGCTCTTT GTTCGAATAAATGCTGCCCATGGGTTCTCCCTCATCCAGGTGGACAACACGAAGGTCACCATGAAGGAGA TCTTACTCAAGGCACTGAAACGAAGAAAAGGGTCCCAGAAAATTTCAGGCCCTCAGTACCGCCTGGAGAA GCAGAGCGAGCCTAACATCGGTGTGGACCTGGAGAGCACGCTGGAGAGCCAGAATGCCTGGGAGTTCTGC CTGGTTCGAGAGAACAGTTCAAGGGCAGACGGAGTTTTCGAGGAGGATTCACAAATTGACATTGCTACAG TGCAGGATATGCTTAGCAGCCACCATTATAAGTCATTCAAAGTCAGCATGATCCACAGACTGCGGTTCAC AACTGACGTGCAGTTAGGTATCTCTGGAGACAAAGTGGAGATAGACCCTGTTACGAATCAGAAAGCCAGC ACTAAGTTTTGGATTAAGCAGAAGCCCATCTCAATCGATTGTGACCTGCTCTGTGCCTGTGACCTTGCCG AGGAGAAGAGCCCCAGTCACGCGGTCTTTAAGCTCACGTATCTAAGCAGTCACGACTATAAGCATCTCTA CTTCGAGTCCGACGCAGCTACCGTCAGTGAAATCGTGCTCAAGGTTAACTACATCCTGGAGTCACGAGCA AGCACTGCCCGTGCTGACTATTTTGCTCAAAAACAAAGAAAACTGAACAGGCGGACGAGCTTCAGCTTCC AGAAGGAGAAGAAATCAGGGCAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001011964 |
| Insert Size | 1569 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001011964.1, NP_001011964.1 |
| RefSeq Size | 2400 bp |
| RefSeq ORF | 1569 bp |
| Locus ID | 296648 |
| UniProt ID | Q6AYF1 |
| Gene Summary | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-421'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213619 | Mapkap1 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase associated protein 1 (Mapkap1), (10 ug) |
CNY 6,750.00 |
|
| RR213619L3 | Lenti ORF clone of Mapkap1 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase associated protein 1 (Mapkap1), (10 ug) |
CNY 8,650.00 |
|
| RR213619L4 | Lenti ORF clone of Mapkap1 (mGFP-tagged ORF) - Rat mitogen-activated protein kinase associated protein 1 (Mapkap1), (10 ug) |
CNY 9,500.00 |
其它Mapkap1产品
