Smc3 (NM_031583) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	bamacan; Cspg6; SMC-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213612 representing NM_031583 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACATCAAGCAGGTGATTATCCAGGGCTTCCGAAGTTACCGAGATCAAACAATTGTAGATCCCTTCA GTTCAAAACATAATGTTATTGTGGGCAGAAATGGATCTGGAAAAAGCAACTTTTTTTATGCAATCCAGTT TGTTCTCAGTGATGAATTTAGTCATCTTCGTCCAGAGCAGCGATTGGCTTTGTTGCACGAGGGTACAGGT CCTCGTGTTATTTCTGCTTTTGTGGAAATCATTTTTGACAATTCAGACAACCGGTTACCAATTGATAAAG AGGAAGTTTCGCTTCGAAGAGTTATTGGTGCCAAAAAAGATCAGTATTTCCTAGATAAGAAGATGGTCAC GAAAAATGATGTGATGAATCTCCTTGAAAGTGCTGGGTTTTCGAGAAGTAATCCTTATTACATTGTTAAA CAAGGAAAGATCAACCAAATGGCAACAGCACCGGATTCTCAGAGATTAAAGCTGTTGAGAGAAGTAGCTG GCACTCGAGTGTATGACGAGCGCAAAGAGGAAAGCATCTCCCTGATGAAAGAAACAGAGGGCAAACGGGA AAAGATCAATGAGTTGTTAAAATACATTGAAGAGCGATTACATACTCTAGAGGAGGAGAAGGAGGAACTG GCCCAGTATCAGAAGTGGGATAAAATGAGGCGTGCCCTGGAGTACACCATCTACAACCAGGAGCTCAACG AGACTCGCGCCAAGCTTGATGAGCTTTCTGCTAAGCGAGAAACAAGCGGAGAGAAATCCAGACAATTAAG AGATGCCCAGCAGGATGCAAGAGATAAAATGGAGGATATTGAGCGCCAAGTTAGAGAACTGAAAACAAAA ATTTCAGCCATGAAAGAAGAAAAAGAACAGCTCAGTGCGGAGAGACAAGAGCAGATTAAGCAAAGGACCA AGCTGGAGCTTAAAGCCAAGGACTTACAGGATGAGCTGGCAGGGAACAGTGAGCAGCGGAAACGTTTATT AAAAGAAAGACAGAAGCTGCTTGAAAAAATAGAAGAAAAGCAGAAAGAACTGGCAGAAACAGAACCGAAA TTCAACAGCGTAAAGGAAAAAGAAGAGCGAGGAATTGCTAGATTGGCTCAAGCTACACAGGAAAGAACTG ATCTTTATGCAAAGCAGGGTCGAGGAAGCCAGTTTACATCAAAAGAAGAAAGAGATAAGTGGATTAAGAA GGAACTGAAGTCTTTAGATCAGGCTATTAATGATAAGAAAAGACAGATTGCTGCTATACACAAGGATTTG GAGGATACCGAGGCCAATAAAGAGAAAAATCTGGAGCAATATAATAAACTGGATCAGGATCTCAATGAAG TCAAAGCTCGAGTTGAAGAACTGGACAGAAAATATTATGAAGTGAAAAATAAGAAAGATGAACTACAAAG TGAAAGAAATTACTTGTGGAGAGAGGAGAATGCAGAACAGCAAGCACTTGCCGCTAAGAGAGAAGACCTT GAGAAGAAGCAGCAGCTTCTTAGAGCGGCAACAGGAAAGGCCATTTTAAATGGAATCGACAGCATTAACA AAGTGTTAGACCATTTCCGGCGGAAGGGTATAAACCAGCATGTGCAAAATGGCTACCATGGCATCGTGAT GAATAACTTTGAGTGCGAACCAGCTTTCTATACTTGTGTGGAAGTCACTGCCGGTAACAGGTTATTCTAT CACATTGTCGATTCAGATGAAGTCAGCACGAAGATCTTAATGGAGTTCAATAAAATGAATCTTCCTGGAG AGGTGACTTTCCTGCCTCTTAACAAGTTAGATGTGAGGGACACTGCCTATCCTGAAACTAACGATGCTAT TCCTATGATCAGTAAGCTGAGGTACAATCCCAGATTCGACAAAGCTTTCAAACATGTGTTTGGAAAGACA CTCATCTGTCGGAGCATGGAGGTTTCCACTCAGCTAGCTCGTGCCTTCACTATGGACTGCATTACTCTGG AAGGTGATCAAGTCAGCCATCGAGGTGCTCTGACTGGAGGCTATTACGACACAAGAAAGTCTCGCCTTGA GTTACAGAAGGATGTTAGGAAAGCAGAGGAGGAGCTGGGTGAGCTCGAAGCTAAGCTCAATGAAAACCTG CGCAGAAACATTGAAAGGATTAATAATGAAATTGATCAATTGATGAACCAAATGCAGCAGATAGAGACCC AACAGAGAAAATTTAAAGCATCCAGAGATAGCATCTTATCAGAGATGAAGATGCTAAAAGAGAAGAGACA GCAGTCAGAAAAGACCTTCATGCCAAAGCAACGCAGTTTGCAGAGTCTGGAGGCCAGTCTGCACGCTATG GAGTCGACCAGAGAGTCGCTGAAGGCAGAGCTAGGGACAGATTTGCTCTCTCAGCTCAGCCTGGAAGACC AGAAAAGAGTCGACGCACTGAATGACGAGATCCGGCAGCTTCAGCAGGAAAACAGACAGCTGCTGAATGA AAGAATTAAACTAGAAGGCATTATTACTCGAGTAGAGACTTACCTCAATGAGAATCTGAGGAAACGTCTG GACCAAGTAGAGCAGGAACTTAATGAACTGAGAGAGACAGAAGGTGGCACTGTTCTTACTGCCACCACGT CAGAACTTGAAGCTATTAATAAAAGAGTGAAAGATACTATGGCAAGATCAGAAGATTTGGATAATTCCAT TGACAAAACAGAAGCTGGAATTAAAGAGCTCCAGAAAAGCATGGAGCGCTGGAAGAACATGGAGAAGGAG CACATGGATGCCATAAATCACGACACTAAAGAGCTGGAGAAGATGACCAACCGACAGGGCATGCTGTTGA AGAAGAAAGAGGAGTGCATGAAGAAGATCCGAGAGCTGGGGTCCCTTCCCCAGGAAGCATTTGAAAAGTA CCAGACACTGAGCCTGAAGCAGTTGTTTCGGAAACTTGAGCAGTGCAACACAGAGTTGAAGAAGTACAGC CATGTCAACAAGAAGGCTCTGGACCAGTTTGTGAATTTCTCTGAGCAGAAGGAGAAACTGATAAAGCGAC AGGAAGAATTGGACAGGGGCTACAAGTCAATCATGGAGTTGATGAATGTACTCGAACTTAGAAAATATGA AGCTATCCAGTTAACCTTCAAACAGGTATCTAAGAACTTTAGTGAAGTTTTCCAGAAGTTGGTACCTGGG GGAAAAGCGACCTTGGTGATGAAGAAAGGAGACGTGGAGGGCAGCCAGTCTCAGGACGAAGGAGAGGGGA GCGGCGAGAGCGAGAGGGGCTCTGGGTCACAAAGCAGCGTTCCATCAGTTGACCAGTTCACAGGAGTCGG GATCAGGGTATCATTTACAGGAAAGCAAGGCGAGATGAGAGAAATGCAGCAGCTTTCAGGAGGGCAGAAG TCTCTGGTAGCCCTTGCTCTCATTTTTGCCATTCAGAAGTGTGACCCTGCTCCTTTCTACCTGTTTGATG AGATCGACCAAGCTTTGGATGCTCAGCACAGAAAAGCTGTGTCAGATATGATTATGGAACTAGCTGTACA CGCCCAGTTTATCACTACCACTTTTAGGCCTGAACTGCTTGAGTCTGCTGATAAATTCTACGGTGTAAAG TTCAGAAATAAGGTTAGTCACATCGACGTGATCACAGCAGAGATGGCCAAAGACTTTGTAGAAGACGATA CCACGCATGGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031583
Insert Size	3654 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031583.2, NP_113771.2
RefSeq Size	4158 bp
RefSeq ORF	3654 bp
Locus ID	29486
Gene Summary	core protein of a chondroitin sulfate proteoglycan that is a component of basement membranes [RGD, Feb 2006]

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