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Polr2e (NM_001109614) Rat Untagged Clone
CAT＃: RN213420
Polr2e (untagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (Polr2e), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213420 representing NM_001109614
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACGAGGAGGAAACGTATCGGCTGTGGAAGATCCGCAAGACTATCATGCAGCTGTGTCATGACC GAGGCTACCTGGTGACCCAGGATGAGCTGGACCAGACACTTGAAGAGTTCAAGGCACAGTTTGGGGACAA GCCCAGCGAAGGACGGCCGAGGCGCACAGACCTCACCGTGCTGGTGGCCCACAACGATGATCCCACAGAC CAGATGTTTGTCTTCTTCCCAGAGGAGCCCAAGGTGGGCATCAAGACCATCAAGGTGTACTGCCAGCGCA TGCAGGAGGAGAACATCACACGGGCGCTGATCGTGGTGCAGCAGGGCATGACACCCTCCGCCAAGCAGTC CCTGGTAGACATGGCCCCTAAGTACGTGCTAGAACAGTTTCTACAGCAGGAGCTGCTCATCAATATCACA GAACATGAGCTAGTCCCTGAGCACGTGGTCATGACCAAGGAGGAGGTGACGGAACTGCTGGCGCGATACA AGCTCCGTGAGAGCCAGCTGCCCAGGATCCAGGCTGGGGACCCGGTGGCACGGTACTTCGGGATCAAGCG AGGGCAGGTTGTGAAGATTATCCGCCCCAGTGAGACAGCCGGCCGGTACATCACCTACCGCCTGGTGCAG TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001109614 |
| Insert Size | 633 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001109614.1, NP_001103084.1 |
| RefSeq Size | 1082 bp |
| RefSeq ORF | 633 bp |
| Locus ID | 690966 |
| UniProt ID | B0BNE2 |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213420 | Polr2e (Myc-DDK-tagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (Polr2e), (10 ug) |
CNY 3,990.00 |
|
| RR213420L3 | Lenti ORF clone of Polr2e (Myc-DDK-tagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (Polr2e), (10 ug) |
CNY 6,080.00 |
|
| RR213420L4 | Lenti ORF clone of Polr2e (mGFP-tagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (Polr2e), (10 ug) |
CNY 6,650.00 |
