Pomk (NM_001024883) Rat Untagged Clone
CAT#: RN213415
Sgk196 (untagged ORF) - Rat similar to RIKEN cDNA 4930444A02 (RGD1310810), (10 ug)
CNY 3,990.00
Product images
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | RGD1310810; Sgk196 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN213415 representing NM_001024883
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACAGCAACATGGAGCCAGGAATGGTCTTACCCACAGAGAGCTCCCCCGGGGCATGGGGCTACTGC TCGCCATGGCCCTTATGAACGTGGTCCTCTACGTCTGCCTCGATCATCTCTTCATCTCCCCTGGGCGAGC TACTGAGGACCCTCGGCGCTGCCCTCCGGGTTACTTCAGAATGGGGCGGATGAGAAACTGCTCGCGCTGG TTGTCCTGTGAGGAGCTGAGGACGGAAGTCAGGCAGCTGAAGCTCGTTGGGGAAGGAGCCGTGAAGAGAG TCTTTCTGTCTGAATGGAATGAACACAAAGTCGCTCTCTCCCGGCTCACCAGGCTGGAAATGAAGGAGGA CTTCCTACATGGACTGCGGATGCTGACATCGCTACAGAGCCAGCACGTGGTCACACTGGCCGGCTTCTGT GAGGAAGACGGCACGATTCTCACCGAGTACCACCCCTTAGGTTCCCTGAGCAACCTGGAAGAAACACTAA ACCTTTCAAAGTACCGAGACGTGAACACTTGGCAGCACAGGCTGAGGCTAGCCGTGGAGTACGTCAGCAT CATTAACTACCTGCACCACAGCCCCCTGGGCACGCGGGTCATGTGTGACTCTAACGACCTGCCCAAAACG TTGTCCCAGTACCTGCTGACAAGTAACTTCAGCATTGTGGCTAACGACCTGGACGCGCTGCCCCTGGTGG ACCACGGCTCTAGGGTACTTGTGAAGTGTGGCCACAGGGAGCTCCACGGGGATTTCGTGGCCCCAGAGCA GCTGTGGCCCTACGGAGAAGACACACCCTTCCAAGATGACCTCATGCCCCCCTATGATGAGAAGATTGAC ATCTGGAAGATTCCGGACGTCTCCAGTTTCCTTCTGGGGCACGTGGAAGGGAGTGACATGGTTAGATTCC ATTTGTTTGATATCCATAAGGCGTGTAAGAACCAGTTCCCCGCAGAGAGACCCACCGCTCAGAACGTTCT AGACGCCTACCAGAAGGTTTTCCACTCCCTCCGAGATACCGTGATGTCGCAGACGAAAGAAATGCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001024883 |
Insert Size | 1050 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024883.1, NP_001020054.1 |
RefSeq Size | 2967 bp |
RefSeq ORF | 1050 bp |
Locus ID | 306549 |
UniProt ID | Q4V8A9 |
Gene Summary | Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR213415 | Sgk196 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 4930444A02 (RGD1310810), (10 ug) |
CNY 3,990.00 |
|
RR213415L3 | Lenti ORF clone of Sgk196 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 4930444A02 (RGD1310810), (10 ug) |
CNY 6,080.00 |
|
RR213415L4 | Lenti ORF clone of Sgk196 (mGFP-tagged ORF) - Rat similar to RIKEN cDNA 4930444A02 (RGD1310810), (10 ug) |
CNY 6,650.00 |