Mapk8ip3 (NM_001100673) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	JIP3; JSAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213400 representing NM_001100673 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAGATCCAGATGGACGAGGGAGGCGGTGTGGTGGTGTACCAAGATGACTACTGCTCCGGCTCGG TGATGTCGGAGCGTGTGTCGGGCCTGGCGGGCTCCATCTACCGCGAGTTCGAGCGCCTCATTCACTGCTA TGATGAGGAGGTGGTCAAGGAGCTCATGCCGCTGGTGGTGAACGTGCTGGAGAACCTTGACTCGGTGCTG AGCGAGAACCAGGAGCACGAGGTGGAGCTGGAGCTCCTACGCGAGGACAACGAGCAGCTGCTCACGCAAT ACGAGCGCGAGAAGGCGCTGCGCAAACAGGCCGAGGAGAAATTCATCGAATTTGAAGATGCCTTGGAACA AGAGAAGAAAGAACTCCAGATCCAGGTAGAACATTATGAGTTTCAGACACGCCAGCTGGAGCTAAAGGCC AAAAACTATGCAGATCAGATTTCCCGACTGGAGGAGCGGGAGTCGGAGATGAAGAAGGAATACAACGCGC TGCACCAGCGGCACACGGAGATGATCCAGACCTATGTGGAACACATCGAAAGATCGAAGATGCAGCAGGT TGGGGGAGGCGGCCAAACAGAAAGCAGCCTGCCCGGGCGGAGCAGGAAGGAGCGTCCCACCTCTCTGAAT GTCTTCCCCCTGGCTGATGGAATGGTACGTGCACAGATGGGGGGCAAGCTCGTGCCTGCGGGGGACCACT GGCACCTGAGTGACCTCGGCCAACTACAGTCCAGCTCCAGCTACCAGTGTCCAAACGATGAGATGTCTGA GTCAGGCCAGTCCTCAGCAGCTGCAACACCCAGTACCACAGGTACCAAATCCAACACACCCACATCCTCC GTGCCCTCAGCAGCTGTCACACCACTCAACGAGAGCCTACAGCCCCTGGGGGACTATGGCAGTGTCACAA AGAACAACAAACGGGCCCGAGAGAAGCGCAACAGCCGTAACATGGAGGTCCAGGTCACCCAAGAGATGCG GAACGTCAGTATTGGTATGGGTAGCAGTGACGAGTGGTCCGATGTTCAGGACATTATCGACTCCACCCCA GAGCTGGATGTGTGTCCGGAAACCCGTCTGGACCGCACAGGAAGCAGCCCAACCCAGGGGATCGTAAACA AAGCTTTTGGAATCAACACCGATTCCCTGTACCATGAACTATCTACAGCGGGGTCTGAGGTCATCGGGGA TGTGGACGAGGGAGCTGATCTTCTAGGGGAGTTCTCAGGAATGGGCAAAGAAGTGGGGAACCTGCTGCTG GAGAACTCACAGCTTCTAGAGACAAAAAATGCTTTGAATGTGGTGAAGAATGACCTCGTTGCCAAGGTTG ACCAACTGTCAGGAGAGCAGGAGGTACTGAAGGGTGAATTGGAAGCAGCCAAGCAAGCCAAAGTCAAGCT GGAGAACCGAATCAAAGAGCTTGAAGAAGAACTAAAAAGAGTCAAGTCAGAGGCAGTAACCGCCCGCCGT GAGCCCAGAGAAGAGGTGGAGGATGACAAAATCCCCATGGCCCAACGCCGACGCTTCACAAGAGTGGAGA TGGCCCGAGTGCTCATGGAACGTAACCAGTACAAGGAACGCCTCATGGAGCTGCAGGAGGCTGTGCGGTG GACTGAAATGATCAGAGCATCCAGGGAACACCCATCTGTCCAGGAAAAGAAGAAATCCACTATCTGGCAG TTCTTTAGCCGCCTCTTCAGCTCCTCATCCAGCCCCCCTCCGGCCAAACGGTCCTACCCGTCTGTGAACA TTCACTACAAGTCACCCACTACAGCTGGCTTTAGCCAGCGTCGCAACCATGCTTTGTGCCAGATCTCAGC CGGCAGCCGACCCCTGGAGTTCTTCCCTGATGATGACTGCACCTCTTCTGCCCGGCGTGAGCAGAAGCGG GAGCAGTACCGCCAGGTCCGTGAACACGTGCGCAATGATGACGGGAGGCTGCAGGCCTGTGGGTGGAGCC TGCCTGCCAAGTACAAGCAGCTGAGCCCCAATGGAGGCCAGGAAGATACCCGGATGAAAAATGTGCCTGT CCCTGTGTACTGCCGCCCTCTGGTGGAGAAGGACCCCTCAACAAAGCTGTGGTGTGCTGCTGGTGTCAAC CTGAGTGGGTGGAAACCAAACGAGGAGGACTCTAGCAATGGACCCAAGCCTGCACCAGGTCGAGACCCTC TGACCTGCGACCGGGAAGGAGAAGGCGAACCCAAGAGCACCCACCCATCACCTGAGAAGAAGAAGGCAAA GGAGGTCCCTGAGGCGGATGCTACCTCCAGTCGGGTATGGATCCTCACCAGCACCCTGACTACCAGCAAG GTGGTGATCATTGATGCCAACCAACCAGGCACAGTTGTGGATCAGTTCACAGTCTGCAATGCCCACGTCC TGTGTATCTCCAGCATTCCTGCGGCCAGCGACAGTGATTATCCCCCTGGAGACATGTTCCTAGACAGTGA TGTGAACCCCGAAGACTCAGGTGCTGACGGTGTGCTGGCTGGCATCACCCTGGTGGGATGTGCTACCCGC TGCAATGTACCACGTAGCAACTGTTCCTCCCGAGGAGACACCCCAGTACTGGACAAGGGGCAGGGGGATG TGGCAGCCACTGCCAATGGGAAGGTCAACCCATCCCAATCCACAGAAGAAGCCACAGAAGCCACGGAGGT GCCAGATCCTGGTCCCAGTGAGTCAGAAGCAACAACAGTCCGGCCTGGGCCTCTCACAGAGCATGTCTTT ACTGACCCAGCACCCACCCAATCCTCCAGCACCCAGCCTGCCAGTGAGAATGGGTCAGAGTCGGATGGCA GCATTGTACAGCCTCAGATGGAGCCCAGTGGGGAGTCCTCAGCAACCACCAGTAGTGCTGCACCCACCAT GTGGCTAGGAGCCCAGAATGGCTGGCTCTATGTGCACTCAGCGGTAGCCAACTGGAAGAAGTGTCTGCAC TCCATCAAACTAAAAGACTCTGTGCTGAGCCTGGTGCATGTCAAAGGCCGAGTGCTGGTAGCTCTTGCAG ATGGAACCCTGGCTATCTTCCATCGTGGAGAGGATGGCCAGTGGGACCTGAGCAACTACCACCTAATGGA CCTGGGCCACCCACACCACTCCATCCGCTGCATGGCTGTTGTGGATGACCGAGTTTGGTGTGGCTACAAG AACAAGGTGCATGTCATCCAGCCCAAGACAATGCAAATTGAGAAATCATTTGATGCCCACCCACGGCGGG AAAGCCAGGTACGACAGCTAGCCTGGATCGGTGATGGAGTATGGGTCTCTATTCGCTTGGATTCTACACT TCGGCTGTACCACGCTCACACGCACCAGCACCTGCAGGATGTGGACATTGAGCCCTATGTTAGCAAGATG CTGGGTACCGGCAAGCTGGGCTTCTCCTTCGTGCGCATCACAGCCCTACTCATTGCAGGCAACCGTCTGT GGGTGGGCACTGGCAATGGGGTTGTCATCTCCATCCCCTTGACTGAGACTGTGGTCCTGCATCGAGGCCA GCTCTTAGGTCTCCGAGCCAACAAGACATCCCCAACGTCTGGGGAGGGGACCCGCCCAGGGGGCATCATC CATGTGTATGGGGATGACAGCAGTGACAAGACTGCCAGTAGTTTCATCCCCAACTGCTCCATGGCCCAGG CTCAGCTCTGCTTCCACGGGCACCGTGATGCTGTCAAATTCTTTGTCTCTGTACCAGGAAATGTGCTGGC CACGCTCAATGGCAGTGTGCTAGACAGCCCATCAGAGGGCCCTGGGCCTGCTGCACCTGCTGCAGATGCT GAGGGCCAGAAGTTGAAGAATGCACTGGTGCTGAGTGGTGGTGAAGGTTACATTGACTTCCGCATCGGGG ATGGAGAGGATGATGAAACTGAGGAAGGTACCGGGGATGTGAACCAGACGAAGCCCTCGTTGTCCAAGGC TGAGCGCAGCCACATCATCGTGTGGCAGGTGTCCTACACCCCTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100673
Insert Size	3969 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100673.1, NP_001094143.1
RefSeq Size	3969 bp
RefSeq ORF	3969 bp
Locus ID	302983
UniProt ID	E9PSK7
Gene Summary	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner. Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation. Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration. Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development. Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation.[UniProtKB/Swiss-Prot Function]

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