Adgrl3 (NM_130822) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cirl3; Lec3; Lphn3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213181 representing NM_130822 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTCCACCTCAGCTGTTCATCCTCATGATGCTTTTAGCACCTGTAGTTCATGGTGGCAAGCACAATG AGAGACATCCAGCCCTCGCTGCTCCACTGCGACATGCTGAGCACAGCCCAGGAGGCCCTCTCCCTCCCAG ACATCTTCTTCAGCAGCCAGCTGCAGAGCGCTCTACAGCTCATCGAGGACAAGGGCCACGTGGAACTGCC AGAGGAGTTCGCGGACCCGGTGCCCCAGGAGCACAGATTGCAGCCCAAGCTTTCAGCCGTGCCCCAATTC CCATGGCAGTGGTCCGCAGAGAGCTCTCCTGTGAGAGCTACCCCATTGAGCTACGCTGTCCAGGCACAGA CGTCATCATGATCGAAAGCGCCAACTACGGGAGGACAGATGACAAGATCTGTGACTCGGACCCTGCTCAG ATGGAGAATATTCGGTGTTATCTGCCAGATGCCTATAAGATTATGTCTCAAAGATGCAATAACAGAACCC AGTGTGCAGTGGTGGCAGGTCCTGATGTATTTCCAGACCCATGTCCGGGAACATATAAATACCTTGAAGT GCAGTATGAATGTGTCCCTTACAAAGTGGAACAAAAAGTTTTTCTTTGTCCCGGACTGCTCAAAGGAGTG TACCAGAGCGAACACTTGTTTGAATCTGACCACCAATCTGGGGCATGGTGCAAAGACCCTCTACAGGCTT CTGACAAGATTTACTATATGCCCTGGACTCCCTACAGAACCGATACCCTGACAGAGTATTCATCCAAAGA TGACTTCATTGCTGGAAGGCCGACAACTACATACAAGCTCCCTCACAGAGTGGATGGTACTGGATTTGTA GTATATGATGGTGCCCTGTTCTTCAACAAGGAGCGTACAAGGAACATAGTAAAGTTTGATTTGAGGACTA GGATAAAGAGTGGAGAGGCAATCATAGCAAATGCTAACTACCATGATACCTCCCCATACCGATGGGGTGG CAAGTCCGACATAGACTTGGCAGTGGATGAAAACGGATTATGGGTAATCTATGCAACAGAACAGAACAAT GGCAAAATTGTTATTAGCCAGTTGAACCCTTACACCCTACGGATTGAGGGGACATGGGACACTGCCTATG ATAAAAGGTCTGCTTCCAATGCATTTATGATTTGTGGGATTCTGTATGTGGTCAAGTCTGTATATGAGGA TGACGACAATGAGGCCACCGGTAATAAGATTGACTACATTTACAATACTGACCAAAGCAAGGATAGCCTG GTGGATGTACCCTTTCCCAACTCATACCAGTACATAGCAGCCGTGGACTACAATCCCAGGGACAATCTGC TGTACGTGTGGAACAACTACCATGTTGTCAAATACTCCTTGGACTTCGGGCCTCTGGATAGCAGATCAGG GCCAGTGCATCATGGACAAGTTTCCTACATCTCTCCACCGATTCACCTTGACTCTGACCTGGAAAGGCCC CCTGTCAGAGGGATTTCTACCACAGGACCCCTGGGTATGGGAAGCACGACCACCAGCACCACCCTCCGGA CTACCACCTGGAACCTAGGGAGGAGTACAACGCCATCCTTGCCTGGCAGAAGAAACCGCAGTACCAGTAC GCCGTCCCCAGCGATTGAGGTGCTGGATGTTACCACACACCTGCCATCTGCAGCCTCCCAAATCCCAGCG ATGGAAGAGAGCTGCGAGGCTGTGGAAGCCCGAGAGATCATGTGGTTTAAGACCCGACAGGGGCAAGTAG CAAAGCAGTCATGCCCAGCAGGAACCATAGGTGTATCAACTTACCTGTGTCTTGCTCCTGATGGAATATG GGATCCCCAAGGACCAGATCTCAGCAACTGCTCTTCTCCTTGGGTCAATCACATAACACAGAAGCTGAAA TCTGGAGAAACAGCTGCCAATATTGCCAGAGAGCTAGCAGAACAGACCAGAAATCATTTGAACGCCGGGG ATATCACCTACTCAGTTCGTGCCATGGACCAACTGGTTGGCCTCCTGGACGTACAGCTCAGGAATTTGAC ACCAGGGGGGAAGGACAGTGCTGCCCGAAGCTTGAACAAGCTTCAGAAAAGAGAGCGCTCTTGCAGAGCC TATGTCCAGGCGATGGTGGAGACAGTTAACAATCTCCTTCAGCCACAAGCTCTGAATGCGTGGAGGGACC TGACGACAAGTGATCAACTACGCGCAGCCACCATGTTGCTCGACACTGTGGAGGAGAGTGCTTTCGTGTT AGCCGATAACCTTTTGAAGACCGACATTGTCAGGGAGAATACAGACAATATTCAGTTGGAGGTTGCAAGG CTGAGCACGGAAGGAAACCTAGAAGATCTAAAATTTCCAGAAAACACGGGCCACGGAAGCACTATACAGC TTTCCGCAAACACGTTAAAGCAAAATGGCCGGAATGGAGAGATTAGAGTGGCCTTTGTCCTGTATAACAA CCTGGGTCCTTATTTATCTACGGAGAATGCCAGTATGAAGTTGGGCACAGAAGCTATGTCCACAAATCAC TCAGTTATCGTCAATTCCCCTGTTATTACAGCAGCAATAAATAAGGAATTCAGTAATAAAGTGTATTTGG CTGATCCTGTGGTATTTACTGTTAAACATATCAAGCAGTCAGAGGAAAATTTCAACCCTAACTGTTCATT TTGGAGCTATTCCAAGCGCACAATGACAGGTTATTGGTCAACACAAGGCTGTCGACTCCTGACAACGAAC AAGACACACACTACGTGCTCCTGTAACCACCTCACCAACTTCGCAGTATTAATGGCACATGTGGAAGTTA AGCACAGCGATGCCGTCCACGATCTTCTTCTGGATGTGATCACGTGGGTCGGAATCCTGTTGTCTCTTGT TTGTCTCCTGATCTGCATCTTCACATTCTGCTTCTTCCGTGGGCTTCAGAGCGACCGTAACACCATTCAC AAGAACCTGTGCATCAGCCTGTTTGTGGCAGAACTGCTCTTCCTGATTGGGATCAACAGAACCGACCAAC CGATTGCCTGTGCAGTGTTTGCGGCTCTTTTGCATTTCTTCTTCTTGGCGGCCTTCACCTGGATGTTTCT AGAAGGGGTACAGCTGTATATCATGCTGGTGGAGGTCTTTGAGAGTGAGCATTCCCGTAGGAAGTACTTC TATCTGGTTGGCTACGGGATGCCCGCACTCATCGTGGCCGTTTCTGCTGCAGTCGACTACAGGAGCTATG GAACAGACAAAGTATGTTGGCTTCGCCTTGACACCTACTTCATTTGGAGTTTTATAGGACCAGCGACCTT GATAATTATGCTGAATGTAATCTTCCTCGGGATTGCTTTATACAAAATGTTTCACCATACTGCCATACTG AAACCTGAATCAGGCTGTCTTGATAATATCAACTATGAGGATAACAGACCCTTCATCAAGTCATGGGTTA TAGGTGCAATAGCGCTGCTCTGCCTATTAGGATTGACCTGGGCCTTTGGACTCATGTATATTAATGAAAG CACAGTCATCATGGCGTATCTCTTCACCATTTTCAATTCTCTACAGGGGATGTTTATATTCATTTTCCAC TGTGTCCTACAGAAGAAGGTACGGAAAGAGTATGGGAAATGCCTACGGACGCATTGCTGTAGTGGGAAAA GCACGGAGAGTTCGATTGGCTCAGGGAAAACATCTGGTTCTCGAACTCCAGGACGGTATTCCACAGGCTC ACAGAGCCGGATTCGGAGAATGTGGAATGACACCGTCCGAAAGCAGTCAGAGTCATCCTTCATCACTGGA GACATAAACAGCTCAGCGTCGCTCAACAGAGGATCCTATCTTCCCTGCATTCAGGCGTGTGTAACATATT TAGAGGGGCTTCTGAACAATGCCAGGGATACAAGTGTCATGGATACTCTACCACTGAATGGTAACCATGG CAACAGTTACAGCATTGCTGGCGGCGAATACCTGAGCAACTGTGTGCAAATTATAGACCGTGGCTATAAC CACAACGAGACCGCCCTAGAAAAAAAGATCCTAAAGGAACTCACTTCCAACTATATCCCTTCATACCTGA ACAACCACGAGCGCTCCAGCGAACAGAACCGGAACATGATGAACAAACTGGTGGACAACTTAGGCAGTGG GAGTGAAGATGACGCCATAGTCCTGGATGACGCAGCGTCCTTTAACCACGAGGAGAGTCTGGGCCTGGAA CTCATTCACGAGGAATCGGATGCTCCCTTGCTGCCCCCGAGGGTTTACTCCACCGATAACCACCAGCCAC ACCATTACAGCAGGAGGCGGCTCCCCCAGGACCACAGCGAGAGCTTCTTCCCTCTGCTAACCGACGAGCA CACAGAAGACCCGCAGTCACCGCACAGGGACTCTCTGTACACCAGCATGCCGGCCCTGGCCGGTGTGCCC GCTGCAGACAGTGTGACCACCAGCACCCAGACCGAAGCCGCAGCGGCCAAGGGTGGTGACGCCGAAGATG TTTACTACAAAAGCATGCCAAACCTGGGCTCCAGAAACCATGTGCACCCGCTGCACGCCTACTACCAGCT AGGGCGAGGCAGCAGCGATGGATTCATAGTTCCTCCCAATAAAGATGGGGCCTCTCCGGAGGGGACTTCC AAAGGACCGGCGCACTTGGTCACTAGTCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_130822
Insert Size	4653 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_130822.1, NP_570835.1
RefSeq Size	5413 bp
RefSeq ORF	4653 bp
Locus ID	170641
UniProt ID	Q9Z173
Gene Summary	G-protein-coupled receptor [RGD, Feb 2006]

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