Brca1 (NM_012514) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212756 representing NM_012514 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTATCTGCTGTTCGAATTCAAGAAGTACAAAATGTCCTTCATGCTATGCAGAAAATCTTGGAGT GTCCAATCTGTTTGGAACTGATCAAAGAACCGGTTTCCACACAGTGCGACCACATATTTTGCAAATTTTG TATGCTGAAACTCCTTAACCAGAAGAAAGGACCTTCCCAGTGTCCTTTGTGTAAGAATGAGATAACCAAA AGGAGCCTACAAGGAAGTGCAAGGTTTAGTCAACTTGTTGAAGAGCTGCTGAAAATCATTGATGCTTTTG AGCTTGACACCGGAATGCAGTGTGCAAATGGTTTTAGTTTTTCAAAAAAGAAAAATAGTTCTTCTGAGCT TCTGAATGAGGACGCTTCTATCATCCAGAGTGTGGGCTACCGAAACCGCGTCAAAAAACTTCAACAGATT GAGTCTGGAAGTGCCACCTTGAAGGACAGTCTCAGTGTCCAGTTGTCTAACCTTGGGATTGTGAGATCAA TGAAGAAAAATAGGCAGACACAACCTCAAAACAAATCTGTCTACATTGCTTTAGAATCTGATTCTTCTGA GGAGAGAGTAAATGCACCCGATGGCTGCAGTGTGAGAGATCAGGAATTGTTTCAGATCGCCCCTGGAGGA GCTGGAGATGAAGGCAAGTTGAACTCTGCAAAAAAGGCTGCTTGTGACTTTTCTGAGGGCATAAGAAACA TTGAACATCATCAATGCAGTGATAAAGATTTGAACCCCACTGAGAATCATGCAACTGAAAGGCATCCAGA AAAATGTCCGCGTATTTCTGTTGCAAACGTGCACGTGGAGCCGTGTGGCACAGATGCTCGTGCCAGCTCA TTACAGCGTGGGACCCGCAGTTTATTGTTCACTGAGGACAGACTGGATGCAGAAAAGGCTGAATTCTGTG ATAGAAGCAAACAGTCTGGCGCAGCAGTGAGCCAGCAGAGCAGATGGGCTGACAGTAAAGAAACATGTAA TGGCAGGCCGGTTCCCCGCACTGAGGGAAAGGCAGATCCAAATGTGGATTCCCTCTGTGGTAGAAAGCAG TGGAATCATCCGAAAAGCCTGTGCCCTGAGAATTCTGGAGCTACCACTGACGTTCCTTGGATAACACTGA ATAGCAGCATTCAGAAAGTGAATGAGTGGTTTTCCAGAACTGGTGAAATGTTAACTTCTGACAATGCATC TGACAGGAGGCCTGCGTCAAATGCAGAAGCTGCTGTTGTGTTAGAAGTTTCAAATGAAGTGGATGGATGT TTCAGTTCTTCAAAGAAAATAGACTTAGTTGCCCCTGATCCCGATAATGCTGTAATGTGTACAAGTGGAA GAGACTTCTCCAAGCCAGTAGAGAATATTATCAACGATAAAATATTTGGGAAAACCTATCAGAGAAAGGG AAGCCGCCCTCACTTGAACCATGTGACTGAAATTATAGGCACATTTACTACAGAACCACAGATTATACAA GAGCAGCCCTTCACAAATAAATTAAAACGCAAAAGAAGTACATGCCTTCATCCTGAGGACTTTATCAAGA AAGCAGATTTAACAGTTGTTCAAAGGATTTCTGAAAATTTAAATCAGGGAACTGACCAAATGGAGCCAAA TGACCAAGCAATGAGTATTACCAGTAACGGTCAGGAGAACAGAGCAACAGGTAATGATCTTCAGAGAGGG AGAAATGCTCATCCAATAGAATCATTGAGAAAGGAACCTGCTTTCACAGCTAAAGCCAAATCTATAAGCA ACAGTATAAGTGATTTGGAGGTAGAATTAAATGTTCACAGTTCAAAAGCACCTAAGAAAAATAGGCTGAG GAGGAAGTCTACCAGGTGTGTTCTTCCACTCGAACCAATCAGTAGAAATCCGAGCCCACCTACTTGTGCT GAACTTCAGATCGAGAGTTGTGGTAGCAGTGAAGAAACAAAGAAAAACAATTCCAACCAAACCCCAGCCG GGCACATTAGAGAGCCTCAACTCATCGAAGACACAGAACCCGCAGCTGATGCCAAGAAGAACGAGCCAAA TGAACACATAAGGAAGAGAAGTGCCAGTGATGCGTTCCCAGAAGAGAAATTAATGAACAAAGCTGGTTTA TTAACTAGCTGTTCAAGTCCTAGAAAGCCTCAAGGACCTGTCAATCCTAGCCCTGAGAGAAAAGGAATAG AGCAACTTGAAATGTGCCAGATGCCTGATAATAACAAAGAACTCGGGGATTTGGTCCTGGGAGGAGAGCC CAGTGGGAAACCTACTGAACCATCTGAGGAGAGCACCAGTGTGTCCTTGGTACCCGACACAGACTACGAC ACCCAGAACAGTGTCTCAATACTGGAAGCGAACACTGTCAGATATGCAAGAACAGGATCAGTTCAGTGTA TGACTCAGTTTGTCGCAAGTGAAAACCCCAAGGAACTTGTCCATGGTTCTAACAATGCTGGAAGTGGCTC GGAGTGCTTCAAGCACCCATTGAGACATGAACTTAACCACAATCAAGAGACAATAGAAATGGAAGACAGT GAACTTGATACTCAGTATTTGCAGAATACATTTCAAGTTTCAAAGCGTCAGTCATTTGCTTTATTTTCAA AACTTAGAAGTCCCCAAAAGGACTGTACTCTGGTAGGTGCCCGCTCTGTGCCCTCAAGGGAACCAAGTCC AAAGGTGACTTCTAGAGGTGAACAAAAAGAACGTCAGGGACAAGAAGAGTCTGAAATCAGTCATGTACAG GCAGTCACAGTCACAGTAGGCTTACCTGTGCCCTGTCAGGAAGGTAAGCCAGGTGCTGTTACAATGTGTG CTGATGTTTCTAGGCTTTGTCCGTCATCTCATTATAGAAGCTGTGAGAATGGACTCAACACCACAGATAA ATCTGGAATTTCACAAAACTCACATTTTAGACAATCAGTTTCTCCCCTCAGGTCATCTATAAAAACTGAC AATAGAAAAACTCTGACAGAGGGNCGATTTGAGAAACATACTGAAAGGGGRATGGGAAATGAGACTGCTG TTCAAAGTACAATACACACAATTAGTCTAAATAACAGAGGAGATGCTTGTCTAGAAGCCAGCTCAGGCAG TGTTATTGAAGTACATTCCACTGGTGAAAACGTCCAGGGGCAACTAGATAGAAACAGAGGGCCTAAGGTA AACACCGTGTCTCTATTAGATAGTACACAGCCTGGTGTCTCTAAGCAGAGTGCTCCTGTAAGTGATAAGT ATCTTGAAATAAAGCAGGAGAGTAAGGCTGTCAGTGCAGACTTCTCTCCATGTCTGTTCTCAGATCATCT TGAAAAACCTATGAGAAGTGATAAGACTTTTCAGGTTTGCTCTGAGACACCTGATGACCTGTTGGATGAT GTTGAAATACAGGAAAATGCTAGCTTCGGTGAAGGTGGCATAACGGAAAAGTCTGCTATTTTTAATGGAA GTGTCCTGAGAAGAGAGTCCAGTAGGAGCCCTAGCCCTGTAACCCATGCATCGAAGTCGCGGAGTCTCCA CAGAGGGTCTAGGAAATTAGAATTCTCAGAAGAGAGCGACTCCACTGAGGATGAAGACCTTCCCTGCTTC CAACACTTGCTGAGCAGAGTAAGCAGCACACCCGAACTTACCAGATGCAGCAGTGTGGTGACACAGCGTG TGCCAGAGAAAGCGAAGGGGACCCAAGCACCCCGGAAGAGTAGCATCAGTGACTGCAATAATGAGGTGAT CTTGGGAGAGGCATCTCAGGAATATCAGTTTAGTGAGGATGCAAAATGCTCTGGCAGCATGTTCTCCTCA CAGCACAGTGCTGCCCTAGGTTCNCCTGCAAATGCACTCTCCCAGGATCCCGATTTTAATCCACCTTCCA AACAAAGGCGTCACCAGGCTGAGAATGAGGAAGCCTTTCTAAGTGATAAGGAATTGATTTCAGATCATGA GGACATGGCAGCTTGCCTGGAAGAGGCTAGCGATCAAGAAGAGGACAGTATAATCCCGGACTCAGTGGCG TCTGGATATGAGAGTGAAGCAAACCTCTCTGAAGACTGCTCACAGAGTGATATTCTAACCACTCAGCAGA GGGCGACCATGAAGGATAACCTGATAAAGCTCCAGCAGGAAATGGCTCAGCTGGAAGCTGTGCTAGAGCA GCATGGGAGCCAGCCGTCTGGCCACCCCCCTTGCCTCCCAGCTGACCCTTGTGCCCTAGAAGACCTGCCA GATCCAGAACAAAACAGATCAGGAACAGCAATTTTAACTTCAAAGAACATTAATGAGAATCCTGTAAGCC AGAATCCAAAGCGCGCCTGTGATGACAAATCCCAACCACAACCTCCGGACGGTCTCCCCAGTGGAGATAA GGAGTCAGGAATGAGAAGGCCTTCCCCTTTTAAATCTCCACTGACCAGCAGTAGGTGCTCTGCACGAGGC CACTCCAGGAGTCTTCAGAATAGAAACTCCACSTCGCAAGAGGAGCTCCTTCAGCCTGCTGRATTGGAGA AGTCATGTGAACCACACAACTTAACAGGGCGGTCTTGCTTGCCAAGGCAAGATCTCGAAGGAACCCCATA CCCGGAATCTGGAATCAGGCTAGTCTCCAGCAGAGACCCTGATTCTGAATCCCCTAAAGTGTCAGCCCTT GTTTGCACTGCACCAGCTTCAACCTCTGCACTGAAAATATCCCAAGGTCAAGTTGCTGGGTCTTGCAGGA GTCCAGCTGCTGGTGGTGCTGATACTGCAGTGGTAGAAATTGTGAGCAAGATAAAGCCGGAAGTGACGTC TCCAAAAGAACGAGCGGAGAGAGATATATCCATGGTGGTGTCAGGCTTGACCCCTAAAGAAGTAATGATT GTCCAAAAGTTTGCTGAAAAATACCGCCTTGCTTTAACTGATGTAATTACTGAGGAAACTACGCATGTAA TTATAAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTGGGCATTGCAGGAGGAAA GTGGATAGTTAGCTATTCTTGGGTGATCAAGTCTATCCAAGAAAGAAAACTTCTGAGTGTGCATGAATTT GAAGTCAAAGGAGACGTTGTGACTGGAAGCAACCACCAAGGTCCAAGGCGATCCAGAGAATCCCAGGAAA AGCTCTTTGAAGGCCTACAGATCTATTGTTGTGAGCCCTTCACCAACATGCCCAAAGATGAGCTGGAGAG GATGCTGCAGCTGTGTGGGGCTTCTGTGGTGAAGGAGCTTCCATTGCTCACCCGTGACACAGGTGCTCAT CCAATTGTGCTCGTGCAGCCAAGTGCCTGGACAGAAGACAACGACTGCCCTGATATTGGGCAGCTGTGCA AGGGACGTCTAGTGATGTGGGACTGGGTGTTGGACAGTATATCCGTCTACCGGTGTCGGGATCTGGATGC CTACCTGGTACAGAATATCACCTGTGGCCGTGATGGTAGCGAGCCACAAGACTCCAATGATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012514
Insert Size	5454 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012514.1, NP_036646.1
RefSeq Size	5607 bp
RefSeq ORF	5454 bp
Locus ID	497672
UniProt ID	O54952
Gene Summary	involved in the DNA damage response and DNA repair [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.