Ndufs1 (NM_001005550) Rat Untagged Clone
CAT#: RN212687
Ndufs1 (untagged ORF) - Rat NADH dehydrogenase (ubiquinone) Fe-S protein 1 (Ndufs1), nuclear gene encoding mitochondrial protein, (10 ug)
CNY 9,310.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | MGC93795 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN212687 representing NM_001005550
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAAGGATACCTGTAAAAAGGGCCTTGATAGGCCTTTCTAAGTCTCCTAAAGGATATGTTCGATCAA CTGGCACAGCAGCAAGTAACTTGATTGAAGTATTTGTTGATGGTCAGTCTGTCATGGTGGAACCAGGAAC CACTGTCCTGCAGGCCTGCGAGAAGGTTGGCATGCAAATCCCTCGATTCTGTTACCATGAAAGGTTGTCT GTAGCTGGAAATTGCAGGATGTGCCTGGTAGAGATTGAGAAAGCTCCAAAGGTTGTTGCTGCTTGTGCTA TGCCTGTTATGAAGGGCTGGAATATCCTGACAAACTCAGAAAAATCTAAGAAAGCCAGAGAAGGTGTGAT GGAGTTTTTATTAGCAAATCACCCATTGGATTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCTACAG GACCAGTCCATGATGTTTGGAAGTGATAGGAGCCGATTTCTAGAGGGGAAGCGTGCTGTGGAGGACAAGA ACATTGGGCCCCTGGTAAAGACCATCATGACTAGATGCATACAGTGTACCCGCTGCATCAGGTTTGCAAG TGAAATTGCAGGAGTAGATGATTTGGGAACAACGGGGAGAGGAAATGACATGCAAGTTGGAACATACATT GAGAAAATGTTCATGTCTGAACTGTCTGGGAACATCATTGATATCTGCCCTGTCGGGGCCCTAACCTCTA AGCCTTATGCCTTTACTGCCCGGCCTTGGGAAACAAGAAAGACAGAGTCCATTGATGTAATGGATGCAGT GGGAAGTAACATTGTGGTTAGCACAAGAACTGGAGAGGTAATGAGGATTTTGCCAAGAATGCATGAAGAT ATTAATGAAGAATGGATCTCTGATAAAACCAGATTTGCCTATGATGGACTGAAACGGCAAAGACTTACCG AACCAATGGTCAGAAATGAAAAGGGGCTTTTAACTTATACCTCCTGGGAAGATGCACTCTCTCGTGTAGC TGGAATGTTACAGAGTTTTGAAGGCAAGGCTGTGGCAGCAATTGCAGGTGGCTTGGTGGATGCTGAAGCC TTGGTAGCTCTGAAAGATTTGCTTAATAAAGTTGACTCTGACACCTTATGCACTGAAGAGATCTTCCCCA ATGAAGGAGCTGGCACAGATTTACGTTCCAATTATCTTCTCAATACCACAATTGCCGGTGTGGAAGAAGC AGATGTTGTTCTTCTAGTTGGTACAAATCCACGTTTTGAGGCACCGCTGTTTAATGCTAGAATCAGAAAG AGCTGGCTGCATAATGACTTAAAAGTGGCCCTAATAGGCAGTCCAGTAGACCTCACTTACAGATACGACC ATCTAGGAGACTCTCCCAAAATTCTGCAAGACATTGCTTCAGGGAATCATGAATTCAGCAAGGTCTTAAA CGCAGCTAAAAAACCAATGGTGGTTTTAGGCAGTTCTGCACTCCAGAGAGATGATGGGGCAGCAATTCTT GCAGCTGTGTCCAGCATTGCACAAAAGATTCGGGTGGCAAGTGGTGCTGCTGCAGAGTGGAAAGTTATGA ATATTCTGCATAGGATTGCAAGCCAGGTAGCTGCTTTGGACCTTGGCTATAAACCTGGGGTAGAAGCAAT TAGGAAGAACCCACCCAAACTGCTGTTTCTTCTGGGAGCAGATGGAGGTTGTATCACCCGGCAGGACTTG CCAAAGGATTGTTTCATTGTTTATCAAGGACATCATGGTGATGTTGGTGCTCCCATAGCTGATGTTATTC TCCCAGGGGCTGCTTACACAGAAAAGTCTGCTACTTACGTCAATACTGAGGGCAGAGCTCAACAAACCAA AGTAGCAGTGACACCTCCTGGCTTGGCAAGAGAAGACTGGAAAATCATAAGAGCTCTCTCTGAGATTGCA GGTATCACTCTCCCATATGATACTCTGGATCAAGTGAGAAACCGTCTCGGAGAAGTCTCTCCTAACCTGG TTCGATATGATGATGTTGAAGAAGCTAATTACTTTCAACAAGCAAGTGAGCTTGCCAAGCTAGTAGACCA GGAATTTCTTGCTGACCCACTGGTTCCACCTCAGCTAACTATAAAAGACTTCTATATGACAGATTCAATT AGCAGAGCCTCACAGACAATGGCCAAGTGTGTCAAAGCCGTCACAGAAGGCGCTCAGGCAGTAGAGGAGC CATCCATATGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001005550 |
Insert Size | 2184 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001005550.1, NP_001005550.1 |
RefSeq Size | 2519 bp |
RefSeq ORF | 2184 bp |
Locus ID | 301458 |
UniProt ID | Q66HF1 |
Gene Summary | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR212687 | Ndufs1 (Myc-DDK-tagged ORF) - Rat NADH dehydrogenase (ubiquinone) Fe-S protein 1 (Ndufs1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 9,310.00 |
|
RR212687L3 | Lenti ORF clone of Ndufs1 (Myc-DDK-tagged ORF) - Rat NADH dehydrogenase (ubiquinone) Fe-S protein 1 (Ndufs1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 11,120.00 |
|
RR212687L4 | Lenti ORF clone of Ndufs1 (mGFP-tagged ORF) - Rat NADH dehydrogenase (ubiquinone) Fe-S protein 1 (Ndufs1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 12,260.00 |