Fasn (NM_017332) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212398 representing NM_017332 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGTGGTGATAGCCGGTATGTCCGGGAAATTGCCCGAGTCAGAGAACCTGCAGGAGTTCTGGG CCAACCTCATTGGCGGTGTGGACATGGTCACAGACGATGACAGGAGGTGGAAGGCTGGGCTCTATGGGTT GCCTAAGCGGTCTGGAAAGCTGAAGGATCTGTCCAAGTTCGACGCCTCCTTTTTTGGGGTCCACCCCAAG CAGGCACACACAATGGACCCGCAGCTCCGGCTGCTGCTGGAAGTCAGCTATGAAGCTATTGTGGACGGAG GTATCAACCCGGCCTCACTCCGAGGAACAAACACTGGTGTCTGGGTGGGTGTGAGTGGTTCCGAGGCGTC GGAGGCCCTGAGCAGAGATCCTGAGACTCTTCTGGGCTACAGCATGGTGGGCTGCCAGAGAGCAATGATG GCCAACCGGCTCTCTTTCTTCTTCGACTTCAAAGGACCCAGCATTGCCCTGGACACAGCCTGCTCCTCTA GCCTACTGGCACTACAGAATGCCTATCAGGCTATCCGCAGTGGGGAGTGCCCTGCTGCCATTGTGGGCGG GATCAACCTGCTGCTAAAGCCTAACACCTCTGTGCAGTTCATGAAGCTAGGCATGCTCAGCCCCGATGGC ACCTGCAGATCCTTTGATGATTCAGGGAACGGGTATTGCCGTGCTGAGGCTGTCGTGGCAGTTCTGCTGA CTAAGAAGTCCTTGGCTCGGCGAGTCTATGCCACTATTCTGAATGCCGGGACGAACACAGATGGCTGCAA GGAGCAAGGCGTGACATTCCCCTCTGGAGAAGCCCAGGAACAACTCATCCGTTCTCTGTATCAGCCGGGC GGTGTGGCCCCCGAGTCTCTTGAATATATTGAAGCCCATGGCACGGGCACCAAGGTGGGGGACCCCCAGG AACTGAACGGCATTACTCGGTCCCTGTGTGCTTTCCGCCAGAGCCCTTTGTTAATTGGCTCCACCAAATC CAACATGGGACACCCTGAGCCTGCCTCGGGGCTTGCAGCCCTGACCAAGGTGCTGTTATCCCTAGAAAAT GGGGTTTGGGCCCCCAACCTGCATTTCCACAACCCCAACCCTGAAATCCCAGCACTTCTTGATGGGCGGC TGCAGGTGGTCGATAGGCCCCTGCCTGTTCGTGGTGGCATCGTGGGCATCAACTCGTTTGGCTTCGGAGG TGCCAATGTTCACGTCATCCTCCAGCCCAACACACAGCAGGCCCCAGCACCTGCCCCACATGCTGCCCTA CCGCATTTGCTGCATGCCAGTGGACGGACCATGGAGGCAGTGCAGGGCCTGCTGGAACAGGGCCGCCAGC ACAGTCAGGACTTGGCCTTTGTGAGCATGCTCAATGACATTGCAGCAACCCCTACAGCAGCCATGCCCTT CAGAGGTTACACTGTGTTAGGTGTTGAGGGCCATGTCCAGGAAGTGCAGCAAGTGCCTGCCAGCCAGCGC CCACTCTGGTTCATCTGCTCAGGGATGGGCACACAGTGGCGTGGAATGGGGCTGAGCCTTATGCGCCTGG ACAGTTTCCGTGAGTCCATCCTGCGCTCTGATGAGGCTCTGAAGCCCTTGGGAGTCAAAGTGTCAGACCT GCTGCTGAGCACTGATGAGCACACCTTTGATGACATCGTGCATTCCTTTGTGAGCCTCACCGCCATCCAG ATTGCCCTCATCGACCTGCTGACGTCTATGGGGCTGAAACCTGATGGCATCATTGGGCACTCCTTGGGAG AGGTTGCCTGTGGCTATGCAGATGGCTGTCTCTCCCAGAGAGAGGCTGTGCTTGCAGCCTACTGGAGAGG CCAGTGCATTAAGGATGCCAACCTTCCGGCTGGATCCATGGCAGCTGTTGGTTTGTCCTGGGAAGAATGT AAACAACGCTGCCCTCCTGGTGTGGTGCCTGCCTGCCACAACTCTGAGGACACTGTGACCATCTCTGGAC CTCAGGCTGCAGTGAATGAATTTGTGGAGCAGCTAAAGCAAGAGGGCGTGTTTGCCAAGGAGGTGCGAAC AGGTGGCCTGGCCTTCCACTCCTACTTCATGGAAGGAATTGCCCCCACGCTGCTGCAGGCTCTCAAGAAG GTGATCCGGGAGCCACGGCCACGCTCAGCACGCTGGCTCAGCACCTCTATCCCTGAGGCCCAGTGGCAGA GCAGCCTGGCCCGCACATCTTCTGCTGAGTACAACGTCAACAACCTGGTGAGCCCTGTGCTCTTCCAGGA AGCACTGTGGCACGTCCCCGAGCACGCCGTGGTGCTGGAGATTGCACCCCATGCACTGTTGCAGGCTGTC CTGAAGCGAGGCGTGAAGCCTAGCTGCACCATCATCCCCTTGATGAAGAGGGACCATAAAGATAACTTGG AGTTCTTCCTCACCAACCTCGGCAAGGTGCACCTCACAGGCATCGACATCAACCCTAATGCCTTGTTCCC ACCTGTGGAATTCCCGGTTCCCCGAGGGACTCCTCTCATCTCCCCTCACATCAAGTGGGACCACAGTCAG ACTTGGGATATCCCAGTTGCTGAAGACTTCCCCAACGGTTCCAGCTCCTCCTCAGCTACAGTCTACAACA TTGACGCCAGTTCCGAGTCATCTGACCACTACCTGGTCGACCACTGCATTGACGGCCGTGTCCTCTTCCC TGGCACTGGCTACCTGTACCTGGTGTGGAAGACACTGGCTCGAAGCCTGAGCTTGTCCCTAGAAGAGACC CCTGTGGTGTTTGAGAACGTGACATTTCATCAGGCCACCATCCTGCCCAGGACAGGAACCGTGCCTCTGG AGGTGCGGCTGCTAGAGGCCTCACATGCATTTGAGGTGTCTGACAGTGGCAACCTGATAGTGAGCGGGAA AGTGTACCAGTGGGAAGACCCTGACTCCAAGTTATTCGACCACCCAGAAGTCCCGATCCCCGCCGAGTCC GAGTCTGTCTCCCGCTTGACGCAGGGAGAAGTATACAAGGAGCTGCGGCTACGTGGCTATGACTATGGCC CTCATTTCCAGGGCGTCTATGAGGCCACCCTCGAAGGTGAGCAAGGCAAGCTGCTCTGGAAAGACAACTG GGTGACCTTCATGGACACAATGCTGCAGATATCCATCCTGGGCTTCAGCAAGCAGAGTCTGCAGCTACCC ACCCGTGTGACTGCCATCTATATTGACCCTGCAACCCACCTGCAGAAGGTGTACATGCTGGAGGGAGACA CTCAAGTGGCTGACGTGACCACGAGCCGCTGTCTGGGCGTGACCGTCTCTGGTGGTGTCTACATTTCGAG ACTACAGACAACAGCAACCTCACGGCGGCAGCAGGAACAGCTGGTCCCCACCCTGGAGAAGTTTGTCTTC ACACCCCATGTGGAGCCTGAGTGCCTGTCTGAGAGTGCTATCCTGCAGAAAGAGCTGCAGCTGTGCAAGG GTCTGGCAAAGGCTCTGCAGACCAAGGCCACCCAGCAAGGGCTGAAGATGACAGTGCCTGGGCTAGAGGA CCTTCCCCAGCATGGACTGCCTCGACTCTTGGCTGCTGCCTGCCAGCTGCAGCTCAACGGGAACCTGCAA CTGGAGTTAGGTGAGGTACTGGCTCGAGAGAGGCTCCTGCTGCCAGAAGACCCTCTGATCAGTGGCCTCC TTAACTCCCAGGCCCTCAAGGCCTGCATAGACACAGCCCTGGAGAACCTGTCTACTCTCAAGATGAAGGT GGTGGAGGTGCTGGCTGGAGAAGGCCACTTGTATTCCCACATCTCAGCACTGCTCAACACCCAGCCTATG CTGCAACTGGAGTATACAGCCACCGACCGGCACCCCCAGGCCCTGAAGGATGTTCAGACCAAGCTGCAGC AGCATGATGTAGCACAGGGCCAGTGGGACCCTTCTGGTCCTGCTCCTACCAACCTGGGTGCTCTTGACCT TGTGGTGTGCAACTGTGCGTTAGCCACCCTGGGGGATCCAGCCCTGGCCCTGGACAACATGGTAGCTGCC CTCAAGGATGGTGGTTTCCTGCTAATGCACACAGTGCTCAAAGGACATGCCCTTGGGGAAACCCTGGCCT GCCTCCCTTCTGAGGTGCAGCCTGGGCCCAGCTTCTTAAGCCAGGAAGAGTGGGAGAGCCTGTTCTCAAG GAAGGCACTGCACCTGGTGGGCCTTAAAAAGTCATTCTACGGTACTGCGCTGTTCCTGTGCCGCCGTCTC AGCCCACAGGACAAGCCCATCTTCCTGCCTGTGGAGGATACTAGTTTCCAGTGGGTGGACTCTCTGAAGA GCATTCTGGCCACATCCTCCTCCCAGCCTGTGTGGCTAACAGCCATGAACTGCCCCACCTCAGGTGTGGT AGGCTTGGTGAACTGTCTCCGAAAAGAGCCGGGTGGACACCGGATTCGGTGTATCCTGCTGTCCAACCTC AGCAGCACATCTCACGTCCCCAAGCTGGACCCTGGCTCTTCAGAGCTACAGAAGGTGCTAGAGAGTGATC TGGTGATGAACGTGTACAGGGACGGTGCCTGGGGTGCCTTCCGTCACTTCCAGTTAGAGCAGGACAAGCC CGAGGAGCAGACAGCACATGCCTTTGTAAACGTCCTTACCCGAGGGGACCTTGCCTCCATCCGCTGGGTC TCTTCTCCCCTGAAACACATGCAGCCGCCCTCGAGCTCAGGAGCACAGCTCTGCACTGTCTACTATGCCT CACTGAACTTCCGAGATATCATGCTGGCCACGGGCAAGCTGTCCCCTGATGCCATTCCAGGTAAATGGGC CAGCCGGGACTGCATGCTTGGCATGGAGTTCTCAGGCCGTGATAAGTGCGGCCGGCGTGTGATGGGGCTG GTACCCGCAGAAGGCCTGGCCACCTCAGTCCTGTTATCACCCGACTTCCTCTGGGATGTACCCTCTAGCT GGACCCTGGAGGAGGCGGCTTCTGTGCCTGTTGTCTACACCACCGCCTACTACTCCTTAGTAGTGCGTGG TCGTATTCAGCACGGGGAAACTGTGCTCATTCACTCGGGCTCCGGTGGTGTGGGCCAAGCGGCCATTTCC ATTGCCCTTAGCCTGGGCTGCCGAGTCTTCACCACTGTGGGCTCCGCTGAGAAGCGAGCTTACCTCCAGG CCAGATTCCCTCAGCTGGATGACACCAGCTTTGCTAACTCTCGAGACACATCGTTTGAGCAGCATGTGTT ACTGCACACAGGTGGCAAAGGGGTGGACCTGGTCCTCAACTCCCTGGCAGAAGAGAAGCTGCAGGCCAGT GTGCGGTGCTTGGCTCAGCATGGCCGCTTCCTAGAGATCGGCAAATTTGATCTTTCTAACAACCACCCTC TGGGCATGGCCATCTTCTTGAAGAACGTCACTTTCCATGGGATCCTGCTGGATGCACTTTTTGAGGGGGC CAACGACAGCTGGCGGGAGGTGGCAGAGCTGCTGAAGGCCGGCATCCGTGATGGGGTTGTGAAGCCTCTC AAGTGTACAGTGTTTCCCAAGGCCCAGGTGGAGGACGCCTTCCGATACATGGCTCAAGGAAAACATATTG GCAAAGTCCTTGTCCAGGTACGGGAGGAGGAGCCCGAGGCTATGCTGCCAGGGGCTCAGCCCACCCTGAT TTCCGCCATCTCCAAGACCTTCTGCCCAGAGCATAAGAGTTACATCATCACTGGTGGCCTAGGTGGCTTT GGCCTGGAACTGGCCCGGTGGCTTGTGCTTCGTGGGGCCCAAAGGCTTGTACTAACTTCCCGATCTGGAA TCCGCACAGGCTACCAAGCCAAGCACGTTCGGGAGTGGAGGCGCCAGGGCATCCATGTGCTAGTGTCGAC AAGCAATGTCAGTTCACTGGAGGGGGCCCGTGCTCTCATCGCTGAAGCCACAAAGCTTGGGCCCGTTGGA GGTGTCTTCAACCTGGCCATGGTTTTAAGGGATGCCATGCTGGAGAACCAGACTCCAGAACTCTTCCAGG ATGTCAACAAGCCCAAGTACAATGGCACCCTGAACCTTGACAGGGCGACCCGGGAAGCCTGTCCTGAGCT GGACTACTTTGTGGCCTTCTCCTCTGTAAGCTGCGGGCGTGGTAATGCTGGCCAATCCAACTATGGCTTC GCCAACTCTACCATGGAGCGTATTTGCGAACAGCGCCGGCACGATGGCCTCCCAGGTCTTGCCGTGCAAT GGGGTGCCATTGGTGACGTGGGCATTATCTTGGAAGCGATGGGTACCAATGACACAGTCGTTGGCGGCAC ACTGCCACAGCGCATCTCCTCCTGCATGGAGGTGCTGGACCTCTTCCTGAATCAGCCCCACGCAGTCCTG AGCAGTTTTGTGCTGGTTGAGAAGAAAGCTGTGGCCCATGGTGATGGTGAAGCCCAGAGGGATCTGGTGA AAGCAGTGGCACACATCCTAGGCATCCGCGACCTCGCAGGGATTAACCTGGACAGCTCGCTGGCAGACCT CGGCCTGGACTCGCTCATGGGTGTGGAAGTGCGCCAGATCCTGGAACGTGAACATGATCTGGTGCTACCC ATTCGTGAAGTACGGCAACTCACACTGCGGAAGCTTCAGGAAATGTCCTCCAAGGCTGGCTCAGACACTG AGTTGGCAGCCCCCAAGTCCAAGAATGATACATCCCTGAAGCAGGCCCAGCTGAATCTGAGTATCCTGCT GGTGAACCCTGAGGGCCCTACCTTAACACGACTCAACTCAGTGCAGAGCTCTGAGCGGCCTCTGTTCCTG GTGCACCCCATTGAAGGTTCCATCACTGTGTTCCACAGCCTGGCTGCCAAGCTCAGTGTGCCCACCTACG GTCTGCAGTGCACCCAAGCGGCCCCCCTGGACAGCATTCCAAACCTGGCTGCCTACTACATTGATTGCAT CAAGCAGGTGCAGCCTGAGGGGCCCCACCGAGTGGCTGGGTATTCTTTTGGAGCTTGTGTAGCCTTCGAG ATGTGCTCCCAGCTGCAGGCCCAGCAGGGCCCAGCCCCCGCCCACAACAACCTCTTCTTGTTTGATGGCT CACACACCTACGTATTGGCGTACACCCAGAGCTACCGGGCAAAGCTGACCCCAGGCTGTGAGGCTGAGGC TGAAGCTGAAGCCATATGCTTCTTCATTAAGCAGTTTGTTGATGCAGAGCATAGCAAGGTGCTAGAGGCC CTGCTACCACTGAAGAGCCTGGAGGACCGGGTTGCTGCTGCTGTGGACCTCATCACTAGAAGCCACCAGA GCCTGGACCGCCGTGACCTGAGCTTTGCTGCCGTGTCCTTCTACTACAAGCTTCGAGCCGCCGACCAGTA TAAACCCAAGGCCAAGTACCACGGCAATGTGATCCTGCTGCGGGCCAAGACAGGTGGCACCTACGGCGAG GACTTGGGTGCCGATTACAACCTGTCCCAGGTGTGTGATGGGAAGGTGTCTGTGCACATCATTGAGGGTG ACCATCGTACGCTGCTGGAGGGCAGGGGCCTGGAGTCTATCATCAACATCATCCACAGCTCCCTGGCTGA GCCTCGAGTGAGTGTACGGGAGGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017332
Insert Size	7518 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017332.1, NP_059028.1
RefSeq Size	9136 bp
RefSeq ORF	7518 bp
Locus ID	50671
UniProt ID	P12785
Gene Summary	plays a role in lipid biosynthesis [RGD, Feb 2006]

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