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Plekhm1 (NM_001009677) Rat Untagged Clone
CAT＃: RN212281
Plekhm1 (untagged ORF) - Rat pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), (10 ug)
CNY 17,010.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC94075 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN212281 representing NM_001009677
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTCAGTGGAGAATGGCCTGGACCCTCGGGCTGCCATCCAGGTCATCAAGAAGAAGCTAGTGGGAT CTGTGAAGGCCCTGCAGAAACAGCATGTGTCTCTGGACACAGTGGTCACCAGTGAAGATGGAGATGCTAA CACCATGTGCAGTGCCCTGGAGGCCGTGTTCATCCATGGCTTGCATGCCAAGTACATCCGTGCTGAGGCT GGCGGCAAAAGGAAGAAGCACACTCATCAGAAGCCTCTGCCTCAGCCTGTCTTCTGGCCCCTTCTGAAAG CTGTCACCCACAAACACATTATCTCAGACTTGGAGCACCTGGTCTTCATCAATACAGACGTAGGCCGCTG CCGGGCCTGGCTGCGGTTGGCCCTCAATGATGGGCTGATGGAGTGCTACCTGAAGTTGCTCCTGCAGGAA CCTGCACGGTTGTGCGAGTACTATCAGCCCACGGCCCTGCTTCGAGATGCTGAGGAAGCCGAGTTCCTCC TGAGCTTCCTCCAAGGACTAACATCCTTGTCTTTTGAACTCTCCTACAAGTCAGCCATCTTAAATGAATG GACACTCACCCCACTGTCTCTCTCTGGGCTTTGCCCCCTCTCTGAACTGGACCCTCTCACTGTCTCTGGT GCAGAACTGCAACGGAAGGAGTCTCTGGATTCCATTTCCCATTCCTCAGGCTCTGAGGACATCGAAGTCC AACACTCAGGCCATAAGATCCGCAGGGACAGGAAGCTCACCGCCTCTTCCCTCAGCCTGGACACAGCCAG CTCATCCCAGCTATCCTGCAGCCTAAATTCTGACAGCTGCCTGCTCCAAGAGAATGGTCCCAAGAGTCCA GACCACTCTGAGGAACCCATGTCCTACGACTCAGATCTGGGCACAGCAAATGCTGATGACTCTGACAGGT CTCTGCAAGAGGTGTTGTCGGAATTCAGCAAAGCCCAGGTAAATTCTGCACCAAGCAGCGGACCAAGCCA AGAGTCAGACACCCCCATGTTCCAGACCCCCCTCTCCCTCCACAGCCTAGCTAACAGCACACACCTGCTC TTTGAAGGATCTGAAGAGCCCTTTCCTGCCCATACATCCTCTGGGACTTCAAGTGGCCACAAGCATCAGC CCCAGGAGAGTCCAGACATGCAGCCGCTAGGCACTGCCCAAGCTGGCCCTGCTGGGAGCACTTCAGACCA GCAGCCATCTAGTCCCGTGGCCGGAGCAGCTGACCAAGGAAGTGAGCCTTGGAAGGCCCTGGAATATGGC AGAGTTGGACCGAAGCTTGTGGTTTCCTCTCCTACGAGTCCGAAAGGCAAGAGCTGGATTTCTGAAGATG ACTTCTGCCGACCTCCCAAGGAGCATGCTCTGAAGAACACTTCAGATCTTTGCATATCTCCTCTGCAAGG GACTCCAGAGTTGAGGACTGCTCTCCACGGGCCGTTTTCTCAAGGACCAAGAAAGAGCTGCTCCCTGGGG GCCTTAGACAAAGCGTGTGTGTCCTCGCTGGACTACAGAAATGCCCAGACAGCTCCATCTCCAGCGGTGA CACAAGACCATAAAAACTTCTGTGTGGTGCATCGAAGGCAGATGGGGCTCTCTAACCCATTCCGGGGCCT CATGAAGCTGGGCACAGTAGCCCGGCGAGGAGCCATGGGCATCTGGAAGGAATTCTTTTGTGAGCTGTCC CCACTGGAGCTCCGCCTCTACCTGAGTGACGAGGAGCGCACCTGTGTGGAGAGCTGCTCCCTGCTACGGT GTGAGGCCGTGGGACCAGCACACAGCGATGGACGTTTTGAGTTGGTCTTCTCCGGCAAGAAGCTGGCTCT CCGTGCCTCCTCCCAGGACGAGGCTGAGGACTGGCTTGACCGTGTGCGGGAGGCTCTGCAGAAGGTGCGC CCTCAGCAGGAGGAGGAGTGGGTGAATATCCAGTACCCAGACCAGGCTGAGGATTCCCCAGAGGCGCCAC CAGACAACCTTCCCCCCTATTCAGCCCTGCTCCCAGAACATGCAGGTGCACAGGGAATACAGCCAAACTG GACATCTGCCCAGGTTCCAGAGCCAGATGCTATCAAAGAGTCTCTTTTGTACCTGTATGCAGACCGGACC TGGATTCCCTATATCTTTTCCCTGTCCTTGGAGTCTCTGAAATGTTTCCGTGTGAGAAACAATGAGAAGA TGCTAAGTGATAGCCATGGAGTGGAGACCATCCGGGACATCCTGCCAGACACCAGCCTTGGGGGCCCAGC CTTCTTCAAAATCATCACGGCCAAGGCTGTCCTCAAACTGCAGGCCAAGAACACTGAGGAGGCCGCCCAC TGGAGAGACCTAGTCAGGAAAGTGCTGGCGTCTTACTTAGAGTCAGTTCAGGAGGCTGTGACACTTGCTG GGAGTCTGGATGAAAACTGTCAGGAGGTCCTGAAGTTTGCCACCAGGGAGAATGGCTTCCTGCTACAGTA CCTTGTGGCCATCCCGACCGAGAAGGGCCTCGACTCCCAGGGCTGCTTCTGTGCAGGTTGCTCCCGGCAG ATCGGCTTCTCCTTTGTACGACCTAAGCTCTGTGCCTTCTCTGGCCTATATTACTGTGACTTCTGCCACC AAGACGATGCCTCGGTGATTCCAGCCAGAATCATTCATAACTGGGACCTCACAAAGCGCCCGGTCTGCCG GCAGGCCCTGAAGTTCCTGGCCCAGATCCGGGCCCAGCCCCTCATCAACCTGCAGCTGGTGAACGCCTCT CTGTATGAGCATGTAGAGCGCATGCACCTCATTGGCAGGAGCCGGGAGCAGCTGAAACTTCTGGGGGACT ACCTGGGCCTGTGCCGAAGTGGTGCTCTGAAGGAGTTGAGCAAAAGGCTAAGCCACAGGAATTACCTCTT GGAGTCTCCTCACAAATTCAGTGTTGCTGACCTCCAGCAGATTGCAGAGGGGGTGTATGAAGGATTCCTC AAAGCCCTGATTGAATTCGCCTCCCAGCATGTCTACCACTGTGACCTGTGCACCCAGCGAGGCTTCATCT GCCAGATCTGCCACCACCAGGACATCATCTTTCCCTTTGAGTTTGACACCACAGTCAGGTGTGCTGAATG CAGAACTGTCTTCCATCAGAGCTGCCAGGCTGTGGTGAGGAAGGGCTGTCCCCGCTGTGCCCGCCGGCGC AAGTACCAGGAACAGAACACTGTCAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001009677 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001009677.1, NP_001009677.1 |
| RefSeq Size | 5050 bp |
| RefSeq ORF | 3180 bp |
| Locus ID | 303584 |
| UniProt ID | Q5PQS0 |
| Gene Summary | Proposed to act as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Seems to be involved in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (By similarity). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:17404618). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7. May have a role in sialyl-lex-mediated transduction of apoptotic signals (By similarity). Involved in bone resorption (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR212281 | Plekhm1 (Myc-DDK-tagged ORF) - Rat pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), (10 ug) |
CNY 17,010.00 |
|
| RR212281L3 | Lenti ORF clone of Plekhm1 (Myc-DDK-tagged ORF) - Rat pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), (10 ug) |
CNY 18,620.00 |
|
| RR212281L4 | Lenti ORF clone of Plekhm1 (mGFP-tagged ORF) - Rat pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), (10 ug) |
CNY 20,430.00 |
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