Rufy3 (NM_001025127) Rat Untagged Clone

CAT#: RN212245

Rufy3 (untagged ORF) - Rat RUN and FYVE domain containing 3 (Rufy3), (10 ug)



  "NM_001025127" in other vectors (3)

CNY 6,080.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Ripx; Singar1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN212245 representing NM_001025127
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCCCTGACGCCTCCGACTGATATGCCAACCCCCACCACTGACAAGATAACCCAGGCTGCCATGG
AGACCATCTACCTCTGCAAATTCCGGGTGTCTATGGATGGAGAATGGCTCTGCCTTCGAGAGCTGGATGA
CATCTCCCTTACACCTGACCCAGAGCCTACCCATGAAGATCCTAATTATCTCATGGCTAACGAGCGCATG
AACCTGATGAACATGGCGAAGCTGAGCATCAAGGGCTTGATTGAGTCGGCTCTGAACCTGGGGCGGACCC
TGGACTCTGACTACGCACCTCTCCAGCAGTTCTTCGTGGTGATGGAACACTGCCTGAAACATGGCTTGAA
AGCCAAGAAAACTTTTCTTGGACAAAATAAGTCCTTCTGGGGTCCTCTAGAGCTGGTAGAAAAGCTTGTT
CCAGAAGCTGCAGAGATAACAGCAAGTGTAAAAGATCTCCCAGGACTCAAGACACCAGTTGGCAGAGGAA
GAGCCTGGCTTCGGTTGGCATTAATGCAAAAGAAGCTTTCTGAGTACATGAAGGCCTTGATCAATAAGAA
GGAACTTCTCAGTGAGTTCTATGAAGCCAATGCTCTCATGATGGAAGAAGAAGGCGCAATTATTGCTGGC
CTCCTGGTCGGTCTGAATGTCATCGATGCCAATTTCTGCATGAAAGGAGAAGACCTGGACTCTCAGGTTG
GCGTTATAGACTTTTCAATGTATCTCAAAGATGGGAACAGCAGTAAAGGCAGTGAAGGGGATGGACAGAT
TACTGCGATTCTGGACCAGAAAAACTATGTAGAAGAACTCAACAGACATCTGAATGCTACTGTAAACAAC
CTTCAGGCAAAAGTAGATGCGTTAGAAAAATCCAACACGAAATTGACAGAGGAACTTGCCGTCGCCAACA
ACAGAATTATTACCTTACAAGAAGAAATGGAACGGGTTAAAGAAGAAAGTTCCTATCTACTGGAATCCAA
TCGGAAGGGTCCTAAGCAAGACAGAACTGCAGAAGGGCAAGCGCTGAGCGAAGCCAGAAAGCATCTAAAG
GAGGAGACACAGTTACGATTGGACGTTGAAAAGGAGCTGGAGCTGCAGATCAGCATGAGGCAGGAGATGG
AACTGGCTATGAAGATGCTGGAGAAGGATGTCTGTGAGAAGCAGGATGCCCTGGTGTCCCTGCGGCAGCA
GCTGGATGATCTCCGAGCTCTTAAACACGAGCTTGCCTTTAAGCTGCAGAGTTCAGACCTAGGAGTGAAA
CAGAAAAGTGAATTAAACAGTCGCTTGGAAGAGAAGACCAATCAGATGGCTGCCACCATTAAACAGCTGG
AGCAAAGTGAAAAAGATTTGGTGAAACAGGCAAAGACCTTAAATAGTGCAGCAAATAAACTGATCCCAAA
ACATCATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001025127
Insert Size 1410 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001025127.1, NP_001020298.1
RefSeq Size 1903 bp
RefSeq ORF 1410 bp
Locus ID 360921
UniProt ID Q5FVJ0
Gene Summary Plays a role in the generation of neuronal polarity formation and axon growth (PubMed:17439943). Implicated in the formation of a single axon by developing neurons (PubMed:17439943). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (PubMed:17439943). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery (By similarity). Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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