Tnc (NM_053861) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212144 representing NM_053861 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCCGTGRCCTGGCTATTGCCAGGCATCTTCCTAGCTTTGTTTGCCCTCACTCCTGAAGGTGGGG TCCTCAAGAAAGTCATCAGGCACAAGCGAGAGAGTGGGCTGAACTTGACCTTGCCAGAGGAGAATCAGCC AGTGGTGTTCAACCATGTCTACAACATCAAGTTGCCCATGGGTTCTCAGTGCTCAGTGGATCTAGAGTCA ACGAGTGGAGAGAAAGACCTGAGCCCCACACCAGAGTCCAGTGGAAGCTTCCAGGAGCATACAGTGGATG GGGAAAATCAGATTGTGTTCACACACCGCATCAACATCCCTCGTCGGGCCTGTGGCTGTGCCGCAGCTCC AGATGTGAAGGAGCTCCTGAGCAGACTGGAGGAACTGGAGATGTTGGTATCTTCTCTAAGGGAGCAGTGC ACCATGGGTACAGGCTGTTGCCTCCAACCTGCAGAAGGCCGTCTGGACAGCAGGCCCTTCTGCAGCGGCA GGGGTAACTTCAGTGCTGAAGGTTGTGGCTGTGTCTGTGAACCAGGCTGGAAAGGTCCCAACTGCTCTGA GCCTGAATGCCCTGGAAACTGTAATCTCAGAGGCCAGTGCCTTGATGGACAGTGTATCTGTGACCAGGGT TTCACTGGGGAAGACTGCAGCCAGCTAGCCTGTCCCAATGACTGCAATGACCAGGGCAAGTGTGTGAATG GGGTCTGTGTGTGCTTCGAAGGCTATGCCGGCCTTGACTGTGGCCTGGAAGTCTGCCCAGTGCCGTGCAG CGAGGAACACGGGATGTGTGTGGATGGCAGGTGTGTGTGCAAAGATGGCTTTGCTGGTGAAGACTGCAAT GAGCCCCTTTGCTTCAACAACTGCAACAACCGTGGGCGGTGTGTGGAGAACGAATGCGTCTGTGATGAGG GCTTCACGGGCGAAGACTGCAGCGAGCTCATTTGCCCCAATGACTGCTTCGACCGAGGTCGCTGCATCAA CGGCACCTGCTACTGTGAAGAAGGTTTCACAGGTGAAGACTGTGGTGAGCTCACCTGCCCCAACAACTGT CAGGGCCATGGCCAGTGTGAAGAGGGACAGTGTGTTTGCAACGAGGGCTTTGCAGGGGCAGACTGCAGTG AAAAGCGGTGTCCCGCAGACTGTCACCACCATGGCCGCTGTCTCAACGGACAGTGTGAGTGTGACGATGG GTTCACAGGGGCTGACTGTGGGGAACTCCAGTGCCCCAATGGCTGCAGTGGGCATGGCCGCTGTGTCAAT GGGCAGTGTGTGTGTGACGAGGGCTACACCGGAGAAGACTGTAGCCAACGGCGATGCCCCAATGACTGCC ACAACCGGGGTCACTGTGTACAGGGCAAATGCATATGTGAGCAAGGCTTCAAAGGCTTTGACTGTAGTGA GATGAGCTGTCCCAATGACTGCCACCAGCATGGCCGCTGTGTGAATGGCATGTGCATCTGTGATGATGAC TACACTGGGGAAGACTGCAGAGACCGCCGCTGTCCCCGGGACTGTAGCCAGCGGGGGCGCTGTGTGGACG GACAGTGCATTTGTGAGGATGGTTTCACTGGTTCTGACTGTGCTGAGCTCTCCTGCCCTGGTGACTGCCA CGGCCATGGCCGCTGTGTGAATGGCCAATGCATCTGCCACGAGGGCTTCACCGGCRAAGACTGCAAAGAG CAAAGGTGCCCCAGTGACTGCCATGGCCAAGGCCGCTGTGAGGACGGCCAATGTATCTGCCATGAGGGCT TCACAGGCCTGGACTGTGGGCAGCGCTCCTGTCCCAATGACTGCAGCAACCAGGGACAATGTGTGGCAGG CCGCTGCATCTGCAATGAAGGCTACACAGGGGTAGACTGCTCTGAGGTGTCCCCTCCCAAAGACCTTATT GTGACAGAAGTAACAGAGGAGACTGTARATCTGGCATGGGACAATGAGATGCGGGTCACTGAGTACCTCA TTATGTACACACCCACCCATGCTGATGGCCTGGAGATGCAGTTCCGTGTGCCTGGGGACCAGACATCTAC CACCATCCGGGAGCTGGAGCCAGGAGTGGAGTACTTCATTCGTGTGTTCGCCATCTTGGAGAACAAGAGG AGCATCCCTGTCAGTGCCAGAGTTGCCACCTACTTGCCTGCACCTGAAGGCCTAAAATTCAAGTCTATCA AGGAGACATCTGTGGAAGTAGAGTGGGATCCTCTGGACATCGCTTTTGAAACGTGGGAGATCATTTTCAG AAATATGAACAAAGAAGATGAGGGAGAGATCACAAAAAGCTTGAGGAGGCCAGAGACCTCCTACCGCCAA ACTGGCCTGGCTCCTGGCCAAGAATATGAAATATCTCTTCACATTGTGAAAAACAACACCCGAGGCCCTG GCTTGAAGAAAGTGACCACAACTCGCCTGGATGCCCCCAGCCAAATTGAGGTGAGAGACATCACAGACAC TACAGCACTGATCACCTGGTCCAAGCCCTTGGCTGAAATTGATAGCATTGAGCTCTCCTATGGCATCAAG GATGTGCCTGGAGACCGGACCACCATCGACCTCACACATGAAGACAACCAGTACTCCATCGGGAACCTGA AACCTGACACGGAGTATGAGGTGTCCCTCATCTCCCGCAGAGTGGACATGGCAAGCAACCCTGCCAAGGA GACCTTCATCACAGGCCTGGATGCTCCCAGGAATCTCCGTCGCGTCTCACAGACAGACAACAGCATCACC TTGGAGTGGAGGAATGTCAAGGCAGACATTGATAGTTATAGAATTAAGTATGCACCTATCTCTGGAGGTG ACCATGCTGAGATAGATGTTCCAAAGAGCCAGCAAGCCACAACCAAAACCACACTCACAGGTCTAAGGCC AGGAACTGAATATGGAGTGGGCGTCTCTGCTGTTAAGGGAGACAAGGAGGGCGATCCAGCAACCATCAAT GCAGCCACAGAAATTGATGCACCCAGGGACTTACAGGTGTCTGAGACCACACAAGACAGTCTGACTTTAT TCTGGAAGACACCTCTGGCCAAGTTTGATCGTTACCGCCTCAACTACAGCCTCCCCACAGGCCAGTCAAT AGAGATTCAGCTGCCAAAGGATGCCACCTCCCATGTCCTGACAGACCTGGAGCCAGGGCAAGAATACACT GTTCTCCTCACCGCTGAGAAGGGCAGACATAAGAGCAAGCCTGCGCGTGTGAAGGCATCCACAGAAGAAG TACCTTCTCTGGAAAATCTCACCGTGACTGAGGCCGGCTGGGATGGCCTCAGACTCAACTGGACTGCAGA TGACCTGGCCTATGAGTACTTTGTTATTCAGGTACAGGAAGCCAACAAGGTGGAGACTGCTCACAACTTC ACAGTACTTGGTAACCTCCGGACTGCAGACATCCCGGGCCTCAAGGCTGCCACTCCTTATAGAGTCTCCA TTTATGGGGTAGCTCGGGGCTATAAAACACCCGTGCTCTCTGCTGAGACCTTCACAGGGAAAACTCCCAG TTTGGGAGAGGTGACTGTGGCTGAGGTGGGCTGGGATGCCCTCAAGCTCAACTGGACTGCTCCAGAAGGA GCCTATACGAACTTTTTCATTCAGGTGCTAGAGGCTGACATGACCCAGACTGTCCAGAACCTCACAGTCC CAGGGGGACTGAGGTCAGTGGACCTACCTGGGCTCAAAGCAGCCACCCGCTATTACATCACCGTTCGAGG GGTCACCCAGGACTTCAGCACAGCCCCTCTCTCTGTTGAGGTCTTGACAGAGGAGATCCCTCAACTGGGA GGCTTGTCCGTGACTGAGGTCAGCTGGGACAGCCTTACACTCAACTGGACCACAGATGATCTGGCCTATA AGCACTTTATCATTCAGGTGCAGGAGGCCAACAATGTAGAGGCTGCTCAGAACCTCACAGTATCTGGTAG CCTCAGAGTTGTGGACATCCCAGGCCTCAAGGCTGATACCCCTTATAGAGTCTCCATCTATGGGGTGATC CAGGGCTATAGAACACCAATGCTCTCTGCTGATGTCTCCACAGCCAAAGAACCTGAAATTGGAAACTTAA ATATTTCTGATGTAACTCCTGAGAGCTTCAATCTCTCCTGGACAGCTACCGACGGGATCTTCGACATGTT TACTATCGAAATTATTGATTCTAATAGGTTGCTGCAGACAGCAGAACATAATATATCTGGTGCTGAACGA ACTGCCCACATCTCGGGCCTTCCACCTAGTACTGATTTCATTGTCTACCTCTCTGGAATTGCTCCCAGCA TCCGTACCAAAACCATCAGTACCACAGCCACCACAGAAGCTGAACCGGAAGTTGACAACCTTCTAGTTTC AGATGCTACTCCAGACGGTTTCTGTCTGTCCTGGACTGCTGATGAAGGGATATTCGACAGTTTTGTTATC AGGATCAGAGATACCAAAAAGCAATCTGAACCACAAGAAATAACCCTCCCTTCCCCCGACCGTACCAGGG ACATAACAGGTCTCAGAGAGGCCACTGAGTACGAAATTGAACTCTATGGAATAAGCCGTGGAAGGCGATC CCAGCCAGTCAGTGCCATAGCAACAACAGCCATGGGCTCTCCGAAGGAAATCATGTTCTCAGACATCACT GAAAATGCAGCCACAGTCAGCTGGAGGGCACCGACTGCTCAGGTGGAGAGTTTCCGGATTACTTATGTGC CTGTAACAGGAGGTCCCCCATCCATGGTGACCGTGGATGGGACGGATACTGAGACCCGACTGGTGAGGCT CACCCCTGGAGTGGAGTACCACGTCAGTGTGATTGCCATGAAGGGATTTGAAGAAAGTGATCCGGTCTCG GGGTCTCTAATCACAGCTCTCGATGGTCCATCTGGCCTTCTGACAGCCAACATCACAGACTCAGAAGCCT TGGCCATGTGGCAGCCGGCCATTGCCACTGTGGACAGTTATGTCATCTCCTACACAGGGGAGAGAGTGCC AGAAATTACCCGCACAGTGTCTGGGAATACAGTGGAGTTTGAACTACATGACCTGGAGCCTGCCACAGAG TACACACTGAGTGTCTTTGCAGAGAAAGGTCACCAGAAGAGTTCTACCATCGCTACGAAATTTACCACAG ACCTGGATTCCCCAAGAGAATTGACTGCTACTGAGGTTCAGTCAGAAACTGCCTTCCTCACCTGGAGACC TCCCCGGGCATCGGTCACTGGATACCTCCTGGTCTATGAGTCTGTGGATGGTACAGTCAAGGAAGTCATT GTGGGGCCTGACACCACCTCCTACAGCCTGGCAGACCTGAGCCCATCCACCCACTACACAGCAAGAATCC AGGCATTGAGTGGGTCCCTGAGGAGCAAACTGATCCAAACCATCTTCACCACAATTGGACTCCTATATCC ATTCCCTAGGGATTGCTCTCAAGCAATGTTGAATGGCGACACCACCTCTGGCCTCTATACCATCTATATA AATGGTGACAAGACTCAAGCACTGGAAGTCTACTGTGATATGACCTCTGATGGAGGTGGATGGATCGTTT TCCTGAGACGCAAAAATGGACGTGAGGACTTCTATCGAAACTGGAAGGCCTATGCCACTGGGTTTGGAGA CCGCAGAGAAGAATTCTGGCTTGGACTGGATAACCTGAGCAAAATCACAGCCCAAGGGCAATATGAGCTC CGGGTGGACCTACAAGACCATGGGGAGTCAGCCTATGCTGTGTATGACAGGTTCAGTGTTGGAGATGCCA AGAGTCGCTACAAGCTGAAGGTAGAAGGATACAGTGGAACGGCAGGTGACTCCATGAACTATCACAATGG TAGATCCTTCTCTACCTATGACAAGGACACAGACTCAGCCATCACCAACTGTGCCCTGTCCTACAAAGGA GCTTTCTGGTATAAGAACTGTCATCGTGTCAACCTGATGGGCAGATATGGGGACAATAATCACAGTCAGG GTGTTAACTGGTTCCACTGGAAGGGCCATGAGTACTCAATCCAGTTTGCGGAGATGAAACTAAGACCCAG CAACTTCCGAAATCTGGAAGGCAGGCGTAAGCGGGCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053861
Insert Size	6060 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053861.1, NP_446313.1
RefSeq Size	6155 bp
RefSeq ORF	6060 bp
Locus ID	116640
Gene Summary	extracellular matrix glycoprotein; inhibits the attachment of fibroblasts to adhesive proteins [RGD, Feb 2006]

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