Usp47 (NM_001107542) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212065 representing NM_001107542 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAGGAGATAGAAAATGCTGCTGAAGAACCTAGAGTCT TATGTATAATACAGGACACTACTAACTCAAAGACAGTGAATGAACGGATCACAGTGAATTTACCAGCATC TACCCCAGTCAGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACATAAACGGAACCTTTGATTTG ACATGGGGAAATGGAATCAATACTGCTGATTTGGCACCACTGGATCACACCAGTGACAAGTCTCTTCTTG ATGCTAATTTTGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGAGAACAGCCTCAGAT GCTATTGGATGACTCCAGTCACGTGGACGACAGCGTGCATGACAGGTTTATAGGTCCACTTCCAAGAGAA GGGTCTGTGGCTTCTACCAGTGATTATGTCAGCCAGAGCTACTCCTATTCATCAATTTTGAACAAGTCAG AAACTGGTTATGTGGGATTAGTAAACCAGGCGATGACGTGCTATTTGAATAGCCTTTTGCAAACACTTTT TATGACTCCTGAATTTAGGAATGCCTTATATAAGTGGGAGTTTGAAGAGTCTGAAGAAGATCCTGTAACG AGCATCCCATACCAGCTTCAGAGGCTTTTTGTTCTGTTACAAACCAGCAAAAAGAGAGCAATTGAGACCA CAGATGTCACAAGGAGCTTTGGATGGGATAGCAGTGAGGCTTGGCAACAGCATGATGTACAAGAACTGTG CCGTGTTATGTTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTG TACCAAGGCAAACTGAAAGACTATGTGAGGTGTCTGGAATGCGGTTATGAAGGCTGGAGAATTGACACGT ACCTTGATATTCCACTGGTCATCCGACCTTATGGGTCTAGCCAAGCATTTGCCAGTGTGGAAGAAGCATT GCATGCATTTATTCAACCAGAGATCTTGGATGGCCCAAATCAATACTTCTGTGAACGTTGTAAGAAGAAG TGCGATGCTCGTAAGGGCCTTCGGTTTTTGCACTTTCCCTATCTGCTGACTTTACAGTTGAAAAGGTTTG ATTTTGATTATACAACCATGCATAGAATTAAACTGAATGATCGAATGTCATTTCCAGAGGAGCTGGATAT GAGCACATTTATTGATATTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGATAGTGGAGCGGAA AATGAAGGCAGTTGCCACAGCGACCAGATGAGCAATGATTTCTCCACGGATGATGCTGTTGATGAAGGGA TATGCCTGGAGAGCAGTACTGGGAGTGAGAAGATTTCAAAACCTGGCCTGGAAAAGAACTCCCTGATGTA TGAGCTCTTCTCGGTGATGGTACATTCAGGGAGTGCTGCTGGGGGACACTATTATGCTTGCATAAAGTCT TTCAGCGACGATCAGTGGTACAGTTTCAATGACCAACATGTGAGCAGGATAACACAAGAAGACATTAAGA AGACACACGGTGGGTCTTCAGGAAGCAGAGGCTACTACTCCAGTGCTTTTGCAAGCTCAACAAATGCATA TATGCTGATATACAGACTGAAGGATCCAACAAGAAATGCAAAATTTCTGGAAGTGGATGAGTACCCAGAA CACATTAAGAACCTGGTGCAGAAGGAGAGAGAATTGGAAGAACAGGAAAAGAGGCAACGAGAAATTGAAC GAAACACATGCAAGATAAAATTATTCTGTTTACATCCTGTGAAACAAGTAATGATGGAAAACAAGTTGGA GGTTCATAAGGACAAGACATTAAAGGAAGCAGTAGAAATGGCTTATAAGATGATGGATTTAGAAGACGTG ATTCCCTTGGATTGCTGTCGCCTGGTAAAATATGATGAGTTTCATGATTACCTGGAACGATCCTATGAGG GAGAAGAAGATACGCCAATGGGGCTGCTTCTAGGAGGAGTCAAGTCAACGTACATGTTTGATCTGCTGTT GGAGACAAGAAAGCCTGATCAAATTTTTCAGTCTTATAAACCTGGAGAAGTGATGGTGAAAGTTCATGTT GTCGATCTGAAGGCAGAGACTGTCGCTGCTCCTGTAACAGTGCGTGCTTATTTAAATCAGACAGTTTTGG AGTTCAAACAGCTGATCTCAAAGGCCACACACTTACCTGCAGAACCCATGAGGATCGTGCTGGAGCGCTG CTACAACGACCTGCGCCTGCTCAGCATGCCCAGCAAGACCCTGAAGGCTGAGGGCTTCTTTAGAAGTAAC AAGGTGTTTGTTGAAAGCTCTGAGACAGTAGATCACCAGGCAGCCTTCACAGACTCCCACTTGTGGAAAC TCCTGGATCGACATGCAAATACAATCCGGTTATTTGTTTTGCTGCCTGAACAATCTCCAGGGTCTTATTC GAAAAGGACAGCATACCAGAAAGCTGGAGGGGATTCTGGTAATGTGGACGATGACTGTGAAAGAGCCAAG GGACCTGCAGGAAGTTTAAAGTCTGTGGATGCCATTCTAGAAGAGAGCACTGAAAAACTCAAAAGCCTGT CACTGCAGCAGCAGCAGGATGGGGACAACGGAGACAGCAGCAAAAGCACTGAGACAAGTGACTTTGAGAA TATTGAATCCCCTCTCAATGAGAGGGGCTCTTCCACCTCTGTGGATAATCGAGAACTTGAACAACACATT CAGACTTCTGACCCAGAAAACTTTCAGTCTGAAGAACGCTCAGACTCAGATGTGAATAATGACCGGAGTA CAAGTTCGGTAGACAGCGATATTCTTAGCTCTAGTCACAGCAGCGACACTTTGTGCAATGCAGACAGCGC TCAGATTCCTTTGGCCAATGGACTCGATTCTCACAGTATCACAAGTAGTAGAAGAACTAAGGCAAACGAA GGGAAAAAGGAGACATGGGACACAGCTGAAGAAGACTCTGGGACTGACAGTGAATATGATGAGAGTGGCA AGAGCAGGGGGGAGATGCAGTACATGTACTTCAAAGCTGACCCCTACACTGCGGACGAAGGCTCTGGGGA AGGACACAAATGGTTGATGGTGCATGTTGATAAGAGAATCACCCTGGCAGCTTTCAAACAGCATTTAGAG CCTTTTGTTGGAGTCCTCTCCTCTCACTTCAAGGTTTTTCGAGTGTACACCAGCAATCAAGAGTTTGAGA CCGTCCGGTTGAATGAGACCCTTTCATCATTTTCAGATGACAATAAGATCACAATCCGACTGGGGAGAGC ACTGAAGAAGGGAGAGTACAGAGTTAAAGTGTGCCAGCTCTTAGTCAATGAGCAAGAGCCATGCAAGTTT CTGCTAGACGCCGTATTTGCTAAAGGCATGACTGTGCGGCAATCGAAAGAGGAGCTGATTCCTCAACTCA GGGAGCAGTGTGGCTTAGACCTCAGCATCGACAGGTTTCGTCTAAGGAAGAAAACATGGAAGAATCCTGG CACTGTTTTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCTAGCAACTGGGAAGTTTTCCTG GAAGTTCTTGATGGTGTGGAGAAGATGAAGTCCATGTCACAGCTTGCCATTTTAACCAGACGGTGGAGGC CTTCGGAGATGAAGTTGGATCCCTTCCAGGAGCTTGTGTTGGAGAGCAATAGTGTTGATGAGCTTCGAGA GAAGCTGAGTGAAATCAGTGGGATTCCTTTGGAAGACATTGAATTTGCCAAGGGTAGGGGAACCTTCCCC TGTGATATTTCTGTCCTTGATATTCATCAAGATTTGGACTGGAACCCTAAAGTCTCTACCTTGAATGTGT GGCCTCTTTATATCTGTGATGATGGTGCCGTCATATTTTATAGGGATAGAACAGAAGAAGTAATGGAATT GACAGATGAGCAAAGGAATGAACTGATGAAAAAGGAAAGCAGCCGACTGCAGAAGACGGGACATCGTGTG ACATACTCCCCTCGCAAAGAGAAAGCACTCAAGATCTACCTGGATGGGGCGCCAAATAAAGACGTAGCTC AAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107542
Insert Size	4068 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107542.2, NP_001101012.2
RefSeq Size	5691 bp
RefSeq ORF	4068 bp
Locus ID	308896

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