Pole (NM_001107152) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	POLE1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212034 representing NM_001107152 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGAGGAACAGTGGACGGAGGCATCCCGAGCCGGGCGCGGACAGCGAAGGCAGCCGGGATGATG GTCCCTCCTCTTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACGGATAAGATGGACTTACG GTTTGGTTTTGAAAGGTTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTTATCAACATGCACCCTACAGAG ATTTTAGATGAAGACAAACGCTTAGTCAGTGCAGTGGATTACTACTTCATTCAAGATGATGGAAGCAGAT TTAAGGTGGCCTTGCCCTACAAGCCATATTTCTACATTGCAGCTAGAAAGGGTTGTGATCGAGAAGTTTC ATCTTTTCTATCCAAGAAGTTTCAGGGTAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGACCTGGAC TTGCCAAACCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGATCTTG TCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGACGAGTA CACAACAATGCTCTCCAGTATTCTGCAAGGAGGCAGTCTAATTACTGATGAGGAGGAAACCTCTAAGAAG ATAGCTGACCAATTGGACAACATAGTGGACATGCGGGAGTATGATGTTCCCTACCACATTCGCCTCTCCA TTGACCTCAGAATCCATGTGGCCCACTGGTACAATGTTAGATTTCGAGGAAATGCTTTTCCTGTGGAAAT TGCCCGACGAGATGATCTCGTCGAACGACCTGATCCTGTGGTTTTGGCATTTGACATTGAGACAACCAAA CTACCTCTCAAATTCCCTGATGCTGAGACAGATCAGATTATGATGATCTCCTATATGATTGATGGCCAGG GCTACCTCATCACTAACAGGGAGATTGTTTCAGAAGACATTGAAGATTTTGAGTTCACCCCCAAGCCAGA ATATGAAGGGCCTTTCTGTGTCTTCAATGAACCTGATGAGGTCCATCTGATCCAGAGATGGTTTGAGCAT ATCCAGGAGACCAAACCTACCATTATGGTCACCTACAATGGGGATTTTTTTGACTGGCCGTTTGTGGAGG CCAGGGCAGCAATTCATGGCCTAAGCATGTACCAGGAGATAGGCTTCCAGAAGGATAGCCAGGGGGAATA TAAGGCACCACAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGTGGGC AGTCATAATCTCAAGGCAGCTGCCAAAGCCAAACTTGGCTATGACCCTGTGGAGCTGGACCCTGAGGACA TGTGCCGTATGGCCACTGAACAGCCCCAGACTCTGGCCACTTACTCAGTGTCAGATGCTGTTGCTACTTA TTACCTGTACATGAAATATGTGCACCCCTTCATATTCGCCCTCTGCACCATTATTCCCATGGAACCTGAT GAGGTGCTGCGGAAGGGCTCTGGAACATTGTGCGAAGCCTTGCTGATGGTGCAAGCCTTCCATGCCAACA TTATCTTTCCCAATAAGCAAGAGCAGGAGTTCAACAAGCTGACAGATGATGGCCACGTGCTAGATGCTGA GACCTACGTTGGGGGCCATGTGGAGGCCCTAGAGTCTGGTGTCTTCCGAAGTGATATCCCCTGCCGGTTT AGGATGAATCCTGCAGCCTTTGATTTCCTGCTGCAGCGAGTTGAGAAGACTATGCGCCATGCCATTGAAG AAGAAGAGAAGGTGCCTGTGGAACAAGCCACCAACTTCCAAGAGGTGTGTGAGCAGATTAAGACCAAACT CACCTCCCTAAAAGATGTTCCTAACAGAATTGAATGTCCTCTAATCTACCATCTAGATGTGGGGGCCATG TATCCTAACATAATCCTCACCAACCGCCTGCAGCCTTCTGCCATAGTGGATGAGGCCACCTGTGCTGCCT GTGACTTCAATAAGCCTGGAGCAACTTGTCAGAGGAAAATGGCCTGGCAATGGAGGGGAGAATTCATGCC AGCCAGTCGTAGTGAGTACCATCGAATCCAGCATCAGCTAGAGTCGGAGAAGTTCCCCCCTTTGTTCCCA GAGGGGCCAGCAAGGGCCTTTCATGAGCTGTCCCGTGAAGAACAGGCTAAATACGAGAAACGGAGGCTGG CAGATTATTGCCGGAAAGCCTATAAGAAGATCCATATGACCAAGGTAGAAGAGCGCCTGACTACCATCTG CCAGCGGGAAAACTCATTTTATGTGGACACAGTGCGGGCCTTCAGAGACAGGCGCTATGAATTCAAAGGA CTGCACAAGGTGTGGAAGAAGAAGCTCTCGGCGGCTGTAGAGGTGGGCGATGCATCAGAGGTGAAGCGCT GCAAGAACATGGAGATCCTTTATGATTCGCTGCAGCTGGCTCACAAATGCATCCTGAACTCATTCTACGG CTATGTTATGCGCAAAGGAGCTCGCTGGTACTCCATGGAGATGGCTGGTATCGTCTGCTTTACAGGAGCC AACATCATCACCCAGGCAAGAGAGCTGATTGAGCAGATCGGGAGGCCTTTAGAACTGGACACGGACGGAA TATGGTGCGTTCTACCCAATAGCTTTCCTGAAAATTTTGTCATCAAGACAACCAATGTGAAAAAACCCAA ACTGACCATCTCCTATCCTGGTGCCATGTTGAACATCATGGTCAAGGAAGGCTTTACCAACCACCAGTAC CAGGAACTAACAGAGCCTTCGTCTCTCACCTATGTCACCCGCTCTGAGAACAGCATCTTTTTTGAAGTTG ATGGACCATACCTTGCTATGATCCTTCCAGCCTCCAAGGAAGAAGGCAAGAAGCTGAAGAAAAGATATGC TGTATTCAATGAAGATGGCTCCTTGGCTGAACTGAAAGGTTTTGAGGTGAAACGCCGAGGGGAGTTGCAG TTGATTAAAATCTTCCAGTCCTCAGTGTTTGAGGCCTTCCTCAAGGGCAGCACACTAGAGGAGGTGTATG CCTCTGTGGCCAAAGTGGCTGACTACTGGCTAGATGTGCTCTATAGCAAGGCTGCTAATATGCCCGATTC TGAATTATTCGAACTGATTTCTGAGAACCGCTCCATGTCACGGAAGCTAGAAGATTATGGGGAGCAGAAG TCTACGTCCATCAGCACAGCAAAGCGCCTGGCTGAGTTCCTGGGAGACCAGATGGTCAAAGATGCTGGAC TGAGCTGCCGCTACATTATCTCCCGAAAGCCAGAGGGATCTCCTGTCACTGAGAGGGCCATTCCACTTGC CATTTTCCAAGCAGAGCCTACAGTGAGGAAACATTTTCTCCGGAAATGGTTAAAGAGTTCATCACTTCAA GACTTTGATATTCGGACAATTCTGGACTGGGACTACTACATAGAGCGGCTGGGGAGTGCCATCCAGAAAA TCATCACCATCCCTGCAGCTCTACAGCAGGTGAAGAACCCAGTTCCACGTGTCAAACATCCAGACTGGCT GCACAAAAAGCTACTAGAGAAGAATGATATCTACAAACAGAAGAAGATCAGTGAGCTCTTTGTGCTTGAA GGAAAGAGACAGATTGTGATGGCCCAGGCTTCAGAAAACAGTCTGAGTCTCTGCACTCCAGACATGGAGG ACTTTGGACTCACAAAGCCATATCACTCTACAGTTCCAGTTGCTACTAAGAGGAAGCGAGTCTGGGAGAG CCAAAAGGAGTCTCAGGATATTACACTAACTGTGCCCTGGCAAGAGGTCTTAGGGCAGCCTCCCTCCCTT GGAACCACACAGGAAGAGTGGTTGGTCTGGCTCCAGTTCCACAAGAAAAAGTGGCAGCTGCAGGCTCAAC AGCGCCTAGCCCGCAGGAAGAAACAGCGCTTAGAGTCAGCAGAAGATATGCCAAGGCTTGGGGCTATCCG AGAGGGGCCTGCCACAGGACTGGGGAGCTTTCTGCGACGGACCGCCCGCAGCATCATGGACCTCCCCTGG CAGATCATACAGATCAGTGAGACCAGACAGGCTGGTCTGTTCCGGCTGTGGGCTATCATTGGCAGCGACT TGCACTGCATCAAGCTGAGTATCCCTCGAGTATTCTATGTAAACCAGCGGGTAGCCAAAGCAGAGGATGG GCCTTCGTATCGAAAGGTGAATCGGGCTCTTCCTCGTTCCAACATCGTCTACAATCTCTATGAGTACTCA GTACCAGAGGACATGTACCAAGAACACATCAACGAGATCAACACTGAGTTGTCAGCACCAGACATTGAGG GCGTGTATGAGACTCAGGTCCCATTGTTATTCCGGGCCCTGGTGCAGCTGGGCTGTGTGTGTGTGGTCAA CAAACAGCTGGCAAGGCAACTTTCAGGCTGGGAAGCTGAAACCTTTGCCCTCGAGCACCTTGAAATGCGT TCTCTGGCCCAGTTCAGCTACTTGGAACCAGGGAGTATCCGCCACATCTACCTGTACCATCACACACAGG GCCACAAGGCACTCTTTGGGGTCTTTATCCCCTCACAGCGAAGAGCATCTGTGTTTGTGTTGGATACTGT GCGCAGCAACCAAATGCCAGGGCTCAGTGCCCTATACTCAGCAGAACACAGCCTGCTGCTGGACAAGGTG GGCCCCAACCTCCTGCCTCCCCCAAAACACACCTTTGAAGTTCGTGCTGAAACCAACCTGAAGACCATCT GCAGAGCCATCCAACGCTTCCTGCTTGCCTACAAGGAAGAGCGCCGGGGGCCCACGCTCATCGCTATCCA GTCTAGCTGGGAACTGTGTAGGCTGACCAGTGAGATTCCAGTCTTAGAGGAGTTCCCACTAGTGCCTGTC CGAGTGGCTGACAAGATCAGCTATGCAGTCCTGGACTGGCAGCGCCACGGAGCTCGCCGAATGATCCGGC ACTACCTCAATTTAGACATTTGCCTTTCACAGGCCTTTGAGATGAGCAGGTACTTCCACATCCCCGTTGG AAACCTGCCAGAAGACATCTCCACCTTTGGCTCTGACCTCTTTTTTGCACGCCATCTCCAGCGCCATAAC CACCTACTTTGGTTGTCCCCTACCTCTCGGCCTGACCTGGGTGGGAAGGAAGCTGATGACAACCGCCTTG TCATGGAGTTTGATGACCGAGCCACTGTGGAGATCAATAATTCCGGCTGTTACTCTACTGTGTGCGTGGA GCTGGACATTCAAAACCTGGCAGTCAACACCATCCTCCAGTCCCACCATGTCAATGACATGGAGGGGGCT GGCAGCATGGGCATCAGCTTCGATGTGATCCAGCAGGCCTCCCTAGAGGACATGGTTACAGGCAATCAAG CTGCCAGTGCCCTGGCCAGCTACGATGAGACAGCCCTCTGCTCCAGTACGTTCAGGATCCTGAAGAGCAT GGTGGTTGGCTGGGTAAAGGAAATCACACAGTACCACAACATCTATGCTGACAACCAGGTGATGCACTTC TACCGCTGGCTCCAGTCGCCGTGCTCTCTGCTCCACGACCCAGCCCTTCACCGGACACTGCACAATATGA TGAAGAAGCTCTTCCTGCAGCTCATTGCTGAGTTCAAGCGCCTGGGGTCATCGGTCATCTATGCCAACTT CAATCGCATCATCCTCTGTACAAAGAAGCGCCGGATAGAGGATGCCCTTGCCTATGTGGAATATATCACC AACAGCATCCATTCTAAAGAGATCTTTCATTCCCTGACCATCTCTTTCTCTCGATGCTGGGAATTCCTTC TCTGGATGGATCCATCCAACTATGGTGGAATCAAAGGAAAAGTCCCATCCAGTATTCACTGTGGACAAGT AAGGGAGCAAGACTCCCAGACAAGGGAGGAAACTGAGGATGAGGAGGACAATGAAAAGGATGAGGAGGAA GAGGAGGACATGGGGCAATCTGAGGTTGAGGACTTACTGGAGAACAACTGGAACATTCTACAGTTCTTGC CACAGGCAGCCTCTTGCCAGAGCTACTTCCTTATGATCGTTTCAGCATACATCGTGGCTGTGTACCAAAG CATGAAGGATGAGTTGAGACACAGTGCCCCAGGCAGTACCCCTGTGAAGAGGAAGGGGGCCAGCCAGTTC TCCCAGGAGGCTGAAGGGGCAGCCGGGGCCCTTCCTGGAATGATCACTTTCTCTCAAGATTATGTGGCAA ATGAGCTCACTCAGACCTTCTTCACCATTACTCAGAAAATTCAGAAGAAAGTCACAGGCTCTCGGAACAC CGCTGAGCCCTCAGAGATGTTCCCTGTCCTCCCTGGTTCACATTTGCTGCTCAATAACCCTGCTCTGGAG TTCATCAAATACGTGTGCAAGGTACTATCTCTGGATACAAACATCACAAATCAGGTGAATAAGCTAAACA GAGACCTGCTTCGCCTGGTAGACGTTGGTGAATTCTCTGAGGAGGCCCAGTTCAGAGACCCCTGCCACTC CTACGTGCTCCCTGAGGTAATCTGCCACAGCTGTAATTTCTGCCGAGACCTGGACCTATGCAAAGATTCT TCTTTCTCTCAGGACGGAGCCATCCTGCCTCAGTGGCTTTGCTCCAATTGTCAGGCCCCCTACGACTCAT CTGCCATTGAGTCAGCCTTGGTGGAAGCCCTGCAGAGGAAACTGATGGCCTTCACACTTCAGGACCTGGT GTGCCTCAAGTGCCGTGGTATGAAAGAGACCCATATGCCTGTGTACTGCGGCTGCGCAGGGGACTTTGTT CTCACCATCCATACTGAGGTCTTCATGGAACAGATTAGAATCTTCCAGAACATTGCCAAGTACTACAACA TGTCATATCTCCAAGAGGCCATAGAATGGCTGCTACAGACAAGCCCTGTATCAAACTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107152
Insert Size	6852 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107152.2, NP_001100622.2
RefSeq Size	7101 bp
RefSeq ORF	6852 bp
Locus ID	304573

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.