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Alox12b (NM_001039377) Rat Untagged Clone
CAT＃: RN211780
Alox12b (untagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug)
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN211780 representing NM_001039377
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCTACAAAGTCAAGGTGGCCACGGGCACTGACTTCTTTTCGGGGACGCTGGACTCAATTTCCC TGACCATCGTGGGCACCCAAGGAGAGAGCCATAAGCAGCGGTTGAACCACTTTGGGAGAGACTTTGCCAC AGGGGCCGTGGATGACTACACAGTGCAGTGCCAGCAGGACCTGGGGGAGCTCATCATCATCCGTCTGCAC AAGGAGCCTCATTCCTTCTTACCCAAGGACCCCTGGTACTGCAACTATGTGCAGATCTGCGCTCCCAACT GTCGTGTCTACCACTTCCCAGCTTACCAGTGGATGGATGGCTATGAGACCCTGTCACTCCGGGAGGCCAC AGGAAAGACAACAGCAGATGACACACTTCCCATCCTTTTGGAACACAGACAAGAAGAGATCAGAGCCAAG AAAGACTTCTACCACTGGAGGGTCTTTGTTCCCGGCCTTCCCAACTATGTGGACATCCCCAGTTACCATC CTCCTCCCAGACGGTGCCGCAACCCCAACCGGCCTGAGTGGAATGGTTATATTCCAGGATTCCCAATTCT CATCAACATTAAGGCCACCAGGTTCTTAAACTTAAATCTTCGTTTCTCCTTCGTCAAGACAGCCTCCTTC TTCTACCGCCTAGGGCCCATGGCACTGGCCTTCAAGCTTCGTGGCCTGGTGGATCGCAAACGCTCTTGGA AGAGACTAAAGGACATTAAGAACATTTTCCCTGCTACCAAGACTGTCGTCTCTGAGTATGTGGCCGAGCA CTGGACTGAAGATAGCTTCTTTGGGTACCAGTACCTCAACGGCATCAACCCAGGCCATATCCGAAGATGC ATGCAGATCCCAGACAAGTTCCCAGTTACAGATGAAATGGTGGCCCCATTCCTGGGTGAAGGAACATGCC TGCAGGCAGAGCTGGAGAAGGGGAATATCTACCTGGCAGACTACCGCATCTTGGATGGCATCCCCACGGT GGAGCTCAATGGTCAGAAGCAGCATCACTGTGCCCCGATCTGCTTGCTGCACTTTGGTCCCGATGGTAAC ATGATGCCTATTGCCATTCAGCTCAGCCAGACTCCTGGGCCTGATTGTCCCATCTTCCTGCCTAATGATT CTGAGTGGGACTGGCTGTTGGCTAAAACATGGGTACGCTACGCTGAGTTCTACAGCCACGAGGCCGTTGC CCACTTACTGGAGAGTCACCTCATCGGGGAAGCTTTCTGCTTGGCCCTCCTGAGGAACCTGCCCATGTGC CACCCCCTGTATAAGCTCCTCATTCCTCACACTCGCTACAACGTCCAGATCAACAGCATTGGGCGGGCCC TCCTTCTCAACAAAGGGGGTCTCTCTGCCAGGGCCATGTCCCTGGGCCTAGAGGGCTTCGCACAGGTGAT GGTTCGGGGTCTGTCTGAGCTCACCTACAAAAGCCTCTGCATCCCCAATGACTTTGTGGAGCGCGGGGTT CAGGATCTGCCTGGGTATTATTTCCGTGACGACAGCTTGGCGGTGTGGTATGCCATGGAGAGGTACGTGA CAGAGATCATCACTTATTATTACCCGAATGACGCAGCTGTGGAGGGCGACCCGGAATTGCAGTGCTGGGT GCAGGAGATATTCAAGGAGTGTCTCCTGGAGCGTGAGAGCTCAGGTTTTCCCACCTGCTTGCGAACTGTA CCTGAGCTGATCGAATACGTCACGATGGTCATGTACACCTGCTCAGCGAGGCATGCAGCTGTCAACACAG GCCAGTTGGAATACACTTCTTGGATGCCCAACTTCCCGTCATCCATGAGGAACCCGCCGATGCAGTCCAA GGGGCTGACTACTTTGCAGACATTCATGGACACTTTGCCAGACGTGAAGACCACATGCATTGTGCTCTTG GTGCTCTGGACTCTCTGCCGGGAGCCTGATGACAGGCGGCCACTTGGCCACTTCCCGGACATCCATTTTG TGGAGGAGGCCCCTCGGAGGAGTATGGAGGCTTTTCGTCAGAACCTCAACCAGATCTCTCACAACATCCG CCAGCGCAATAAGTGCCTCAACCTCCCGTATTACTACCTGGACCCGGTGCTGATTGAGAACAGTATTTCC ATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001039377 |
| Insert Size | 2106 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001039377.1, NP_001034466.1 |
| RefSeq Size | 2268 bp |
| RefSeq ORF | 2106 bp |
| Locus ID | 287425 |
| UniProt ID | Q2KMM4 |
| Gene Summary | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR211780 | Alox12b (Myc-DDK-tagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug) |
CNY 9,030.00 |
|
| RR211780L3 | Lenti ORF clone of Alox12b (Myc-DDK-tagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug) |
CNY 10,830.00 |
|
| RR211780L4 | Lenti ORF clone of Alox12b (mGFP-tagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug) |
CNY 11,880.00 |
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