Abcb11 (NM_031760) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Bsep; Spgp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211590 representing NM_031760 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACTCAGTGATTCTTCGCAGCGTGAAGAAATTTGGAGAGGAGAATCATGCTTTCGAATCAGATG GATCACATAACAATGATAAGAAATCAAGGTTACAAGATAAGATGAAGGAGGGAGACATTCGAGTTGGCTT CTTTGAACTGTTTCGATTTTCTTCATCAAAAGACATCTGGCTGATGCTTATGGGAGGCGTATGCGCATTG CTACATGGAATGGCCCAGCCAGGCATACTTATTATTTTTGGTATAATGACAGATATTTTTATTAAATATG ACATTGAAAGGCAAGAACTCGAGATACCAGGAAAAGCGTGTGTGAATAACACCATTGTATGGATCAACAG CTCCTTCCACCAGAACATGACAAACGGAACAGTCTGTGGGTTGGTGGACATTGAAAGTGAAATGATCAAA TTCTCTGGCATCTACGCAGGAGTCGGCATGACCGTACTTATCCTTGGATACTTTCAAATAAGGTTATGGG TCATCACTGGGGCTCGTCAGATAAGGAGAATGAGGAAAATTTACTTCCGGAGAATAATGAGAATGGAAAT TGGGTGGTTCGACTGCACTTCTGTGGGAGAGCTGAATTCAAGGTTTGCTGATGATATTGAAAAAATCAAT GACGCCATTGCCGACCAGTTGGCTCATTTCCTCCAGCGCATGTCGACGGCTATGTGTGGGTTACTTTTAG GGTTCTACAGGGGTTGGAAACTAACCTTGGTGATTCTTGCTGTCAGCCCTCTCATTGGCATTGGGGCAGC CGTCATAGGTCTGAGTATAGCCAAGTTCACGGAGCTTGAGTTGAAGGCTTATGCCAAAGCGGGGTCTATT GCTGATGAAGTCCTCTCATCTATTCGAACAGTGGCCGCTTTTGGTGGTGAGAACAAAGAGGTTGAAAGGT ATGAGAAAAATCTTGTGTTTGCCCAGCGCTGGGGAATTTGGAAAGGAATGGTGATGGGCTTCTTCACTGG GTACATGTGGTGTCTCATTTTCTTCTGTTATGCACTGGCCTTCTGGTATGGTTCCACACTTGTCCTAGAT GAAGAAGAGTATACACCAGGGACACTGGTCCAGATTTTCCTCTGTGTCATATTAGCAGCTATGAATATTG GCCATGCATCTTCTTGCTTGGAAATCTTCTCCACTGGGTGTTCAGCAGCTACCAATATTTTTCAAACAAT AGACAGGCAACCTGTCATTGACTGCATGTCAGGAGACGGCTACAAGCTAGACCGAATCAAGGGTGAAATT GAGTTCCACAATGTGACCTTCCATTATCCTTCTAGACCGGACGTGAAGATTTTAGATAACCTCAGCATGG TCATAAAGCCAGGGGAAACGACGGCTCTGGTGGGATCCAGTGGGGCTGGGAAGAGTACAGCATTACAGCT CATTCAGAGATTCTATGACCCCTGTGAAGGCATGGTGACTCTGGACGGCCATGACATTCGCTCTCTTAAC ATCCGGTGGCTGAGAGATCAAATCGGGATCGTGGAACAGGAGCCCGTTCTGTTCTCCACCACTATCGCAG AAAACATCCGTTTTGGCAGAGAAGATGCAACAATGGAAGACATAGTCCAAGCTGCCAAGGATGCTAATGC ATACAACTTCATTATGGCCCTGCCGCAGCAATTTGACACCCTTGTTGGAGAAGGAGGAGGCCAGATGAGT GGTGGTCAGAAGCAAAGAGTAGCCATTGCCCGAGCCCTCATACGGAATCCCAAGATCTTGCTTCTGGATA TGGCTACCTCAGCACTGGACAATGAGAGTGAAGCTAGAGTACAAGAAGCATTGAATAAGATCCAACATGG GCATACAATCATCTCAGTTGCCCATCGCCTGTCAACAGTCAGAGCTGCAGATGTGATCATTGGGTTTGAG CATGGAGTAGCTGTGGAAAGAGGCACCCATGAAGAGCTGCTAGAAAGAAAAGGTGTCTACTTCATGCTTG TGACCCTGCAAAGCCAAGGAGATAATGCTCACAAAGAAACGAGCATAATGGGGAAAGATGCGACGGAAGG TGGCACCCTTGAGAGGACCTTTTCCAGAGGCAGCTATCGGGATAGTTTAAGAGCTTCGATCCGGCAACGC TCCAAGTCTCAGCTGTCTCTTCTGACACATGACCCTCCACTGGCTGTTGCTGATCACAAATCCTCTTACA AAGACAGCAAGGACAATGACGTGCTTGTGGAAGAAGTTGAACCTGCCCCAGTTAGGAGGATTCTAAAATA CAACATTCCAGAATGGCACTACATTCTGGTAGGATCTTTGAGTGCAGCCATTAATGGGGCAGTCACACCC ATCTACTCCCTTTTATTCAGCCAGCTCCTTGGGACTTTTTCACTCCTCGATAAAGAACAACAAAGGTCAG AGATTCACAGCATGTGTCTGTTCTTTGTCATCCTGGGCTGTGTATCCATTTTCACACAATTTCTGCAGGG TTACACTTTTGCCAAATCCGGAGAGCTCCTCACAAAGAGGCTGCGGAAATTTGGTTTCAAGGCAATGTTA GGACAAGATATCGGCTGGTTCGATGACCTCAGAAATAATCCTGGAGTACTGACGACTAGGCTTGCTACAG ATGCTTCCCAAGTTCAAGGGGCTACTGGCTCTCAAGTTGGAATGATGGTCAATTCCTTCACTAACATCAT TGCGGCCTTGCTGATTGCCTTCTTCTTTAGCTGGAAGCTCAGTCTGATTATAACGATCTTCTTCCCCTTT CTGGCTTTATCGGGAGCTGTACAGACAAAAATGTTGACGGGATTCGCTTCTCAAGACAAGCAAGCTCTGG AGAAGGCTGGTCAGATCACCAGCGAAGCCCTCAGCAATATCCGCACAGTTGCTGGGATTGGAGTGGAGGG AAGATTTATTAAAGCATTTGAGGTTGAGCTCCAGACATCATACAAGACTGCTGTCAGGAAGGCGAATATC TATGGACTCTGCTTTGCCTTTTCCCAGGGGATAGCATTTCTTGCAAATTCCGCTGCCTATAGATATGGAG GTTACTTAATAGCCTACGAAGGTCTGGGCTTCAGCCACGTTTTCAGGGTGGTCTCTTCAGTTGCATTGAG TGCAACAGCCGTTGGAAGAACATTCTCTTATACTCCGAGCTATGCCAAAGCTAAAATATCAGCTGCACGC TTTTTTCAACTGCTAGATCGGAAACCTCCAATTAATGTGTACAGTGAAGCAGGTGAAAAATGGGACAACT TCCAAGGGAAGATTGATTTTATTGACTGTAAATTTACGTATCCTTCTCGACCCGATATTCAAGTTCTGAA TGGTCTCTCAGTATCTGTTAATCCTGGGCAGACGCTGGCATTTGTTGGAAGCAGTGGGTGTGGCAAAAGC ACCAGCATTCAGCTGTTGGAACGGTTCTATGATCCCGATCAGGGAACTGTGATGATAGATGGTCATGACA GCAAAAAAGTCAACATTCAGTTCCTCCGTTCCAACATCGGGATTGTCTCCCAGGAGCCAGTGCTGTTTGA CTGTAGCATAATGGACAACATCAAGTACGGGGACAACACTAAAGAGATCTCCGTGGAGAGAGCCATAGCT GCTGCAAAGCAGGCTCAGCTGCATGACTTCGTCATGTCGCTCCCAGAGAAATATGAAACTAATGTTGGGA TCCAGGGCTCTCAGCTCTCTCGTGGAGAGAAACAACGCATTGCTATTGCTCGGGCCATTGTGCGAGATCC TAAAATCTTACTACTGGATGAAGCTACGTCTGCCCTAGACACAGAAAGTGAAAAGACAGTGCAGACTGCT CTGGACAAAGCCAGAGAGGGTCGGACCTGCATTGTCATTGCTCATCGTTTGTCCACCATCCAGAACTCAG ATATCATTGCTGTCGTGTCACAAGGAGTGGTGATTGAAAAAGGGACCCATGAGAAACTGATGGCCCAGAA GGGAGCCTACTACAAGCTGGTCATCACTGGAGCCCCCATCAGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_031760
Insert Size	3966 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031760.1, NP_113948.1
RefSeq Size	5036 bp
RefSeq ORF	3966 bp
Locus ID	83569
UniProt ID	O70127
Gene Summary	acts as an ATP-dependent bile salt export pump; transports taurochenodeoxycholate, taurocholate, and other bile salts [RGD, Feb 2006]

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