Scn10a (NM_017247) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Na(V)1.8; Nav1.8; PN3
Vector	pCMV6-AC-Myc-DDK
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211430 representing NM_017247 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCCCCTTTGCGTCCGTGGGAACTACCAATTTCAGACGGTTCACTCCAGAGTCACTGGCAGAGA TCGAGAAGCAGATTGCTGCTCACCGCGCAGCCAAGAAGGCCAGAACCAAGCACAGAGGACAGGAGGACAA GGGCGAGAAGCCCAGGCCTCAGCTGGACTTGAAAGACTGTAACCAGCTGCCCAAGTTCTATGGTGAGCTC CCAGCAGAACTGGTCGGGGAGCCCCTGGAGGACCTAGACCCTTTCTACAGCACACACCGGACATTCATGG TGTTGAATAAAAGCAGGACCATTTCCAGATTCAGTGCCACTTGGGCCCTGTGGCTCTTCAGTCCCTTCAA CCTGATCAGAAGAACAGCCATCAAAGTGTCTGTCCATTCCTGGTTCTCCATATTCATCACCATCACTATT TTGGTCAACTGCGTGTGCATGACCCGAACTGATCTTCCAGAGAAAGTCGAGTACGTCTTCACTGTCATTT ACACCTTCGAGGCTCTGATTAAGATACTGGCAAGAGGGTTTTGTCTAAATGAGTTCACTTATCTTCGAGA TCCGTGGAACTGGCTGGACTTCAGTGTCATTACCTTGGCGTATGTGGGTGCAGCGATAGACCTCCGAGGA ATCTCAGGCCTGCGGACATTCCGAGTTCTCAGAGCCCTGAAAACTGTTTCTGTGATCCCAGGACTGAAGG TCATCGTGGGAGCCCTGATCCACTCAGTGAGGAAGCTGGCCGACGTGACTATCCTCACAGTCTTCTGCCT GAGCGTCTTCGCCTTGGTGGGCCTGCAGCTCTTTAAGGGGAACCTTAAGAACAAATGCATCAGGAACGGA ACAGATCCCCACAAGGCTGACAACCTCTCATCTGAAATGGCAGAATACATCTTCATCAAGCCTGGTACTA CGGATCCCTTACTGTGCGGCAATGGGTCTGATGCTGGTCACTGCCCTGGAGGCTATGTCTGCCTGAAAAC TCCTGACAACCCGGATTTTAACTACACCAGCTTTGATTCCTTTGCGTGGGCATTCCTCTCACTGTTCCGC CTCATGACGCAGGACTCCTGGGAGCGCCTGTACCAGCAGACACTCCGGGCTTCTGGGAAAATGTACATGG TCTTTTTCGTGCTGGTTATTTTCCTTGGATCGTTCTACCTGGTCAATTTGATCTTGGCCGTGGTCACCAT GGCGTATGAAGAGCAGAGCCAGGCAACAATTGCAGAAATCGAAGCCAAGGAAAAAAAGTTCCAGGAAGCC CTTGAGGTGCTGCAGAAGGAACAGGAGGTGCTGGAAGCCCTGGGGATTGACACGACCTCGCTCCAGTCCC ACAGTGGATCACCCTTAGCCTCCAAAAACGCCAATGAGAGAAGACCCAGGGTGAAATCAAGGGTGTCAGA GGGCTCCACGGATGACAACAGGTCACCCCAATCTGACCCTTACAACCAGCGCAGGATGTCTTTCCTAGGC CTGTCTTCAGGAAGACGCAGGGCTAGCCACGGCAGTGTGTTCCACTTCCGAGCGCCCAGCCAAGACATCT CATTTCCTGACGGGATCACCCCTGATGATGGGGTCTTTCACGGAGACCAGGAAAGCCGTCGAGGTTCCAT ATTGCTGGGCAGGGGTGCTGGGCAGACAGGTCCACTCCCCAGGAGCCCACTGCCTCAGTCCCCCAACCCT GGCCGTAGACATGGAGAAGAGGGACAGCTCGGAGTGCCCACTGGTGAGCTTACCGCTGGAGCGCCTGAAG GCCCGGCACTGCACACTACAGGGCAGAAGAGCTTCCTGTCTGCGGGCTACTTGAACGAACCTTTCCGAGC ACAGAGGGCCATGAGCGTTGTCAGTATCATGACTTCTGTCATTGAGGAGCTTGAAGAGTCTAAGCTGAAG TGCCCACCCTGCTTGATCAGCTTCGCTCAGAAGTATCTGATCTGGGAGTGCTGCCCCAAGTGGAGGAAGT TCAAGATGGCGCTGTTCGAGCTGGTGACTGACCCCTTCGCAGAGCTTACCATCACCCTCTGCATCGTGGT GAACACCGTCTTCATGGCCATGGAGCACTACCCCATGACCGATGCCTTCGATGCCATGCTTCAAGCCGGC AACATTGTCTTCACCGTGTTTTTCACAATGGAGATGGCCTTCAAGATCATTGCCTTCGACCCCTACTATT ACTTCCAGAAGAAGTGGAATATCTTCGACTGTGTCATCGTCACCGTGAGCCTTCTGGAGCTGAGTGCATC CAAGAAGGGCAGCCTGTCTGTGCTCCGTACCTTACGCTTGCTGCGGGTCTTCAAGCTGGCCAAGTCCTGG CCCACCCTGAACACCCTCATCAAGATCATCGGGAACTCAGTGGGGGCCCTGGGCAACCTGACCTTTATCC TGGCCATCATCGTCTTCATCTTCGCCCTGGTCGGAAAGCAGCTTCTCTCAGAGGACTACGGGTGCCGCAA GGACGGCGTCTCCGTGTGGAACGGCGAGAAGCTCCGCTGGCACATGTGTGACTTCTTCCATTCCTTCCTG GTCGTCTTCCGAATCCTCTGCGGGGAGTGGATCGAGAACATGTGGGTCTGCATGGAGGTCAGCCAGAAAT CCATCTGCCTCATCCTCTTCTTGACTGTGATGGTGCTGGGCAACCTAGTGGTGCTCAACCTTTTCATCGC TTTACTGCTGAACTCCTTCAGCGCGGACAACCTCACGGCTCCAGAGGATGACGGGGAGGTGAACAACTTG CAGTTAGCACTGGCCAGGATCCAGGTACTTGGCCATCGGGCCAGCAGGGCCATCGCCAGTTACATCAGCA GCCACTGCCGATTCCACTGGCCCAAGGTGGAGACCCAGCTGGGCATGAAGCCCCCACTCACCAGCTCAGA GGCCAAGAACCACATTGCCACTGATGCTGTCAGTGCTGCAGTGGGGAACCTGACAAAGCCAGCTCTCAGT AGCCCCAAGGAGAACCACGGGGACTTCATCACTGATCCCAACGTGTGGGTCTCTGTGCCCATTGCTGAGG GGGAATCTGACCTCGACGAGCTCGAGGAAGATATGGAGCAGGCTTCGCAGAGCTCCTGGCAGGAAGAGGA CCCCAAGGGACAGCAGGAGCAGTTGCCACAAGTCCAAAAGTGTGAAAACCACCAGGCAGCCAGAAGCCCA GCCTCCATGATGTCCTCTGAGGACCTGGCTCCATACCTGGGTGAGAGCTGGAAGAGGAAGGATAGCCCTC AGGTCCCTGCCGAGGGAGTGGATGACACGAGCTCCTCTGAGGGCAGCACGGTGGACTGCCCGGACCCAGA GGAAATCCTGAGGAAGATCCCCGAGCTGGCAGATGACCTGGACGAGCCCGATGACTGTTTCACAGAAGGC TGCACTCGCCGCTGTCCCTGCTGCAACGTGAATACTAGCAAGTCTCCTTGGGCCACAGGCTGGCAGGTGC GCAAGACCTGCTACCGCATCGTGGAGCACAGCTGGTTTGAGAGTTTCATCATCTTCATGATCCTGCTCAG CAGTGGAGCGCTGGCCTTTGAGGATAACTACCTGGAAGAGAAACCCCGAGTGAAGTCCGTGCTGGAGTAC ACTGACCGAGTGTTCACCTTCATCTTCGTCTTTGAGATGCTGCTCAAGTGGGTAGCCTATGGCTTCAAAA AGTATTTCACCAATGCCTGGTGCTGGCTGGACTTCCTCATTGTGAACATCTCCCTGACAAGCCTCATAGC GAAGATCCTTGAGTATTCCGACGTGGCGTCCATCAAAGCCCTTCGGACTCTCCGTGCCCTCCGACCGCTG CGGGCTCTGTCTCGATTCGAAGGCATGAGGGTAGTGGTGGATGCCCTCGTGGGCGCCATCCCCTCCATCA TGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGCGTGAACCTCTTCGCCGG GAAATTTTCGAAGTGCGTCGACACCAGAAATAACCCATTTTCCAACGTGAATTCGACGATGGTGAATAAC AAGTCCGAGTGTCACAATCAAAACAGCACCGGCCACTTCTTCTGGGTCAACGTCAAAGTCAACTTCGACA ACGTCGCTATGGGCTACCTCGCACTTCTTCAGGTGGCAACCTTCAAAGGCTGGATGGACATAATGTATGC AGCTGTTGATTCCGGAGAGATCAACAGTCAGCCTAACTGGGAGAACAACTTGTACATGTACCTGTACTTC GTCGTTTTCATCATTTTCGGTGGCTTCTTCACGCTGAATCTCTTTGTTGGGGTCATAATCGACAACTTCA ACCAACAGAAAAAAAAGCTAGGAGGCCAGGACATCTTCATGACAGAAGAGCAGAAGAAGTACTACAATGC CATGAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCCCTGAATAAGTACCAAGGCTTC GTGTTTGACATCGTGACCAGGCAAGCCTTTGACATCATCATCATGGTTCTCATCTGCCTCAACATGATCA CCATGATGGTGGAGACCGACGAGCAGGGCGAGGAGAAGACGAAGGTTCTGGGCAGAATCAACCAGTTCTT TGTGGCCGTCTTCACGGGCGAGTGTGTGATGAAGATGTTCGCCCTGCGACAGTACTACTTCACCAACGGC TGGAACGTGTTCGACTTCATAGTGGTGATCCTGTCCATTGGGAGTCTGCTGTTTTCTGCAATCCTTAAGT CACTGGAAAACTACTTCTCCCCGACGCTCTTCCGGGTCATCCGTCTGGCCAGGATCGGCCGCATCCTCAG GCTGATCCGAGCAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCCCGCCCTCTTC AACATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAGCTTCGCTAACG TCGTGGACGAGGCCGGCATCGACGACATGTTCAACTTCAAGACCTTTGGCAACAGCATGCTGTGCCTGTT CCAGATCACCACCTCGGCCGGCTGGGACGGCCTCCTCAGCCCCATCCTCAACACGGGGCCTCCCTACTGC GACCCCAACCTGCCCAACAGCAACGGCTCCCGGGGGAACTGCGGGAGCCCGGCGGTGGGCATCATCTTCT TCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATCGCAGTGATTCTGGAGAACTT CAACGTAGCCACCGAGGAGAGCACGGAGCCCCTGAGCGAGGACGACTTCGACATGTTCTATGAGACCTGG GAGAAGTTCGACCCGGAGGCCACCCAGTTCATTGCCTTTTCTGCCCTCTCAGACTTCGCGGACACGCTCT CCGGCCCTCTTAGAATCCCCAAACCCAACCAGAATATATTAATCCAGATGGACCTGCCGTTGGTCCCCGG GGATAAGATCCACTGTCTGGACATCCTTTTTGCCTTCACAAAGAACGTCTTGGGAGAATCCGGGGAGTTG GACTCCCTGAAGACCAATATGGAAGAGAAGTTTATGGCGACCAATCTCTCCAAAGCATCCTATGAACCAA TAGCCACCACCCTCCGGTGGAAGCAGGAAGACCTCTCAGCCACAGTCATTCAAAAGGCCTACCGGAGCTA CATGCTGCACCGCTCCTTGACACTCTCCAACACCCTGCATGTGCCCAGGGCTGAGGAGGATGGCGTGTCA CTTCCCGGGGAAGGCTACATTACATTCATGGCAAACAGTGGACTCCCGGACAAATCAGAAACTGCCTCTG CTACGTCTTTCCCGCCATCCTATGACAGTGTCACCAGGGGCCTGAGTGACCGGGCCAACATTAACCCATC TAGCTCAATGCAAAATGAAGATGAGGTCGCTGCTAAGGAAGGAAACAGCCCTGGACCTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_017247
Insert Size	5874 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017247.1, NP_058943.1
RefSeq Size	6524 bp
RefSeq ORF	5874 bp
Locus ID	29571
UniProt ID	Q62968
Gene Summary	voltage-gated sodium channel that is resistant to tetrodotoxin [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.