United States
Germany
Japan
United Kingdom
China
Wrap53 (NM_001007610) Rat Untagged Clone
CAT＃: RN211362
Wrap53 (untagged ORF) - Rat WD repeat domain 79 (Wdr79), (10 ug)
CNY 6,840.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Wdr79 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN211362 representing NM_001007610
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACGTCGGAGGAGCTACGCCTAGCTCCGGATAGCCTCCCTTCTGACCTGGTCCCAGCCCCAGTGC TCCAAGCTTCCCCCGCGGACAAAAATACAGACTCTGAACCGGTGCCTCCGCCATGCGGTGGAGATGACCA ACTCCAGGTAGCCACAGATGCCGTGGCCAGCTCGGTTGTGTCCCAGGAGCTTCAACAAGGGGACTCAGCT CCTCTGGAAGTAGAATTTAATATTTCCAGTGAGTTGAGTCCTCGAATCGAGGAGCAAGAAGTTCCTGAAA ATGCCAGCCTTCCTGTAGAAGAAACGAATCGGCCCGAGTTGGAGTCAGGGGAAGCCATGGAAGGTGTGTC TGAGGAACCCGCTGCAGTGGATGAGGGGGACATCTTTTGGAGCTACAGCTTCTCTCAGGTACCCCAATAC CTCAGTGGCTCCTGGTCCGAGTTCAGCACCCGCTCCGAGAACTTCTTGAAAGGTTGCAAGTGGGCCCCTG ACGGTTCCTGTATCTTGACCAATAGTGCTGACAATGTCCTACGGATTTACAACCTGCCCCCAGAACTGTA CAGTGAAAGCGAGCAGGTGGATTATGCCGAAATGGTTCCTGTCCTGCGAATGGTAGAGGGTGACACCATC TATGATTACTGCTGGTATTCTCTGATGTCCTCCACCCAGCCAGACACCTCCTATGTGGCCAGCAGCAGCC GGGAGAACCCAATTCACATCTGGGATGCATTCACTGGAGAACTCCGGGCTTCCTTTCGCGCTTACAACCA CCTGGACGAGTTGACAGCGGCCCACTCACTCTGTTTCTCCCCGGATGGTTCCCAACTCTTTTGTGGCTTC AACCGGACTGTGCGTGTCTTTTCCACATCCAGGCCTGGCAGAGACTGTGAGGTGCGAACCACATTTGCCA AAAAGCAGGGCCAGAGTGGCATCATCTCCTGTCTAGCCTTTAGTCCAGCCCAGCCCCTCTATGCCTGTGG CTCCTATGGCCGCACCCTGGGCCTGTATGCCTGGGATGATGGTTCACCTCTTGCCTTGCTGGGGGGCCAT CAAGGAGGTATCACCCACCTTTGCTTTCACCCTGATGGCAACCTCTTCTTCTCAGGAGCCCGAAAGGATG CTGAACTTCTGTGCTGGGATCTCCGGCAGCCGGGCCACCTCCTGTGGTCTCTGAGTCGGGAAGTGACCAC CAATCAGCGCATCTACTTTGATCTGGACCCGAGCGGGCAGTTCCTAGTGAGTGGCAACACCAGTGGAGTT GTCTCCGTGTGGGACATCAGTGGAGCGTTCAGTGACTGCAAGCAGCTGGAGCCAGTGATGACTTTTCTGC CCCAGGACGACTGCACCAATGGAGTCAGCCTTCACCCTACCTTGCCTCTGCTGGCCACTGCTTCTGGCCA GCGCATGTTTCCAGAGCCCACCAATAGCGGGGATGAAGGTGAGCCGGAGCTGGACCTTCCCCTGCTCTCC CTGCGCCACGCCCATCCTGAATGTCAGCTACAGCTCTGGTGGTGTGGAGGGGGACCTGACCCCAGTAACC CTGATGAGGACCAGGATGAGAAGGGACAAGGAAGGACAGAGGCTGTCGGAATGAGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001007610 |
| Insert Size | 1599 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001007610.1, NP_001007611.1 |
| RefSeq Size | 1782 bp |
| RefSeq ORF | 1599 bp |
| Locus ID | 287432 |
| UniProt ID | Q5XII5 |
| Gene Summary | RNA chaperone that plays a key role in telomere maintenance and RNA localization to Cajal bodies. Specifically recognizes and binds the Cajal body box (CAB box) present in both small Cajal body RNAs (scaRNAs) and telomerase RNA template component (TERC). Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, required to stimulate the catalytic activity of the complex. Acts by specifically binding the CAB box of the TERC RNA and controlling the folding of the CR4/CR5 region of the TERC RNA, a critical step for telomerase activity. In addition, also controls telomerase holoenzyme complex localization to Cajal body. During S phase, required for delivery of TERC to telomeres during S phase and for telomerase activity. In addition to its role in telomere maintenance, also required for Cajal body formation, probably by mediating localization of scaRNAs to Cajal bodies. Also plays a role in DNA repair: phosphorylated by ATM in response to DNA damage and relocalizes to sites of DNA double-strand breaks to promote the repair of DNA double-strand breaks. Acts by recruiting the ubiquitin ligase RNF8 to DNA breaks and promote both homologous recombination (HR) and non-homologous end joining (NHEJ).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR211362 | Wrap53 (Myc-DDK-tagged ORF) - Rat WD repeat domain 79 (Wdr79), (10 ug) |
CNY 6,840.00 |
|
| RR211362L3 | Lenti ORF clone of Wrap53 (Myc-DDK-tagged ORF) - Rat WD repeat domain 79 (Wdr79), (10 ug) |
CNY 8,740.00 |
|
| RR211362L4 | Lenti ORF clone of Wrap53 (mGFP-tagged ORF) - Rat WD repeat domain 79 (Wdr79), (10 ug) |
CNY 9,600.00 |
