Abcb1b (NM_012623) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Abcb1; Mdr1; mdr1b; Pgy1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211334 representing NM_012623 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTTGAAGAGGGCCTTAACGGAACAGCAGACAAGAACTTCTCAAAGATGGGCAAAAAGAGTAAAA AGGAGAAGGAGAAGAAACCTGCTGTTGGCATATTCGGGATGTTTCGCTATGCAGATTGGCTTGACAAGCT GTGCATGGCTCTGGGAACTCTCGCTGCTATCATCCACGGAACCCTGCTTCCCCTCCTGATGCTGGTGTTC GGATACATGACAGATAGTTTTACCCAAGCAGAGACCCGCATTCTGCCGAGCGTTACTAATCAAAGTGAAA TCAACAGTACACAGACCGTCAGCGACAGCAGTCTGGAGGAGGACATGGCCATGTACGCCTACTATTACAC GGGCATTGGTGCCGGTGTGCTCATCGTTGCCTACATCCAGGTTTCACTTTGGTGCCTGGCAGCTGGGAGA CAAATACACAAGATTAGGCAGAAGTTTTTCCATGCCATCATGAATCAGGAGATAGGCTGGTTTGACGTGA ATGACGCTGGGGAGCTCAACACCCGGCTCACAGATGACGTCTCCAAAATTAATGACGGAATTGGTGACAA ACTTGGAATGTTCTTTCAGTCCATAACGACATTTTCAGCCGGTTTTATAATAGGATTTATAAGTGGTTGG AAGCTAACCCTTGTAATTTTGGCCGTCAGCCCTCTTATTGGGTTGTCATCTGCCATGTGGGCAAAGGTAC TGACTTCATTTACTAATAAGGAACTCCAGGCTTATGCGAAAGCTGGAGCAGTTGCCGAAGAAGTCTTAGC AGCCATCAGAACTGTGATTGCATTTGGAGGACAAAAGAAGGAACTTGAAAGGTACAATAAAAATTTAGAA GAAGCTAAAAGAGTTGGCATAAAGAAAGCCATCACGGCCAACATTTCCATAGGTATTGCCTACCTGTTGG TCTATGCGTCTTATGCACTGGCATTCTGGTATGGGACCTCCTTGGTCCTCTCAAATGAATATTCTATTGG ACAAGTGCTTACCGTCTTCTTCTCTATTTTATTGGGGACTTTCAGTATTGGACATTTAGCCCCAAACATA GAAGCCTTTGCAAATGCAAGAGGGGCAGCCTATGAAATCTTCAAGATAATTGATAATGAGCCAAGCATTG ACAGCTTCTCAACCAAGGGACACAAACCAGACAGTATAATGGGAAATTTGGAATTTAAAAATGTTTACTT CAACTACCCATCACGAAGTGAAGTTAAGATCTTGAAGGGCCTCAACCTGAAGGTGAAGAGCGGGCAGACG GTAGCCCTGGTTGGCAACAGTGGCTGTGGGAAAAGCACAACTGTCCAGCTGCTGCAGAGGCTCTACGACC CCATAGAGGGCGAGGTCAGTATCGACGGACAGGACATCAGGACCATCAATGTGAGGTATCTGCGGGAAAT CATTGGGGTGGTGAGTCAGGAACCCGTGCTGTTTGCCACCACGATTGCCGAAAACATTCGCTATGGCCGA GAAAACGTCACCATGGATGAGATAGAGAAAGCTGTCAAGGAAGCCAATGCCTATGACTTCATCATGAAAC TGCCCCACAAATTTAACACCCTGGTTGGTGAGAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAGAGGAT CGCCATTGCCCGGGCCCTGGTCCGCAACCCCAAGATCCTTTTGTTGGATGAGGCCACGTCAGCCTTGGAC ACAGAAAGCGAAGCCGTGGTTCAGGCCGCTCTGGATAAGGCTAGAGAAGGCCGGACCACCATTGTGATAG CTCACCGCTTGTCTACAGTGCGCAATGCTGACGTCATTGCTGGTTTTGATGGTGGTGTCATTGTGGAGCA AGGAAATCATGAAGAGCTCATGAAAGAGAAGGGCATTTACTTCAAACTTGTCATGACACAGACTAGAGGA AATGAAATTGAACCAGGAAATAATGCTTATGAATCCCAAAGTGACACTGGTGCCTCTGAGTTGACTTCAG AAAAATCAAAATCTCCTTTAATAAGGAGATCAATTCGCAGAAGTATCCACAGAAGACAAGACCAGGAGAG AAGACTTAGTTCGAAAGAGGATGTGGATGAAGATGTGCCTATGGTTTCCTTTTGGCAGATCCTAAAGCTA AATATTAGTGAATGGCCCTATTTAGTTGTGGGTGTACTTTGTGCTGTTATAAATGGGTGCATACAACCAG TGTTTGCCATAGTGTTTTCAAAGATTGTAGGGGTTTTTTCAAGAGACGACGACCATGAAACCAAACAACG GAATTGTAACTTGTTTTCCCTTCTCTTTCTGGTCATGGGAATGATTTCTTTTGTTACGTACTTCTTTCAA GGCTTCGCATTTGGCAAAGCTGGAGAGATCCTCACCAAGCGACTCCGATACATGGTCTTCAAATCCATGC TGCGACAGGATATAAGCTGGTTTGATGACCATAAAAACACCACTGGCTCGCTGACTACCAGGCTCGCTAG TGACGCTTCTAATGTTAAAGGGGCTATGGGCTCCAGGCTTGCTGTAGTTACCCAGAATGTAGCAAACCTT GGCACAGGAATTATCTTATCCTTAGTCTATGGCTGGCAGCTTACACTTTTACTTGTAGTAATTATACCAC TCATTGTCTTGGGTGGAATTATTGAAATGAAACTGTTGTCTGGTCAAGCCTTGAAGGACAAGAAAGAGCT AGAGATCTCTGGGAAGATCGCTACAGAAGCAATTGAAAACTTCCGCACTGTTGTCTCTTTGACTCGGGAG CAGAAGTTTGAAACTATGTATGCCCAGAGCTTGCAGATACCATACAGAAATGCTTTGAAGAAAGCACACG TCTTTGGGATCACCTTCGCCTTCACCCAGGCCATGATTTATTTCTCCTATGCTGCTTGTTTCCGGTTCGG TGCCTACTTGGTGGCACGAGAACTCATGACATTTGAAAATGTTATGTTGGTATTTTCTGCTGTTGTCTTT GGTGCCATGGCAGCAGGGAATACCAGTTCATTCGCTCCTGACTACGCGAAGGCCAAAGTCTCAGCATCCC ACATCATCAGGATCATTGAGAAAATCCCCGAGATTGACAGCTACAGCACGGAGGGCTTGAAGCCTAATTG GTTAGAAGGAAATGTGAAATTTAATGGAGTCATGTTCAACTATCCCACCCGACCCAACATCCCAGTGCTT CAGGGACTGAGCTTCGAGGTGAAGAAGGGGCAGACGCTCGCCCTGGTGGGCAGCAGTGGCTGCGGGAAGA GCACGGTGGTCCAGCTGCTCGAGCGCTTCTACAACCCCATGGCTGGAACAGTGTTTCTAGATGGCAAAGA AATAAAACAACTCAACGTCCAGTGGCTCCGCGCCCACCTGGGCATTGTGTCCCAGGAGCCCATCCTGTTT GACTGCAGCATCACCGAGAACATCGCCTACGGAGACAACAGCCGTGTCGTGTCTCATGAGGAGATCGTGA GGGCCGCCAGGGAGGCCAACATCCACCAGTTCATCGACTCACTGCCTGAGAAATACAACACCAGAGTGGG AGACAAAGGGACTCAGCTGTCGGGCGGGCAGAAGCAGCGCATCGCCATCGCGCGCGCCCTCGTCAGACAG CCTCACATCTTACTTCTGGATGAAGCGACATCAGCTCTGGATACGGAGAGTGAAAAGGTCGTCCAGGAAG CGCTGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCGCACCGCCTGTCCACCATCCAGAACGC AGACTTGATCGTGGTGATTCAGAACGGCCAGGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCCCAG AAAGGCATCTATTTCTCGATGGTTCAGGCTGGAGCAAAGCGCTCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012623
Insert Size	3828 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012623.2, NP_036755.2
RefSeq Size	3888 bp
RefSeq ORF	3828 bp
Locus ID	24646
Gene Summary	member of the ATP-binding cassette (ABC) protein superfamily; may play a role in drug disposition [RGD, Feb 2006]

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