Sgms2 (NM_001014043) Rat Untagged Clone

CAT#: RN211313

Sgms2 (untagged ORF) - Rat sphingomyelin synthase 2 (Sgms2), (10 ug)



  "NM_001014043" in other vectors (3)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms RGD1305778; spermatin
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211313 representing NM_001014043
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATATCATAGAGACAGCAAAACTTGAAGGTCATTTGGAAAGTCAAACCAACAACTCTACCAACACTT
ACACAAGCCCCACTGAAGCTGTAGAAGAGGAGGACAAAAATGGCAAGGGTAAACCCAAGACCTTATCCAA
CGGGCTACGAAAGGGTGCCAAGAAATACCCGGACTACATCCAGATTTCCATGCCCAATGACTCCAGGAAC
AAGCTTCCCCTAGAGTGGTGGAAAACAGGCATTGCCTTTGTGTACGCTCTCTTCAACCTTATCCTGACAA
CAGTCATGATCACCGTTGTGCACGAGAGGGTCCCTCCCAAGGAACTCAGCCCTCCACTCCCAGACAAGTT
TTTTGATTACGTCGACCGGGTCAAATGGGCATTTTCTGTATCAGAAATAAATGGGATGGTATTAGTTGGG
CTATGGCTCACCCAGTGGCTCTTTCTGAGATACAAGTCAATAGTGGGACGCAGATTCTTCTTTATCATGG
GAACTTTATACCTGTATCGCTGTATAACTATGTACGTCACTACGTTACCTGTGCCCGGAATGCACTTCCA
GTGTGCTCCAAAGCTCAATGGAGACTCTCAGGCAAAAATACAACGGATTCTCCGGTTGCTTTCTGGTGGT
GGATTGTCCATCACGGGATCACACATCCTGTGTGGAGACTTCCTCTTCAGCGGCCATACAGTTGTGCTGA
CACTTACTTACTTGTTCATCAAAGAATATTCACCTCGTCACTTCTGGTGGTACCACTTGGTCTGCTGGCT
GCTGAGCGCCGCTGGGATCGTCTGCATCCTCGTGGCGCACGAACACTACACGGTCGATGTCATCATTGCG
TATTACATCACAACACGGCTGTTTTGGTGGTACCACTCCATGGCCAATGAAAAGAACTTGAAGGTCTCTT
CCCAGACTAATTTCTTGTCTCGGGCTTGGTGGTTCCCCATCTTCTATTTTTTTGAAAAGAATGTGCAAGG
CTCCATTCCTTGCTGTTTCTCCTGGCCGCTGTCCTGGCCCCCTGGCTGCTTCAAGTCATCGTGCAAAAAG
TATTCTCGAGTCCAGAAGATCGGGGAGGATAATGAGAAGTCTACCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001014043
Insert Size 1098 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001014043.1, NP_001014065.1
RefSeq Size 1924 bp
RefSeq ORF 1098 bp
Locus ID 310849
UniProt ID Q4JM44
Gene Summary Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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