Cacna1g (NM_031601) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211239 representing NM_031601 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGGAGGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGTAGCTTCACGCAGCTCAACG ACCTGTCCGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA GGCGGAGGGGCTGCCGTACCCGGCGCTAGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGTATGCTGGTCATTCTTCTCA ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAGCGCTGCCGGATCCT GCAGGCCTTCGATGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTGGCCTTGGGC ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTCATTGTCATTGCAGGGA TGCTGGAGTATTCGCTGGACCTGCAGAACGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGACACCTTGCCTATG CTGGGCAACGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTGGGCGTCCAGCTGTGGG CAGGACTGCTTCGCAACCGATGCTTCCTCCCCGAGAACTTCAGCCTCCCCCTGAGCGTGGACCTGGAGCC TTATTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAATGGCATGAGA TCCTGCAGGAGTGTGCCCACACTGCGTGGGGAAGGCGGTGGTGGCCCACCCTGCAGTCTGGACTATGAGA CCTATAACAGTTCCAGCAACACCACCTGTGTCAACTGGAACCAGTACTATACCAACTGCTCTGCGGGCGA GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC ATCACACTGGAGGGCTGGGTCGACATCATGTACTTCGTAATGGACGCTCACTCCTTCTACAACTTCATCT ACTTCATTCTTCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATTGCCACGCA GTTCTCCGAGACCAAACAGCGGGAGAGTCAGCTGATGCGGGAGCAGCGTGTACGATTCCTGTCCAATGCT AGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTACTCAAGTACCTGGTGTACATCC TCCGAAAAGCAGCCCGAAGGCTGGCCCAGGTCTCTAGGGCTATAGGCGTGCGGGCTGGGCTGCTCAGCAG CCCAGTGGCCCGTAGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCACTCGCTCACACCGTCGTCTGTCT GTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTGGGTAATGGGACGCTCAGAG TTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCTCGCCGGCTCATGCTACCACCACC CTCTACACCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACACAGCTTCTACCATGCTGAC TGCCACTTGGAGCCAGTCCGTTGCCAGGCACCCCCTCCCAGATGCCCATCGGAGGCATCTGGTAGGACTG TGGGTAGTGGGAAGGTGTACCCCACTGTGCATACCAGCCCTCCACCAGAGATACTGAAGGATAAAGCACT AGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACATCCCACCTGGGCCCTTCAGC TCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCCTGCAAAATCTCCAGCCCTT GCTCCAAGGCAGACAGTGGAGCCTGCGGGCCGGACAGTTGTCCCTACTGTGCCCGGACAGGAGCAGGAGA GCCAGAGTCCGCTGACCATGTCATGCCTGACTCAGACAGCGAGGCTGTGTATGAGTTCACACAGGACGCT CAGCACAGTGACCTCCGGGATCCCCACAGCCGGCGGCGACAGCGGAGCCTGGGCCCAGATGCAGAGCCTA GTTCTGTGCTGGCTTTCTGGAGGCTGATCTGTGACACATTCCGGAAGATCGTAGATAGCAAATACTTTGG CCGGGGAATCATGATCGCCATCCTGGTCAATACACTCAGCATGGGCATCGAGTACCACGAGCAGCCCGAG GAGCTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCCTTGGAGATGCTGCTGA AACTGCTTGTCTACGGTCCCTTTGGCTACATTAAGAATCCCTACAACATCTTTGATGGTGTCATTGTGGT CATCAGTGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCGGACCTTCCGCCTGATG CGGGTGCTGAAGCTGGTGCGCTTCCTGCCGGCCCTGCAGCGCCAGCTCGTGGTGCTCATGAAGACCATGG ACAACGTGGCCACCTTCTGCATGCTCCTCATGCTGTTCATCTTCATCTTCAGCATCCTGGGCATGCATCT CTTTGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAAGAATTTCGACTCCCTG CTCTGGGCCATCGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAAGTCCTCTACAACGGCA TGGCCTCCACATCGTCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTGGCAACTATGTGCTCTT TAACCTGCTGGTGGCCATTCTTGTGGAAGGCTTCCAGGCAGAGGGAGATGCCACCAAGTCTGAGTCAGAG CCTGATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGAAAGAAGCGCTTGGCCCTGGTGGCTTTGG GAGAACACGCGGAACTACGAAAGAGCCTTTTGCCACCCCTCATCATCCATACGGCTGCGACACCAATGTC ACTACCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCGACGTACCAGTAGCAGT GGGTCCGCTGAGCCTGGAGCTGCCCACCATGAGATGAAATCTCCGCCAAGTGCCCGCAGCTCCCCGCACA GTCCCTGGAGTGCGGCAAGCAGCTGGACCAGCAGGCGCTCCAGCAGGAACAGCCTGGGCCGGGCCCCCAG CCTAAAGCGGAGGAGCCCGAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGGCCAGGAGAGTCAGGAT GAGGAGGAAAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGACCATCGCCACAGGGGTTCCTTGG AACGTGAGGCCAAGAGTTCCTTTGACCTGCCTGACACTCTGCAGGTGCCGGGGCTGCACCGCACAGCCAG CGGCCGGAGCTCTGCCTCTGAGCACCAAGACTGTAATGGCAAGTCGGCTTCAGGGCGTTTGGCCCGCACC CTGAGGACTGATGACCCCCAACTGGATGGGGATGATGACAATGATGAGGGAAATCTGAGCAAAGGGGAAC GCATACAAGCCTGGGTCAGATCCCGGCTTCCTGCCTGTTGCCGAGAGCGAGATTCCTGGTCGGCCTATAT CTTTCCTCCTCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAAGATGTTTGACCATGTG GTCCTCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAAATTGACCCCCACAGCG CTGAGCGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTTCTAGCTGAAATGACAGTGAA GGTGGTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTGGAATGTGCTGGACGGC TTGCTGGTGCTCATCTCCGTCATCGACATCCTGGTCTCCATGGTCTCCGACAGCGGCACCAAGATCCTTG GCATGCTGAGGGTGCTGCGGCTGCTGCGGACCCTGCGTCCACTCAGGGTCATCAGCCGGGCCCAGGGACT GAAGCTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGTGGTCATTTGCTGTGCC TTCTTCATCATTTTTGGAATTCTCGGGGTGCAGCTCTTCAAAGGGAAGTTCTTCGTGTGTCAGGGTGAGG ACACCAGGAACATCACTAACAAATCCGACTGCGCTGAGGCCAGCTACCGATGGGTCCGGCACAAGTACAA CTTTGACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGATGGTTGGGTTGACATC ATGTATGATGGGCTGGATGCTGTGGGTGTGGATCAGCAGCCCATCATGAACCACAACCCCTGGATGCTGC TATACTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTTGTCCTGAACATGTTTGTGGGCGTGGTGGTGGA GAACTTCCATAAGTGCAGACAGCACCAGGAGGAGGAGGAGGCGAGGCGGCGTGAGGAGAAGCGACTACGG AGGCTGGAGAAAAAGAGAAGGAGTAAGGAGAAGCAGATGGCCGAAGCCCAGTGCAAGCCCTACTACTCTG ACTACTCGAGATTCCGGCTCCTTGTCCACCACCTGTGTACCAGCCACTACCTGGACCTCTTCATCACTGG TGTCATCGGGCTGAACGTGGTCACTATGGCCATGGAACATTACCAGCAGCCCCAGATCCTGGACGAGGCT CTGAAGATCTGCAATTACATCTTTACCGTCATCTTTTGTCCTTTGAGTCAGTTTTCAAACTTGTGGGCCT TTGGCTTCCGCCGTTTCTTCCAGGACAGGTGGAACCAGCTGGACCTGGCTATTGTGCTTCTGTCCATCAT GGGCATCACACTGGAGGAGATTGAGGTCAATGCTTCGCTGCCCATCAACCCCACCATCATCCGTATCATG AGGGTGCTCCGCATTGCTCGAGTTCTGAAGCTGTTGAAGATGGCTGTGGGCATGCGGGCACTGCTGGACA CGGTGATGCAGGCCCTGCCCCAGGTGGGGAACCTGGGACTTCTCTTCATGTTATTGTTTTTCATCTTTGC AGCTCTGGGCGTGGAGCTCTTTGGAGACCTGGAGTGTGATGAGACACACCCTTGTGAGGGCTTGGGTCGG CATGCCACCTTTAGGAACTTTGGTATGGCCTTTCTGACCCTCTTCCGAGTCTCCACTGGTGACAACTGGA ATGGTATTATGAAGGACACCCTCCGGGACTGTGACCAGGAGTCCACCTGCTACAACACTGTCATCTCCCC TATCTACTTTGTGTCCTTCGTGCTGACGGCCCAGTTTGTGCTGGTCAACGTGGTCATAGCTGTGCTGATG AAGCACCTGGAAGAAAGCAACAAAGAGGCCAAGGAGGAGGCCGAGCTCGAGGCCGAGCTGGAGCTGGAGA TGAAGACGCTCAGCCCGCAGCCCCACTCCCCGCTGGGCAGCCCCTTCCTCTGGCCCGGGGTGGAGGGTGT CAACAGTCCTGACAGCCCTAAGCCTGGGGCTCCACACACCACTGCCCACATTGGAGCAGCCTCGGGCTTC TCCCTTGAGCACCCCACGATGGTACCCCACCCCGAGGAGGTGCCAGTCCCCCTAGGACCAGACCTGCTGA CTGTGAGGAAGTCTGGTGTCAGCCGGACGCACTCTCTGCCCAATGACAGCTACATGTGCCGCAATGGGAG CACTGCTGAGAGATCCCTAGGACACAGGGGCTGGGGGCTCCCCAAAGCCCAGTCAGGCTCCATCTTGTCC GTTCACTCCCAACCAGCAGACACCAGCTGCATCCTACAGCTTCCCAAAGATGTGCACTATCTGCTCCAGC CTCATGGGGCCCCCACCTGGGGCGCCATCCCTAAACTACCCCCACCTGGCCGCTCCCCTCTGGCTCAGAG GCCTCTCAGGCGCCAGGCAGCAATAAGGACTGACTCCCTGGATGTGCAGGGCCTGGGTAGCCGGGAAGAC CTGTTGTCAGAGGTGAGTGGGCCCTCCTGCCCTCTGACCCGGTCCTCATCCTTCTGGGGCGGGTCGAGCA TCCAGGTGCAGCAGCGTTCCGGCATCCAGAGCAAAGTCTCCAAGCACATCCGCCTGCCAGCCCCTTGCCC AGGCCTGGAACCCAGCTGGGCCAAGGACCCTCCAGAGACCAGAAGCAGCTTAGAGCTGGACACGGAGCTG AGCTGGATTTCAGGAGACCTCCTTCCCAGCAGCCAGGAAGAACCCCTGTCCCCACGGGACCTGAAGAAGT GCTACAGTGTAGAGACCCAGAGCTGCAGGCGCAGGCCTGGGTCCTGGCTAGATGAACAGCGGAGACACTC CATTGCTGTCAGCTGTCTGGACAGCGGCTCCCAACCCCGCCTATGTCCAAGCCCCTCAAGCCTCGGGGGC CAACCTCTTGGGGGTCCTGGGAGCCGGCCTAAGAAAAAACTCAGCCCACCCAGTATCTCTATAGACCCCC CGGAGAGCCAGGGCTCTCGGCCCCCATGCAGTCCTGGTGTCTGCCTCAGGAGGAGGGCGCCGGCCAGTGA CTCTAAGGATCCCTCGGTCTCCAGCCCCCTTGACAGCACGGCTGCCTCACCCTCCCCAAAGAAAGACACG CTGAGTCTCTCTGGTTTGTCTTCTGACCCAACAGACATGGACCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031601
Insert Size	6768 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031601.4, NP_113789.3
RefSeq Size	7657 bp
RefSeq ORF	6768 bp
Locus ID	29717
Gene Summary	low voltage-activated calcium channel alpha subunit; involved in calcium ion transport in nerve, muscle and edocrine cells [RGD, Feb 2006] Transcript Variant: This variant (2) lacks two in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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