Washc2c (NM_199207) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Fam21; Fam21c; NP61201; Washc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210867 representing NM_199207 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGGACGAGCCCGGACTCGGAGCAGCCTCCGGCGTCCGAACCCGTGTGGGAACGGCCCTGGTCGG TGGAGGAGATCCGCCGCAGCAGTCAGAACTGGTCGCTGGCGGCTGACGCTGGTCTTCTACAGTTTCTACA GGAGTTCTCACAGCAGACTATCTCCAGGACTCACGAAATCAAGAAGCAGGTGGATGGATTAATTCAGGAG ACCAAGGCCACACATTGTCGTCTGCATAATGTCTTCAACGACTTCCTCATGCTGTCTAACACCCAGTTCA TTGAGAATCGGGTGTACGATGAGGAAGTGGAGGATCAGGCGCTCAAGACGGAGGCGGAAAAAGCTGAGCA GGAGAAGACTCGGGAACAGAAAGAAATAGATCTGATTCCTAAAGTCCAGGAGGCTGTGAACTATGGGTTA CAGGTCCTGGATAGTGCGTTTGAGCAACTTGACATAAAAGCAGGGAATTCGGACTCTGAAGAGGAAGATG CCAATGAGCGGGTGGAATTGATCCTTGAGCCAAAGGACCTCTACATTGACCGGCCTTTGCCCTACCTCAT CGGGTCGAAGCTCTTCATGGAACAGGAAGACGTGGGGCTTGGAGAGCTGTCCAGCGAAGAAGGCTCTGTG GGCAGTGACCGCGGGAGCATTGTGGACAGTGAAGATGAGAAAGAAGAGGAGGAGTCAGATGATTTTGCCA GTCATAGTGACAATGAACAAAACCAGCATATCACACAGATGAGTGACGAAGAAGAGGATGATGATGCTGA CCTCTTTGCTGACTCTGAGAAGGAGGGGGATGATATCGAGGATATTGAGGAAAGTGCTAAGTCTAAAAGA CCTACGTCTTTTGCGGATGAGCTGGCTGCTCGGATCAAAGGAGATGTCTCAAACCAGCGGAAAGAGGGAC ACACAGATGGAAAGCCTCAGAGGACAGTGAAGGAGAAGAAGGAAAGGAGAACCCCTGCAGATGATGAGGA AGACATCTTGTTCCCACCCCCCACGCTGACTGATGAAGACTTCTCACCGTTTGGCTCTAGAGGTGGTCTG TTCAGTGATCGGCAGGGGCTTTTTGATGATGATGATGAGAGTGACCTCTTCAAGGAAGCTCCCCGGGGGC AGCCAGCCCAAGGTCCTGTCAGTGAAGAATCACCTCCATCCCCTAAACCTGGAAAGAAAATCCCAGCAGG GGCTGTTTCTGTATTTTTAGGGTATACTGATGTGTCTGGGTCCACCTCTGCCCCATCACTGAAGGAGTTC CAGAAACATGAGCAGTCCACTCCAGGGAAGAGCCCACATTTGCCAGCTCCTACTGGCCTCTTTGATGATG ATGATAACGACAGTGATGAAGATGATAACTTTTTTATGCCATCTTCTAGCAAGCCTTCTAAGACCGACAA AGTCAAGCCCACCACCATTATCTTTGATGATGACGAAGGAGATCTGTTTAAAGAAAAAACAACAGCTTTG CCAGCAGCTTCTGTCAGCCAGACAGATGAAAACAAAGCACGGACAGATAAAACGATCACCTTACCTTCTA GCAAAAATCCGAAGCTTGTATCAGAAACAAAGACTCAGAAAGGTTTGTTTTCAGATGAAGAAGACTCTGA GGATTTGTTTTCTTCTCAAAGTTCAAGTAAGACGAAAAGTGCATCAGTTCTGTCCAGCCAACCCCCAGCA TCGGTCTCCCTTTTTGGTGATGAAGATGAAGAGGACAATCTTTTTGGGAGTGCAGCAGCTAAGAAGCAGA CTTCATCTCTGCCACCTCAGAGTCAGGAGAAAGCAAAACCTTCAGAGCAGCCCCCAAAGAAAGCATCTGC CTTGTTCAGCAGTGACGAGGAGGACCAGTGGAGTGTTGCTGATTCACAGACCAAGTTAGCCTCCGAGAGA AAGTCCAAAGGAGAGAGGTGGGACGCGGGGACCAACCAAGGCCAAGAAGCGAAGGCTGTGAAAAAGACCA ATCTCTTTGAGGAGGAGGATGATGATGGAGTCGATCTCTTTGCTATTGCGAAGGACAGCCAAAAGAAGAC TCAGAGAACCTCACTTCTGTTTGAAGATGACACTGATAGCGGAAGCTCTCTGTTCAGCCTTCCTCCTACA TCTGTTCCTCCTGCAGCAACGAAAAAAGAGAGCATCCCCAAGGTGCCGTTGTTGTTTAGTGATGAAGAGG ACAGTGAGGTACCATCTGGTGTGAAACCTGTGGATCTGAAGGCTGAGAATGCCGCGGCGTCCCCAGAGGT GGGGAGTGCTGATGTGGCCAATGTGGCACAGAAGGAAGGACTTTTACCCACATCTGATCAGGAAGCAGGC GGGCCTTCTGATATATTTTCTTCTTCATCTCCACTGGACAAAGGAGCCAAGGGTAGGACCAAAACTGTCC TTAGTTTGTTTGATGAGGACGAGGACAAAGTAGAAGATGACAGCAACACCTGTGCGCCACAAGGTGGACT GGAAAAGGGTGTCAAAACTGACCGCCGCCCTAAGAGCACAGGAGTCTTTCAGGATGAAGAGCTGCTTTTC AGCCACAAGCTGCAAAAGGACAACGACCCAGATGTTGATCTTTTCGCTGGTACCAAAAAAACCAGGTTGT CCATGCCAAGTGGGGGGAGCCTCTTTGGTGATGACGACGATGATGATCTCTTCAGCACTGCCAAGACTCA GCCTGCACAGCCTGTGGTACCAGAAAAAAAAGGAACACTGAGAAAAGACCACAAACCTCCTGAATTGACT GAAGGTAGCAAAGAGAAAAGCACATGGAAAGCAGAAACGGCCCAGGATTCTTCAGGTCTCACTCCATTTA AAAGCAGAGAGCCATCCAGTCGCATCGGGAAAATACAAGCAAATTTAGCAATTAACCCAGCCACTTTGCT GCCCTCAGTGGCTCCGCAGATCCCCGGAGCAAAGCCTGCTTCGTGTGAACTAGCTTTTCCTTCATCTGAG CCTGCAAGGAGCCACATCCGGGAAGCTGTGCCCACTCTTCCTGGGAGTGAGGAGGCTGGTGTGAGTTTCG ATCTTCCTGCCCAGGCAGACACCTTACACAGTGCAAACAAGGGTCGTGTCAAAGTGAGAGGAAAGAGGAG ACCACAGACCCGTGCTGCTCGGCGGCTGGCTGCCCAGGAGTCCAGCGAGGCTGAGGACGTGACCATTGAT AGAGGACCTGTTACACAGTTGAGCAGTAGTCCTGTCTTACCAAACGGCCATCAACCTCTTCTGCAGCCTA GGATGGCCAGTGGAGAGACCAGCTCTGAAAAAGCCATGGCCGTACCTTGGGAAGGTGGTCCTGTTTTATC TGCAGTGGACAGAAGCTTCTTTGTAAAGTCTCTGCCTCAGACTGGAAATGAGGCCCACCTGTTTGATTCT GGGGACATTTTCCCCAAGAGCACAGGATCACAGTCCATGGAGGGAGCAAGTGTCAAGGCAGGGGAGACCC CAGCCCACTCGTCAGCAGGGCGGAAGGAGAAGAGCCTGGTATTTCCTGATCTTAGTGAGGCCAGCGGTGT CGATGACCTTTTCCAGTCTGCCAAGCCAAGACCAACAAAGAAAAGAAACCCTTTCCCCCTCCTTGAAGAT GAGGAGGACCTCTTTGCAGATCAGAAAGGCAAAAAGAATCAGTGGAAATCTGACAGCCATCAGGACGTTG TGTCAAAAACTCAAGACATCTTTGAGGATGATATATTTGCTACGGAAGCAATTAAGAAGCCTTTTCCAAA AAAAAGAGAGAAGGAGAGAACTTTGGAACCCAACTTATTTGATGATAATATTGATATCTTCGCTGACTTG ACTGTGAAACCCAAAGAAAAGCCCAAAAAGAAAGTCACAGCCAAGTCCATGTTTGACGATGATACAGATG ATATTTTCTCCTCTGGCCTGCAGGCTAAAGCATCTAAACCCAAAAGCCAATCTGCTGAGGCAGTATCTGA ATTAAGGTCTGAGAACAAAGTGTCCAACATTTTTGATGACCCTCTGAATGCCTTTGGAAGCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_199207
Insert Size	3987 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_199207.1, NP_954677.1
RefSeq Size	4269 bp
RefSeq ORF	3987 bp
Locus ID	297530
UniProt ID	Q80X08
Gene Summary	Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes inhibits WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization and is involved in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission. Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1. Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35. Required for the endosomal recruitment of CCC and retriever complexes subunits COMMD1 and CCDC93 as well as the retrievere complex subunit VPS35L.[UniProtKB/Swiss-Prot Function]

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