Rag1 (NM_053468) Rat Untagged Clone

CAT#: RN210714

Rag1 (untagged ORF) - Rat recombination activating gene 1 (Rag1), (10 ug)



  "NM_053468" in other vectors (3)

CNY 16,720.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN210714 representing NM_053468
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTCCCCTTGCCATCTACCCTGAGACTCAGTTCTGCACCTGATGAAATTCAGCACCCGCACATCA
AATTTTCCGAGTGGAAATTTAAGCTGTTTAGGGTGAGATCCTTTGAAAAGGCACCCGAAGAAGCACAAAA
AGAGAAGGATTCCTCAGAAGGGAAACCTTGTCTCGAGCAGTCTCCAGTAGTTCTAGATAAGCCTGGGGGT
CAGAATTCAGTTCTGACTCAACGAGCATTGAAACTCCATCCTAAATTTTCAAAGAAATTCCATGTTGATG
GGAAGTCAAGCGACAAAGCAATTCACCAAGCCAGGCTTAGACACTTCTGCCGCATCTGTGGCAATCACTT
CAAGAGTGACGGGCACAACCGGAGATACCCAGTCCACGGGCCCGTGGACGCTAAAACTCAAAGCCTTTTC
CGAAAGAAGGAAAAAAGAGTCACGTCCTGGCCAGATCTCATTGCCAGAGTTTTCCGGATTGATGTGAAGT
CAGATGTTGACTCCATCCACCCCACTGAATTCTGCCATAACTGTTGGAGCATTATGCACAGGAAGTTCGG
CAGTGCTCACAGTCAGGTCTACTGCCCAAGGAATGTGACCGTGGAGTGGCACCCCCACACACCGTCCTGT
GACATCTGCTTTACTGCCCATCGGGGACTGAAGAGGAAGAGACATCAGCCCAACGTGCAGCTCAGCAAGA
AACTAAAAACTGTGCTCAACCATGCTAGACGGGACCGTCGCAAGAGAACTCAGGCTAGGGTCAGCAGCAA
GGAGGTCATGAAGAAGATCTCCAACTGCAGTAAGATTCATCTCAGTACCAAGCTTCTTGCAGTAGACTTC
CCAGCACACTTTGTGAAATCCATCTCCTGCCAGATATGTGAACACATTCTGGCCGATCCGGTGGAGACCA
CCTGTAAGCACCTGTTCTGTAGAATATGCATTCTCCGGTGCCTCAAGGTCATGGGCAGCTATTGTCCCTC
GTGCCGATATCCATGCTTCCCTACTGACCTGCAGAGTCCGGTCAAGTCCTTTCTGAGCATCTTGAATTCT
CTGATGGTGAAGTGTCCTGCCCAAGAGTGCAATGAGGAAGTAAGTCTGGAAAAATATAACCACCATGTCT
CAAGCCACAAAGAATCTAAAGAGACTTTGGTGCATATTAATAAAGGGGGCCGGCCTCGCCAGCATCTCCT
GTCACTGACGCGAAGGGCTCAGAAACATCGGCTGAGGGAGCTCAAGATTCAAGTCAAAGAATTTGCAGAC
AAAGAAGAAGGTGGAGATGTGAAGTCTGTGTGCTTGACGTTGTTCCTGCTGGCACTGAGGGCAAGGAATG
AGCACAGACAAGCTGATGAGCTCGAGGCCATCATGCAAGGCCGGGGCTCCGGTCTTCAACCAGCTGTTTG
CTTGGCCATCCGTGTCAATACCTTCCTCAGCTGTAGCCAATACCATAAGATGTACAGGACGGTGAAAGCT
ATCACTGGGAGGCAGATTTTTCAACCTTTGCACGCTCTTCGCAATGCTGAGAAGGTCCTCCTACCAGGCT
ACCACCCCTTTGAGTGGCAGCCCCCTCTGAAGAACGTGTCCTCCAGAACTGACGTTGGAATTATTGATGG
GCTCTCTGGACTTGCCTCCTCCGTAGATGAATACCCAGTGGACACAATTGCAAAGAGGTTCCGCTATGAC
TCTGCTTTGGTGTCTGCTTTGATGGACATGGAGGAAGACATCTTGGACGGCATGAGATCCCAAGATCTTG
ATGACTACGTGAGTGGTCCCTTCACCGTGGTGGTAAAGGAATCGTGTGATGGAATGGGGGATGTGAGTGA
GAAGCACGGGAGTGGGCCCGCAGTTCCAGAAAAGGCAGTTCGGTTCTCTTTCACCGTCATGAGAGTTACA
GTAGAGCATGGCTCTCAGAACGTGAAGATATTTGAGGAACCCAAGCCCAATTCTGAACTGTGTTGCAAGC
CACTGTGCCTTATGCTGGCAGATGAGTCTGACCACGAGACACTTACTGCTATTCTCAGCCCCCTCATCGC
TGAGAGGGAGGCCATGAAGAGCAGTGAATTGCTGCTGGAGATGGGAGGCATCCCCAGGACTTTTAAATTT
ATCTTCAGGGGCACTGGATATGACGAGAAACTTGTCAGGGAAGTAGAAGGCTTGGAAGCTTCTGGCTCAG
TCTACATTTGTACACTTTGTGATGCCACCCGTCTGGAAGCCTCCCAAAATCTTGTCTTCCACTCCATAAC
CAGAAGCCACGCCGAGAATCTGCAGCGCTATGAGGTCTGGCGGTCCAATCCCTATCACGAGTCAGTGGAA
GAACTCCGGGACCGGGTGAAAGGAGTCTCTGCCAAACCTTTCATCGAGACAGTCCCTTCCATAGATGCGC
TTCACTGTGACATTGGAAATGCAGCTGAATTCTATAAGATTTTCCAGCTGGAGATAGGGGAAGTGTATAA
AAATCCCAATGCCTCTAAAGAGGAAAGGAAGAGATGGCAGGCCACCTTGGACAAACATCTGCGGAAAAGG
ATGAACTTAAAACCAATCATGAGGATGAATGGCAACTTTGCCCGGAAGCTTATGACCCAAGAGACTGTGG
ACGCAGTTTGTGAGTTAATTCCTTCTGAGGAGAGGCACGAAGCTCTCAGGGAGCTGATGGACCTTTACCT
GAAGATGAAACCGGTGTGGCGCTCATCGTGTCCTGCTAAAGAGTGCCCGGAGTCCCTTTGTCAGTACAGT
TTCAACTCACAGCGTTTCGCTGAGCTCCTCTCCACCAAGTTCAAATACAGATACGAGGGCAAAATCACCA
ATTACTTTCACAAAACCTTGGCACACGTCCCTGAAATTATTGAAAGGGATGGCTCTATTGGGGCATGGGC
AAGTGAGGGCAATGAGTCTGGTAACAAACTGTTTAGGCGCTTCCGGAAAATGAATGCCAGGCAGTCCAAG
TGCTATGAGATGGAAGATGTCCTGAAACACCACTGGCTGTATACTTCAAAATACCTCCAGAAGTTTATGA
ATGCCCATAATGCATTAAAAAACTCTGGGTTTACCATGAACTCAAAGGAGACTTTAGGGGACTCTTTGGA
CATTGAGGACTCTCTGGAAAGCCAAGATTCAATGGAGTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053468
Insert Size 3123 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053468.1, NP_445920.1
RefSeq Size 6537 bp
RefSeq ORF 3123 bp
Locus ID 84600
Gene Summary expressed in intestinal intraepithelial T lymphocytes [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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