Erbb3 (NM_017218) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	nuc-ErbB3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210665 representing NM_017218 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCGACTGGGACTCTGCAGGTGCTGTGCTTCCTTCTCAGCCTGGCCCGGGGTTCCGAGATGGGCA ACTCTCAGGCAGTGTGTCCTGGGACTCTGAACGGACTGAGTGTGACCGGAGATGCAGACAACCAGTACCA GACGCTGTACAAACTGTATGAAAAGTGTGAGGTGGTCATGGGTAACCTGGAGATTGTGCTCACAGGACAC AATGCTGACCTTTCCTTCCTGCAATGGATCCGAGAAGTGACAGGCTACGTCCTCGTGGCCATGAATGAAT TCTCTGTGCTTCCCTTGCCTAACCTCCGAGTGGTCCGGGGAACCCAGGTCTACGATGGGAAGTTCGCCAT CTTTGTTATGTTGAACTACAACACCAACTCCAGCCATGCTCTGCGCCAGCTCAAGTTCACTCAGCTAACA GAGATTCTGTCAGGGGGCGTTTACATTGAAAAGAATGACAAACTTTGCCACATGGATACAATTGACTGGA GAGACATCGTGAGGGTTCGAGGCGCTGAGATAGTGGTGAAGAACAATGGTGCGAACTGTCCACCCTGTCA TGAGGTCTGCAAGGGACGGTGCTGGGGTCCTGGACCGGACGACTGCCAGATATTGACCAAGACGATCTGT GCCCCTCAGTGTAACGGTCGCTGCTTCGGGCCCAACCCTAACCAGTGCTGTCACGATGAGTGTGCTGGGG GCTGCTCTGGACCTCAGGACACAGATTGCTTTGCCTGCCGACGCTTCAATGACAGTGGGGCCTGTGTGCC CCGGTGTCCAGAGCCTCTTGTGTACAACAAGCTAACGTTTCAGCTTGAACCCAACCCCCATACCAAGTAT CAGTATGGAGGAGTCTGCGTTGCCAGTTGTCCCCATAACTTTGTGGTGGACCAGACGTTTTGTGTCAGGG CTTGTCCTCCTGACAAGATGGAAGTAGATAAGCACGGACTGAAGATGTGTGAGCCTTGCGGAGGGCTGTG CCCCAAAGCCTGTGAGGGGACAGGCTCTGGAAGCCGCTACCAGACTGTGGACTCAAGCAACATTGATGGG TTCGTGAACTGTACCAAGATCCTGGGCAATCTGGACTTCCTCATCACCGGCCTCAACGTTGACCCCTGGC ATAAGATACCCGCACTGGACCCGGAAAAGCTCAATGTTTTCAGAACAGTCCGGGAGATCACAGGTTACCT AAACATCCAGTCCTGGCCCCCTCACATGCACAACTTCAGTGTTTTTTCCAATCTGACAACCATCGGGGGC AGAAGCCTCTACAACCGGGGCTTCTCCTTGTTGATCATGAAGAACTTGAATGTCACATCCCTGGGTTTCC GGTCCCTGAAGGAAATCAGTGCTGGGCGTGTCTACATAAGTGCCAATCAGCAGCTTTGCTACCACCACTC TCTGAACTGGACCAGACTGCTTCGGGGGCCCTCGGAAGAGAGACTTGACATCAAGTACGACCGGCCTCTG GGAGAATGCTTGGCAGAGGGCAAAGTGTGTGATCCACTGTGTTCCTCTGGGGGATGCTGGGGCCCAGGCC CTGGTCAGTGCTTGTCTTGTCGAAACTACAGCCGGGAAGGTGTCTGTGTGACTCACTGCAACTTCCTGCA AGGGGAACCCCGGGAGTTTGTTCATGAGGCTCAATGCTTCTCCTGCCATCCAGAATGCCTACCCATGGAG GGCACCAGCACATGCAATGGCTCGGGCTCTGACGCTTGTGCACGATGTGCTCATTTTCGTGACGGGCCCC ACTGTGTGAACAGCTGCCCCCATGGAATCCTAGGTGCCAAAGGTCCAATCTACAAGTATCCAGATGCTCA GAATGAGTGTCGGCCCTGCCACGAGAACTGCACCCAAGGGTGTAATGGACCAGAACTACAAGACTGTTTA GGCCAAGCAGAGGTGTTGATGAGCAAACCTCATCTGGTAATAGCGGTGACGGTGGGACTGGCCGTGATCC TCATGATTCTGGGAGGCTCTTTTCTGTATTGGCGTGGACGCAGGATTCAGAACAAAAGGGCTATGAGACG CTACTTGGAGCGGGGTGAGAGCATCGAGCCTCTGGACCCAAGCGAGAAGGCAAACAAAGTCTTGGCTAGA ATCTTCAAAGAGACAGAGCTGAGGAAACTTAAAGTGCTTGGCTCTGGCGTCTTTGGAACTGTCCACAAGG GGATTTGGATTCCTGAGGGTGAATCCATCAAGATTCCAGTCTGCATTAAAGTCATCGAGGACAAGAGTGG GCGCCAAAGTTTTCAAGCTGTGACTGACCATATGCTGGCCGTTGGCAGCCTGGACCATGCCCACATTGTA CGGCTGCTAGGACTGTGCCCAGGGTCATCTCTGCAGCTTGTTACTCAGTACTTGCCCCTGGGCTCCCTCC TTGATCATGTGAAACAGCACCGTGAGACATTGGGACCACAGCTGCTGCTCAACTGGGGAGTACAAATTGC CAAGGGCATGTATTACCTCGAGGAACATAGCATGGTGCACAGGGACCTTGCGCTCCGGAACGTGATGCTC AAGTCACCAAGTCAGGTCCAGGTGGCAGATTTTGGTGTGGCTGACTTGCTGCCACCAGATGACAAGCAGT TGCTACATAGTGAGGCCAAGACTCCAATTAAGTGGATGGCCCTTGAGAGTATCCACTTTGGGAAATACAC ACACCAGAGTGATGTCTGGAGTTATGGTGTAACAGTTTGGGAGTTGATGACCTTCGGGGCAGAGCCCTAT GCAGGGTTACGACTGGCTGAAATACCAGACCTGCTAGAGAAGGGAGAGCGGTTAGCACAGCCCCAGATCT GCACCATTGACGTCTACATGGTCATGGTCAAGTGCTGGATGATTGATGAGAATATTCGCCCAACCTTTAA AGAACTAGCCAATGAGTTTACCAGGATGGCCCGTGACCCACCAAGGTATCTGGTCATCAAGAGAGCGAGT GGGCCTGGAACACCTCCTGCGGCAGAACCCTCTGTTCTGACCACCAAAGAGTTGCAGGAGGCAGAGCTGG AGCCAGAACTGGACCTAGACCTAGACTTGGAGGCAGAGGAGGAGGGCCTGGCCACCTCCCTGGGTTCTGC TCTCAGCTTGCCTACGGGAACGCTCACCCGGCCACGTGGGAGCCAGAGCCTTTTAAGTCCCTCATCTGGG TACATGCCCATGAACCAGAGCAGTCTCGGAGAGGCTTGTCTGGATTCTGCGGTTTTGGGGGGTCGTGAAC AGTTCTCCCGTCCCATCTCTCTGCACCCAATTCCACGGGGGCGTCCAGCATCAGAGTCATCAGAGGGCCA CGTGACAGGCTCTGAGGCTGAACTCCAAGAGAAAGTGTCAGTGTGTAGGAGCCGAAGCCGGAGTCGGAGC CCGCGGCCTCGTGGGGACAGTGCCTACCATTCGCAGCGACACAGCCTGCTTACTCCTGTGACCCCGCTCT CCCCACCAGGGTTAGAGGAGGAGGACGGCAATGGCTACGTCATGCCAGATACACACCTCAGAGGTGCGTC CTCCTCCCGGGAAGGCACCCTCTCGTCGGTAGGTCTCAGTTCTGTGTTGGGTACTGAGGAGGAAGATGAA GATGAGGAGTATGAATACATGAACCGGAAGAGAAGGGGTAGCCCTCCTCGGCCCCCCAGGCCTGGCTCCC TGGAAGAACTGGGTTACGAGTACATGGATGTGGGTTCAGACCTCAGTGCTTCTCTGGGCAGTACACAGAG CTGCCCGCTCCATCCCATGGCCATCGTGCCCTCTGCTGGCACAACTCCAGATGAAGACTATGAGTACATG AACCGGAGGCGTGGTGCGGGTGGCGCCGGAGGGGACTATGCAGCTATGGGGGCCTGCCCAGCAGCTGAGC AAGGGTACGAGGAGATGCGAGCTTTCCAGGGGCCTGGACATCACGCCCCCCATGTTCGTTATGCCCGCCT CAAAACTCTGCGTAGTTTAGAAGCCACCGACTCTGCCTTCGACAACCCCGATTACTGGCACAGCAGGCTT TTCCCCAAGGCTAATGCCCAGAGAACGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_017218
Insert Size	4020 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017218.2, NP_058914.2
RefSeq Size	4220 bp
RefSeq ORF	4020 bp
Locus ID	29496
UniProt ID	Q62799
Gene Summary	acts as a tyrosine kinase receptor for neuregulin; may play a role in axonal regeneration in response to injury [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.