Nfe2 (NM_001012224) Rat Untagged Clone
CAT#: RN210647
Nfe2 (untagged ORF) - Rat nuclear factor, erythroid derived 2 (Nfe2), (10 ug)
CNY 3,990.00
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN210647 representing NM_001012224
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCCGTGTCCTCCCCAGCCAAACAGAAACAGATTACCACAGCTGCCTACTGGGGAGCTTGGAGAGA TGGAACTGACTTGGCAAGAGATCATGTCCATTACTGAGCTGCAGGGTCTAAATGTTCCAAGTGAGCCATC TTTTGAGCCTCAAGCACCCACCCCATACCCTGGGCCACTGCCACCTCCAACATACTGCCCCTGTTCAATT CATCCAGATGCAGGCTTCACCCTTCCTCCACCACCTTATGAGCTCCCAGCATCCACTCCCCATGCTCCAG ACCTCCCATACTCCTATGGCAACATAGCCATACCCGTGTCAAAGCCACTTACCCTTTCAGGCCTGCTCAA TGAGCCCCTCCCAGACCCCTTAGCTCTCTTGGACATTGGGCTGCCAGTGGGGCAACCCAAGCCCCAAGAA GATCCAGAATCTGACTCGGGATTATCCCTCAACTACAGCGATGCAGAATCTCTTGAGCTAGAAGGTACAG AGGCTGGCAGGCGGAGGAGCGAGTATGTGGACATGTACCCGGTGGAGTACCCTTACTCACTTATGCCCAA CTCTTTGGCCCACCCCAACTATACTCTTCCGCCCACTGAAACACCCTTGGTCTTAGAGTCATCCTCCGGT CCAGTGCGGGCTAAGCCTGCTGTCCGTGGGGAGGCAGGGAGTCGGGATGAGCGGCGGGCCCTGGCCATGA AGATTCCTTTCCCTACGGACAAGATAGTTAACTTGCCGGTAGATGACTTTAATGAGTTGTTGGCACAGTA TCCGCTAACGGAGAGCCAGCTGGCTCTAGTTCGGGACATCCGTCGGCGGGGCAAGAACAAGGTGGCAGCC CAGAACTGTCGCAAGAGAAAACTGGAAACCATTGTGCAGTTGGAGCGAGAGCTGGAGCGGCTGGGCAGTG AAAGGGAGCGGCTCCTCAGAGCCAGAGGGGAGGCTGACCGCACTCTGGAGGTCATGCGCCAACAGCTGAC AGAGCTGTACCATGATATTTTCCAGCATCTTCGGGATGAATCTGGCAACAGTTATTCACCAGAGGAATAT GTACTGCAACAGGCTGCTGATGGGGCCATCTTTCTGGTACCCCGTGGGACCAAAATGGAGGCCACAGATT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001012224 |
Insert Size | 1122 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001012224.1, NP_001012224.1 |
RefSeq Size | 1684 bp |
RefSeq ORF | 1122 bp |
Locus ID | 366998 |
UniProt ID | Q6AYT2 |
Gene Summary | Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR210647 | Nfe2 (Myc-DDK-tagged ORF) - Rat nuclear factor, erythroid derived 2 (Nfe2), (10 ug) |
CNY 3,990.00 |
|
RR210647L3 | Lenti ORF clone of Nfe2 (Myc-DDK-tagged ORF) - Rat nuclear factor, erythroid derived 2 (Nfe2), (10 ug) |
CNY 6,080.00 |
|
RR210647L4 | Lenti ORF clone of Nfe2 (mGFP-tagged ORF) - Rat nuclear factor, erythroid derived 2 (Nfe2), (10 ug) |
CNY 6,650.00 |