Myo5b (NM_017083) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Myr6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210559 representing NM_017083 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTACAGCGAGCTCTACAGCCGGTACACGAGGGTCTGGATCCCCGACCCAGATGAGGTGTGGCGCT CGGCTGAACTAACCAAGGACTACAAAGATGGGGATGAGAGCCTACAACTCAGACTGGAAGATGACACAAT TCTGGATTACCCAATCGATGTCCAAAATAACCAGGTGCCATTCTTGCGGAATCCAGATATCTTAGTAGGA GAAAACGACCTCACTGCACTCAGCCATCTCCACGAGCCCGCAGTTCTGCATAACTTAAAGGTCCGTTTCC TGGAGTCTAACCACATCTACACTTACTGTGGAATTGTGCTTGTTGCCATTAATCCCTATGAGCAGCTGCC AATCTACGGGCAGGATGTCATCTATGCCTACAGTGGCCAAAATATGGGCGACATGGATCCCCACATCTTT GCTGTAGCAGAAGAAGCCTACAAGCAGATGGCCAGAGATGAAAAGAACCAATCCATCATAGTCAGCGGAG AGTCTGGAGCAGGGAAGACCGTGTCAGCCAAGTATGCCATGCGCTATTTTGCCACAGTTGGGGGCTCAGC CAGTGATACCAACATCGAAGAGAAGGTCCTGGCTTCCAGTCCCATCATGGAGGCCATCGGGAATGCCAAG ACAACTCGCAACGACAATAGCAGCCGATTTGGGAAGTACATTGAGATCGGCTTTGATAAAAAGTACCACA TCATCGGGGCCAACATGAGGACCTATTTGCTGGAGAAGTCCAGGGTGGTCTTTCAGGCGGATGATGAGAG GAACTACCACATCTTTTACCAGCTCTGTGCTGCGGCCAGCCTTCCTGAGTTTAAGGAGCTCGCACTAACA TGTGCAGAGGACTTTTTCTACACCGCCCATGGAGGAAACACGACCATCGAGGGTGTAGATGATGCAGAGG ACTTTGAGAAGACAAGACAAGCCCTCACACTCCTTGGAGTTCGGGAGTCCCATCAAATCAGCATCTTTAA GATAATTGCTTCTATCTTGCACCTTGGAAGTGTGGAGATCCAGGCCGAGCGGGATGGGGACTCCTGCAGT ATATCGCCCCAGGACGAACACCTGAGCAACTTCTGCCGCCTGCTAGGAATAGAGCACAGTCAGATGGAGC ACTGGCTGTGTCATCGGAAGCTGGTCACCACCTCCGAGACCTACGTCAAGACCATGTCCCTGCAGCAAGT GGTCAACGCACGCAATGCCTTGGCCAAGCACATCTATGCCCAGCTGTTCTCCTGGATTGTGGAGCACATC AACAAGGCCCTGCAAACCTCCCTTAAACAACACTCCTTCATCGGGGTCCTGGATATTTATGGGTTTGAGA CCTTCGAGATTAATAGCTTCGAGCAGTTTTGTATCAACTATGCCAACGAAAAGCTCCAGCAACAGTTCAA CTCGCATGTGTTCAAGCTGGAGCAAGAAGAGTATATGAAGGAGCAGATCCCGTGGACCTTGATTGACTTC TATGATAACCAACCGTGCATAGACCTCATAGAAGCCAAGCTGGGTATCCTGGACCTGTTGGATGAGGAGT GTAAGGTCCCCAAAGGAACTGATCAGAACTGGGCTCAGAAACTCTATGAACGGCACTCCAACAGTCAACA CTTCCAGAAACCACGCATGTCCAACACGGCCTTCATTGTCATCCACTTTGCAGACAAGGTGGAATACCTT TCAGATGGTTTTCTGGAGAAAAACAGGGACACGGTATATGAAGAACAGATCAACATCCTGAAAGCGAGCA AGTTTCCGCTAGTGGCTGATTTGTTCCGTGATGACGAGGACTCTGTTCCTGCCACCAACACAGCTAAGAG TCGGTCATCTTCAAAGATCAATGTTCGTTCTTCCAGACCCCTCATGAAGGCCCCCAACAAGGAGCACAAG AAATCCGTGGGCTACCAGTTCCGCACTTCCCTAAACCTGCTTATGGAGACTCTGAATGCCACAACGCCCC ACTACGTTCGATGCATCAAGCCCAACGACGAAAAGCTCCCCTTCCACTTCGACCCAAAGAGAGCTGTGCA GCAGCTCAGAGCCTGCGGCGTGTTGGAGACCATTCGGATCAGCGCGGCAGGCTACCCGTCCAGGTGGACC TACCATGACTTCTTCAACCGGTATCGGGTGTTGATGAAGAAGAGAGAGCTTGCCAACACCACCGACAAGA AGAATATCTGCAAGTCTGTCCTGGAGAGTCTCATCAAGGATCCAGACAAGTTCCAGTTTGGCCGCACCAA GATCTTCTTCCGGGCAGGTCAGGTGGCCTACCTGGAGAAGCTTCGGGCAGACAAGTTCCGGGAGGCCACC ATCATGATCCAAAAGACGGTCAGGGGCTGGCTGCAGAGAGTGAAGTACCGGAGGCTGAGAGCAGCTACGC TAACCCTGCAGAGATTCTGCCGAGGATACTTAGCCCGCAGACTGACTGAGCACTTGAGGAGAACCCGGGC GGCCATAGTGTTCCAGAAGCAGTACCGCATGCTGAAGGCCCGCCGAGCCTACTGCAGGGTCCGCAGGGCT GCGGTCATCATCCAGTCCTACACGAGGGGCCATGTGTGTACGCAGAAGCTACCGCCAGTCCTCACGGAGC ACAAAGCCACCATCATCCAGAAGTACGCCCGGGGCTGGATGGCACGGAGACATTTTCAGCGGCAGCGGGA TGCAGCCATTGTCATCCAGTGTGCCTTCCGGAGGCTCAAGGCCAGGCAGGCACTGAAGGCCCTCAAGATC GAGGCCCGTTCTGCAGAGCATCTGAAACGCCTCAACGTGGGCATGGAGAACAAAGTTGTCCAGCTGCAGC GGAAGATTGATGACCAGAACAAAGAGTTCAAGACTCTGTCAGAGCAGTTGTCTGCAGTTACCTCCACCCA TGCCATGGAGGTGGAGAAGCTGAAGAAGGAGCTGGCGCGTTACCAGCAGAACCAGGAGGCTGACCCCAGC CTTCAGCTGCAGGAGGAGGTACAGAGCCTGCGCACCGAACTACAGAAGGCTCATTCAGAGCGCAGGGTCC TAGAGGATGCTCACAACAGGGAGAATGGTGAACTGAGAAAGCGAGTCGCAGACCTGGAACATGAAAATGC ACTCTTGAAGGATGAGAAAGAACACCTTAACCACCAAATCCTGCGCCAGTCAAAAGCTGAATCTTCACAG AGCTCTGTGGAGGAAAACCTGCTGATTAAGAAGGAACTGGAGGAGGAACGGTCCCGGTACCAGAACCTCG TGAAGGAGTACTCCCAGCTGGAGCAGAGATATGAGAACCTTCGGGACGAGCAGCAAACTCCAGGCCACAG GAAGAACCCATCAAATCAAAGTAGCTTAGAATCTGACTCCAATTACCCCTCCATTTCCACCTCAGAAATC GGAGACACCGAGGATGCCCTACAGCAGGTGGAGGAGATTGGTATAGAGAAGGCAGCCATGGACATGACTG TCTTCTTGAAGCTGCAGAAGAGAGTGCGGGAACTTGAGCAGGAGAGGAAGAAGCTGCAGGTGCAGCTAGA AAAGGAACAACAGGACAGCAAGAAAGTGCAGGTAGAACAACAAAACAATGGCCTAGATGTGGACCAGGAT GCAGATATAGCCTACAATAGTCTGAAGAGACAAGAGCTCGAGTCAGAGAACAAGAAGCTGAAGAATGATC TGAATGAGCGCTGGAAGGCTGTAGCCGACCAAGCCATGCAGGATAACTCCACTCATAGCTCCCCCGACAG CTACAGCCTCCTACTGAACCAGCTCAAGCTGGCCAACGAGGAGCTGGAGGTCCGCAAAGAGGAGGTGCTG ATCCTCAGGACCCAGATCATGAATGCTGACCAGCGCAGACTGTCCGGCAAGAACATGGAGCCGAACATCA ATGCCAGAACAAGTTGGCCCAACAGCGAGAAGCACGTGGACCAGGAAGATGCCATTGAGGCCTATCACGG GGTCTGCCAGACAAACAGCCAGACTGAGGATTGGGGATATTTGAATGAAGATGGAGAACTCGGCTTGGCT TACCAAGGCCTAAAGCAAGTCGCCAGGTTGCTGGAGGCCCAGCTGCAGGCCCAGAACCTGAAGCATGAGG AGGAGGTGGAGCATCTCAAGGCCCAGGTGGAGGCCATGAAGGAGGAGATGGACAAACAGCAGCAGACCTT CTGCCAGACTCTGCTGCTCTCCCCAGAGGCCCAGGTAGAATTTGGTGTCCAGCAGGAGATATCCCGGCTG ACCAATGAGAACCTGGACTTTAAGGAGTTGGTGGAAAAGCTGGAGAAGAATGAGAAGAAGCTGAAAAAGC AGCTGAAGATTTACATGAAGAAGGTCCAGGACTTAGAAGCTGCCCAGGCATTGGCACAGAGTGACAGGAG GCACCATGAACTCACAAGACAGGTCACGGTCCAACGAAAAGAGAAGGACTTCCAAGGCATGCTGGAGTAC CACAAAGAGGACGAGGCACTCCTTATCCGGAACCTGGTGACAGACCTGAAGCCCCAGATGCTGTCGGGCA CCGTGCCCTGTCTGCCTGCATACATACTCTACATGTGCATCAGGCACGCGGACTACACCAACGATGACCT CAAGGTGCACTCGTTGCTGAGCTCCACCATCAATGGCATTAAGAAAGTCCTCAAGAAGCACAATGAGGAC TTTGAGATGACGTCATTCTGGTTATCCAACACCTGCCGCCTCCTTCACTGTTTGAAGCAGTACAGTGGGG ATGAGGGTTTCATGACACAGAACACGGCGAAGCAGAATGAGCACTGTCTCAAGAACTTTGACCTCACTGA ATACCGTCAGGTGCTAAGTGACCTTTCCATTCAGATCTATCAGCAACTCATTAAAATTGCTGAGGGCCTG CTACAGCCTATGATAGTTTCTGCCATGCTGGAAAATGAGAGTATCCAGGGGCTGTCAGGGGTGAGGCCAA CTGGCTACCGGAAGCGCTCGTCCAGCATGGTGGACGGAGAAAATTCGTATTGCCTGGAGGCCATCATCCG CCAGATGAATTTCTTTCATACAGTCCTGTGTGACCAGGGCCTGGACCCCGAGATTATCCTGCAGGTGTTC AAACAGCTTTTCTACATGATCAATGCGGTGACTCTTAACAACCTACTCCTGCGGAAAGATGCCTGCTCCT GGAGCACCGGCATGCAACTCAGGTACAACATAAGTCAACTCGAAGAGTGGCTTCGGGGAAAAAACCTTCA GCAGAGTGGAGCGGTTCAGACCATGGAGCCCTTGATCCAGGCAGCCCAACTCCTTCAGCTGAAGAAGAAG ACCCAGGAGGATGCTGAGGCCATCTGCTCTCTGTGTACCTCCCTCAGCACCCAGCAGATTGTCAAAATTT TAAACCTCTACACTCCCTTGAATGGATTTGAAGAACGGGTGACAGTGTCCTTTATACGAACAATCCAGGC TCAGCTACAAGAGAGGAGTGACCCCCAGCAGCTACTGCTGGACTCCAAACACATGTTTCCTGTTCTGTTT CCATTTAATCCATCTGCTCTGACCATGGACTCAATCCACATCCCGGCCTGTCTCAATCTGGAGTTCCTCA ATGAAGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017083
Insert Size	5541 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017083.1, NP_058779.1
RefSeq Size	6075 bp
RefSeq ORF	5541 bp
Locus ID	25132
UniProt ID	P70569
Gene Summary	3 sequence has high similarity to a putative mouse glutamic acid decarboxylase [RGD, Feb 2006]

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