Dot1l (NM_001108733) Rat Untagged Clone

CAT#: RN210536

Dot1l (untagged ORF) - Rat DOT1-like, histone H3 methyltransferase (S. cerevisiae) (Dot1l), (10 ug)



  "NM_001108733" in other vectors (1)

CNY 24,890.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN210536 representing NM_001108733
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGAGAAGCTGGAGCTGAGGCTCAAGTCGCCTGTGGGCGCGGAGCCCGCCGTCTACCCGTGGCCGC
TGCCGGTCTACGTGAGTGTACTTGCGGAGAACCCGGGGGACAAGCACCATGATGCTGCTCATGAGATTAT
CGAGACTATCCGATGGGTCTGCGAGGAAATCCCAGATCTCAAGCTGGCCATGGAGAACTATGTCCTGATC
GACTACGACACCAAAAGTTTTGAAAGCATGCAGAGGCTGTGTGACAAATATAACCGGGCCATTGACAGCA
TCCACCAGCTGTGGAAGGGCACTACACAGCCCATGAAGCTCAATACTCGGCCGTCCAATGGGCTCCTGCG
GCACATCCTGCAGCAGGTGTACAACCACTCTGTGACCGACCCAGAGAAGCTCAACAACTACGAGCCCTTC
TCCCCCGAGGTGTACGGGGAGACCTCCTTTGACCTGGTGGCCCAGATGATCGATGAGATCAAGATGACCG
AGGATGACCTGTTTGTAGACCTGGGCAGTGGTGTGGGGCAGGTTGTCCTTCAGGTTGCGGCGGCCACCAA
CTGCAAACATCACTACGGAGTGGAGAAAGCGGACATCCCAGCCAAGTACGCGGAGACCATGGATCGAGAG
TTCAGGAAGTGGATGAAATGGTATGGAAAAAAGCATGCAGAATACACACTGGAGCGAGGTGACTTCCTCT
CAGAGGAGTGGAGGGAACGGATCGCCAACACGAGTGTTATATTTGTGAATAACTTTGCCTTTGGTCCTGA
GGTGGATCACCAGCTGAAGGAACGATTCGCAAACATGAAGGAAGGCGGCAGAATCGTATCCTCAAAGCCC
TTTGCCCCTCTGAACTTCAGGATCAACAGCAGGAACTTGAGTGACATTGGCACCATCATGCGTGTGGTGG
AGCTGTCGCCCCTGAAGGGCTCTGTGTCATGGACTGGCAAGCCTGTCTCCTACTACCTGCACACCATTGA
CCGCACCATACTTGAAAACTATTTTTCTAGTCTGAAAAATCCAAAACTCAGGGAGGAGCAGGAGGCAGCT
AGGCGCAGGCAGCAGCGAGAGAACAAGAGCAACGCGACCACACCCACCAAAGTCCCTGAGAGCAAGGCAG
CTGCCACCGAGGCCCCTGCGGATTCTGGTGCTGAGGAAGAAAAATCAGGTGTGGCCACCGTCAAAAAGCA
ATCTCCCTCCAAAGCCCGGAAGAAGAAACTGAACAAGAAAGGGAGAAAAATGGCTGGCCGGAAGCGTGGG
CGACCCAAGAAAATGAGTGCTGCCAGTGCCGATCGCAAGTCCAAGAAGAGCCAAAGCACACTGGATCTCC
TGCACTCTCCGCCCCCAGCCCCACCCTCAGCCTCACCCCAGGATGCATACAGGGCCCCCCACAGCCCGTT
CTACCAGCTACCTCCGAGCACGCCGCTGCACTCGCCCGACCCACTGCTAGTGGCTCCCACCCCACCCGCG
CTGCAGAAGCTGTTAGAGTCCTTCAAGATCCAGTACCTGCAGTTCCTGGCCTACACGAAGACCCCGCAGT
ACAAGGCCAACCTGCAGCAGCTTCTGGACCAGGAGAAGGAGAAGAACACACAACTGCTGGGTACAGCCCA
GCAGCTCTTCAGCCATTGCCAGGCACAAAAGGAAGAGATCCGCAGGCTGTTCCAGCAGAAGCTGGATGAG
TTGGGCGTGAAGGCACTGACCTACAATGACCTGATTCAGGCCCAGAAGGAAATCTCTGCCCACAACCAGC
AGCTGCGGGAGCAGTCGGAACAGCTGGAGAAGGACAACAGCGAGCTTCGGAGCCAGAGCCTGCGGCTGCT
CAGGGCCCGGTGCGAAGAGCTGAGGCTTGACTGGTCCACCTTGTCCCTGGAGAACCTGCGGAAAGAGAAG
CAGGCCCTGCGGAGCCAGATCTCAGAGAAGCAGCGGCACTGCCTAGAGCTGCAGATCAGCATCGTGGAGC
TGGAGAAGACCCAGCGCCAGCAGGAGCTCCTGCAGTTGAAGTCCTGTGTGCCACCAGATGACGCTCTGTC
CCTGCACCTGCGTGGCAAGGGTGCCCTGGGCCGCGAGCTGGAGTCTGACGCTGGGCGCTTGCGCCTTGAG
CTGGACTGTGCCAAGCTCTCCCTCCCACACCTCAGCAGCATGAGCCCTGAGCTGTCCATGAATGGCCATG
CCGCCAGCTATGAGCTCTGCGGTGCAGCTAGTCGGCCCTCGTCCAAGCAGAACACCCCCCAGTATCTGGC
CTCCCCCTTGGACCAGGAGGTCGTGCCCTGCACCCCTAGCCACAGTGGCCGGCCTCGGCTGGAGAAGCTG
TCTGGCCTGGCTTTGCCTGACTACACCCGGTTGTCACCGGCCAAGATTGTGTTGAGGCGGCACCTGAGCC
AGGACCACACCGGGGCCAGCAAAGCAGCTACTAGTGAGCCACACCCTCGGCCAGAGCATGCCAAGGAGAA
CAGCCTTCCCTACCAGAGCCCTGGCTTGTCGAACAGCATGAAGCTCAGCCCCCAGGATCCACCGCTGGCC
TCCCCGGCAACCTCACCCCTCACTTCCGAGAGGGGTAGTGAGAAGGGTGTAAAGGAGCGTGCCTACAGCA
GCCATGGGGAGACCATCACCAGCCTGCCTGTCAGCATTCCACTCAGCACAGTACAGCCCAACAAGCTGCC
TGTCAGCATCCCGCTAGCCAGCGTGGTGCTGCCCAGCCGTGCCGAGAGGGCGAGGAGCACTCCCAGCCCT
GTGCCCCAGCCTCGAGACTCCTCATCCACACTTGAGAAGCAGATTGGTGCTTCCACCCATGGTGCAGGGG
GTGCTGCGGCAGGAAGCAGGAGCCTCACTGTGGCGCCCACAGGTTTCTATGCTGGTTCGGTGGCCATCAG
TGGGGCTCTGGCCAGCAGCCCAGCACCTCTGGCCTCTGGAATGGAATCTGCTGTTTTTGAGGAGTCCTCT
GGCCCCAGCAGCCTCTTTGGCACCATGGGGTCTCGCAGCACACCGCCACAACATCCACCTCTCCTGCCGC
AGTCTCGCAACTCGGGCCCTGCCTCTCCTGCCCACCAGCTCGCAGCCAGTCCCCGCCTGAGTGTGACCAC
CCAGGGCTCACTGCCAGACACCGGCAAAGGGGAGCTGCCTGCTGATCCTGCCTTCTCAGACCCAGAGAGT
GAAGCTAAGAGGAGGATTGTGTTCAGCATTTCAGCTGGTGCCAGCTCCAGGCAGTCGCCTTCCACCAGGC
ACAGCCCCTTGACCTCTGGCACCCGTGGGGATTGTGTTCAGAGCCATGGGCAGGACAGTCGTAAGCGTAG
CAGAAGGAAGCGTGCATCAGCTGGAACGCCTAGCCTCACCACGGGTGTGTCCCCCAAGCGCCGAGCTCTG
CCAACCGTCGCTGGCCTCTTTACACAGTCTTCGGGGTCGCCTCTCAATCTCACCTCCATGGTCAGCAACA
TCAACCAGCCCCTGGAGATCACAGCCATCTCATCCCCGGAGAGCTCACTGAAGAGCTCCCCCACACCCTA
CCAGGACCATGACCAGCCTCCAGTGCTCAGGAAGGAGCGGCCCCTGGGCCCAACTAATGGGGCCCACTAC
TCTCCGCTGACGTCAGATGAGGAGCCAGGCTCGGAGGACGAGCCCAGCAGTGCCCGAATTGAGAGAAAAA
TTGCAACAATCTCTTTAGAAAGCAAATCTCCCCCGAAGACACTGGAAAATGGTGGTGGCTTAGTGGGAAG
GAAGCCGGTCCCCTCAGGCGAGCCTGTCAACAGCAGCAAATGGAAGTCCACCTTCTCACCCATCTCCGAC
CTCGGCTTGGCCAAGGCCATGGACAGTCCGCTACAGGCCAGCTCTGCACTGAGCCACAGCCCCCTCTTCT
CTTTCCGGCCGTCCTTGGAGGAGCCTGCTGCTGAGGCCAAGCCCTCCACCCACCCAAGGAAAAGTTTTGC
GGGCTCTCTGGCTGCAGCTGAGGGGCCAAGCCCTGGCACCAACCCTCCCAACAGCCTGGCCTTCAGTGGG
GGCCTTGCTGCAGACCTCGGTTTACATAGCTTCAATGACGGTGCTTCCCTCTCCCACAAGGGCCCGGAGG
TGGCGGGCCTGAGCGCCTCCCTGAGCTTCCCATCCCAGAGGGGCAAGGACAGTACCACAGAGGCCAACCC
CTTCCTCAGCAGGCGGCAGCCGGAGGGCCTGGGTGGCCTGAAGGGTGAGGGCAATGCAAGCAAGGAGTCA
GGAGAGAGCCTGCCCCTGTGTGGGCCCTCAGACAAGGTCTCACTGCCACACGGCAGCAGGGCCAGCAAAG
GCCGTGACCGCGAGCTGGACTTCAAGGGCGGCCACAACCTCTTCATCTCTGCTGCAGCCGTGCCTCCAGG
TGGCCTCCTTGGTGGCCCTGGTCTTGTAACTGTGGCTTCCTCTGCGGGCAGTGCGACACCCTCTGCCCAG
GCTCCCCGGCCTTTCCTGAGCACCTTTGCCCCTGGGCCCCAGTTTACTCTGGGCCCCATGTCCCTGCAGG
CCAACCTGGGCTCTGTGGCCGGCTCCTCTGTGCTGCAGTCCTTGTTCAGCACTGTGCCAGCTGCTGCAGG
CCTGGTGCATGTGTCATCCACTGCGACCCGACTGACCAACTCACACACCATGGGCAGCTTCTCCTCTGGG
GTGGCCGGCGGAACCGTTGGAGGTAATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001108733
Insert Size 4650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001108733.1, NP_001102203.1
RefSeq Size 6015 bp
RefSeq ORF 4650 bp
Locus ID 362831
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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