Madd (NM_053585) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210404 representing NM_053585 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAAAAGAAGTTCTGCCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCATCCAAGCA GTGACAGTGTGGCTCAGACTCCTGAACTTCTGCGGAGGTACCCACTAGAGGACCACCCGGAGTTTCCCCT GCCCCCAGATGTGGTGTTCTTCTGCCAGCCAGAGGGATGTCTGAGCGTGCGGCAACGGCGCATGAGCCTC CGAGATGATACCTCTTTTGTCTTCACCCTAACCGATAAGGACACTGGAGTCACCCGTTATGGCATCTGTG TCAACTTCTACCGTTCCTTTCAAAAGCGAATGCCAAAGGAAAAGGCGGAAGGCGGAGCAGGACCCCGTGG GAAGGAAGGAGCTCATGCCCCCTGTGCCTCAGAAGAGGCCGCCACCGAGAGCTCAGAGAGTGGCTCAACC TTGCAGCCTCCTAGTGCTGACTCCACTCCTGACGTAAACCAGTCTCCTCGGGGCAAACGTAGGGCAAAAG CAGGCAACCGCTCCCGAAACAGTACCCTGACATCCCTGTGTGTGCTTAGCCACTACCCTTTCTTCTCTAC CTTCAGAGAGTGTCTGTATACTCTCAAACGTCTGGTAGACTGCTGTAGTGAACGGCTCCTAGGCAAGAAA CCGGGCATCCCTCGAGGTGTACAAAGGGACACCATGTGGCGAATCTTTACTGGATCACTGCTAGTGGAGG AGAAGTCCAGTGCCCTTCTGCATGACCTTCGAGAGATCGAGGCCTGGATCTATCGGTTGCTACGCTCCCC GGTGCCTGTCTCTGGGCAGAAGCGAGTGGACATTGAGGTCCTACCCCAGGAAGTGCAGCAGGCCCTGACA TTTGCTCTTCCAGATCCATCCCGGTTCACCCTAGTAGATTTCCCTCTTCACCTTCCCTTGGAACTTCTGG GTGTAGATGCTTGTCTTCAGGTGCTAACCTGCATCCTGTTGGAGCACAAGGTGGTGCTACAGTCTCGAGA CTACAACGCACTCTCCATGTCTGTGATGGCGTTTGTGGCAATGATCTACCCCCTGGAGTACATGTTCCCT GTCATCCCGCTGCTTCCCACCTGCATGGCTTCGGCAGAGCAGCTACTCTTGGCTCCAACCCCATATATCA TTGGAGTCCCTGCCAGCTTTTTCCTCTATAAGCTAGACTTCAAAATGCCTGATGACGTGTGGCTGGTGGA TCTGGACAGCAACAGGGTGATTGCCCCCACCAATGCAGAAGTGCTACCCATCCTGCCAGAACCGGAATCA TTAGAGCTGAAAAAACACTTAAAGCAGGCTCTAGCCAGCATGAGTCTCAACACCCAGCCCATTCTTAATC TGGAGAAATTCCATGAGGGCCAGGAGACACCACTTCTCTTGGGAAGGTTTTCTAATGACCTTCAGTCTAC ACCTTCCACTGAATTCAACCCACTCATTTATGGCAATGATGTAGATTCAGTCGATGTTGCAACGAGAGTG GCCATGGTTCGTTTCTTCAACTCTGCTAACGTGCTGCAGGGCTTTCAGATGCACACACGTACCCTGCGAC TCTTCCCCCGGCCCGTGGTTGCCTTCCAAGCTGGCTCCTTTCTGGCCTCACGCCCCCGGCAGACCCCCTT TGCTGAGAAACTGGCCAGGACTCAAGCCGTGGAGTACTTTGGAGAATGGATCCTGAACCCTTCTAACTAT GCCTTCCAGCGGATTCACAACAATACGTTTGATCCAGCCCTCATTGGTGACAAGCCTAAGTGGTACGCTC ACCAGCTGCAGCCCATTCACTATCGAGTCTATGACAGCAATTCTCAGCTGGCTGAGGCCCTGAGTGTGCC ACCGGAGCGTGATTCTGAGTCTGACCCCACTGATGACAGTGGCAGTGACAGCATGGACTATGATGACTCA AGCTCTTCTTACTCCTCCCTGGGGGACTTCGTCAGTGAAATGATGAAGTGTGACATCAATGGCGACACTC CCAATGTGGATCCTCTGACGCATGCGGCCCTGGGAGACGCCAGTGAAGTGGAGATTGATGAGCTGCAGCC CCAGAAGGAGGGAGAGGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAGAACCTTCCCCTGCGCTCCAGC TCCAGCACCACTGCCAGCAGTAGCCCTAGCACCATCGTCCACGGCGCCCACTCTGAACCTGCTGATTCTA CAGAGGTGGGAGATAAGGCAGCAACAGGCATCTCCAAGCCCCTCCCTCCTGTGCCTCCCAGCATTTGCAA ATCTACTGTGGACAGGAGACAGACAGAGACTGGAGAGGGGTCAGTGTGCCAGCGAACCTACGACCATCCA TATTTTGAGCCCCAATATGGCTCCCCCGCTGAGGAAGATGATGACGAGCAGGGAGAGAGCTACACGCCCC GGTTCAGCCAACATGCCAGTGGCAGTCGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAAGCTGGCCAG TGACTCAGACGCAGAGTCAGACTCCCGAGCAAGCTCTCCCAACTCCACCGTCTCTAACAACAGCACCGAG GGCTTCGGGGGCATCATGTCTTTTGCTAGCAGCCTGTATCGGAACCATAGTACAAGCTTCAGCCTTTCAA ACCTCACACTGCCCACCAAAGGTGCCCGAGAGAAGACTACACCATTCCCCAGCCTGAAAGGAAACAGGAG GGCCTTAGTGGACCAGAAATCATCTGTCATTAAACACAGCCCAACTGTGAAAAGAGAACCTCCATCACCG CAGGGCCGGTCCAGCAATTCTAGCGAGAACCAACAGTTCCTGAAGGAGGTGGTCCACAGTGTGCTGGATG GCCAGGGAGTCGGCTGGCTCAACATGAAAAAGGTGCGCCGGCTGCTGGAGAGTGAGCAGCTCCGAGTCTT TGTACTGAGCAAGCTGAGTCGTGCAGTGCAGTCAGAGGATGATGCCCGACAGGATGTCATCCAGGATGTG GAGATCAGTCGGAAGGTGTACAAGGGAATGCTAGACCTCCTCAAGTGCACAGTCCTCAGCCTCGAGCAGT CCTATGCCCATGCTGGTCTGGGTGGCATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCATTA CTATAGTAAAGAACCAGACAAACGCAAGAGAAGTCCAACAGAGAATGTAAATACCCCAGTTGGCAAGGAT CCTGGTCTGGCTGGGCGGGGGGACCCAAAGGCAATGGCTCAGCTAAGGGTCCCTCAGCTGGGTCCTCGGG CACCAAGTGCTACAGGAAGGGGTCCTAAAGAACTGGACACCAGAAGTTTAAAGGAAGAGAATTTTGTAGC GTCTGTGGGACCAGAGGTCATCAAGCCTGTCTTTGACCTGGGTGAGACTGAGGAGAAAAAGTCCCAGATC AGCGCAGACAGTGGTGTGAGCCTGGCATCTGCTTCCCAGAGGACTGATCAAGACTCTGTCATCGGTGTGA GTCCAGCTGTTATGATCCGAAGCTCAAGTCAGGATTCTGAAGTGAGTAACAGTTCAGGAGAGACCCTCGG AGCAGACAGTGACCTGAGCAGCAATGCAGGTGATGGGCCAGGAGGCGAAGGCAGTGCCCACTTGGCAAGT TCTCGGGCTACTCTGTCTGACAGTGAAATTGAAACCAATTCTGCTACAAGCACCATCTTTGGTAAAGCCC ATAGCTTGAAGCCAAAGGAGAAGCCGGCTAGTAGTCCAGTTCGCTCTTCCGAAGATGTAAGCCAGCGAGT CTATCTCTATGAGGGACTACTAGGAAGGGACAAAGGATCCATGTGGGACCAGTTAGAGGATGCTGCTATG GAGACCTTTTCTATAAGCAAAGAGCGGTCTACTTTATGGGACCAAATGCAGTTCTGGGAAGATGCGTTCC TAGATGCTGTGATGTTGGAAAGAGAAGGAATGGGTATGGACCAGGGCCCTCAGGAAATGATCGACAGGTA CCTGTCCCTAGGAGAGCATGACCGGAAGCGCCTGGAGGATGATGAAGATCGCTTACTGGCCACACTCTTA CACAACCTCATATCCTATATGCTCCTGATGAAGGTGAACAAGAACGACATCAGGAAGAAAGTACGGCGCC TAATGGGAAAGTCCCACGTTGGGCTGGTGTACAGTCAACAAATCAATGAGGTGCTCGATCAGCTGACCAA CCTGAATGGGCGTGATCTCTCCATCCGATCCAGTGGCAGCAGGCACATGAAGAAGCAGACGTTCGTTGTG CACGCAGGGACGGACACAAATGGAGATATCTTTTTCATGGAAGTGTGTGACGACTGTGTGGTCTTACGTA GTAACATCGGGACAGTGTACGAGCGCTGGTGGTATGAGAAGCTTATCAACATGACCTACTGTCCCAAGAC CAAGGTCCTGTGCTTATGGCGTAGAAATGGCTCTGAGACGCAGCTCAACAAGTTCTATACCAAGAAGTGT CGGGAGCTGTACTACTGTGTGAAGGACAGCATGGAGCGTGCGGCTGCCCGGCAACAAAGCATCAAACCCG GGCCTGAACTAGGTGGTGAGTTCCCTGTGCAGGACATGAAGACTGGAGAGGGCGGCTTGCTTCAGGTCAC CCTGGAAGGGATCAATCTCAAGTTCATGCATAACCAGGTTTTCATAGAGCTGAATCACATTAAAAAGTGC AATACAGTTCGAGGCGTCTTTGTCCTGGAAGAATTTGTTCCTGAAATTAAAGAAGTGGTGAGCCACAAGT ACAAGACTCCAATGGCCCACGAGATCTGCTATTCTGTGTTGTGTCTCTTCTCGTATGTGGCCGCAGTTCG TAGCAGTGAAGAAGACCTCCGAACCCCACCTCGGCCTGTCTCTAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053585
Insert Size	4809 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053585.1, NP_446037.1
RefSeq Size	5249 bp
RefSeq ORF	4809 bp
Locus ID	94193
UniProt ID	O08873
Gene Summary	GDP/GTP exchange protein (GEP) for Rab3 subfamily small G proteins; may play a role in intracellular vesicle trafficking [RGD, Feb 2006]

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