Eif4g3 (NM_001106693) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210289 representing NM_001106693 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCACAACCTCAGGCCCGGTCTCCGCCCAGCAGAGCAGTGCCAGTACACTGCCCAGACAACTGGA AGAGGAGGAAGGTTTTGGAACAGACTCCCGTTTACAGGTCCCTGGCTGGAAGAGGCTGGATAAAATACTG CATTTTTTTCCAGAGGCCTCAAATCCAGCCTCCCAGAGCGGCCATCCCAAACAGCAGTCCTTCCCTCCGT CCTGGTGCGCAGACCCCCACCGCCGTGTATCAGGCCAATCAGCACATCATGATGGTTAACCATCTGCCTA TGCCATACCCAGTGCCCCAGGGGCCTCAGTACTGCATACCACAGTACCGCCACAGTGGCCCTCCGTATGT GGGGCCCCCACAGCAGTACCCAGTTCAGCCACCCGGCCCCGGGCCTTTTTATCCTGGACCAGGACCTGGG GACTTTGCTAATGCTTATGGAACGCCTTTTTACCCAAGTCAGCCAGTGTATCAGTCAGCACCTATCATAG TGCCTACGCAGCAGCAGCCCCCTCCAGCCAAGAGAGAGAAAAAAACTATACGAATTCGAGATCCAAATCA GGGAGGTAAAGACATAACCGAGGAGATTATGTCTGGAGGTGGCAGCAGAAATCCCACTCCACCTATAGGA AGACCCACATCCACACCCACTCCTCCTCAGCAGCTGCCCAGCCAGGCCCCTGAGCACAGCCCTGTGGTAT ATGGGACTGTGGAGAGCGCTCATCTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGA AGAAAAGCCAAAACCAGACCCAGTGTTCCAGTCTCCTTCAACAGTCCTTAGGCTCGTGCTTAGTGGGGAG AAGAAAGAGCAAGCAGGCCAGATACCCGAGACTGCTGCAGGAGAGCCCAGCCCAGAGCCTCCTCGGACCT CATCACCCACCACCCTTCCTCCACTTGCCCGGAGTTCACTCCCTTCCCCCATGTCCGCTGCCCTTAGCAG CCAGCAGCTGCTCACTGCTGAGGACAAGTGTGAACTCTCTTCCTCAAAAGATGATGCCCCCCCTGTGCGC AGCCCCACGTCTTGCACAGCAGCACCAGGCCCTTCACTAACAGATGACATTGGTCTATGCAAGAAACCCT GTGGTGTAGCACCTCCTGACGGCCAGCTCGTTTCTAGTCCTGTCCTAATTAATGAAATGAACGGAGTTGG TGAGAAATTACCAGCCAAGGAGAACACTGTGGACATGGTGAGACAGGAAGTGTTGCCACTGACTCTTGAA CTGGAGATTCTGGAGCATCCCCAGGAAGAAAGGAAAGTGGAGTGTACCCCGACTCCCATCACCCCGTCCA CGATGCCTTCCTTCTCCCCAGCCCCTCCAACTCCGCCAGCTTCTCCTCCTTGTACTCCTGTTGTCCTGTC TGCCACCATTGCTCGATCTCCTGCTGTTGCCACTGTGATCCAGAGAGTTGTCGACGAGGGAGAGAGCCTG AGAACTTGTCTTAGCAAGGATGCCAAGGAGACACAGGACAAAGCGGAGGCAGAGTCAGATGGGCAAACAG AGGAGACTGCGGATCCCCAGAACGCACATGCAGGAAGGAGCCCTGCCCCAGTGCAAACAGCTACCACTGC GCCAAAGACGTGGAAGAAAACAAAAGAGCGGACCCGAACCCCAGATGAAGTGTTAGAAGCAGAGGCAGAG CCTAAAGCAGAAGAAGAGCTTTCAGTTGACAGTGTCCTTGAACCTGAGCAAGAGAAGATGAGCCAAGGGT TTCAGTTTGAGAGAGACCCCTCTACCCTGAAAAGAGCGAAAGCTGAGGAAGAAAACGGAGAAGAAGCAGA GCCTGTGCGCAATGGTGCTGAGAGTGCTTCCGAGGGGGAAGGAGGAGACGCTAATTCAGGCTCTACCGAT AGCTCTGGAGATGGGGTCACATTCCCATTTAAAGCAGAGTCCTGGAAACCTGCAGATACAGAAGGCAAGA AGCAATATGACCGGGAGTTCCTATTGGACATCCAGTTTATGCCTGCCTGTATCCAAAAACCAGAAGGCCT ACCTCCCATCAGCGATGTGGTTCTTGACAAGATCAACCAGCCCAGATTGCCAATGCGGACCCTGGATCCT CGAATTTTGCCTCGAGGACCTGATTTCACCCCAGCCTTTGCAGATTTTGGAAGACAGACGCCTGGTGGAA GAGGCGTGCCTTTGTTGAGTGTTGGACCTCGGAGGTCTCAACCTGGCCAGAGAAGGGAGCCCAGGAAGAT AATTACTGTCTCCGTAAAAGAAGACGTGCACTTGAGAAAGGCAGAGAATGCCTGGAAGCCCAGCCAGAAG CGGGACAGCCATGCCGATGACCCCGAAAGCATTAAAACGCAGGAACTTTTCAGAAAAGTTCGAAGTATCC TAAATAAACTGACACCACAGATGTTCAACCAGCTGATGAAGCAAGTGTCGGCACTTACCGTGGACACAGA GGAGCGGCTAAAGGGGGTCATCGACCTGGTCTTTGAGAAGGCTATCGATGAGCCCAGCTTCTCTGTGGCG TACGCAAACATGTGTCGGTGCCTAGTAACGCTGAAGGTACCTATGGCAGACAAGCCCGGTAACACAGTAA ATTTCCGGAAGCTGCTGCTTAATCGTTGCCAGAAGGAGTTTGAAAAGGATAAAGCAGATGATGACGTCTT TGAGAAGAAGCAGAAAGAACTGGAAGCTGCCAGTGCTCCAGAGGAAAGAACGAGGCTTCACGATGAACTG GAAGAAGCCAAGGACAAAGCCCGGCGGAGATCCATCGGCAACATCAAGTTCATTGGAGAGCTCTTTAAAC TCAAAATGCTGACGGAGGCCATCATGCATGATTGTGTGGTGAAGCTGCTAAAGAACCATGATGAGGAATC CCTGGAGTGCCTGTGTCGCCTGCTCACCACCATCGGCAAAGACCTGGACTTTGAGAAAGCAAAGCCGCGC ATGGACCAGTACTTTAACCAGATGGAGAAAATTGTCAAAGAAAGGAAAACTTCCTCCCGGATTCGGTTCA TGCTTCAGGATGTAATTGACCTCAGGCTGTGTAATTGGGTGTCCCGAAGAGCAGATCAGGGGCCAAAGAC TATCGAGCAGATTCACAAAGAGGCCAAAATAGAAGAACAAGAGGAGCAAAGGAAAGTCCAGCAGCTGATG ACCAAGGAGAAGAGAAGACCAGGCGTCCAAAGAGTGGATGAAGGTGGATGGAATACTGTACAAGGGGCCA AGAACAGTCGTGTACTGGACCCCTCCAAATTTTTGAAAATCACTAAGCCCACCATTGACGAGAAGATTCA GCTGGTTCCTAAGGCACAGCTGGGCAGCTGGGGCAAAGGCAGCAGTGGTGGAGCAAAGGCAAGCGAGACT GACGCCTTACGGTCAAGTGCTTCCAGTTTAAATAGATTCTCTCCTCTGCAACCTCCAGCGCCTTCAGGGT CCCCGTCAGCCACGCCTTTAGACTTCGATTCCCGGAGGGCGTTAACCAGTCGTGGGAGCATGGGCAGGGA GAAGAGCGACAAGCCGCTTCCAGCTGGAACAGCCCGTCCCAACACGTTCCTGAGGGGCAGCAGCAAAGAC CTGCTGGACAACCAGTCTCAGGAAGAGCAGCGCAGAGAGATGCTGGAGACGGTGAAGCAGCTGACAGGAG GCGTGGACGCCGAGCGGGCCAGCACTGAGGCCGACCGAAGCAAAGCGAGGGAGCTAGTCAAGTCAGAAGT GTGCACAGTGCCAGCCCCCGACAAGCCTGCACTGTCAGAAGAGGAAGTAGAGAGGAAGTCCAAGTCTATC ATTGACGAGTTCCTGCACATCAATGACTTTAAGGAGGCCACACAGTGCATAGAGGAGCTGAGTGCCCAGG GCCCACTGCATGTGTTCGTGAAGGTGGGTGTGGAGTTCACCCTGGAGCGGAGCCAGATCACCAGAGATCA CATGGGCCACTTACTGTATCAGCTGGTGCAGTCGGAAAAGCTCAGCAAGCAGGACTTTTTCAAAGGCTTT TCTGAAACCTTGGAGTTGGCAGATGACATGGCCATTGACATCCCCCATATTTGGTTGTACCTGGCTGAAC TAGTCACCCCCATGTTAAAAGAAGGGGGAATCTCCATGAGAGAACTTATTGTGGAATTCAGCAAGCCATT ACTTCCTGTTGGTAGAGCTGGGGTCCTGCTTTCCGAAATCCTGCACCTTCTATGCAGACAAATGAAGTTG GACTTCACGGAGTCAGAGGGACCCTGCTCCTCTGAAGCACTTTCAAAGAAAGAATTGTCTGCTGAGGAGC TGTCTCAGCGCCTGGAGAAACTGATCATGGAGGACAAAGCAGATGACGAGCGGATCTTTGACTGGGTGGA GGCCAATCTAGACGAAAGCCAGATGAGTTCGCCCACATTCCTTAGAGCTTTAATGACGGCCGTTTGCAAA GCAGCCATTGTCGCCGACTGTTCTACCTTCAGAGTGGACACTGCTGTGATCAAGCAGAGAGTGCCGATCT TACTCAAGTACCTGGACTCAGACACAGAGAAGGAGCTGCAAGCACTTTATGCACTACAAGCATCAATAGT GAAACTTGACCAACCTGCCAATTTGCTCCGGATGTTTTTTGATTGCCTGTATGATGAGGAGGTGATATCA GAGGATGCCTTCTACAAATGGGAGAGCAGCAAGGACCCTGCTGAACAAAACGGGAAGGGCGTAGCCCTCA AATCTGTCACAGCGTTTTTCACGTGGCTTCGGGAAGCAGAAGAGGAGTCGGAGGATAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106693
Insert Size	4752 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106693.1, NP_001100163.1
RefSeq Size	5307 bp
RefSeq ORF	4752 bp
Locus ID	298573

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