Gtf3c1 (NM_133541) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	TF3C-alpha; TFIIIC220
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210279 representing NM_133541 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCGCTCGAGTCGTTGTTGGACGAGGTGGCCCTGGAGGGGCTCGACGGCCTGTGCCTGCCCGCGC TGTGGAGCCGCCTGGAATCCCGCTCGCCTGCCTTCCCTTTGCCGCTGGAACCCTACACGCAGGAGTTTCT GTGGCGGGCCTTAGTCACGCACCCGGGCATCAGTTTCTACGAGGAGCCGCGAGAGCGACCTGACCTGCAG CTCCAAGACCGATATGAAGAAATCGACTTGGAAACTGGAATTTTGGAGTCCAGGAGGGACCCAGTCACTC TGGAAGATGTCTACCCCATTCATATGATCTTAGAGAATAAGGATGGCATCCAGGGCTCCTGCCGATACTT TAAGGAGAGAAAGGACATCACCAGTAGCATCAGGAGCAAGTGTTTGCAGCCTCGTTGTACAATGGTAGAA GCCTTCAGCAGGTGGGGTAAGAAGCTCATCATTGTGGCTTCCCAGGACATGCGATACCGGGCCTTGATAG GCCTAGAGGGGGATCCTGATCTGAAGCTTCCTGATTTCTCCTACTGCATTTTGGAGCGGTTAGGTCGATC GAGATGGCAAGGGGAGCTGCAACGAGACCTTCACACCACCGCTTTCAAGGTCGACGCTGGGAAGCTTCAT TATCACAGGAAAATTTTGAACAAGAATGGGCTCATTACAATGCAGTCACACGTGATCCGACTGCCTACTG GAGCCCAGCAACACTCCATCCTCCTCCTCCTTAACCGGTTTCATGTAGACAGGAGGAGCAAGTACGACAT CCTCATGGAGAAGCTGTCTATGATGCTGAGCACACGGAGCAACCAGATCGAGACTCTGGGCAAGCTGAGG GAGGAGCTGGGACTGTGTGAAAGGACGTTCAAGCGTCTGTACCAGTACATGCTGAATGCTGGGCTGGCCA AAGTGGTGTCCCTTCCCTTGCAGGAGATTCACCCTGAGTGTGGTCCCTGTAAGACCAAGAAAGGGACTGA TGTCATGGTTCGGTGCCTTAAGCTCCTGAAGGAGTTCAGGCGAAAGATGGAAGACGACCATGACGACGAT GACGATGAAGAGGCCATCTCCAAGGCTGTACCTCCCGTGGACATCGTGTTTGAGCGGGACATGCTCACGC AGACCTACGAGCTCATTGAGCGCAGAGGAACGAAAGGAATTTCCCAAGCTGAGATCCGAGTTGCTATGAA TGTGGGAAAACTGGAAGCAAGAATGCTGTGTCGGCTCCTTCAGAGGTTCAAGGTTGTCAAGGGATTCATG GAAGATGAAGGCCGGCAGAGGACCACCAAGTACATTTCCTGTGTATTTGCGGAGGAGAGTGACTTGAGCC GCCAATATGCGCGTGAAAAGGCCCGAGGAGAGCTGCTAACCACTGTGAGCCTCGCCTCAGTGCAAGATGA ATCCCTCATGCCTGAGGGTGAAGAGGCCTTCCTCTCTGACTCCGAAAGTGAGGAAGAGAGCAGCTGCAGT GGCAAGCGTAGGGGCCGAGGGTCCCGGGGGCATTCAAGAGCCTCTGGGGATGCTGGGCCTGGCTCTCGGC CTCATCACTCCACTCCAGCCAAGGGCGGGTGGAAAGTCCTAAATCTGCACCCATTAAAGAAGCCCAAAAG TGCCGCTGTGGAAAGATCCCGCCGAAGCTCTGCTTGTAGGGATGGCCTGGACACCAGCAGCTCATCCGAG CTCAATATACCCTTTGACCCTCACAGCATGGACAGTCACAGTGGTGACATCGCTGTGATTGAGGAGGTCC GGCTTGATAACCCCAAGGAGGGTGGTGGCTCCCAGAAGGGCGGACGGCATGGCTCAGGCCAGGACAAGCC TCACAAAACATACCGACTGCTAAAGCGCAGAAATCTGATTATAGAAGCTGTCACCAACCTCCGCCTGATT GAGAGCCTGTTCACGATTCAGAAGATGATCATGGACCAGGAGAAGCAAGAGGGTGTCTCCACCAAATGCT GTAAGAAGTCCATTATCCGCTTGGTGCGGAACCTGTCTGAGGAGGGTCTCTTGAGATTGTATAGGACTAC AGTCATTCAAGATGGCATCAAGAAGAAGGTAGACCTGGTCGTGCACCCTTCCATGGACCAGAATGACCCT CTTGTGAGAAGTGCTATTGAGCAGGTTCGCTTCCGGATCTCCAACTCAAGCACAGCAAACAGGGTTAAAG TTCCACCGGCCCCAGCACCCCAAGAGGAAGCAGAAGAGGGAAACCAAGAACCAGAGGTCCCAAGTAGATC AGCAGACTCTGAAGCGAACACATCCTCGAAGCCAGAAAGCACACGGGTGAAAAAGACAGATGAGAAAATG GGCATAACCCCACTCAAGAATTACAAACCTGTCATAGTTCCTGGACTTGGGCGCTCCATAGGGTTCCTTC CCAAGATGCCACGCCTTCGGGTGATGCACCTGTTCCTGTGGTACCTGGTGTATGGGCATCCTGCCAGCCA CACTGGGGAACAGCCTACTTTCCACAGTGAGAGGAAAACAGGCAAGCAGGAGCCCAGCAGACCAGGGGTC CAGCCCTCCTCAGGAGATGACTGGGACTCCTCTGAAGCCAAAAACAGCACAGAAAGCAGCTCCTGGGAGG CAGAAATGGAACTCTCCACAGAGAGAGTGTATGTGGATGAGATCTCATGGATGCGCTACGTCCCTCCCAT CCCCATCCACAGAGACTTCGGCTTTGGCTGGGCTCTTGTCAGTGACATTCTTCTCTGCCTTCCCCTCTCC ATCTTTGTCCAAGTTGTTCAAGTCAGCTACAAGGTGGATAACCTGGAGGATTTTCTGAATGACCCTCTGA AGAAGCACACACTGATCCGCTTCCTCCCTAGGCACATCCGGCAGCAGCTACTGTATAAGAGGCGGTATAT TTTCTCGGTGGTGGAGAACCTGCAACGGCTGTGCTACATGGGGCTGCTACAGTTTGGACCCACAGAGAAG TTTCAGGATAAAGACCAGGTCTTTGTTTTCCTGAAGAAGAATGCAGTCATTGTGGACACCACCATCTGTG ACCCACATTACAACCTGGCCCACAGCAGCCGGCCTTTTGAGAGACGTCTCTACGTCTTGGATTCCATGCA GGATGTGGAAAGCTACTGGTTTGACCTGCAGTGTATCTGCCTAAACACCCCACTAGGTGTGGTGCGTTGT CCATGTGCACAGAAGATCTGCCCTGATCCGGGCAGTGACCCAGAAGGCAGCTTGCGGAAGGAGCAAGAGA GCGCCATGGACAAGCACAACCTAGAGCGCAAGTGTGCCATGCTGGAGTATACCACGGGGAGCCGAGAAGT TGTGGATGAAGGCTTAGTCCCTGGGGATGGACTGGGAGCTGCTGGGCTGGACTCTAGCTTCTATGCACAC CTGAAGCGCAACTGGGTCTGGACCAGCTATATCATCAACAAAGCCAGAAAGAACAACACCTCCGAAAATG GGCTCACAGGAAGACTCCAGACTTTCCTGTCTAAGCGCCCAATGCCACTTGGGTCTGGAGGCAGTGGCCG TCTGCCTCTCTGGTCAGAGGGGAAAGCTGATGCTGAGCTCTGTGCTGACAAAGAGGAACACTTTGAACTG GACCGAGAACCTACCCCTGGCAGGAACCGAAAAGTGCGTGGGGGCAAGAGTCAGAAGCGGAAGCGGCTGA AGAAGGAGCCCATCAGGAAGACCAAGCGAAGGAGAAGAGGAGAGCATCCAGAAGCCAAAAGCAAAAAACT ACGCTACCAAGATGAGGCTGACCAGAACGCCCTCCGGATGATGACCCGGCTTCGGGTTTCTTGGTCCATG CAGGAGGATGGGTTACTAATGCTCTGCCGAATTGCCAGCAATGTCCTCAACACCAAGGTGAAAGGACCAT TTGTCACCTGGCAGGTCGTGCGTGACATTTTGCATGCTACCTTTGAAGAGTCTTTGGATAAAACATCTCA CTCGGTTGGACGAAGAGCTCGCTACATAGTCAAAAATCCCCAGGCCTTTATGAACTACAAAGTCTGCCTG GCAGAAGTGTACCAAGATAAGGCACTCGTTGGAGATTTCATGAGTCGGAAGGATAACTACGAAGACCCAA AGGTCTGTGCCAAGGAGTTTAAAGAATTTGTGGAGAAGCTGAAAGAGAAGTTCAGCTCTGGCCTGAGGAA CCCCAACCTTGAGATCCCAGACACACTGCAGGAACTCTTTGCCAAATACCGGGTGTTGGCAATCGGAGAT GAAAAGGACCGTGTCAGGAAGGAGGATGAACTGAATAGTGTGGAGGACATCCACTTCCTGGTGCTACAGA ACCTGATCCAGAGCACACTGTCCCTCTCTAACAGCCAGTCCAACTCTTGCCAGTCCTTCCAGATCTTCCG CCTCTACCGGGAGTTCCGGGAGCCTGTGCTGGTGCGGGCCTTCATGGAGTGCCAAAAGAGGAGCCTGGTC AACCGACGCCGTGTCAGCCACTCCCAGGGTCCCAAGAAGAACCGTGCTGTGCCCTTTGTGCCCATGTCCT ACCAGCTCTCCCAGTCATACTACAAGCTTTTCACCTGGCGATTTCCCACCACCGTCTGCACCGAATCCTT CCAGTTCTATGACAGACTACGGGCCAACGGCATACTAGACCAGCCTGATCATTTTTCTTTCAAGGACATG GATAGCAATGACCCTTCAAGTGACCTGGTGGCGTTTTCTTTGGACAGCCCTGGAGGACACTGTGTGACAG CCCTGGCCCTCTTCTCCTTGGGCCTCCTCTCTGTGGATGTCCGAATCCCAGAGCAGATTGTGGTGGTAGA CAGCTCCATGGTGGAGAGTGAGGTCATGAAGAGCTTGGGGAAAGATGGCGGCTTGGATGATGATGATGAG GAGGAAGACCTGGATGAAGGTTCAGGGACCAAGCGTCAGAGTGTGGAAGTAAAGGCCCATCAGGCCTCAC ACACCAAGTACCTGCTGATGAGAGGTTACTACACAGTGCCTGGCATGGTCAGCACGCGCAACCTGAACCC CAATGACAGCATTGTGGTCAATTCCTGCCAGGTTAAATTCCGACTGCGCAATACCCCTGCACCCACCCAT CTTGGGCCCACTGGGCCCACAGCCACACCGCTAGAAGAGCTCCAGGCTGGCCCTTCCTGCCTCCCTGCCT CCTTTACCAGCCTGGTGGATCCACAGCTGCACACCCGCTGCCCAGAAGAGTTTGCCCACCAGATGGCACA GTCTGGGTACAGCCCTGAGGACGTGGCTGCGTCCCTAGAGATCCTGCAGGCTGTGGCAGCTGCTGACTGC TTCGGGGTTGACAGGGAGAAGCTGAGCAGACAGTTCTCTGCCTTGGAGAAGATAGCGGACAAGCGCACCA GGACGTTCCTGGACTATATCCAGGACCTCTTGGAACAGCAACAAGTCATGGAAGTTGGAGGCAACACTGT GCGCCTAGTGGCCATGGCCTCTGCCCAGCCCTGGCTCCTGCCTTCAGTGCGTCTGAAAGATGTGGAAATA GACACCAAGGCCTCAGGGGATGACAGCCAATCTAGACTCCCTGCGGGGTCTTCCATCGAAGACCACACCT CTGAGGGTGCACCAATTCCTCCAGTCAGCTCCAATGGCACTAAAAAGCGCCCCTACTGCTCTATTCAGAG CCCTGAAACTGATGCTGAGGAAGCTACAAGGCTCCCTGCCAAGAAGCCCACATTACAAGATGTCTGTGTG GCTGCCAGTCCTAGGCCTGGAACAGAAGAGCAGACAGAGGCCCAGGCTCAGTTTGCAGCTCCTGAAGATG CAGGTGCAGAGGGCCCCAGGCAGGAGAGCCAAGAGAGTGTTGGTGTCTCAGGCCTAGAGCAGCTGGGCTG TGAGTTCCAGCTTCCAGAAAATTCTGAAGACCCCAGAGGACTTACAGAGAGCAACATGGCCCAGGTAGCA TGGGAGAGCGGCTGTGAAAGAGTATGTTTTGTCGGTCGCCCATGGCGTGGTGTGGACGGGCGCCTGAACA TGCCCGTGTGCAAAGGCATGATGGAAGCCGTGCTGTACCACATCATGTCAAGGCCCGGTGTTCCTGAGAG CTGCCTGCTACAGTACTACCAGGGCGTCCTACAGCCTGTTGCTGTGCTTGAGCTGCTCCGGGGCTTAGAG TCTCTCGGCTGTATCCAGAAGCGGATGCTGAAGAAGCCAGCGAGTGTCTCACTCTTCTCCAGGCCTGTGG TGGAGGGCCTAGGTCAGGCCAGTGAGGCCGAGGCACTCTCCTGTCAGGGGAGCACTGTCACCTTTTATGA ACCTACTCTGGACTGTACCATCCGTCTGGGCCGTGTCTTCCCCCATGACATCAACTGGAACAAGTGGATC CATTTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133541
Insert Size	6309 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133541.2, NP_598225.2
RefSeq Size	6905 bp
RefSeq ORF	6309 bp
Locus ID	171063
Gene Summary	subunit of general transcription factor IIIC, which is required for assembly of a preinitiation complex on 5S RNA, tRNA, and adenovirus VA RNA genes [RGD, Feb 2006]

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