Plcb4 (NM_024353) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Beta4aa; RATBETA4AA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210250 representing NM_024353 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAACCTTACGAATTTAACTGGCAGAAGGAGGTACCCTCTTTCTTGCAAGAAGGAGCAGTTTTTG ACAGATATGAAGAGGAATCTTTCGTGTTTGAGCCCAACTGCCTCTTCAAAGTGGATGAATTCGGCTTCTT CCTGACCTGGAAGAGTGAAGGCAAGGAAGGACAAGTGCTAGAATGTTCCCTCATCAACAGCATTCGGCTG GCAGCCATACCAAAGGATCCCAAAATCCTGGCTGCTCTCGAGTCTGTTGGAAAATCTGAAAATGATCTGG AAGGGCGGATATTGTGTGTCTGCAGCGGCACGGATCTGGTGAATATTGGCTTCACTTACATGGTGGCTGA AAACCCAGAAATAACTAAGCAATGGGTAGAAGGCCTGAGATCAATCATTCACAACTTCAGGGCAAACAAC GTCAGTCCGATGACATGCCTCAAGAAACACTGGATGAAACTGGCCTTTCTGACCAACACAAGTGGCAAAA TCCCAGTGAGGAGTATTACTAGAACCTTCGCATCAGGGAAAACAGAAAAGGTGATCTTTCAAGCACTCAA GGAATTAGGTCTCCCCAGTGGAAAGAATGATGAAATTGAACCTGCTGCATTTACCTATGAAAAGTTCTAT GAACTGACACAGAAGATTTGTCCTCGGACAGATATAGAAGATCTTTTTAAAAAAATCAATGGAGACAAAA CTGATTATTTAACGGTAGACCAATTAGTGAGCTTTCTAAACGAACATCAGCGAGATCCTCGGCTGAATGA AATTTTATTCCCGTTTTATGATGCTAAAAGAGCAATGCAGATCATTGAGATGTATGAACCTGATGAAGAG TTGAAGAAAAAAGGCCTCATATCCAGTGATGGATTCTGCAGATATCTCATGTCAGATGAAAATGCCCCCG TCTTCCTAGATCGCTTAGAACTTTACCAGGAGATGGACCACCCCCTGGCCCATTACTTCATCAGTTCCTC TCACAACACCTATCTCACCGGCCGGCAGTTTGGCGGAAAGTCTTCAGTGGAAATGTACCGACAAGTCCTC CTGGCTGGTTGCAGGTGTGTGGAACTTGACTGTTGGGATGGAAAAGGTGAAGATCAGGAACCAATAATAA CTCACGGAAAAGCAATGTGTACAGACATCCTTTTTAAGGATGTAATCCAGGCCATCAAGGAAACAGCGTT TGTCACATCAGAATATCCTGTAATTCTCTCCTTTGAAAACCACTGCAGCAAATATCAACAGTACCAGATG TCCAAGTATTGTGAAGATCTATTTGGGGATCTTCTGTTGAAACAAGCACTTGAGTCACATCCACTTGAAC CAGGGAGGCTCTTGCCATCTCCTAATGACCTCAAAAGAAAAATACTCATCAAAAATAAAAGGCTGAAGCC TGAAGTTGAAAAAAAACAGCTGGAGGCTTTGAAAAGCATGATGGAGGCTGGAGAATCAGCTGCCCCGGCC AGCATCTTGGAAGATGACAATGAGGAGGAAATAGAAAGCGCAGCTGATCAAGAGGAAGAAGCCCACCCTG AATACAAGTTTGGAAATGAACTTTCTGCCGATGACTTCAGTCACAAGGAAGCGGTTGCAAACAGCGTCAA GAAGGGCCTGGTCACCGTAGAAGATGAGCAGGCATGGATGGCATCTTATAAATACGTAGGTGCTACCACG AATATCCATCCGTACTTGTCCACGATGATCAACTATGCACAGCCTGTGAAGTTTCAAGGTTTCCATGTGG CTGAAGAGCGCAACATTCATTATAACATGTCTTCTTTTAACGAGTCGGTTGGCCTTGGCTACTTGAAGAC GCACGCGATTGAGTTTGTAAATTACAATAAGCGACAAATGAGCCGCATTTACCCCAAGGGAGGGCGAGTT GATTCCAGTAATTACATGCCTCAGATTTTCTGGAACGCTGGTTGCCAGATGGTTTCACTGAACTATCAAA CCCCAGATTTAGCGATGCAATTGAATCAAGGAAAATTTGAGTATAACGGATCCTGCGGATACCTCCTCAA GCCAGACTTCATGAGACGGCCTGATCGGACATTTGATCCCTTCTCTGAAACCCCTGTGGACGGGGTTATT GCAGCCACATGCTCAGTGCAGGTTATATCAGGGCAGTTCCTGTCAGATAAGAAGATCGGCACATACGTGG AAGTCGATATGTACGGGCTGCCCACCGACACCATACGGAAGGAGTTCCGAACCCGCATGGTTATGAACAA TGGACTCAACCCAGTTTACAATGAAGAATCGTTCGTGTTTCGGAAGGTGATCCTGCCTGACCTGGCTGTC CTGAGAATCGCTGTGTACGACGACAACAACAAGCTCATTGGCCAGAGGATCCTTCCTCTGGATGGCCTCC AGGCAGGTTACCGACACATCTCCCTGAGAAATGAGGGAAACAAACCGTTATCACTGCCAACAATTTTCTG CAATATCGTTCTTAAAACATATGTGCCTGATGGATTGGGAGATATTGTGGATGCTCTATCCGATCCAAAG AAATTCCTTTCAATCACAGAGAAGAGAGCAGACCAATTGAGAGCAATGGGCATTGAAACTAGTGACATAG CAGATGTGCCCAGTGACACTTCCAAAAATGACAAGAAAGGCAAGGCCAACCCAGCCAAAGCGAATGTAAC CCCTCAGAGCAGCTCTGAGCTCAGACCAACCACCACAGCTGCCCTGGGATCTGGCCAGGAAGCCAAGAAA GGTATTGAACTTATCCCTCAAGTGAGGATAGAAGATTTAAAGCAAATGAAGGCTTACTTGAAGCATTTAA AGAAACAACAAAAGGAGTTAAACTCTTTAAAGAAGAAACACGCAAAGGAGCACAGTACCATGCAGAAGTT ACACTGCACACAAGTGGACAAAATCGTGGCCCAGTATGACAAGGAGAAGTCGACTCATGAGAAAATCCTA GAGAAGGCCATGAAGAAGAAGGGGGGAAGTAATTGCCTTGAAATAAAAAAAGAAACAGAAATTAAGATTC AGACCCTGACATCGGATCACAAATCTAAGGTCAAGGAGATTGTGGCGCAGCACACAAAGGAGTGGTCAGA AATGATCAACACGCACAGCGCCGAGGAGCAGGAAATCCGGGACCTGCACCTGAGCCAGCAGTGTGAGCTG CTGAGGAAGCTGCTCATCAACGCTCATGAGCAGCAGACCCAGCAGCTGAAGCTCTCCCATGACAGGGAAA GCAAAGAGATGCGAGCCCATCAGGCTAAGATTTCTATGGAAAATAGCAAAGCCATCAGTCAAGATAAATC TATCAAGAATAAGGCAGAACGGGAGAGGCGAGTCAGGGAGTTGAACAGCAGCAACACTAAGAAGTTTCTA GAAGAAAGAAAGAGACTGGCAATGAAGCAGTCGAAAGAAATGGATCAGCTGAAAAAGGTCCAGCTGGAGC ACCTAGAATTCCTAGAGAAACAGAATGAGCAGGCGAAGGAGATGCAGCAGATGGTGAAATTGGAAGCCGA GATGGACCGCAGACCAGCAACAGTAGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_024353
Insert Size	3531 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024353.1, NP_077329.1
RefSeq Size	5297 bp
RefSeq ORF	3531 bp
Locus ID	25031
UniProt ID	Q9QW07
Gene Summary	phospholipase C isozyme; may play a role in phototransduction [RGD, Feb 2006]

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