Kank1 (NM_001037197) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ankrd15
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209886 representing NM_001037197 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTATACCACAAAAGTTAATGGCTGTGCTCCAGAAAACGCAGATGGAGTTCTGAATGGAGACCGTA ACAAGGAAAAGAAAGACCCGTATTTTGTGGAAACCCCGTACGGTTTTCAGCTAGACTTAGATTTCGTCAA GTACGTGGATGACATACAGAAGGGAAACACCATCAAGAAACTAAACATCCAGAAGAGGCGAAAGCCATCC GGGCCATGTGCAGAAGTCAGGGCCATACCCGGTCCTCAAGGTGTGTGGACTTCCACTGAGTCCCTGTCAT CCTCCAACAGTGATGACAGCAAGCAGTGTCCCAGCTTCCTCCTAGCCAGAAGCCACGTCACGTCGACCCC AATCCCACGGCCACCTGCCCCGCTAGAGACCTCGCCCACTTTTGCTATCTCAGAAAACCGACAGCTGCTG CCCCCTCCCTCGCCCCAACTCCCCAGGCACAACCTCCATGTCACCAAGACGTTGATGGAGACCCGGAGAA GGCTTGAACAGGAGAGAGTCACCATGCAGATGGCACCGGGTGAGTTCCGAAGGCCCAGGCTGGCCAGTTT TGGAGGCATGGGCTCCACGAGCTCCCTCCCATCCTTCATGGGTTCTGGCAACCACAGTTCTGCAATGCAC CAGCTTCAGAATGGATACCAAGGCAATGGCGATTATAGCAGCTATGTCCCAGCGGTGCCTACGACTTCTT CCATGGGAAGCTCCGTCCGTCACAGCCCGCTGAGTTCAGGGATCTCCACCCCGGTGACCAACGTGAGCCC CATGCACCTGCAGCACATCCGCGAGCAGATGGCCATTGCCTTAAAGCGCCTGAAGGAGCTTGAGGAGCAG GTGCGAACCATCCCTGTGCTCCAGGTCAAGATCTCGGTCTTGCAAGAAGAGAAAAGGCAATTGGCCTCGC AGCTGAAGAACCAGAGGGCCGCGTCCCAGAACGAGGCGTGTGGTGTGAGGAAGCGCTCCTACAGCGCAGG CAATGCCTCCCAGCTGGAATTGCTTTCCCGAGCCCGAAGAGGTGGCGGGGAATTATACATCGACTACGAG GAGGAAGAAATGGAGAGCGTGGAGCAGAGCACACAGAGGATCAGGGAGTTCCGGCAGCTCACGGCTGACA TGCAGGCGCTGGAGCAGAAGATTCAGGATAGCAGCTGTGAAGCCACGCCGGAGCTCAGGGAGAACGGGCA GTGCGCGTCTCGGGAGCGCAAGTCTGTGGCGGTGGGCAGCGATGAGAACATGAATGACATCGTTGTATAC CACAGGGACTTCAGGTCCCGCAAGGATACAGCTGTGGGGACAGTCACTGAGACGAGGAATTTTGGCATCA GCGTGACAGAAGCCATGCTTGGGGTTATTACTGAGGCTGACAAAGAGATCGAACTGCAGCAGCAGACCAT AGAGGCCTTAAAGGAGAAGATTTACCGCCTGGAAGTACAGCTTAAAGAAACCACCCATGACCAGGAGATG ACTAAGCTCAAGCAGGAACTACAGGCTGCCGGATCCAGGAAAAAAGTTGACAAGGCCATAATGGCCCAGC CACTTGTCTTCAGCAAGTTGGTGGAGGCGCTAGTGCCAACCAGAGAACAAATGGTCGGCAGTCACGTGGA CACAATGGATTCGTGTGTTGGGACCTCCGTGCAAACTAGTAGCGTAGGCACCTCCTGCCATCCTGACCGC AAGAACCAAGTCGTGGGGCCCGAGCTGCCTATGAATTGGTGGGTCGTAAAGGAGAGGGTGGGGACTCACG ACCGATGCGTAGGGAGGTCTGTTGAGACTTGTAACCGGAGTGTGGGTGTAGAAGTCAGTGTCTGTGAAAC AGGCAGCAACACAGAGGCTTCTGGGAGCGACCTGACCCTCCTGAAGACAAACTTGAACCTCAAAGACGTG CGGTCCATTGGCTGTGGAGACTGTTCTGTTGATGTGATTGTCTGCTTGCCCAAGGAGTGCACCTCCCGAA GCATGAACACAGAGGCTGTAGGTCAGGGGGAAGCTGCTGTCATGGCGGTGCCCCATACCACAGACCAGCA CACCAGCACGAATCTGGAGCGGGTGGACCAGGGCACTAACACGGAGGTAACCACCCTGGTGGAGTCCTGC ACCAACACTCTCCTCAGCACTCTGGACAAGCAGACCAGCACCCAGAATGTGGAGATGCGAACGGTGGCCA TCGGAGAAGGCCGAGTCAGAGACATCAACCCCTCCACAAAGACTCGGTCTGTTGGGGTCGGGACAGTGCT GTCTGGCAGTTCTGACTTTGACAAGCCATGTGCTGTGAAGACCAAAGAGTCGGGTGTGGGACAGATAAAT ATTCACGACAACTATCTAGTTGGTCTCAAAATGAGGACCATAGCGTGCGGGCCTCCACAGTTGACTGTGG GACTCATAGGCAGCAGGAGGAGCGTGGGTGTTGGGAACGAGCCTGTAGGGGATCTCCTGGAGGATCCTCT TCAGCCTCAGGTTCCATCTGGAATGATGACTGGCTTGGATCACTACATTGAGCGCGTGCAGAAGCTGCTG GCAGAGCAGCAGACGTTGCTGGCTGAGAACTACAGTGAGCTGGCGGAGGCTTTCGGGGAGCCTCATTCAC AGATCGGCTCCCTCAATTCACAACTCATCAGCACCCTGTCGTCCATCAATTCCGTCATGAAGTCTGCAAG CACAGAAGAGCTCAGGAACCCTGACTTCCAGAAAAGCAGTCTGGGTAAAATTGCAGGAAATCACTTGGAA TGTACCTGTAAGTGTGGAGGCCTTCGGTCGGGAGGACTAGTAAATGTACAAACATCCCAGCCTGAGGTGG AGACAGAGACAACAGAAGGCAAGCGGGGCTGTGAGCAGTTCCTCACACAGGGAAATACTCTGCCCCCGGT GAACCTGACAGATGACCAGATAGCCACCGGTCTCTATGTATGTCCAAATAATGAAAACACACTGAAATCC ATCATGAAGAAAAGCGATGGGAATAAAGATTCGAACGGAGCCAAAAAGAATCTTCAGTTCATTGGTGTTA ACGGCGGGTATGAGACAACTTCAAGTGATGATTCCAGCTCGGGTGGAAGCTCTTCTTCCGAGTCAGATGA CGAGTGTGACACCAATGGGTATCCTCCCGAGGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGACCACGAC ACTCGGGGAATGGCGGAAGGGCGCCACGCAGTTAATATTGAAGGTTTCCAGTCTGCCAGGGTGGAAGATG AAGTGCAGGTTCCAGAATGTGAACCTGAGAAGGAGGAAATCAGAGAGAGGTATGAATTAAGTGAAAAGAT GCTGTCTGCCTGCAGCTTATTGAAATATAACATAAATGATCCCAAAGCTTTGGCCAGCAAAGATATGAGG ATCTGTCTGAACACCCTCCAGCATGAGTGGTTCCGAGTGTCCAGTCAGAAGTCCGCTGTGCCAGCCATGG TGGGTGACTACATCGCCGCGTTCGAGGCTGTCTCCCCGGACGTGCTCCGGTACATCATCAACATGGCGGA CGGCAACGGGAACACCGCCCTCCACTACAGCGTGTCCCACTCCAACTTCCAGATCGTCAAGCTGCTTCTG GATGCAGACGTGTGTAACGTAGATCATCAGAACAAGGCAGGGTACACGCCCATCATGCTGGCAGCCCTCG CAGCCGTGGAGGCAGAGAAGGACATGCAGGTCGTAGAAGAACTCTTCAGCTGTGGAGATGTGAATGCCAA GGCCAGTCAGGCAGGACAGACAGCCCTCATGCTGGCCGTCAGTCATGGGCGGATAGACATGGTGAAGGGC CTACTGGCCTGTGGGGCTGACGTCAACATCCAGGATGACGAGGGTTCTACTGCCCTGATGTGTGCCAGCG AACATGGGCATGTGGAGATCGTGAAGCTGCTGCTGGCCCAGCCAGGCTGCAGTGGCCACCTGGAGGACAA TGACGGCAGCACCGCTCTCTCCATAGCTTTGGAAGCAGGACACAAGGACATTGCTGTGCTTCTGTATGCC CACCTCAACTTCTCGAAAGCCCAGTCTCCGAGCACCCCGAGGCTTGGAAGAAAGACCTCTCCTGGTCCCA CTCACCGAGGTTCCTTTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001037197
Insert Size	4083 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001037197.2, NP_001032274.2
RefSeq Size	5170 bp
RefSeq ORF	4083 bp
Locus ID	309429

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