Nos1 (NM_052799) Rat Untagged Clone

CAT#: RN209715

Nos1 (untagged ORF) - Rat nitric oxide synthase 1, neuronal (Nos1), (10 ug)



  "NM_052799" in other vectors (1)

CNY 23,470.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal n-NOS(Ser852) antibody(Phospho-specific)
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms bNOS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN209715 representing NM_052799
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAGAACACGTTTGGGGTTCAGCAGATCCAACCCAATGTAATTTCTGTTCGTCTCTTCAAACGCA
AAGTGGGAGGTCTGGGCTTCCTGGTGAAGGAACGGGTCAGCAAGCCTCCCGTGATCATCTCAGACCTGAT
TCGAGGAGGTGCTGCGGAGCAGAGCGGCCTTATCCAAGCTGGAGACATCATTCTCGCAGTCAACGATCGG
CCCTTGGTAGACCTCAGCTATGACAGTGCCCTGGAGGTTCTCAGGGGCATTGCCTCTGAGACCCACGTGG
TCCTCATTCTGAGGGGCCCTGAGGGCTTCACTACACATCTGGAGACCACCTTCACAGGGGATGGAACCCC
CAAGACCATCCGGGTGACCCAGCCCCTCGGTCCTCCCACCAAAGCCGTCGATCTGTCTCACCAGCCTTCA
GCCAGCAAAGACCAGTCATTAGCAGTAGACAGAGTCACAGGTCTGGGTAATGGCCCTCAGCATGCCCAAG
GCCATGGGCAGGGAGCTGGCTCAGTCTCCCAAGCTAATGGTGTGGCCATTGACCCCACGATGAAAAGCAC
CAAGGCCAACCTCCAGGACATCGGGGAACATGATGAACTGCTCAAAGAGATAGAACCTGTGCTGAGCATC
CTCAACAGTGGGAGCAAAGCCACCAACAGAGGGGGACCAGCCAAAGCAGAGATGAAAGACACAGGAATCC
AGGTGGACAGAGACCTCGATGGCAAATCGCACAAAGCTCCGCCCCTGGGCGGGGACAATGACCGCGTCTT
CAATGACCTGTGGGGGAAGGACAACGTTCCTGTGGTCCTTAACAACCCGTATTCAGAGAAGGAACAGTCC
CCTACCTCGGGGAAACAGTCTCCCACCAAGAACGGCAGCCCTTCCAGGTGCCCCCGTTTCCTCAAGGTCA
AGAACTGGGAGACGGACGTGGTCCTCACCGACACCCTGCACCTGAAGAGCACACTGGAAACGGGGTGCAC
AGAGCACATTTGCATGGGCTCGATCATGCTGCCTTCCCAGCACACGCGGAAGCCAGAAGATGTCCGCACA
AAGGACCAGCTCTTCCCTCTAGCCAAAGAATTTCTCGACCAATACTACTCATCCATTAAGAGATTTGGCT
CCAAGGCCCACATGGACAGGCTGGAGGAGGTGAACAAGGAGATTGAAAGCACCAGCACCTACCAGCTCAA
GGACACCGAGCTCATCTATGGCGCCAAGCATGCCTGGCGGAACGCCTCTCGATGTGTGGGCAGGATCCAG
TGGTCCAAGCTGCAGGTGTTCGATGCCCGAGACTGCACCACAGCCCACGGCATGTTCAACTACATCTGTA
ACCATGTCAAGTATGCCACCAACAAAGGGAATCTCAGGTCGGCCATCACGATATTCCCTCAGAGGACTGA
CGGCAAACATGACTTCCGAGTGTGGAACTCGCAGCTCATCCGCTACGCGGGCTACAAGCAGCCAGATGGC
TCTACCTTGGGGGATCCAGCCAATGTGCAGTTCACGGAGATCTGTATACAGCAGGGCTGGAAAGCCCCAA
GAGGCCGCTTCGACGTGCTGCCTCTCCTGCTTCAGGCCAATGGCAATGACCCTGAGCTCTTCCAGATCCC
CCCAGAGCTGGTGCTGGAAGTGCCCATCAGGCACCCCAAGTTCGACTGGTTTAAGGACCTGGGGCTCAAA
TGGTATGGCCTCCCCGCTGTGTCCAACATGCTGCTGGAGATCGGGGGCCTGGAGTTCAGCGCCTGTCCCT
TCAGCGGCTGGTACATGGGCACAGAGATCGGCGTCCGTGACTACTGTGACAACTCTCGATACAACATCCT
GGAGGAAGTAGCCAAGAAGATGGATTTGGACATGAGGAAGACCTCGTCCCTCTGGAAGGACCAAGCACTG
GTGGAGATCAACATTGCTGTTCTATATAGCTTCCAGAGTGACAAGGTGACCATCGTTGACCACCACTCTG
CCACGGAGTCCTTCATCAAACACATGGAGAATGAATACCGCTGCAGAGGGGGCTGCCCCGCCGACTGGGT
GTGGATTGTGCCTCCCATGTCGGGCAGCATCACCCCTGTCTTCCACCAGGAGATGCTCAACTATAGACTC
ACCCCGTCCTTTGAATACCAGCCTGATCCATGGAACACCCACGTGTGGAAGGGCACCAACGGGACCCCCA
CGAAGCGGCGAGCTATCGGCTTTAAGAAATTGGCAGAGGCCGTCAAGTTCTCAGCCAAGCTAATGGGGCA
GGCCATGGCCAAGAGGGTCAAGGCGACCATTCTCTACGCCACAGAGACAGGCAAATCACAAGCCTATGCC
AAGACCCTGTGTGAGATCTTCAAGCACGCCTTCGATGCCAAGGCAATGTCCATGGAGGAGTATGACATCG
TGCACCTGGAGCACGAAGCCCTGGTCTTGGTGGTCACCAGCACCTTTGGCAATGGAGACCCCCCTGAGAA
CGGGGAGAAATTCGGCTGTGCTTTAATGGAGATGAGGCACCCCAACTCTGTGCAGGAGGAGAGAAAGTAC
CCGGAACCCTTGCGTTTCTTTCCCCGTAAAGGGCCTTCCCTCTCCCATGTTGACTCTGAAGCCCACAGTC
TGGTTGCTGCCCGTGACAGCCAACACAGGAGCTACAAGGTCCGATTCAACAGCGTCTCCTCCTATTCTGA
CTCCCGAAAGTCATCGGGCGACGGACCCGACCTCAGAGACAACTTTGAAAGTACTGGACCCCTGGCCAAT
GTGAGGTTCTCAGTGTTCGGCCTCGGCTCTCGGGCGTACCCCCACTTCTGTGCCTTTGGGCATGCGGTGG
ACACCCTCCTGGAGGAACTGGGAGGGGAGAGGATTCTGAAGATGAGGGAGGGGGATGAGCTTTGCGGACA
GGAAGAAGCTTTCAGGACCTGGGCCAAGAAAGTCTTCAAGGCAGCCTGTGATGTGTTCTGCGTGGGGGAT
GACGTCAACATCGAGAAGGCGAACAACTCCCTCATTAGCAATGACCGAAGCTGGAAGAGGAACAAGTTCC
GCCTCACGTATGTGGCGGAAGCTCCAGATCTGACCCAAGGTCTTTCCAATGTTCACAAAAAACGAGTCTC
GGCTGCTCGACTCCTCAGCCGCCAAAACCTGCAAAGCCCTAAGTCCAGCCGATCGACCATCTTCGTGCGT
CTCCACACCAACGGGAATCAGGAGCTGCAGTACCAGCCAGGGGACCACCTGGGTGTCTTCCCCGGCAACC
ACGAGGACCTCGTGAATGCACTCATTGAACGGCTGGAGGATGCACCGCCTGCCAACCACGTGGTGAAGGT
GGAGATGCTGGAGGAGAGGAACACTGCTCTGGGTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCA
CCCTGCACCATCTTCCAGGCCTTCAAGTACTACCTGGACATCACCACGCCGCCCACGCCCCTGCAGCTGC
AGCAGTTCGCCTCTCTGGCCACTAATGAGAAAGAGAAGCAGCGGTTGCTGGTCCTCAGCAAGGGGCTCCA
GGAATATGAGGAGTGGAAGTGGGGCAAGAACCCCACAATGGTGGAGGTGCTGGAGGAGTTCCCGTCCATC
CAGATGCCGGCTACACTTCTCCTCACTCAGCTGTCGCTGCTGCAGCCTCGCTACTACTCCATCAGCTCCT
CTCCAGACATGTACCCCGACGAGGTGCACCTCACTGTGGCCATCGTCTCCTACCACACCCGAGACGGAGA
AGGACCAGTCCACCACGGGGTGTGCTCCTCCTGGCTCAACAGAATACAGGCTGACGATGTAGTCCCCTGC
TTCGTGAGAGGTGCCCCTAGCTTCCACCTGCCTCGAAACCCCCAGGTGCCTTGCATCCTGGTTGGCCCAG
GCACTGGCATCGCACCCTTCCGAAGCTTCTGGCAACAGCGACAATTTGACATCCAACACAAAGGAATGAA
TCCGTGCCCCATGGTTCTGGTCTTCGGGTGTCGACAATCCAAGATAGATCATATCTACAGAGAGGAGACC
CTGCAGGCCAAGAACAAGGGCGTCTTCAGAGAGCTGTACACTGTCTATTCCCGGGAACCGGACAGGCCAA
AGAAATATGTACAGGACGTGCTGCAGGAACAGCTGGCTGAGTCTGTGTACCGCGCCCTGAAGGAGCAAGG
AGGCCACATTTATGTCTGTGGGGACGTTACCATGGCCGCCGATGTCCTCAAAGCCATCCAGCGCATAATG
ACCCAGCAGGGGAAACTCTCAGAGGAGGACGCTGGTGTATTCATCAGCAGGCTGAGGGATGACAACCGGT
ACCACGAGGACATCTTTGGAGTCACCCTCAGAACGTATGAAGTGACCAACCGCCTTAGATCTGAGTCCAT
CGCCTTCATCGAAGAGAGCAAAAAAGACGCAGATGAGGTTTTCAGCTCCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_052799
Insert Size 4392 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_052799.1, NP_434686.1
RefSeq Size 4592 bp
RefSeq ORF 4392 bp
Locus ID 24598
UniProt ID P29476
Gene Summary enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...