Nos1 (NM_052799) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	bNOS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209715 representing NM_052799 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGAACACGTTTGGGGTTCAGCAGATCCAACCCAATGTAATTTCTGTTCGTCTCTTCAAACGCA AAGTGGGAGGTCTGGGCTTCCTGGTGAAGGAACGGGTCAGCAAGCCTCCCGTGATCATCTCAGACCTGAT TCGAGGAGGTGCTGCGGAGCAGAGCGGCCTTATCCAAGCTGGAGACATCATTCTCGCAGTCAACGATCGG CCCTTGGTAGACCTCAGCTATGACAGTGCCCTGGAGGTTCTCAGGGGCATTGCCTCTGAGACCCACGTGG TCCTCATTCTGAGGGGCCCTGAGGGCTTCACTACACATCTGGAGACCACCTTCACAGGGGATGGAACCCC CAAGACCATCCGGGTGACCCAGCCCCTCGGTCCTCCCACCAAAGCCGTCGATCTGTCTCACCAGCCTTCA GCCAGCAAAGACCAGTCATTAGCAGTAGACAGAGTCACAGGTCTGGGTAATGGCCCTCAGCATGCCCAAG GCCATGGGCAGGGAGCTGGCTCAGTCTCCCAAGCTAATGGTGTGGCCATTGACCCCACGATGAAAAGCAC CAAGGCCAACCTCCAGGACATCGGGGAACATGATGAACTGCTCAAAGAGATAGAACCTGTGCTGAGCATC CTCAACAGTGGGAGCAAAGCCACCAACAGAGGGGGACCAGCCAAAGCAGAGATGAAAGACACAGGAATCC AGGTGGACAGAGACCTCGATGGCAAATCGCACAAAGCTCCGCCCCTGGGCGGGGACAATGACCGCGTCTT CAATGACCTGTGGGGGAAGGACAACGTTCCTGTGGTCCTTAACAACCCGTATTCAGAGAAGGAACAGTCC CCTACCTCGGGGAAACAGTCTCCCACCAAGAACGGCAGCCCTTCCAGGTGCCCCCGTTTCCTCAAGGTCA AGAACTGGGAGACGGACGTGGTCCTCACCGACACCCTGCACCTGAAGAGCACACTGGAAACGGGGTGCAC AGAGCACATTTGCATGGGCTCGATCATGCTGCCTTCCCAGCACACGCGGAAGCCAGAAGATGTCCGCACA AAGGACCAGCTCTTCCCTCTAGCCAAAGAATTTCTCGACCAATACTACTCATCCATTAAGAGATTTGGCT CCAAGGCCCACATGGACAGGCTGGAGGAGGTGAACAAGGAGATTGAAAGCACCAGCACCTACCAGCTCAA GGACACCGAGCTCATCTATGGCGCCAAGCATGCCTGGCGGAACGCCTCTCGATGTGTGGGCAGGATCCAG TGGTCCAAGCTGCAGGTGTTCGATGCCCGAGACTGCACCACAGCCCACGGCATGTTCAACTACATCTGTA ACCATGTCAAGTATGCCACCAACAAAGGGAATCTCAGGTCGGCCATCACGATATTCCCTCAGAGGACTGA CGGCAAACATGACTTCCGAGTGTGGAACTCGCAGCTCATCCGCTACGCGGGCTACAAGCAGCCAGATGGC TCTACCTTGGGGGATCCAGCCAATGTGCAGTTCACGGAGATCTGTATACAGCAGGGCTGGAAAGCCCCAA GAGGCCGCTTCGACGTGCTGCCTCTCCTGCTTCAGGCCAATGGCAATGACCCTGAGCTCTTCCAGATCCC CCCAGAGCTGGTGCTGGAAGTGCCCATCAGGCACCCCAAGTTCGACTGGTTTAAGGACCTGGGGCTCAAA TGGTATGGCCTCCCCGCTGTGTCCAACATGCTGCTGGAGATCGGGGGCCTGGAGTTCAGCGCCTGTCCCT TCAGCGGCTGGTACATGGGCACAGAGATCGGCGTCCGTGACTACTGTGACAACTCTCGATACAACATCCT GGAGGAAGTAGCCAAGAAGATGGATTTGGACATGAGGAAGACCTCGTCCCTCTGGAAGGACCAAGCACTG GTGGAGATCAACATTGCTGTTCTATATAGCTTCCAGAGTGACAAGGTGACCATCGTTGACCACCACTCTG CCACGGAGTCCTTCATCAAACACATGGAGAATGAATACCGCTGCAGAGGGGGCTGCCCCGCCGACTGGGT GTGGATTGTGCCTCCCATGTCGGGCAGCATCACCCCTGTCTTCCACCAGGAGATGCTCAACTATAGACTC ACCCCGTCCTTTGAATACCAGCCTGATCCATGGAACACCCACGTGTGGAAGGGCACCAACGGGACCCCCA CGAAGCGGCGAGCTATCGGCTTTAAGAAATTGGCAGAGGCCGTCAAGTTCTCAGCCAAGCTAATGGGGCA GGCCATGGCCAAGAGGGTCAAGGCGACCATTCTCTACGCCACAGAGACAGGCAAATCACAAGCCTATGCC AAGACCCTGTGTGAGATCTTCAAGCACGCCTTCGATGCCAAGGCAATGTCCATGGAGGAGTATGACATCG TGCACCTGGAGCACGAAGCCCTGGTCTTGGTGGTCACCAGCACCTTTGGCAATGGAGACCCCCCTGAGAA CGGGGAGAAATTCGGCTGTGCTTTAATGGAGATGAGGCACCCCAACTCTGTGCAGGAGGAGAGAAAGTAC CCGGAACCCTTGCGTTTCTTTCCCCGTAAAGGGCCTTCCCTCTCCCATGTTGACTCTGAAGCCCACAGTC TGGTTGCTGCCCGTGACAGCCAACACAGGAGCTACAAGGTCCGATTCAACAGCGTCTCCTCCTATTCTGA CTCCCGAAAGTCATCGGGCGACGGACCCGACCTCAGAGACAACTTTGAAAGTACTGGACCCCTGGCCAAT GTGAGGTTCTCAGTGTTCGGCCTCGGCTCTCGGGCGTACCCCCACTTCTGTGCCTTTGGGCATGCGGTGG ACACCCTCCTGGAGGAACTGGGAGGGGAGAGGATTCTGAAGATGAGGGAGGGGGATGAGCTTTGCGGACA GGAAGAAGCTTTCAGGACCTGGGCCAAGAAAGTCTTCAAGGCAGCCTGTGATGTGTTCTGCGTGGGGGAT GACGTCAACATCGAGAAGGCGAACAACTCCCTCATTAGCAATGACCGAAGCTGGAAGAGGAACAAGTTCC GCCTCACGTATGTGGCGGAAGCTCCAGATCTGACCCAAGGTCTTTCCAATGTTCACAAAAAACGAGTCTC GGCTGCTCGACTCCTCAGCCGCCAAAACCTGCAAAGCCCTAAGTCCAGCCGATCGACCATCTTCGTGCGT CTCCACACCAACGGGAATCAGGAGCTGCAGTACCAGCCAGGGGACCACCTGGGTGTCTTCCCCGGCAACC ACGAGGACCTCGTGAATGCACTCATTGAACGGCTGGAGGATGCACCGCCTGCCAACCACGTGGTGAAGGT GGAGATGCTGGAGGAGAGGAACACTGCTCTGGGTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCA CCCTGCACCATCTTCCAGGCCTTCAAGTACTACCTGGACATCACCACGCCGCCCACGCCCCTGCAGCTGC AGCAGTTCGCCTCTCTGGCCACTAATGAGAAAGAGAAGCAGCGGTTGCTGGTCCTCAGCAAGGGGCTCCA GGAATATGAGGAGTGGAAGTGGGGCAAGAACCCCACAATGGTGGAGGTGCTGGAGGAGTTCCCGTCCATC CAGATGCCGGCTACACTTCTCCTCACTCAGCTGTCGCTGCTGCAGCCTCGCTACTACTCCATCAGCTCCT CTCCAGACATGTACCCCGACGAGGTGCACCTCACTGTGGCCATCGTCTCCTACCACACCCGAGACGGAGA AGGACCAGTCCACCACGGGGTGTGCTCCTCCTGGCTCAACAGAATACAGGCTGACGATGTAGTCCCCTGC TTCGTGAGAGGTGCCCCTAGCTTCCACCTGCCTCGAAACCCCCAGGTGCCTTGCATCCTGGTTGGCCCAG GCACTGGCATCGCACCCTTCCGAAGCTTCTGGCAACAGCGACAATTTGACATCCAACACAAAGGAATGAA TCCGTGCCCCATGGTTCTGGTCTTCGGGTGTCGACAATCCAAGATAGATCATATCTACAGAGAGGAGACC CTGCAGGCCAAGAACAAGGGCGTCTTCAGAGAGCTGTACACTGTCTATTCCCGGGAACCGGACAGGCCAA AGAAATATGTACAGGACGTGCTGCAGGAACAGCTGGCTGAGTCTGTGTACCGCGCCCTGAAGGAGCAAGG AGGCCACATTTATGTCTGTGGGGACGTTACCATGGCCGCCGATGTCCTCAAAGCCATCCAGCGCATAATG ACCCAGCAGGGGAAACTCTCAGAGGAGGACGCTGGTGTATTCATCAGCAGGCTGAGGGATGACAACCGGT ACCACGAGGACATCTTTGGAGTCACCCTCAGAACGTATGAAGTGACCAACCGCCTTAGATCTGAGTCCAT CGCCTTCATCGAAGAGAGCAAAAAAGACGCAGATGAGGTTTTCAGCTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_052799
Insert Size	4392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_052799.1, NP_434686.1
RefSeq Size	4592 bp
RefSeq ORF	4392 bp
Locus ID	24598
UniProt ID	P29476
Gene Summary	enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.