Erbb4 (NM_021687) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209714 representing NM_021687 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGGCGACGGGACTGTGGGTCTGGGGGAGCCTTCTGGTGGCAGCCAGGACCGTCCAGCCCAGCG CTTCTCAGTCAGTGTGTGCCGGAACAGAGAACAAACTGAGCTCTCTCTCTGATCTGGAGCAGCAGTACCG AGCCTTGCGCAAATACTATGAAAACTGCGAGGTAGTCATGGGCAACCTGGAGATCACCAGCATAGAGCAC AACCGGGACCTCTCCTTCCTGCGGTCTATCCGAGAAGTCACAGGCTATGTACTTGTGGCCCTCAACCAGT TTCGTTACCTGCCTCTGGAGAATTTACGCATTATTCGTGGGACAAAACTGTATGAAGATCGCTATGCCTT AGCAATATTCTTAAACTACAGGAAAGATGGCAACTTTGGACTTCAAGAACTGGGATTAAAGAACCTGACC GAAATACTAAATGGTGGAGTCTATGTAGACCAGAACAAATTCCTATGTTATGCTGATACTATACACTGGC AAGATATTGTTCGGAATCCATGGCCTTCCAACATGACTCTGGTGTCAACAATTGGAAGTTCTGGATGCGG AAGATGCCATAAGTCTTGCACTGGTCGATGCTGGGGACCCACAGAAAATCACTGCCAGACCTTGACAAGG ACTGTGTGTGCAGAACAATGTGATGGCAGGTGCTATGGACCCTACGTCAGTGACTGCTGCCATCGAGAAT GTGCCGGAGGCTGCTCAGGACCAAAAGACACTGACTGCTTTGCCTGCATGAACTTCAATGACAGTGGAGC ATGTGTTACTCAGTGTCCCCAAACGTTCGTCTACAATCCAACCACCTTTCAACTGGAACACAACTTCAAT GCAAAGTACACATATGGAGCATTCTGTGTTAAGAAATGTCCACATAACTTCGTGGTAGATTCCAGTTCTT GTGTACGAGCCTGCCCTAGTTCCAAGATGGAAGTCGAAGAAAATGGAATTAAAATGTGTAAGCCTTGCAC TGATATTTGCCCCAAAGCATGTGATGGAATCGGCACCGGATCCTTGATGTCTGCTCAGACTGTGGATTCC AGTAACATTGACAAATTCATAAACTGCACCAAGATCAACGGGAATCTCATCTTTCTTGTCACTGGCATTC ATGGGGACCCTTACAATGCTATTGACGCCATAGACCCAGAGAAACTGAATGTCTTTCGGACAGTCAGAGA AATAACAGGTTTCCTGAACATACAGACTTGGCCCCCAAATATGACAGATTTCAGTGTTTTCTCCAACCTT GTAACCATTGGAGGAAGAGTCCTCTACAGTGGTCTGTCATTGCTTATCCTCAAACAACAAGGTATCACTT CACTACAGTTCCAGTCTCTGAAGGAAATCAGTGCGGGCAATATCTACATCACGGACAACAGCAACCTGTG TTATTACCACACCATCAACTGGACAACACTGTTCAGCACCGTTAACCAGAGGATAGTGATCCGAGACAAC AGGAGGGCTGAGAACTGTACTGCTGAAGGGATGGTGTGTAACCACCTGTGTTCAAATGATGGTTGTTGGG GACCTGGGCCAGACCAGTGTCTGTCATGTCGGCGCTTCAGCAGGGGAAAGATCTGTATAGAGTCCTGCAA CCTTTATGATGGGGAGTTTCGAGAGTTTGAAAATGGCTCCATCTGTGTTGAGTGTGACTCCCAGTGTGAG AAAATGGAAGACGGACTCCTCACATGCCATGGACCGGGACCTGACAACTGTACAAAGTGTTCTCATTTTA AAGATGGTCCAAACTGTGTAGAGAAATGTCCAGATGTCCTACAGGGAGCAAACAGTTTCATATTTAAGTA CGCAGATCAGGATCGGGAGTGCCACCCTTGCCATCCAAACTGCACCCAGGGGTGTAACGGTCCCACTAGT CATGACTGCATTTACTACCCATGGACGGGCCATTCCACTTTACCACAACATGCTAGAACTCCACTGATTG CAGCCGGAGTCATTGGTGGGCTCTTCATCCTGGTCATCATGGCTCTGACATTTGCCGTTTATGTCAGAAG GAAGAGCATCAAAAAGAAACGCGCTTTGAGAAGATTCCTGGAGACCGAGTTAGTCGAGCCCTTAACCCCT AGTGGCACAGCACCCAATCAAGCTCAACTTCGAATTTTGAAGGAAACAGAGCTAAAGAGGGTAAAAGTCC TTGGCTCGGGAGCATTTGGAACCGTTTATAAAGGAATCTGGGTACCTGAAGGAGAAACTGTGAAAATCCC TGTGGCTATTAAGATCCTCAATGAGACAACTGGCCCCAAAGCCAATGTGGAGTTCATGGATGAGGCACTG ATTATGGCAAGTGTGGATCACCCACACCTAGTGCGTTTACTGGGTGTGTGTTTGAGCCCCACTATCCAGT TGGTTACTCAGTTGATGCCACATGGCTGCCTACTGGAATATGTCCACGAACACAAGGATAACATCGGATC ACAACTGCTGTTGAACTGGTGTGTCCAGATTGCTAAGGGAATGATGTATCTGGAGGAAAGGCGGCTTGTT CATCGGGATCTGGCAGCCCGCAATGTGTTGGTGAAATCTCCAAATCATGTTAAAATCACAGACTTTGGAC TGGCCCGGCTCTTGGAAGGAGATGAAAAAGAATACAATGCTGACGGTGGCAAGATGCCAATTAAATGGAT GGCTCTGGAATGTATACATTATAGGAAATTCACACATCAAAGCGATGTTTGGAGCTACGGTGTCACTATA TGGGAACTGATGACCTTTGGAGGAAAGCCCTATGATGGAATTCCAACGCGAGAAATCCCTGATTTATTAG AGAAGGGAGAGCGTTTGCCTCAACCTCCCATCTGCACTATTGACGTTTACATCGTCATGGTCAAATGTTG GATGATCGATGCTGACAGCAGACCTAAATTCAAAGAACTGGCTGCTGAGTTTTCAAGGATGGCTAGAGAC CCTCAAAGATACCTAGTAATTCAGGGGGATGATCGCATGAAGCTTCCCAGTCCAAACGACAGCAAATTCT TCCAGAATCTCTTGGATGAAGAGGATTTGGAAGATATGATGGACGCTGAGGAATATTTGGTCCCCCAGGC TTTCAATATCCCACCTCCTATCTACACATCCAGAACAAGAATTGACTCCAATAGGAGTGAAATTGGACAC AGCCCTCCTCCTGCCTACACCCCCATGTCGGGAAGTCAGTTTGTGTACCAGGATGGGGGTTTCGCTACAC AACAAGGAATGCCCATGCCCTACACAGCCACAACCAGCACCATACCAGAGGCTCCAGTCGCCCAGGGTGC AACGGCTGAGATGTTTGATGACTCCTGCTGTAATGGTACCCTGCGAAAGCCAGTGGTACCCCACGTCCAA GAGGACAGTAGCACTCAGAGGTATAGTGCCGACCCCACAGTGTTCGCCCCAGAACGGAACCCACGAGCAG AACTGGATGAAGAAGGCTACATGACTCCCATGCATGACAAGCCAAAACAAGAATATCTGAATCCTGTGGA AGAGAACCCTTTTGTGTCCCGGAGGAAGAATGGAGACCTTCAAGCTTTAGATAATCCAGAGTATCACAGC GCTTCCAGCGGTCCCCCCAAGGCAGAGGATGAGTACGTGAATGAGCCCCTTTATCTCAACACCTTCACCA ACGCCTTGGGAAATGCAGAGTACATGAAAAACAGCTTACTGTCTGTGCCAGAGAAAGCCAAGAAAGCATT TGACAACCCCGACTACTGGAACCACAGCCTGCCACCCCGGAGCACTCTTCAGCACCCAGACTACCTGCAG GAATACAGCACAAAATATTTTTATAAACAGAATGGACGGATCCGCCCTATTGTGGCAGAGAATCCTGAGT ACCTCTCAGAGTTCTCGCTGAAGCCAGGCACTATGCTGCCCCCTCCGCCCTACAGACACCGGAATACTGT GGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_021687
Insert Size	3927 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021687.1, NP_067719.1
RefSeq Size	4060 bp
RefSeq ORF	3927 bp
Locus ID	59323
UniProt ID	Q62956
Gene Summary	binds neuregulins; involved in epithelial-mesenchymal interactions during development; may play a role in tooth morphogenesis [RGD, Feb 2006]

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