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Dclk2 (NM_001009691) Rat Untagged Clone
CAT＃: RN209497
Dclk2 (untagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug)
CNY 9,880.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | CL2; CLICK-II; CLICK2; Dck2; RGD1308384 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN209497 representing NM_001009691
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCACCAGGAGCATTGAGCTGGAACATTTTGAAGAACGGGACAAAAGGCCGCGGCCAGGGTCGC GGAGAGGAGCTCCCAGCTCCTCCGGGGGCAGCAGCATCTCTGGCCCCAAGGGCAACGGGCTCATCCCCAG CCCGGCGCACAGTGCTCACTGCAGCTTCTACCGCACGCGGACCTTGCAGGCCCTCAGCTCGGAGAAGAAG GCCAAGAAGGCGCGGTTCTACCGGAATGGGGACCGCTACTTCAAAGGCCTGGTCTTTGCGATCTCCAGCG ACCGTTTCCGTTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCGCTGTCTGACAATGTGAACCTGCC CCAGGGCGTCCGCACAATCTACACCGTAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA GGTGAAAGTTACGTGTGTGCCTCCAACGAACCATTCCGTAAAGTTGACTACACCAAAAACGTTAACCCGA ACTGGTCTGTGAACATCAAGGGTGGTACTACCCGCACCTTGGCCGTGGCCTCGGCCAAGAGTGAGGTGAA AGAAAGCAAGGACTTCATCAAACCCAAGCTCGTGACTGTGATTCGAAGTGGGGTGAAGCCTAGAAAGGCC GTGCGCATCCTTCTGAATAAAAAGACTGCCCATTCCTTTGAGCAGGTCCTAACGGATATCACCGAAGCCA TTAAACTAGACTCCGGCGTGGTGAAGAGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGA CTTTTTTGGAGACGATGATGTTTTTATTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTT GTCCTGGATCATAGTGAGTGCCGTGTCCTGAAGTCATCTTATTCTCGGGCCTCAGCTGCTAAGTATTCTG GATCCAGAAGCCCAGGGCTCTCCCGCCGCAGCAAGTCACCAGCTTCAGTAAAGAGGGCTGGCCACTCCAG TGCCTATTCTACAGCCAAATCCCCAGTGAATGGAACTCCCAGCAGCCAGCTTTCCACTCCTAAGTCCACC AAGTCCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGACTGAAGCAGATTTCTGCTCAGGGCA GATCTTCTTCCAATGTAAACGGTGGGCCCGAACTTGACCGTTGCATGAGTCCTGAAGGCGTGAATGGAAA CAGGTGCTCCGAGTCATTCACTCTTCTGGAGAAATACAGAATCGGGAAGGTCATCGGAGACGGCAACTTT GCCGTAGTTAAAGAATGTATGGACAGGTCCACTGGAAAGGAGTTTGCATTAAAGATTATTGACAAAGCCA AATGCTGTGGAAAGGAACATCTGATTGAGAACGAAGTGTCAATCCTGCGCCGGGTGAAGCACCCCAACAT CATCATGCTGGTTGAGGAGATGGAAACAACAACTGAGCTCTTCCTAGTGATGGAACTGGTCAAAGGTGGA GATCTCTTCGACGCGATCACTTCTTCAACCAAGTACACCGAGAGAGATGGCAGTGCCATGGTGTACAACC TAGCCAGCGCCCTCAGATACCTGCACGGCCTCAGCATCGTGCACAGAGATATCAAACCTGAGAATCTGCT GGTGTGCGAATACCCAGACGGAACCAAGTCGCTGAAGCTGGGAGACTTTGGGCTGGCGACGGTGGTTGAA GGCCCGTTGTACACAGTCTGTGGCACCCCAACTTACGTGGCACCCGAGATCATTGCTGAAACAGGTTACG GCCTGAAGGTGGATGTTTGGGCAGCTGGTGTGATTACGTACATTCTTCTCTGTGGATTCCCACCGTTCCG GAGTGAGAACAACCTCCAGGAGGATCTCTTTGACCAGATCTTGGCCGGAAAGCTGGAGTTTCCAGCTCCC TACTGGGACAACATTACAGACTCTGCCAAGGAGTTAATCAGTCAAATGCTTCAGGTAAACGTTGAAGCTC GCTGTACTGCGGGAGAAATTCTGAGTCACCCCTGGGTGTCAGATGATGCATCCCAGGAGAATAATATGCA AGCCGAGGTTACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACCACCACT GGGGTCTCCGTTATCATGAACACGGCTCTAGATAAAGAGGGGCAGGTTTTCTGCAGCAAGCACTGTCGGG ACAGCAGCAAATCATCCAGGGAGCAGACCTCAGCCCGGGAGGCCCCGCCCCCACCGGAGTCTCCCAGGCC CCCTGGTCCTCCAGCCACCTCTGGCTGTGATCCTGCCGGGACCTGGCGTCGCCATCGAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001009691 |
| Insert Size | 2304 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001009691.3, NP_001009691.3 |
| RefSeq Size | 3969 bp |
| RefSeq ORF | 2304 bp |
| Locus ID | 310698 |
| UniProt ID | Q5MPA9 |
| Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that this gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010] Transcript Variant: This variant (2) is an exon-5-containing transcript (PMIDs:15611072 and 18075264). It encodes the longer isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR209497 | Dclk2 (Myc-DDK-tagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug) |
CNY 9,880.00 |
|
| RR209497L3 | Lenti ORF clone of Dclk2 (Myc-DDK-tagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug) |
CNY 11,690.00 |
|
| RR209497L4 | Lenti ORF clone of Dclk2 (mGFP-tagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug) |
CNY 12,830.00 |
