Prtg (NM_001037651) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1307157
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209418 representing NM_001037651 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTCCCGTGCGCCCGGGGATGCTGCCCCTGCTGCTGTTGCTGCTACTGCCGCCGCTCGGCTCGG TTCCAGGAGTGTGGAGCTTTAGCGAACTGTTCTTCATGAAAGAACCACAGGACGCAACGGTCACAAGGAA AGACCCAGTCTTCTTAGATTGCCAGGCCCATGGAGAAGGTCCCATTAAGGTCACCTGGTTGAAAAATGGA GCAAAATTGTCTGAAAATAAACGGATCCAGGTTCTATCTAACGGCTCTTTATACATCAGTGAGGCGGAAG GCAGGCGGGGAGAGCAATCGGATGAAGGATTTTATCAGTGCTTGGCTGTAAACAAATATGGGGCCATTCT TAGTCAGAAAGCTCATCTGACATTGTCAACCATCTCTGCGTTCGAGGTCCATCCAGTGTCTACCGAAGTC CCCGAAGGGGGAGTTGCTAGATTTTCATGCAAGATTTCATCAACGCCCCCTGCGGTCATAACATGGGAAT TTAATAGGACAGCCCTGCCTATGACCATGGACAGCAGGGTGACTGCCCTGCCCTCAGGAGTGCTGCAGAT CTATGACGCTGGCCCGGAGGATGCTGGGAAATACCGCTGTGTTGCAGCCACGCATGCTCATAAGCGTAAA AGCATGGAGGCCTCGCTAACCATAGTTCCAGCTAATGAGACCAGATCCTTCTACATGCCCACCATTATAG CCAGTCCACAGAATGTAACCGCGTCTCTACATCAGACAGTTGTGCTAGAGTGTATGGCTACAGGATATCC CAAGCCCATCATTTCCTGGAGCCGTCTTGATCACAAGTCCATTGATGTCTTCAATACTCGGGTACTCGGA AATGGCAATCTCATCATATCAGATGTCAAGCTTCAGCATGCTGGAGTGTATGTTTGTCGGGCCACCACCC CAGGCACTCGCAACTTCACAGTTGCTATGGCAACGTTAACTGTATTAGCTCCTCCTTCATTTGTGGAATG GCCTGAAAGTTTAACAAGGCCACGAGCTGGCACTGCACGGTTTGTGTGTCAAGCAGAAGGAATTCCCTCA CCCAAAATGTCCTGGTTGAAAAATGGGAGAAGGATACATTCAAATGGCAGGATTAAAATGTACAACAGCA AATTGGTCATTAACCAGATTATCCCAGAAGATGATGCTATCTATCAGTGCATGGCTGAAAACAGCCAAGG ATCTGTTCTGTCTCGAGCCAGACTGACTGTGGTGATGTCGGAAGATAGACCCAGCGCTCCTTACAACGTT CATGCCGAAACCATGTCGAGCTCTGCTATCCTTCTAGCATGGGAGCGACCACTGTACAATTCAGACAAGG TCATTGCCTACTCTGTACACTACATGAAGGCTGAAGGTCTAAATAACGAGGAATATCAGGTGGTCCTTGG AAATGACACAACTCATTATATCATCGATGACTTGGAACCAGACAGCAACTATACTTTCTACATCGTGGCG TACATGCCCATGGGAGCCAGCCAGATGTCGGATCATGTGACACAGAACACGCTAGAAGACGTGCCCCTAA GGCCTCCCGAAATTAGCTTGACGAGTCGAAGTCCCACCGACATTCTCATCTCCTGGCTGCCCATCCCGGC CAAGTACCGACGGGGCCAAGTGGTGCTGTACCGGCTGTCCTTCCGCCTGAGCACTGAGAATTCCATCCAA GTTGTAGAACTCCCGGGGACTGTGCACGAGTACCTTCTGGAAGGCCTGGAGCCAGACAGTGTCTATCTGG TCCGGATTACTGCTGCTACCCGAGTGGGGCTGGGAGAGTCATCGGTGTGGACGTCACACAGGACGCCCAA AGCCACGAGCGTGAAAGCCCCTAAGTCTCCTGAACTGCATTTGGAGCCACTGAATTGTACTACCATCTCT GTAAGGTGGCTTCAAGACACCGAGGACCCGGCTGCCATTCGGGGCTACAAGCTGTTTTATAAAGAAGAAG GGCAGCAGGAACATGGACCGATCTTCCTGGACACCGGTGACCTGCTGTATACGCTCAGTGGCTTGGACCC CAGAAGAAAATACCATGTGAGGCTACTGGCTTACAACAATCTGGAAGATGGCTACCAGGCCGACCAGACC GTCAGTACTCCCGGATGCGTGTCTGTCCGTGACCGCATGGTCCCTCCGCCACCGCCACCCCACCATCTGT ACGCGAAGGCTAATACCTCCTCTTCTATCTTCCTGCACTGGAGGAGGCCTGCGTTCACCACCGCCCAAGT CATTAACTACACCATCCGCTGTAACCCCGTTGGCCTGCAGAACGCCTCTCTGGTTCTCTACCTGCAAACA TCAGAAACCCACATGCTGGTTCAAGGACTAGAGCCAAACACCAAGTATGAGTTCGCTGTCCGACTGCACG TGGATCAGCTCTCCAGTCCCTGGAGTCCCGTGGTCTACCACACCACACTGCCGGAAGCACCCACAGGCCC CCCAGTGGGGGTGAAGGTGACGCTGATAGAAGATGACACTGCCCTTGTCTCTTGGAAACCCCCAGATGGC CCAGAGACAGTGGTGACGCGCTACACCATCTTGTATGCGTCCAGGAAGGCCTGGATCGCCGGCGAGTGGC AGGTCCTACATCGAGAAGGGGCAATAACCATGGCTCTGCTGGAAAACCTGGTGGCAGGAAACGTGTACAT TGTCAAGATCTCAGCCTCCAACGAGGTGGGAGAAGGGCCCTTCCCAAATTCCGTGGAGCTGGCCGTCCTC CCCAAGGACGCTTCAGAATCGAACCAGAGGCCTAAGCGTTTGGATTCTTCTGATGCCAAAGTTTATTCAG GCTATTACCACCTGGACCAAAAGTCAATGACCGGCATTGCAGTAGGCGTTGGCATAGCCTTGACCTGTAT CCTCATCTGTGTTCTCATCTTGATATACCGAAGCAAAGCCAGGAAGTCATCCGCCTCTAAGACAACGCAG AGTGGAACCCAGCCGTTATCCCGAGCCAGTGCCTCTGTAGCAGCGGGGAGTGACATGGGGAAGAACCTGG AGAGAGCTACAGAAAACGAAGAGTCCTCAGTACCCATGATGCCAAGCTGCTTCATTGATGCGAAGGGAGG GACTGACCTGATCATCAATAGCTATGGTCCTATTATTAAAAACAACCCTAAGAAAAAGTGGCGTTTTTTC CAAGACACTAAGAAGATCAAAGTTGAACAGACTCAAAGAAGAATTACTCAGACCGTGTGCTTTTACCAGC CAGGCACCACAGTGCTGATCAGCGATGAAGACTCCCCTAGCTCCCCAGGCCAGACCACCAGCTTCCCAAG ACCTTTTGGGCCCACCACCCTGGACACTGAGCACTCAGCAAACAGTGAAGGCAGCCACGAGACAGGGGAT TCTGGAAGGTTTTCCCATGAGTCCAATGATGAGATACATCTGTCCTCTGTCATCAGCAGCACACCCCCCA CCTCGAATTCCCTCACCTGTGGTGATTCTGATGGGGATGCTGCACCGAAGAAGCATGGAGACCCTGCCCA GCCCTTGCCAGCTGAGCAGACCTCAGCCCCACAGCCAACACCTGCTGGGCTGCGACATGCTGCCGAGAGT GTTCCCGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001037651
Insert Size	3582 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001037651.1, NP_001032740.1
RefSeq Size	5326 bp
RefSeq ORF	3582 bp
Locus ID	315806
UniProt ID	Q2VWP9
Gene Summary	May play a role in anteroposterior axis elongation.[UniProtKB/Swiss-Prot Function]

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