Dysf (NM_001107869) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209414 representing NM_001107869 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTGCTGCCTGCTGGCAAGGGCCAGCAACCTCCCCAATGTGAAGAAGGACCGGCGCAGTGACCCTG TTGCCAGCCTGATTTTTCGAGAAGCCAAGATCCCACTCCAGGAGGTCCTTGCCACCCCCAGCCTATCTGC CAGCTTCAACGCACCTCTGCTGGACTCCAAGCAGCAACCCACAGGGGCCTCTCTGGTCCTGCAGGTATCC TACACACCGCCCCCAGGAGCTGCGCCCTTGTTCCCACCACCCGCTTCCCTGGCACCGTCCCCAACACTGC CTGACATGGACCTGGTGGCTGACACAGGAGGAGAGGAAGACACGGAAGACCAGGGCCTGACTGGAGATGA AGCTGAGCTCTTCCCAGATCCGAGTGTTGCTGTGGGCCCAGGGGGACCAACGACGCCCAAGAAGCCACCT TCCCATCCTCCTCCCCACTATCCAGGGGGGAAAAGGAAAAGAAGCTCTGCTCCACCTAGAAAGCTGCTGT CAGACAAGCCACAGGACTTCCAGATCAGAGTCCAGGTGATTGAGGGGCGTCAGCTGCCTGGGGTGAACAT CAAGCCTGTGGTCAAGGTCACAGCTGCTGGGCAGACCAAGCGGACTCGAATCCAGAAGGGAAATAGCCCA CTTTTCAATGAGACTCTTTTCTTCAACGTATTTGACTCTCCCTTGGAACTGTTTGATGAGCCCATCTTCA TCACGGTGGTAGACTCACGCTCCCTCAGGACAGATGCCCTCCTTGGCGAGTTCCGGATGGATGTGGGCAC CGTGTACAGAGAGCCCCGACATGCTTATCTCCGGAAGTGGCTGCTGCTCTCCGATCCCGATGACTTCTCT GCAGGGGCCAGAGGCTACCTCAAAGCCAGCCTGTGTGTGCTGGGGCCTGGGGACGAAGCTCCCCTAGACA AGAAAGACCCTTCTGAAGACAAAGAGGACATTGAAGGCAACCTGCTTAGGCCCACTGGTGTGGCCCTTCG TGGTGCCCATTTTTGCCTGAAGCTCTTCCGGGCTGAGGACTTACCACAGATGGACGATGCTGTGATGGAC AACGTCAAGCAAATCTTTGGCTTTGATAGCAACAAGAAGAACCTGGTGGACCCCTTTGTGGAAGTCAGCT TTGCTGGGAAAATGCTTTGCAGCAAGATCCTGGAGAAGACTGCTAACCCTCAGTGGAACCAGAACATCAC ATTGCCTGCGATGTTTCCCTCTATGTGTGAAAAAATGAGGATTCGTGTCATGGACTGGGACCGCCTCACT CACAATGACACTGTGGCTACCACCTACTTGGGTATGTCGAAAATCTCTGCCACTGGAGGAGAAATAGAAG AGGAGCCTGCAGGTGTACTCAAGAGTCCAGCCACTGACCTGGATGACAACCTGGGCTTCCTCCCCACTTT TGGGCCCTGCTATGTGAACCTCTATGGCAGTCCCCGAGAGTTCACGGGCTTCCCAGATCCTTATGCGGAG CTCAACACAGGAAAGGGAGAAGGTGTGGCATATAGGGGCAGGCTTCTGTTGTCCCTGGAGACCAAACTGG TGGAGCACAGTGAACAGAAGGTGGAAGACCTTCCTGCTGATGACATCTTGAGAGTGGAGAAGTACCTGCG GAGGCGCAAATATTCCCTCTTTGCTGCCTTCTACTCAGCCACAATGCTGCAGGATGTGGATGATGCCATC CAGTTCGAGGTCAGCATTGGGAACTACGGAAACAAGTTTGACACCACCTGCTTGCCGTTGGCCTCTACCA CCCAATACAGCCGGGCAGTCTTTGATGGATGCCACTACTATTATTTGCCCTGGGGCAACGTGAAGCCTGT GGTAGTACTGTCCTCATACTGGGAAGACATCAGCCATCGAGTCGAGACTCAGAACCAGCTCCTCAGGATC GCTGACCGACTGGAAGCTAACCTGGAGCAAGTCCACCTGGCTCTGAAGGCACAGTGTTCCTCTGAGGATG TGGACGCCCTGGTGGCTCAGTTGACAGATGAGCTCCTTGCAGACTGCAGCCAGCCCCTGTGTGACATCCA TGAAATACCTTCTGCCACCCACCTTGATCAGTACCTGTTCCGCCTCCGCTCCCGTCACCTGAGCCAGATC AAAGAAGCTGCCCTGGCCTTGAAGCTTGGCCACAGTGAGCTCTCCACTGCCCTGGAGCAGGCCGAGGACT GGCTTCTGCATCTCCGTGCCCTGGCTGAGGAGCCCCAGAACAGCCTGCCAGATATCATCATCTGGATGCT GCAGGGTGACAAGCGTGTGGCCTACCAACGGGTGCCTGCCCATGAAGTCCTCTTCTCCCGGCGGGGCCCC AGCTACTGCGGCAGGAACTGTGGGAATCTGCAGACCATCTTTCTGAAATATCCTATGGAAGGCATGCCTG GGCCCCGGATGCCGGTGCAGATCCGGATCAAGCTCTGGTTTGGGCTCTCAGTGGATGAGAAGGAATTCAA TCAGTTTGCAGAGGGGAAGCTCTCTGTCTTTGCTGAAACGTATGAGAACCAGACAAAGCTGGCGCTGGTC GGGAACTGGGGCACGACGGGCCTCACCTACCCCAAGTTTTCTGACGTCACAGGCAAGATCAAACTACCGA AGGATAGCTTCCGCCCCTCAGCTGGCTGGGCCTGGGCAGGAGACTGGTTCGTGTGTCCAGAGAAGACCCT GCTCCATGATGCTGACGCCGGTCATCTGAGCTTTGTGGAGGAGGTGTTTGAGAACCAGACTCGGCTACCT GGAGGCCAGTGGATCTATATGAGTGACAACTACACCGACGTGAATGGGGAGAAGGTGCTTCCAAAGGACG ATATTGAATGCCCGCTGGGTTGGAAGTGGGAAGATGAAGAATGGTCCACAGACCTCAATCGGGCTGTTGA TGAACAAGGCTGGGAGTACAGCATCACCATACCCCCAGACCGAAAGCCCAAACACTGGGTCCCTGTTGAG AAGATGTATTACACTCACAGGCGTCGACGCTGGGTCCGACTGCGCAGGAGGGACCTCAGCCAGATGGAGG CACTGAAGAAGCACAGGCAGGCAGAGGCTGAGGGCGAGGGCTGGGAATACGCCTCTCTCTTTGGCTGGAA GTTCCACCTGGAGTACCGCAAGACAGATGCCTTTCGCCGCCGCCGCTGGCGCCGCCGCATGGAGCCACTG GAGAAGACGGGGCCTGCAGCGGTGTTTGCCCTTGAGGGGGCCCTGGGCGGCATGGTGGATGACAAGAGCG AAGATTCCATGTCTGTGTCTACCCTGAGCTTTGGCGTTAACAGACCCACAATCTCCTGCATCTTTGACTA TGGGAACCGCTACCATCTGCGTTGCTACCTGTACCAGGCCCGGGACCTGCCCGCCATGGACAAAGACTCC TTCTCTGATCCCTATGCCATTGTCTCCTTCCTGCATCAAAGCCAGAAGACGGTGGTGGAGAAGAACACCC TGAACCCCACCTGGGATCAAACCCTCATCTTCTATGAGATAGAGATCTTTGGCGAGCCGGCCAGTGTCGC CGAGCGGCCACCCAGCATTGTGGTGGAGCTGTATGACCATGACACCTATGGCGCTGACGAGTTCATGGGC CGCTGTATTTGTCAGCCAAGCCTGGAACGGATGCCCCGCCTGGCCTGGTTCCCACTGACCAGGGGCAGCC AGCCTGCCGGAGAGCTGCTGGCAGCGTTCGAGCTTATCCAGAGAGAGAAGCCAGCCATCCATCACATCCC TGGCTTTGAGATGCACGAAACTTCAAGGATTTTGGATGAGACGGAGGACACAGACTTGCCTTACCCACCA CCTCAAAGGGAGGCCAACATCTACATGGTTCCCCAGAACATCAAGCCAGCTCTCCAGCGTACAGCCATCG AGATCCTGGCCTGGGGCCTTCGGAACATAAAGAGCTATCAGATGGCCAGCGTGTCTTCCCCCAGCCTCGT GGTGGAGTGTGGTGGGCAGACAGTGCAGTCCTGTGTCATCAGAAACCTTCGGAAGAACCCCAACTTTGAC GTCTGTACTCTGTTCATGGAAGTGATGTTACCCAGAGAGGACCTCTACTGTCCCCCTATTGTGGTCAAGG TCATTGATAATCGCCAGTTTGGCCGCAGGCCAGTTGTGGGTCAGTGCACCATTCGCTCCCTGGAGAACTT CCTGTGTGACCCGTACTCAGCAGAGAGTCCATCCCCGCAAGGTGGCCCAGATGACGTGAGCTTACTCAGC CCTGGGGAAGACGTGCTCATTGACATTGATGACAAGGAGCCTCTCATCCCTGTCCAGGAGGAGGAGTTCA TTGACTGGTGGAGCAAATTCTTTGCTTCTATCGGGGAGAGAGAAAAGTGTGGTTCCTACCTGGAGAAGGA TTTTGACACCCTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCCTTTGGAGGCCTGTCCGACTTT TGTAATACCTTCAAGCTCTACCGGGGCAAGACTCAGGAGGACACAGAAGATCCATCTGTCATCGGAGAAT TTAAGGGTCTCTTCAAAATTTATCCCCTCCCAGAAGATCCAGCCATCCCTATGCCCCCGAGACAATTCCA TCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGGATTTACATCATCCGAGCATTTGGCTTACAG CCCAAGGACCCCAATGGCAAGTGCGACCCTTACATCAAGATCTCTATAGGGAAGAAATCAGTGAGTGACC AGGATAACTACATCCCTTGTACCCTGGAGCCTGTATTTGGAAAGATGTTTGAACTCACCTGCACTCTACC TCTGGAGAAGGACTTGAAGATCACACTCTATGACTATGACCTGCTCTCCAAGGATGAGAAGATTGGGGAG ACAGTCATTGATCTGGAGAATAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGCCTTCCACAGACCTACT GTGTCTCTGGACCAAATCAGTGGAGAGATCAACTCCGCCCTTCCCAGCTCCTCCACCTCTTCTGCCAGCA GCACAGGGTCAAGGCCCCCGTGTACCGGACAGACCGAGTGACGTTTCAGGATAAGGAATACACCATTCAG GAGATAGAGGCTGGCAGACTCCCAAACCCACACCTGGGCCCGGTGGAGGAACGCTTAGCCCTGCATGTCC TTCAACAACAAGGCTTGGTCCCTGAGCATGTGGAGTCTCGGCCTCTGTACAGTCCTCTGCAGCCAGATAT TGAGCAGGGGAAGCTACAGATGTGGATTGACATATTTCCAAAGGTGCTGGGCCGGCCTGGACCTCCCTTC AACATCACCCCACGGAAAGCTCGAAGGTTTTTTCTGCGTTGTATTATTTGGAACACAAAAGATGTGATCT TGGATGACCTCAGCCTCACGGGGGAGAAGATGAGTGACATCTATGTGAAAGGCTGGATGGTGGGATTCGA AGAACACAAACAGAAGACAGATGTGCACTACCGTTCCCTGGGCGGGGAGGGCAATTTCAACTGGAGGTTC GTGTTTCCCTTCGACTATCTGCCCGCTGAGCAAGTCTGTGCTGTTGCCAAGAAGGATGCTTTCTGGAGAC TCGACAAGACAGAGAGCAAGATCCCAGCACGTGTGGTCTTCCAGATTTGGGACAATGACAAGTTCTCCTT TGATGACTTTCTGGGCTCCCTGCAGCTGGATCTCAACCGAATGCCCAAGCCAGCCAAGACGGCTGAGAAG TGCTCCTTGGACCAGCTGGATGACACCTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGAAGACAG TGAAAGGATGGTGGCCTTGTGTGACAGAGGAGGGCGCGAAGAAGATCCTGGCGGGGAAGCTGGAAATGAC CTTGGAGATCGTTGCAGAAAGTGAACATGAGGAGCGGCCTGCTGGCCAGGGTCGGGATGAACCCAACATG AATCCAAAGCTAGAAGATCCCAGGCGCCCTGACACCTCTTTCCTGTGGTTCACCTCACCATACAAGACCA TGAAGTTCATCTTGTGGCGACGATTCCGGTGTGCCATCATCCTCTTCATCATCCTCTTCATCCTCCTGCT CTTCCTGGGTGTCTTTGTCTACGCCTTCCCGAACTATGCTGCCATGAAGCTGGTGAAGCCCTTTAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107869
Insert Size	6090 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107869.1, NP_001101339.1
RefSeq Size	6319 bp
RefSeq ORF	6090 bp
Locus ID	312492

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