United States
Germany
Japan
United Kingdom
China
Sprtn (NM_001106199) Rat Untagged Clone
CAT＃: RN209341
Sprtn (untagged ORF) - Rat similar to hypothetical protein (RGD1559496), (10 ug)
CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1559496 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN209341 representing NM_001106199
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGGATCTGGTGGTCGCGCTGCGGCTGCAGGAGGAGTGGGACGTGCAGGCGGCGCGGCGCGCGG CGGCGCGGGAGCCCCTATCGTTGGTGGACGCGTCCTGGGAGCTGGTGGACCCCACCCCGGACCTGCAGGC GCTCTTCCTGCAGTTTAACGATCGCTTCTTCTGGGGTCAGCTGGAGGCCGTGGAGGTGAAGTGGAGTGTG CGCATGACACTGTGTGCTGGCATCTGCACCTATGAAGGGAGAGGAGGCATGTGCTCCATCCGTCTCAGTG AACCCTTGTTAAAACTCAGACCAAGAAAGGACCTTGTAGAGGTCTACCACACTTTCCACGATGAGGTGGA TGAGTATCGCCGGCACTGGTGGCGCTGCAATGGACCCTGCCAGCACAAACAGCCCTACTATGGCTATGTG AAGCGTGCCACCAACAGGGCTCCCTCTGCTCATGACTACTGGTGGGCTGACCACCAGAAAACTTGTGGAG GCACTTACATAAAAATCAAGGAGCCAGAGAACTATGCAAAGAAAGGCAGAGGAAAGACAAAAGCAGGCAA GCAGCCCACATCGGCAGTGGAGAATAAAGATAAACTGTGCAGAGGAGAGGCCCAGCCGCTCATCCCATTC AGTGGCAAAGGGTATGTTCTTGGAGACACAAGCACTTGCCCTTCAGCTGGGAAGCTGAACACCTCACACA TGGTTAATGACACCAAAGGTCTCTCAGGTCAAGACCATTCAGCGAGTGGCCTGAAGCTCGATTCCAACGT TGAGGTGAAATGTGAACAGAACGGTCTTCCAAATAAGAAGCCTCATCTTGTCACCCCTCTTCCCACTGCT AGTCACCAAAGTGTCCTGAGCAGCTACTTCCCCAGAGTCTCTGTTGCCAACCAAAAGGCTTTCAGAAATG TAAATGGATCCCCAGTGAAGAGTGGAACCATCGATGGCACCAAGCACTCTGCCTCAGCAGGCGCTCAGAG AAAGGTTCCACCTTCTAGGGCATCCCTGAGAAATTCCTCCAAAGTCACGGCACCAGCATCAGCAACCGTG ACATCTGCAGCTGGGACTTCCGCAGCCATATCCCGAGAGGAGAGTGGGTCTGAAGACCAATTCCTGAACA AGCGACCCAGGTTGGAGGACAGGACTGCTTTGAATAATATCAAGGAGCAAACACAGAGTGGTGGTGACCT TGAGGATAGCTCACGGCCCACAGCCATCAGCACGCCTCGGAGCTCAGGCGGTCAGCGGCGGCTGGTCAAC TGTCCAGTGTGTCAGGGCGTAGTTTTGGAGTCGCAGATTAATGAGCACTTGGACCGCTGCCTGGAAGGCA GCAAAACCAACCTCCGACCTCGAAGAGTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001106199 |
| Insert Size | 1362 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001106199.1, NP_001099669.1 |
| RefSeq Size | 1407 bp |
| RefSeq ORF | 1362 bp |
| Locus ID | 292101 |
| Gene Summary | Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR209341 | Sprtn (Myc-DDK-tagged ORF) - Rat similar to hypothetical protein (RGD1559496), (10 ug) |
CNY 5,800.00 |
|
| RR209341L3 | Lenti ORF clone of Sprtn (Myc-DDK-tagged ORF) - Rat similar to hypothetical protein (RGD1559496), (10 ug) |
CNY 7,700.00 |
|
| RR209341L4 | Lenti ORF clone of Sprtn (mGFP-tagged ORF) - Rat similar to hypothetical protein (RGD1559496), (10 ug) |
CNY 8,550.00 |
