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Asna1 (NM_001100505) Rat Untagged Clone
CAT＃: RN208953
Asna1 (untagged ORF) - Rat arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (Asna1), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN208953 representing NM_001100505
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGGGTGGCCGGGTGGGGGGTTGAAGCAGAAGAGTTCGAGGATGCACCTGACGTGGAGCCAC TGGAACCCACGCTTAGCAATATCATCGAGCAGCGTAGCCTTAAGTGGATCTTCGTCGGGGGCAAAGGAGG CGTTGGTAAGACCACCTGCAGCTGCAGCCTGGCGGTCCAGCTGTCTAAGGGACGGGAGAGTGTTCTCATC ATTTCTACAGACCCAGCTCATAACATTTCAGATGCTTTTGATCAGAAGTTCTCCAAGGTGCCTACCAAGG TCAAAGGCTATGACAACCTCTTTGCTATGGAGATAGACCCCAGCCTGGGCGTTGCAGAGCTCCCCGACGA GTTCTTCGAGGAAGACAACATGCTGAGCATGGGCAAGAAGATGATGCAGGAGGCCATGAGCGCCTTCCCT GGCATCGATGAGGCCATGAGTTATGCTGAGGTCATGAGGCTGGTGAAAGGCATGAACTTCTCAGTGGTGG TGTTCGACACAGCACCCACCGGCCATACACTCAGGCTCCTGAACTTCCCCACCATCGTGGAGCGGGGCCT GGGCCGCCTGATGCAGATCAAGAACCAGATCAGCCCCTTCATCTCACAGATGTGCAACATGCTGGGTCTG GGGGACATGAACGCTGACCAGCTGGCCTCCAAGTTAGAAGAGACCTTGCCCGTCATCCGATCCGTCAGCG AACAGTTCAAGGACCCTGAACAGACGACCTTCATCTGTGTGTGCATCGCCGAGTTTTTGTCCCTGTATGA GACGGAGCGTCTGATCCAGGAGCTGGCCAAGTGCAAGATCGACACCCACAACATCATCGTCAACCAGCTT GTCTTCCCCGACCCTGAGAAACCCTGCAAGATGTGTGAGGCCCGACACAAGATCCAGGCCAAATACCTGG ACCAGATGGAAGACCTCTATGAAGACTTTCACATTGTAAAGCTGCCACTGTTACCTCACGAGGTTCGGGG AGCCGACAAAGTCAACACCTTCTCTGCCCTCCTCCTGGAGCCCTACAAGCCCCCCAGCACCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001100505 |
| Insert Size | 1047 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001100505.1, NP_001093975.1 |
| RefSeq Size | 1365 bp |
| RefSeq ORF | 1047 bp |
| Locus ID | 288919 |
| UniProt ID | G3V9T7 |
| Gene Summary | ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR208953 | Asna1 (Myc-DDK-tagged ORF) - Rat arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (Asna1), (10 ug) |
CNY 3,990.00 |
|
| RR208953L3 | Lenti ORF clone of Asna1 (Myc-DDK-tagged ORF) - Rat arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (Asna1), (10 ug) |
CNY 6,080.00 |
|
| RR208953L4 | Lenti ORF clone of Asna1 (mGFP-tagged ORF) - Rat arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (Asna1), (10 ug) |
CNY 6,650.00 |
