Nphs1 (NM_022628) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208911 representing NM_022628 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCTAAGAGAGTCACTGTCAGAGGTGCCCGGACAAGCCCAATACACAGAACGTCCAGTTTGACTC CCCTGCTGCTCATGGGAATGCTGACCTCAGGCCTGGCCGAGTCGCCAGTCCCCACCTCAGCACCTCAAGG CTTCTGGGCTCTGTCTGAAAACCTGACTGCGGTGGAAGGGACAACAGTTAAGCTATGGTGCGGTGTCAGG GCCCCTGGCAGTGTGGTGCAGTGGGCTAAGGATGGGCTGCTTCTGGGTCCAAACCCGAAGATGCCAGGCT TCCCGAGGTACAGCCTGGAAGGAGATCGTGCTAAAGGCGAGTTCCACCTGCTTATTGAAGCCTGTGACCT CAGTGATGACGCAGAGTATGAATGCCAAGTCGGCCGCTCAGAGTTGGGTCCCGAGCTTGTGTCTCCTAAA GTAATCCTCTCCATTCTAGTTTCCCCCAAGGTGCTTCTGTTGACCCCCGAGGCAGGAAGCACAGTGACCT GGGTAGCTGGGCAGGAGTATGTGGTCACCTGTGTGTCTGGAGATGCAAAACCAGCACCTGACATCACCTT CATCCAGAGTGGACGAACTATATTGGACGTCTCCTCCAATGTGAATGAGGGATCAGAGGAGAAACTCTGC ATCACAGAGGCCGAAGCCAGGGTGATACCCCAGAGCTCGGATAACGGGCAGTTACTGGTCTGTGAGGGTT CCAACCCAGCTTTGGACACTCCCATAAAGGCTTCATTCACCATGAATATTCTGTTTCCCCCAGGACCTCC TGTCATTGATTGGCCAGGCCTGAATGAGGGGCATGTGAGGGCAGGGGAGAACCTGGAGCTGCCCTGCACA GCCAGAGGTGGCAATCCACCTGCTACCCTGCAGTGGCTGAAGAACGGTAAACCAGTGTCCACAGCCTGGG GCACCGAGCATGCCCAGGCAGTGGCCCACAGCGTGCTGGTGATGACTGTACGACCTGAAGACCATGGAGC TCGGCTCAGCTGTCAGTCCTACAACAGCGTGTCTGCAGGGACCCAGGAGAGAAGCATCACACTACAGGTC ACCTTTCCCCCAAGCGCCATTACCATCCTGGGATCTGTATCACAATCGGAGAACAAGAACGTGACCCTTT GCTGCCTGACCAAGTCCAGTCGCCCACGGGTCCTGCTGCGATGGTGGTTGGGTGGACGGCAGCTGCTGCC CACAGATGAGACAGTCATGGATGGCCTGCATGGTGGCCACATCTCCATGTCCAATCTCACATTCTTGGTG CGGAGAGAAGACAATGGCCTGCCCCTCACCTGTGAAGCCTTCAGTGACGCCTTCAGCAAGGAGACCTTCA AGAAGTCACTCACCTTGAATGTGAAATACCCTGCCCAGAAGCTGTGGATTGAGGGGCCCCCAGAGGGACA GTACATCCGGACTGGGACTCGGGTGAGGCTGGTATGCTTGGCCATCGGAGGCAACCCAGACCCCTCCCTC ATCTGGTTTAAGGATTCACGTCCGGTGAGCGAGCCCCGGCAGCCCCAGGAGCCCCGGCGTGTGCAGCTGG GCAGTGTGGAGAAGTCCGGGAGCACTTTCTCCCGCGAGCTGGTGTTGATCATAGGTCCGCCGGACAACCG AGCCAAGTTCTCCTGCAAGGCGGGTCAGCTCAGTGCGTCTACGCAGCTGGTGGTGCAGTTCCCCCCAACC AACCTGACCATCCTGGCCAACTCGTCCGCGCTGCGCCCAGGCGACGCCTTGAACTTGACCTGCGTCAGCA TCAGCAGCAACCCCCCAGTCAACTTGTCTTGGGACAAGGAAGGAGAGAGGCTGGAAGATGTGGCTGCAAA ACCCCAGAGTGCACCGTTCAAAGGCTCCGCTGCATCCAGGAGTGTTTTTCTCAGAGTGTCATCCCGAGAC CACGGTCAACGGGTCACCTGCCGGGCCCACAGCGAGGCACTCCGTGAAACCGTGAGCTCCTTCTACCGCT TCAATGTGCTGTATCCTCCAGAATTCCTGGGGGAGCAAGTCCGGGCAGTGACCGTGGTGGAGCAGGGCCA GGTGCTGCTGCCGGTGTCGGTGTCCGCTAACCCCGCCCCCGAGGCCTTCAACTGGACCTTCCGAGGCTAC CGCCTCAGCCCAGCTGGGGGTCCCCGGCACCGTATCCTGTCTGGAGGGGCTCTGCAGCTGTGGAATGTGA CCCGAGCTGACGATGGCTTTTATCAGCTGCACTGCCAGAACTCAGAGGGCACCGCTGAGGCGCTGTTGAA GCTGGACGTGCATTATGCTCCCACCATCCGTGCCCTCCGGGACCCTACTGAGGTGAATGTTGGGGGTTCT GTGGACATAGTCTGCACCGTTGACGCCAATCCCATCCTCCCAGAGATGTTCAGCTGGGAGAGACTGGGAG AAGAAGAGGAGGATCTGAACCTGGACGACATGGAGAAAGTTTCCAAGGGATCCACGGGGCGTCTGCGGAT TCGCCAAGCCAAGCTATCCCAGGCTGGTGCCTACCAGTGCATCGTGGACAATGGGGTGGCTCCTGCAGCC AGAGGACTGGTTCGTCTTGTCGTCCGATTTGCTCCCCAGGTGGATCAGCCTACTCCCCTAACAAAAGTGG CTGCCGCTGGGGACAGCACCAGCTCAGCCACACTGCACTGCCGTGCCCGGGGTGTCCCCAACATCGACTT CACTTGGACCAAAAACGGGGTCCCTCTGGATCTCCAAGACCCCAGGTACACAGAGCACAGGTACCACCAG GGTGTTGTCCACAGCAGCCTCTTGACCATCGCTAATGTGTCTGCGGCCCAGGACTATGCCCTCTTCAAAT GCACGGCCACCAATGCCCTTGGCTCTGACCACACCAACATCCAGCTCGTCAGCATCAGCCGCCCTGACCC TCCACTGGGACTGAAGGTTGTCAGCATAAGCCCTCACTCGGTGGGGCTGGAGTGGAAGCCTGGCTTTGAT GGGGGTCTGCCTCAGAGGTTCCAAATCAGGTACGAGGCCCTCGAGACCCCAGGATTCCTCCACGTGGATG TCCTACCTACACAGGCCACTACCTTCACGCTGACTGGGCTGAAGCCTTCTACACGATATAGGATCTGGCT GTTGGCCAGCAATGCCCTGGGGGACAGTGGATTGACGGACAAGGGGATCCAGGTCTCCGTCACTACCCCA GGCCCCGACCAGGCTCCTGAAGACACAGACCACCAGCTGCCCACAGAGCTGCCTCCAGGACCCCCAAGGC TGCCCCTGCTGCCTGTGCTCTTTGCAGTTGGTGGTCTTCTGCTGCTCTCCAATGCCTCCTGTGTTGGGGG TCTCCTCTGGCGGAGAAGACTGAGGCGCCTTGCTGAGGAGATCTCAGAGAAGACAGAGGCAGGGTCGGAG GACAGGATCAGGAATGAATATGAGGAGAGTCAGTGGACTGGGGACCGGGACACGAGAAGCTCCACGGTTA GCACAGCAGAAGTGGACCCAAATTACTACTCCATGAGGGACTTCAGCCCCCAGCTTCCCCCAACACTGGA GGAGGTGCTGTATCACCAAGGTGCTGAAGGCGAGGACATGGCCTTCCCCGGACACCTGCATGATGAAGTG GAGAGAGCCTATGGCCCGCCTGGGGCCTGGGGACCCCTCTATGATGAAGTACGAATGGACCCCTATGACC TTCGCTGGCCTGAGGTCCAGTGTGAGGATCCGAGGGGAATCTACGACCAGGTGGCAGCAGACATGGATGC TGTGGAAGCTAGCTCTCTGCCGTTTGAGCTGAGGGGACATCTGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_022628
Insert Size	3759 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022628.1, NP_072150.1
RefSeq Size	5820 bp
RefSeq ORF	3759 bp
Locus ID	64563
UniProt ID	Q9R044
Gene Summary	renal protein localized specifically to glomerular capillary walls; may contribute to the development of proteinuria in renal disease [RGD, Feb 2006]

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