Slc32a1 (NM_031782) Rat Untagged Clone

CAT#: RN208539

Slc32a1 (untagged ORF) - Rat solute carrier family 32 (GABA vesicular transporter), member 1 (Slc32a1), (10 ug)



  "NM_031782" in other vectors (3)

CNY 5,872.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-Vesicular GABA Transporter
    • 50 ul

CNY 11,000.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Vgat; Viaat
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN208539 representing NM_031782
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCCTGCTCCGCAGCAAGCTGACCAACGTGGCCACCTCTGTGTCCAACAAGTCCCAGGCCAAGG
TGAGCGGCATGTTCGCCAGGATGGGGTTTCAGGCGGCCACGGATGAGGAGGCGGTGGGCTTCGCGCACTG
CGACGATCTCGACTTTGAGCACCGCCAGGGCCTGCAGATGGACATCCTGAAATCGGAAGGCGAGCCCTGC
GGGGACGAGGGCGCAGAACCTCCCGTCGAGGGAGACATTCATTATCAGCGCGGCGGCGCTCCCCTGCCAC
CCTCGGGCTCCAAGGACCAGGCCGTGGGAGCTGGTGGGGAGTTCGGGGGTCACGACAAACCCAAGATCAC
GGCGTGGGAAGCGGGCTGGAACGTGACAAACGCCATTCAGGGCATGTTCGTGCTGGGTCTACCCTACGCC
ATCCTCCACGGCGGCTACCTGGGGTTGTTCCTCATCATCTTCGCCGCGGTGGTGTGCTGCTACACCGGCA
AGATCCTCATCGCGTGCCTGTACGAGGAGAACGAAGATGGTGAGGTGGTGCGCGTGAGGGACTCGTATGT
GGCCATAGCTAACGCGTGCTGCGCTCCTCGATTCCCCACGCTGGGCGGCCGCGTGGTCAATGTGGCCCAG
ATCATCGAGCTGGTGATGACGTGTATCTTGTACGTAGTGGTGAGCGGCAACCTCATGTACAACAGTTTCC
CGGGGCTGCCCGTGTCGCAGAAGTCCTGGTCCATCATAGCCACGGCGGTGCTGCTGCCCTGCGCCTTCCT
GAAGAATCTCAAGGCCGTGTCCAAGTTCAGTCTGCTGTGCACGCTGGCCCACTTCGTCATCAACATCCTG
GTCATCGCCTACTGTCTCTCGCGCGCGCGTGACTGGGCCTGGGAGAAGGTGAAGTTCTACATCGACGTCA
AGAAGTTTCCTATCTCCATCGGCATCATCGTGTTCAGCTACACGTCGCAGATCTTCCTGCCCTCGCTCGA
AGGCAACATGCAGCAGCCCAGCGAATTCCACTGCATGATGAACTGGACACACATCGCCGCCTGCGTGCTC
AAGGGTCTCTTCGCGCTCGTCGCCTACCTCACCTGGGCCGACGAGACCAAGGAAGTCATCACGGATAACC
TGCCCGGTTCCATCCGCGCCGTGGTCAACATCTTCCTGGTGGCCAAGGCGCTGCTGTCCTACCCGTTGCC
CTTCTTCGCGGCCGTCGAAGTGCTGGAGAAGTCTCTCTTCCAGGAAGGCAGTCGTGCCTTCTTCCCCGCC
TGCTACGGTGGCGACGGTCGCCTTAAGTCCTGGGGGCTGACGCTGCGCTGCGCGCTGGTGGTCTTCACGC
TGCTCATGGCCATCTACGTGCCACACTTCGCGCTGCTCATGGGCCTCACGGGCAGCCTCACGGGAGCCGG
CCTCTGCTTCCTGCTGCCCAGCCTCTTCCACTTGCGTCTTCTCTGGCGCAAGCTGCTGTGGCACCAGGTC
TTCTTCGATGTGGCCATCTTCGTCATCGGCGGCATCTGCAGCGTGTCCGGCTTCGTGCATTCACTCGAGG
GCCTCATCGAGGCCTACCGAACCAACGCAGAGGACTAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII     
ACCN NM_031782
Insert Size 1578 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_031782.1, NP_113970.1
RefSeq Size 2392 bp
RefSeq ORF 1578 bp
Locus ID 83612
UniProt ID O35458
Gene Summary integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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