Wnk4 (NM_175579) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ac2-059; Prkwnk4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208361 representing NM_175579 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGCACCTCGAAATACAGAGACCGGGGTCCACATGTCCCAAACGGAGGCCGACCTGGCTTTGCGGC CATCTCCGTCTCTCACCTCCATGGGGCCGACCCGCCTGGGGCCACCTCCTCGCCGAGTGCGCCGCTTCTC TGGGAAGGCTGAGCCCCGGCCGCGCTCTTCGAGACCGAGCCGCCGCAGCTCAGTCGATCTGGGACTGTTG AGCTCCTGGTCTCAACCAGCCTCACTCCTTCCGGAACCTCCTGATCCTCCGGACTCCGCTGGTCCCATGA GGAGCCCACCTTCAAACTCTAAAGAACACCCCGAGGGCACATGGACGGGGGCAGCCCCGGTGAAGGCTGC AGACTCTGCCTGTCCTGAGCTTACAGTATCTTCCGGAGGTCCGGGGTCCCGGGAGCCACCAAGGGTTCCT GATGCTGCAGCCCGGGAACGGCGGCGGGAGCAGGAAGAAAAAGAGGACACAGAGACCCAGGCTGTGGCAA CGTCTCCCGACGGCCGATACCTCAAGTTTGACATTGAGATTGGAAGAGGTTCCTTCAAGACCGTGTATCG AGGGCTAGACACCGACACCACGGTAGAGGTGGCCTGGTGTGAGCTGCAGGCTCGGAAACTCTCTCGGGCT GAACGGCAGCGTTTCTCTGAGGAAGTTGAGATGCTCAAGGGGCTGCAGCACCCCAACATCGTCCGCTTCT ATGACTCCTGGAAGTCGGTGCTGAGGGGTCAGGTTTGCATCGTGCTGGTCACAGAACTCATGACCTCGGG CACGCTCAAGACGTACCTGAGGAGATTCCGAGAAATGAAGCCCCGAGTCCTTCAGCGCTGGAGTCGCCAG ATCCTACGGGGACTTCATTTCTTACATTCCCGAGTGCCCCCCATCCTGCACCGAGATCTCAAATGTGACA ATGTCTTCATTACCGGCCCTTCAGGCTCTGTCAAAATCGGAGATCTCGGACTGGCCACGCTCAAGCGCGC CTCCTTTGCCAAGAGCGTCATTGGGACCCCGGAATTCATGGCCCCTGAGATGTATGAGGAAAAGTACGAC GAGGCTGTGGATGTGTACGCCTTTGGCATGTGCATGCTGGAGATGGCTACGTCTGAGTATCCCTACTCTG AGTGTCAGAACGCAGCACAAATCTACCGAAAGGTCACTTCGGGCACAAAGCCCAACAGCTTCTACAAAGT GAAGATGCCAGAGGTGAAGGAGATCATTGAAGGCTGCATCCGCACGGATAAGAATGAGAGGTTCACTATC CAGGATCTTCTGACCCACGCGTTCTTCCGCGAGGAACGTGGTGTGCATGTGGAGCTGGCAGAGGAAGATG ATGGAGAGAAGCCGGGTCTCAAACTCTGGCTGCGCATGGAGGACGCACGACGCGGGGGGCGCCCAAGGGA CAACCAGGCCATCGAGTTCCTGTTCCAGCTCGGTCGGGATGCAGCGGAAGAGGTGGCTCAGGAGATGGTA GCCTTGGGCTTAGTCTGCGAAGCAGACTACCAGCCAGTGGCCCGTGCAGTTCGAGAACGGGTTGCTGCCA TCCAGCGGAAGCGGGAGAAGCTAAGAAAAGCCAGGGAATTGGAGGTTCTCCCACCAGACTCAGGACCTCC TCCAGCAACTGTGTCTATGACTCCTGGTCCCCCCAGTGCCTTCCCCCCAGAGCCCGAGGAGCCAGAGGCT GACCAGCACCAATCCTTCCTCTTCCGCCATGCCAGCTACTCATCCACTACATCTGATTGCGAGACTGATG GCTACCTCAGCTCCTCCGGCTTCCTGGATGCCTCAGACCCTGCCCTTCAGCCCCCCGGGGGGATGCCATC CAGCCCCGCTGAGCCCCATCTCTGCTTGCCCTCGGGTTTTGCCTTGTCCATTCCACGGTCTGGCCCTGGC AGTGACTTTTCTCCTGGGGACAGCTACGCCTCAGATGCAGCCTCAGGCCTCAGTGACATGGGAGAAGGGG GGCAAATGAGGAAAAATCCAGTGAAGACTCTGCGACGGAGACCTCGATCCCGGCTTCGGGTCACGAGTGT CTCAGACCAGAGCGACAGAGTCGTGGAGTGCCAGCTGCAAACTCACAACAGCAAGATGGTGACGTTCCGA TTTGATCTGGATGGGGACAGCCCAGAAGAAATCGCAGCTGCCATGGTTTATAATGAATTCATTCTGCCCT CGGAGCGAGACGGATTCCTGAGCCGGATCCGGGAGATTATCCAGCGAGTGGAGACCCTGCTGAAGAGAGA TGCTGGTCCCTCGGAGGCCACTGAAGATGCGCTGAGCCCTCAGGAAGAGGCAGCAGCCATGCCTGCCCTC CCAGGCCCATCCGATGAAGAGCTCCAGAGAAGCATCTCCCCGGAACAGAGGAGCTGGGCAGCCTTCTCTA CATCTCCATCTTCTCCTGGCACCCCCTTGTCCCCTGGAACCCCCTTTTCCCCTGGGACCCCTCCTGTCTT CCCATGCCCCATCTTCCCTATCACTTCACCCTCATGCCATCCCTACCCGTTCTCACAGGTCTCTTCAAAT CCCTGTCCACAGGCCCCCAGCTCCCTACTTCCCTCCTCCTCCGGTGCTTCTCAGGTTCCATTCCCATCCC CTTCCCTTCCCACCAGCTCTCCCCTCCCATTCTCTCCTAGTTATCCCCAAGTCCCTCTTCACCCTGCTTC TCTTCCCACCTGCCCCTCCCCTCCCCCTCTCCCCTCCACCACAGCAGCCCCTCTCCTCTCTCTGGCTAGT GCCTTCTCTCTGGCTGTGATGACTGTGGCCCAGTCCCTGCTGTCCCCATCCCCTGGGCTTCTCTCTCAGT CTCCTCCAGCCCCTCCAGGTCCTCTGCCTAGCATGCCCCTTCCCCTTGCTTCTTGTGACCAGGAGAGCCT TTCAGCCCAAACAGCTGAGACAGAGAATGAGGCTTCCCGAAATCCTGCTCAGCCACTACTGGGTGATGCT AGACTGGCACCCATATCTGAAGAGGGAAAACCCCAGCTGGTTGGCCGATTCCAAGTGACTTCATCCAAGG AACCAGCAGAGCCTCCCCTACAACCAGCATCTCCAACTCTCTCCAGATCCCTAAAGCTTCCAACCCCTCA GCTGACCTCAGAGAGTTCAGACACAGAGGACAGTGCTGCAGGAGGACCAGAGACCAGGGAGGCTCTGGCA GAGAGTGACCGTGCAGCCGAGGGCTTGGGGGTCGCCATCGATGAGGAAAAGGATGAAGGGAAGGAACCCC AAATTGGGGGCAGTTCCCCAATCTTGAGCCAGCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCT GTGTCTAAGCAGTGAGGAGTCAGAGAGCAGCGGGGAGGACGAGGAGTTCTGGGCTGAGCTGCAGAACCTT CGGCAGAAGCACTTGTCGGAAGTGGAAGCACTACAGACGCTACAGAAGAAGGAAATCGAAGACTTGTACA GCCGGCTTGGAAAGCAACCCCCACCTGGTATTGTAGCTCCAGCGGCTATGCTGTCCTGCCGCCAGCGCCG CCTCTCCAAGGGAAGCTTTCCCACCTCCCGCCGCAACAGCCTGCAGCGCTCTGATCTCCCTGGCCCTGGC ATCATGCGGAAGAACTCTCTGAGTGGCAGCAGCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGA CATTCGCCGGGGATGTTGGCAGGATGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_175579
Insert Size	3669 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175579.3, NP_783169.2
RefSeq Size	4093 bp
RefSeq ORF	3669 bp
Locus ID	287715
UniProt ID	Q7TPK6
Gene Summary	mutation of the human homolog causes pseudohypoaldosteronism type II [RGD, Feb 2006]

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