United States
Germany
Japan
United Kingdom
China
Ndrg1 (NM_001011991) Rat Untagged Clone
CAT＃: RN208229
Ndrg1 (untagged ORF) - Rat N-myc downstream regulated gene 1 (Ndrg1), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ndr1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN208229 representing NM_001011991
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGAGAGCTACATGACGTGGACCTCGCCGAGGTGAAGCCTCTGGTGGAAAAGGGGGAGAGCATCA CTGGTCTCCTGCAAGAGTTCGATGTTCAGGAGCAGGACATTGAGACCCTACATGGCTCCCTGCATGTCAC TCTGTGTGGAACCCCCAAGGGCAACCGTCCTGTCATCCTCACGTATCACGACATCGGCATGAACCACAAG ACCTGCTACAACCCTCTCTTCAACTCTGAGGATATGCAAGAGATCACACAACATTTTGCGGTCTGCCATG TGGATGCCCCTGGCCAACAGGATGGTGCCCCTTCCTTCCCAGTGGGGTACATGTATCCCTCAATGGATCA GTTGGCTGAAATGCTTCCTGGAGTCCTTCACAAGTTTGGGCTTAAGAGTGTCATTGGCATGGGGACAGGA GCTGGCGCCTACATCCTGACCCGCTTTGCACTCAACAACCCTGAGATGGTGGAGGGCCTTGTGCTCATGA ATGTGAATCCCTGTGCTGAAGGCTGGATGGACTGGGCTGCCTCCAAGATCTCAGGATGGACCCAAGCCCT GCCAGACATGGTGGTGTCCCACCTCTTCGGCAAGGAGGAGATACACAGCAATGTGGAGGTGGTGCACACG TACCGCCAGCACATCCTCAACGACATGAACCCGAGCAACCTGCACCTGTTCATCAGCGCCTACAACAGCC GGAGAGACCTGGAGATTGAGCGGCCAATGCCTGGAACCCACACTGTCACCCTGCAGTGCCCTGCTCTGCT CGTGGTTGGAGACAACTCTCCTGCCGTGGATGCTGTGGTGGAATGCAATTCCAAATTGGACCCAACGAAG ACCACCCTACTCAAGATGGCAGACTGTGGAGGCCTTCCTCAGATCTCTCAGCCTGCCAAGCTTGCAGAGG CCTTCAAGTACTTTGTGCAGGGCATGGGATACATGCCTTCTGCCAGCATGACTCGCCTGATGCGGTCCCG CACAGCCTCTGGCTCCAGTGTCACATCCCTGGAGGGCACCCGTAGCCGCTCCCATACCAGCGAGGGGCCC CGAAGTCGCTCACACACAAGTGAGGGGTCCCGTAGCCGCTCCCATACCAGTGAGGATGCCCGTCTGAACA TCACCCCCAGCTCGGGTGCCACAGGGAACAATGCCGGGCCCAAGTCCATGGAGGTGTCCTGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001011991 |
| Insert Size | 1185 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001011991.1, NP_001011991.1 |
| RefSeq Size | 2906 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 299923 |
| UniProt ID | Q6JE36 |
| Gene Summary | Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking notably of the Schwann cell and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR208229 | Ndrg1 (Myc-DDK-tagged ORF) - Rat N-myc downstream regulated gene 1 (Ndrg1), (10 ug) |
CNY 5,488.00 |
|
| RR208229L3 | Lenti ORF clone of Ndrg1 (Myc-DDK-tagged ORF) - Rat N-myc downstream regulated gene 1 (Ndrg1), (10 ug) |
CNY 6,080.00 |
|
| RR208229L4 | Lenti ORF clone of Ndrg1 (mGFP-tagged ORF) - Rat N-myc downstream regulated gene 1 (Ndrg1), (10 ug) |
CNY 6,650.00 |
