Med28 (NM_001107217) Rat Untagged Clone

CAT#: RN207753

Med28 (untagged ORF) - Rat mediator complex subunit 28 (Med28), (10 ug)



  "NM_001107217" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Eg1; FKSG20; RGD1305875
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN207753 representing NM_001107217
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGTCCCTGGGAGGGATGTTCGCCGGCCAGCCTCCAGGACCTCCTCCGCCACCGCCCGGGCTCC
CAGGCCAGGCGTCGCTTCTACAAGCAGCCCCTGGGGCTCCGAGACCATCTAACAGCACTTTGGTGGACGA
GTTGGAATCATCATTCGAGGCTTGCTTTGCTTCCCTTGTGAGTCAGGATTATGTCAATGGGACTGATCAG
GAAGAAATTCGAACTGGTGTTGATCAGTGTATCCAGAAGTTTTTGGACATTGCAAGACAGACAGAATGCT
TTTTCTTACAAAAAAGGTTGCAATTGTCTGTCCAGAAACCAGATCAAGTTATCAAAGAGGATGTCTCAGA
ACTAAGGAGTGAATTGCAGCGGAAAGATGCTCTGGTCCAGAAGCACTTGACAAAACTGAGACATTGGCAG
CAGGTGCTGGAAGACATCAATGTGCAGCACAAGAAGCCAGCCGACATGCCTCAGGGCTCCTTGGCCTACC
TTGAGCAGGCATCTGCCAACATACCTGCACCTCTGAAGCAGACCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001107217
Insert Size 537 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001107217.1, NP_001100687.1
RefSeq Size 2089 bp
RefSeq ORF 537 bp
Locus ID 305391
UniProt ID P68943
Gene Summary May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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