Grin2b (NM_012574) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	GluN2B
Vector	pCMV6-AC-Myc-DDK
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207651 representing NM_012574 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCCAGCGCAGAGTGCTGTTCCCCCAAGTTCTGGTTGGTGTTGGCCGTCTTGGCCGTATCAGGCA GCAAAGCTCGTTCCCAAAAGAGCCCCCCCAGCATCGGCATCGCTGTCATCCTCGTGGGCACTTCAGACGA AGTGGCCATAAAAGACGCCCACGAGAAAGATGACTTCCATCATCTCTCAGTAGTTCCCCGGGTGGAGCTG GTAGCCATGAACGAAACTGACCCAAAGAGCATCATCACCCGTATCTGCGATCTTATGTCTGACCGGAAGA TCCAGGGGGTGGTGTTCGCGGATGACACCGACCAAGAAGCCATCGCTCAGATCCTCGACTTCATTTCTGC TCAGACTCTCACCCCCATCCTGGGCATCCATGGGGGCTCATCTATGATAATGGCGGATAAGGATGAGTCC TCCATGTTCTTCCAGTTTGGCCCGTCTATCGAACAGCAAGCTTCCGTCATGCTCAACATCATGGAAGAAT ATGACTGGTACATCTTTTCCATCGTCACCACCTACTTCCCTGGCTACCAGGACTTTGTGAACAAGATCCG CAGTACCATCGAGAACAGCTTCGTGGGCTGGGAGCTCGAGGAAGTCCTCCTGCTAGACATGTCTCTGGAC GATGGCGACTCTAAGATTCAGAATCAGCTGAAGAAGCTCCAAAGCCCCATCATTCTCCTTTATTGCACGA AGGAGGAAGCCACCTACATTTTTGAAGTAGCTAACTCAGTTGGGCTGACTGGCTACGGCTACACGTGGAT TGTGCCGAGTCTGGTGGCCGGGGATACGGACACGGTGCCTTCAGAGTTCCCCACGGGGCTTATCTCTGTG TCTTATGATGAATGGGACTATGGCCTTCCTGCCAGAGTGAGAGATGGAATTGCCATCATCACCACTGCTG CCTCGGACATGCTGTCCGAACACAGTTTCATCCCTGAGCCCAAGAGCAGTTGCTACAACACCCACGAGAA GAGGATCTACCAGTCTAACATGTTGAATAGGTATCTGATCAATGTCACTTTTGAAGGGAGAAACCTGTCC TTCAGCGAAGATGGCTACCAGATGCATCCGAAGCTGGTGATAATCCTTCTGAACAAGGAGAGGAAGTGGG AGAGGGTGGGGAAATGGAAGGACAAGTCCCTGCAGATGAAGTATTATGTGTGGCCTCGGATGTGTCCTGA GACTGAGGAGCAAGAGGATGACCATCTGAGCATTGTCACCTTGGAGGAGGCGCCATTTGTCATTGTGGAA AGCGTGGACCCTCTCAGTGGAACCTGCATGAGGAATACAGTCCCGTGCCAGAAGCGCATCATCTCTGAGA ATAAAACAGATGAGGAACCAGGCTACATCAAAAAATGCTGCAAGGGGTTCTGTATTGACATCCTTAAGAA AATTTCTAAGTCTGTGAAGTTCACCTATGACCTTTACCTGGTGACCAATGGCAAGCACGGGAAGAAGATT AATGGGACCTGGAATGGCATGATCGGTGAGGTGGTCATGAAGAGGGCCTACATGGCAGTGGGATCACTAA CTATCAATGAAGAACGGTCAGAGGTGGTTGACTTCTCTGTACCCTTCATAGAAACTGGCATCAGTGTCAT GGTATCTCGCAGCAATGGGACTGTGTCACCTTCTGCCTTCTTAGAGCCATTCAGCGCTGACGTGTGGGTG ATGATGTTTGTGATGCTGCTCATTGTTTCTGCGGTGGCTGTCTTTGTCTTTGAATACTTCAGCCCTGTGG GTTACAACAGGTGCCTAGCCGATGGCAGAGAGCCAGGAGGCCCATCTTTCACCATCGGCAAAGCAATTTG GTTACTCTGGGGTCTGGTGTTTAACAACTCCGTACCTGTGCAGAACCCAAAGGGGACCACCTCCAAGATC ATGGTGTCAGTGTGGGCCTTCTTTGCTGTCATTTTCCTGGCCAGCTACACTGCCAACTTAGCAGCCTTCA TGATCCAAGAGGAGTATGTGGACCAGGTTTCTGGCCTGAGTGACAAGAAGTTCCAGAGACCTAATGACTT CTCACCCCCTTTCCGCTTTGGGACTGTGCCCAATGGCAGCACAGAGAGGAATATCCGTAATAACTATGCA GAAATGCATGCCTACATGGGAAAGTTCAACCAAAGGGGTGTAGATGATGCATTGCTCTCCCTGAAAACAG GGAAGCTTGATGCATTCATCTATGATGCAGCTGTGCTCAACTACATGGCTGGAAGGGACGAAGGCTGCAA ACTGGTGACCATTGGCAGTGGCAAGGTCTTTGCTTCTACCGGCTATGGCATTGCTATCCAAAAGGACTCC GGGTGGAAGCGCCAGGTGGACCTGGCTATCCTGCAGCTGTTTGGAGATGGGGAGATGGAAGAACTGGAAG CTCTCTGGCTCACTGGCATTTGCCACAATGAGAAGAATGAGGTGATGAGCAGCCAGCTGGACATCGACAA TATGGCAGGTGTCTTCTATATGTTGGGGGCAGCCATGGCCCTCAGCCTCATCACCTTCATCTGTGAGCAT CTGTTCTATTGGCAGTTCCGGCATTGCTTCATGGGTGTCTGTTCTGGCAAGCCTGGCATGGTCTTCTCCA TCAGCAGAGGTATCTACAGCTGTATCCATGGGGTAGCCATAGAGGAGCGCCAATCCGTGATGAACTCCCC CACTGCCACCATGAACAACACCCACTCCAACATCCTACGCTTGCTCCGCACGGCCAAGAACATGGCCAAC CTGTCTGGAGTAAACGGCTCCCCTCAGAGTGCCCTGGACTTCATCCGCCGAGAGTCCTCCGTCTACGACA TCTCTGAGCATCGTCGCAGCTTCACGCATTCAGACTGCAAGTCTTACAATAACCCACCCTGTGAGGAAAA CCTGTTCAGTGACTACATTAGCGAGGTAGAGAGAACATTTGGTAACCTGCAGCTGAAGGACAGCAATGTG TACCAAGACCACTATCACCATCACCACCGGCCACACAGCATCGGCAGCACCAGCTCCATTGATGGGCTCT ATGACTGTGACAACCCACCCTTCACCACCCAGCCCAGGTCAATCAGCAAGAAACCCCTGGACATCGGCCT GCCCTCCTCCAAACATAGCCAGCTCAGCGACCTGTATGGCAAGTTCTCTTTCAAGAGTGACCGCTACAGT GGCCACGACGACTTGATTCGATCGGATGTCTCCGACATCTCCACGCACACTGTCACCTATGGGAACATCG AGGGCAACGCAGCCAAGAGGAGGAAACAGCAGTATAAGGACAGTCTAAAGAAGCGGCCAGCCTCGGCCAA ATCGAGGAGGGAGTTTGATGAAATCGAGCTGGCCTACCGTCGCCGACCACCCCGCTCCCCGGACCACAAG CGCTACTTCAGGGACAAAGAAGGGCTCCGAGACTTCTACCTGGACCAGTTCCGAACAAAGGAGAACTCGC CTCACTGGGAGCACGTGGACTTGACTGACATTTACAAAGAACGCAGTGACGACTTCAAGCGAGATTCGGT CAGTGGAGGTGGGCCCTGTACCAACAGGTCTCACCTCAAACACGGAACGGGCGAGAAGCACGGAGTGGTA GGCGGGGTGCCTGCTCCTTGGGAGAAGAACCTGACCAATGTGGATTGGGAGGACCGGTCTGGGGGCAACT TCTGCCGCAGCTGTCCTTCCAAGCTGCACAATTACTCCTCGACGGTGGCAGGGCAGAACTCGGGCCGGCA GGCCTGCATCAGATGTGAGGCCTGTAAGAAGGCTGGTAACCTGTACGACATCAGCAAGGACAACTCCCTG CAGGAACTGGACCAGCCGGCTGCCCCCGTGGCTGTGACATCCAACGCCTCCAGCACCAAGTACCCTCAAA GCCCGACTAATTCCAAGGCTCAGAAGAAGAATCGGAACAAACTGCGCCGGCAGCATTCCTACGACACCTT CGTGGACCTGCAGAAGGAGGAGGCCGCCTTGGCCCCACGCAGCGTGAGCCTGAAAGACAAGGGCCGATTC ATGGATGGGAGCCCCTACGCCCATATGTTTGAGATGCCAGCTGGTGAGAGCTCCTTTGCCAACAAGTCCT CAGTGCCCACTGCCGGACACCACCACAACAACCCCGGCAGCGGCTACATGCTCAGCAAGTCGCTCTACCC TGACCGGGTCACGCAAAACCCTTTCATCCCCACTTTTGGGGATGACCAGTGCTTGCTTCACGGCAGCAAA TCCTACTTCTTCAGGCAGCCCACGGTGGCAGGGGCGTCAAAAACAAGGCCGGACTTCCGGGCCCTTGTCA CCAATAAGCCAGTGGTGTCAGCCCTTCATGGGGCTGTGCCAGGTCGTTTCCAGAAGGACATTTGTATAGG GAACCAGTCCAACCCCTGTGTGCCTAACAACAAAAACCCCAGGGCTTTCAATGGCTCCAGCAATGGACAT GTTTATGAGAAACTTTCTAGTATTGAGTCTGATGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_012574
Insert Size	4449 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012574.1, NP_036706.1
RefSeq Size	5259 bp
RefSeq ORF	4449 bp
Locus ID	24410
UniProt ID	Q00960
Gene Summary	subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

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