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Atg7 (NM_001012097) Rat Untagged Clone
CAT＃: RN207526
Atg7 (untagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug)
CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Apg7l |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207526 representing NM_001012097
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACCCTGGACTGTCCAAGCTGCAGTTTGCCCCCTTTAATAGTGCCCTGGACGTTGGCTTCTGGC ACGAACTGACCCAGAAGAAGTTGAACGAGTATCGCCTGGACGAGGCACCCAAAGACATCAAGGGCTATTA CTACAATGGTGACTCTGCCGGCCTCCCCACCCGCTTGACATTGGAGTTCAGTGCTTTTGACATGAGCGCC CCCACGCCTGCCCGCTGCTGCCCGGCCATGGGAACCCTGCACAACACCAACACACTTGAGGCTTTTAAGA CTGCAGACAAGAAGCTCCTTCTGGAGCAGTCAGCCAATGAGATCTGGGAAGCCATAAAGTCAGGTGCTGC TCTCGAAAACCCCATGCTCCTCAACAAGTTTCTGCTCTTGACCTTCGCGGACCTAAAGAAGTACCACTTC TACTACTGGTTTTGCTGCCCTGCCCTCTGTCTTCCTGAGAGCATCCCTCTAATCCGGGGACCTGTGGGCT TGGATCAAAGGCTTTCACCAAAACAGATCCAGGCCCTGGAGCATGCCTACGATGACCTGTGTCGAACTGA AGGCGTCACAGCCCTGCCATACTTCTTATTCAAGTACGATGACGACACTGTGCTGGTCTCCTTGCTCAAA CACTACAGTGATTTCTTCCAAGGTCAAAGGACAAAGTTAACAGTCGGTGTGTACGATCCCTGTAACCTAA CCCAGCACCCTGGATGGCCTTTGAGGAATTTTTTGGTCCTGGCAGCCCACAGATGGAGCGGCAGTTTCCA GTCTGTTGAAGTTCTCTGCTTTCGGGACCGCACCATGCAGGGAGCAAGAGATGTGACACACAGCATCATC TTTGAAGTGAAACTTCCAGAAATGGCGTTTAGCCCAGATTGTCCTAAGGCAGTTGGCTGGGAGAAGAACC AGAAAGGAGGCATGGGTCCGAGGATGGTGAACCTCAGCGGATGTATGGACCCCAAAAGGCTGGCTGAGTC ATCCGTGGATCTGAATCTCAAGCTGATGTGCTGGCGGTTGGTCCCCACCTTGGACTTGGACAAGGTCGTG TCTGTCAAGTGCCTGCTGCTGGGAGCTGGTACCTTGGGGTGTAATGTGGCTAGAACACTGATGGGCTGGG GCGTCAGACACGTCACGTTTGTGGACAACGCCAAGATCTCCTACTCCAATCCCGTGAGGCAGCCTCTGTA TGAATTTGAAGATTGTCTAGGGGGTGGCAAGCCCAAGGCCCTGGCTGCAGCAGAGCGGCTCCAGAAAATA TTTCCCGGAGTGAATGCCAGCGGGTTCAACATGAGCATCCCCATGCCCGGACACCCTGTGAACTTCTCTG ACGTCACGATGGAGCAGGCCCGCAGAGATGTGGAGCAGCTGGAGGAACTCATCGATAGCCACGATGTCAT CTTCCTGCTAATGGACACCAGGGAGAGCCGATGGCTTCCTACTGTTATTGCAGCCAGCAAGCGAAAGCTG GTCATCAATGCTGCCTTGGGGTTTGACACCTTTGTTGTCATGAGACATGGCCTGAAGAAACCCAAGCAGC AGGGAGCCGGGGACCTGTGCCCAAGCCATCTTGTAGCACCTGCTGACCTGGGCTCCTCACTTTTTGCCAA CATCCCTGGATACAAGCTTGGCTGCTACTTCTGCAATGATGTGGTGGCTCCAGGAGATTCCACCAGAGAC CGGACTCTGGACCAGCAGTGCACAGTGAGCCGCCCAGGGCTGGCCGTGATTGCAGGAGCCCTGGCTGTGG AGCTGATGGTTTCTGTCTTGCAGCATCCTGAGGGGGGCTACGCCATCGCCAGCAGCAGTGATGACCGCAT GAATGAGCCTCCCACCTCGCTGGGGCTTGTACCTCACCAGATCCGGGGCTTTCTGTCACGGTTCGATAAT GTCCTTCCTGTCAGCCTGGCATTTGATAAATGTACAGCCTGTTCATCCAAAGTTCTTGATCAATATGAGC AAGAAGGATTCACCTTCCTGGCCAAGGTGTTTAACTCTTCACATTCCTTCTTAGAAGATTTGACTGGTCT TACACTGCTCCACCAAGAGACCCAAGCTGCTGAGATCTGGGACATGAGTGACGAGGAGACCGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001012097 |
| Insert Size | 2097 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001012097.1, NP_001012097.1 |
| RefSeq Size | 2386 bp |
| RefSeq ORF | 2097 bp |
| Locus ID | 312647 |
| UniProt ID | Q641Y5 |
| Gene Summary | E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation (By similarity). Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207526 | Atg7 (Myc-DDK-tagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug) |
CNY 8,930.00 |
|
| RR207526L3 | Lenti ORF clone of Atg7 (Myc-DDK-tagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug) |
CNY 10,740.00 |
|
| RR207526L4 | Lenti ORF clone of Atg7 (mGFP-tagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug) |
CNY 11,880.00 |
